Mouse Neuron Population Database Retinal Ganglion Cell Estimates "Version of Feb 2, 1996 (last version posted on WWW was Oct 10, 1995, n=450)" "Robert W. Williams, Richelle C. Strom, Dennis S. Rice, and Dan Goldowitz" Address questions and comments to rwilliams@uthsc.edu "With thanks to R. Cushing, S. Smith, B. Taylor, M. Davisson, and T. K. Hurt" number of independent samples 763 2460 number of individuals counted 573 "55,178" CV =SD/Mean "number cases, category 1" 540.00146 "2,449" 2.400 number cases included in first set of studies 451.00000 "Submitted Dec 13, 1995" number of types of mice 62 Dec 12 F2 Stats N = 54 cases total number of standard inbred strains 17 Criterion N mean SD SEM number of F1 cross types 5 ">65,000" 12 70331 "4,094" "1,234" number of BXD strains 26 "<50,000" 15 47082 "2,191" 585 number of BXH strains 12 number of wild inbred strains 7 number of non-inbred strains 3 number of N2 types 2 number of other strains 2 number of duplicate counts 146 number of R vs L nerve comparisons in study 19 "Multipliers of the range to get unbiased SD: n=2: x0.886, 3: 0.591, 4: 0.486, 5: 0.430, 6: .395, 7: 0.370, 8: 0.351, 9: 0.337, 10: 0.325, 11: 0.315, 12: 0.307, 13: 0.300, 14: 0.294, 15: 0.288, 16: 0.283, 17: 0.279, 18: 0.275, 19: 0.271, 20: 0.268 " average #DIV/0! ALL INBRED non-wild "t dist, 2-tailed, p=0.025 per tail)" See Table A6 of Snedecor and Cochran (1980) For Bartlett test of homogeneity of variance For Bartlett test of homogeneity of variance For Bartlett test of homogeneity of variance For Bartlett test of homogeneity of variance For Bartlett test of homogeneity of variance Strain or Species Neurons SEM SD CV N 95%CI- 95%CI+ Lowest Highest Range SD_Range Z Age Body Brain BrainRGC Corr Retina H_cells ±HC RGC/HC H or L type note Rank_RGC Z_Fract Z_Exp RGCs Rank_Brain Z_Fract_Brain RGC-Brain Correl Z_Exp_Brain Brain VAR LN VAR df df*LN(VAR) 1/df 129/J "63,772" "±1,699" "±4,163" 6.5% 7 "60,441" "67,103" "57,185" "68,409" "11,223" "±4,153" 0.40 76 20.0 407 16.5 ? inbred c/c 3 in prog 31 0.622 0.312 "63,772" 13 0.255 -0.674 -0.659 407 " 17,328,934 " 16.7 6 100 0.167 A/J "51,238" "±1,362" "±3,602" 7.0% 8 "48,569" "53,906" "46,312" "56,720" "10,408" "±3,653" -1.63 111 22.2 395 14.0 "7,873" ±114 6.5 L inbred done 1 0.010 -2.319 "51,238" 6 0.112 0.023 -1.215 395 " 12,976,709 " 16.4 7 115 0.143 AKR/J "62,788" ±890 "±1,990" 3.2% 6 "61,044" "64,532" "60,063" "65,143" "5,081" "±2,007" 0.24 71 - 449 14.7 "11,398" ±510 5.5 H inbred c/c done 26 0.520 0.051 "62,788" 37 0.745 0.210 0.659 449 " 3,959,370 " 15.2 5 76 0.200 BALB/cBy "57,558" "±1,768" "±4,330" 7.5% 7 "54,094" "61,023" "51,320" "63,924" "12,604" "±4,663" -0.61 30 14.4 403 - ? inbred c/c 3 in progress 17 0.337 -0.421 "57,558" 11 0.214 -0.629 -0.792 403 " 18,745,774 " 16.7 6 100 0.167 BALB/cJ "63,393" "±2,210" "±5,846" 9.2% 8 "59,062" "67,724" "53,252" "68,788" "15,536" "±5,453" 0.34 96 23.9 477 15.3 H inbred c/c done 28 0.561 0.154 "63,393" 46 0.929 0.208 1.465 477 " 34,174,206 " 17.3 7 121 0.143 BXD1 "61,996" "±1,077" "±3,231" 5.2% 10 "59,885" "64,107" "59,552" "67,167" "7,615" "±2,566" 0.11 51 20.5 448 22 0.439 -0.154 "61,996" 35 0.704 0.120 0.536 448 " 10,441,458 " 16.2 9 145 0.111 BXD2 "66,063" "±1,644" "±3,677" 5.6% 6 "62,840" "69,286" "60,417" "70,774" "10,358" "±4,091" 0.77 53 23.1 427 40 0.806 0.864 "66,063" 22 0.439 0.763 -0.154 427 " 13,521,443 " 16.4 5 82 0.200 BXD5 "75,548" "±1,273" "±2,846" 3.8% 6 "73,053" "78,043" "72,566" "79,857" "7,291" "±2,880" 2.31 63 18.9 514 48 0.969 1.872 "75,548" 49 0.990 0.080 2.319 514 " 8,101,344 " 15.9 5 80 0.200 BXD6 "62,695" ±801 "±1,961" 3.1% 7 "61,126" "64,264" "59,911" "64,712" "4,801" "±1,776" 0.23 163 19.8 392 25 0.500 0.000 "62,695" 5 0.092 0.159 -1.330 392 " 3,844,884 " 15.2 6 91 0.167 BXD8 "60,153" "±1,433" "±2,026" 3.4% 3 "57,344" "62,961" "57,856" "61,691" "3,834" "±1,419" -0.19 259 21.1 430 19 0.378 -0.312 "60,153" 26 0.520 #DIV/0! 0.051 430 " 4,106,221 " 15.2 2 30 0.500 BXD9 "66,053" "±1,611" "±3,602" 5.5% 6 "62,896" "69,210" "61,325" "69,342" "8,017" "±3,167" 0.77 185 22.8 439 39 0.786 0.792 "66,053" 30 0.602 -0.984 0.259 439 " 12,973,224 " 16.4 5 82 0.200 BXD11 "60,819" "±1,451" "±4,104" 6.7% 9 "57,975" "63,663" "56,124" "66,474" "10,351" "±3,488" -0.08 66 19.7 430 20 0.398 -0.259 "60,819" 24 0.480 0.418 -0.051 430 " 16,839,909 " 16.6 8 133 0.125 BXD12 "54,788" ±943 "±1,886" 3.4% 5 "52,940" "56,636" "53,045" "57,857" "4,813" "±2,069" -1.06 37 18.1 408 13 0.255 -0.659 "54,788" 14 0.276 -0.596 408 " 3,555,164 " 15.1 4 60 0.250 BXD13 "54,101" "±2,086" "±4,171" 7.7% 5 "50,014" "58,189" "56,328" "58,053" "1,725" "±1,019" -1.17 27 18.6 402 7 0.133 -1.114 "54,101" 10 0.194 0.204 -0.864 402 " 17,397,856 " 16.7 4 67 0.250 BXD14 "65,283" "±1,628" "±3,987" 6.1% 7 "62,093" "68,473" "59,564" "70,267" "10,704" "±3,960" 0.64 68 21.8 436 37 0.745 0.659 "65,283" 29 0.582 0.824 0.206 436 " 15,896,278 " 16.6 6 99 0.167 BXD15 "64,235" ±989 "±2,211" 3.4% 6 "62,298" "66,173" "61,004" "67,213" "6,208" "±2,669" 0.47 100 23.4 442 32 0.643 0.366 "64,235" 33 0.663 -0.265 0.421 442 " 4,887,563 " 15.4 5 77 0.200 BXD16 "64,255" "±2,517" "±6,166" 9.6% 7 "58,303" "70,208" "55,934" "75,614" "19,680" "±7,773" 0.48 64 21 465 35 0.704 0.536 "64,255" 43 0.867 0.211 1.114 465 " 38,021,869 " 17.5 6 105 0.167 BXD18 "54,266" "±1,147" "±2,565" 4.7% 6 "52,018" "56,514" "50,627" "58,177" "7,551" "±2,983" -1.14 113 20.3 413 9 0.173 -0.941 "54,266" 17 0.337 0.053 -0.421 413 " 6,577,362 " 15.7 5 78 0.200 BXD19 "67,558" "±1,581" "±4,337" 6.4% 9 "64,458" "70,657" "61,653" "74,741" "13,089" "±4,411" 1.01 108 19.5 423 44 0.888 1.215 "67,558" 21 0.418 0.023 -0.206 423 " 18,809,642 " 16.7 8 134 0.125 BXD20 "62,184" "±1,382" "±3,909" 6.3% 9 "59,475" "64,892" "55,879" "68,289" "12,410" "±4,182" 0.14 71 17.7 380 23 0.459 -0.102 "62,184" 4 0.071 0.325 -1.465 380 " 15,277,059 " 16.5 8 132 0.125 BXD21 "59,968" "±1,422" "±3,763" 6.3% 8 "57,180" "62,756" "54,618" "64,885" "10,267" "±3,799" -0.22 114 24.8 449 18 0.357 -0.366 "59,968" 36 0.724 0.025 0.596 449 " 14,160,186 " 16.5 7 115 0.143 BXD22 "64,802" "±1,869" "±3,738" 5.8% 5 "61,138" "68,466" "60,994" "70,785" "9,790" "±3,622" 0.57 38 17 430 36 0.724 0.596 "64,802" 25 0.500 -0.086 0.000 430 " 13,975,969 " 16.5 4 66 0.250 BXD23 "54,535" "±1,981" "±4,429" 8.1% 6 "50,653" "58,417" "50,750" "60,873" "10,123" "±3,999" -1.10 86 19.6 421 11 0.214 -0.792 "54,535" 20 0.398 0.009 -0.259 421 " 19,614,654 " 16.8 5 84 0.200 BXD24 "62,395" ±992 "±2,429" 3.9% 7 "60,451" "64,338" "58,714" "65,631" "6,917" "±2,559" 0.18 267 23.5 413 24 0.480 -0.051 "62,395" 16 0.316 0.311 -0.478 413 " 5,898,667 " 15.6 6 94 0.167 BXD25 "54,759" "±1,418" "±3,171" 5.8% 6 "51,979" "57,539" "50,469" "58,753" "8,283" "±3,562" -1.06 60 17.8 409 12 0.235 -0.723 "54,759" 15 0.296 0.262 -0.536 409 " 10,058,151 " 16.1 5 81 0.200 BXD27 "51,568" "±1,391" "±3,110" 6.0% 6 "48,842" "54,294" "48,194" "55,662" "7,468" "±3,211" -1.58 38 15.3 369 2 0.031 -1.872 "51,568" 2 0.031 0.995 -1.872 369 " 9,672,541 " 16.1 5 80 0.200 BXD28 "53,864" "±3,624" "±7,249" 13.5% 5 "46,761" "60,968" "44,367" "60,534" "16,167" "±7,857" -1.21 56 18.9 405 6 0.112 -1.215 "53,864" 12 0.235 -0.031 -0.723 405 " 52,542,002 " 17.8 4 71 0.250 BXD29 "63,539" "±1,859" "±4,156" 6.5% 6 "59,896" "67,182" "58,132" "69,862" "11,729" "±4,633" 0.36 65 19.7 398 29 0.582 0.206 "63,539" 7 0.133 0.508 -1.114 398 " 17,273,269 " 16.7 5 83 0.200 BXD30 "68,079" "±2,154" "±4,816" 7.1% 6 "63,858" "72,300" "58,132" "66,877" "8,745" "±4,250" 1.10 55 18.4 377 45 0.908 1.330 "68,079" 3 0.051 0.133 -1.635 377 " 23,192,379 " 17.0 5 85 0.200 BXD31 "66,833" "±2,038" "±4,556" 6.8% 6 "62,840" "70,827" "59,184" "71,842" "12,659" "±5,000" 0.90 51 21.3 415 42 0.847 1.023 "66,833" 18 0.357 -0.130 -0.366 415 " 20,757,645 " 16.8 5 84 0.200 BXD32 "76,836" "±3,355" "±7,502" 9.8% 6 "70,260" "83,412" "67,181" "85,632" "18,451" "±7,934" 2.52 47 19.6 429 49 0.990 2.319 "76,836" 23 0.459 0.817 -0.102 429 " 56,283,124 " 17.8 5 89 0.200 BXH4 "68,666" "±2,282" "±3,227" 4.7% 3 "64,194" "73,139" "49,213" "72,115" "22,902" "±9,848" 1.19 60 21.2 479 46 0.929 1.465 "68,666" 47 0.949 0.753 1.635 479 " 10,415,034 " 16.2 2 32 0.500 BXH6 "51,926" "±1,627" "±2,818" 5.4% 4 "48,737" "55,114" "65,719" "55,689" "-10,030" "-±4,313" -1.52 68 18 450 3 0.051 -1.635 "51,926" 38 0.765 0.861 0.723 450 " 7,940,112 " 15.9 3 48 0.333 BXH9 "56,967" "±2,238" "±3,876" 0.0% 4 "52,581" "61,353" "53,323" "62,366" "9,043" "±3,888" -0.70 72 24 477 16 0.316 -0.478 "56,967" 45 0.908 0.080 1.330 477 " 15,022,705 " 16.5 3 50 0.333 BXH12 "69,674" ±791 "±1,369" 2.0% 4 "67,479" "71,869" "68,179" "71,498" "3,319" "±1,613" 1.36 58 19 432 47 0.949 1.635 "69,674" 28 0.561 0.753 0.154 432 " 1,875,437 " 14.4 3 43 0.333 BXH14 "66,023" "±3,297" "±7,372" 11.2% 6 "57,956" "74,091" "54,231" "72,980" "18,749" "±8,062" 0.76 51 19 421 38 0.765 0.723 "66,023" 19 0.378 0.861 -0.312 421 " 54,350,801 " 17.8 5 89 0.200 BXH19 "54,136" "±2,660" "±4,608" 8.5% 4 "46,749" "61,522" "48,761" "59,761" "11,000" "±5,346" -1.16 44 18 432 8 0.153 -1.023 "54,136" 27 0.541 0.080 0.102 432 " 21,232,982 " 16.9 3 51 0.333 C3H/HeJ "67,039" "±1,625" "±3,634" 5.4% 6 "63,854" "70,224" "60,818" "71,259" "10,441" "±4,124" 0.93 85 29.8 510 14.1 H inbred c/c-rd/rd done 43 0.867 1.114 "67,039" 48 0.969 1.872 510 " 13,204,312 " 16.4 5 82 0.200 C57BL/6JAx1 "66,082" "±1,605" "±4,540" 6.9% 9 "62,451" "69,713" "58,641" "71,770" "13,129" "±4,424" 1.16 78 19.5 459 41 0.827 0.941 "66,082" 40 0.806 0.581 0.864 459 " 20,611,798 " 16.8 8 135 0.125 C57BL/6J "54,320" "±1,347" "±5,216" 9.6% 16 "51,680" "56,960" "47,070" "65,653" "18,582" "±6,039" -1.13 79 19.5 465 14.7 "16,895" ±256 3.2 L inbred c/c and +/+ 3 in prog 10 0.194 -0.864 "54,320" 42 0.847 0.298 1.023 465 " 27,205,954 " 17.1 15 257 0.067 C57BLKS/J "64,238" "±2,572" "±6,301" 9.8% 7 "59,196" "69,279" "9,319" "82,621" "73,302" "±23,823" 0.48 51 18.3 442 - "14,595" ±900 4.4 H inbred "2 males in prog, 1 replacement feamle ordered Feb 95" 33 0.663 0.421 "64,238" 32 0.643 0.844 0.366 442 " 39,698,606 " 17.5 6 105 0.167 CBA/CaJ "56,028" "±1,145" "±2,561" 4.6% 6 "53,784" "58,273" "52,809" "60,281" "7,472" "±2,951" -0.86 45 16.6 440 - ? inbred 2 in prog 15 0.296 -0.536 "56,028" 31 0.622 0.312 440 " 6,556,583 " 15.7 5 78 0.200 CE/J "63,593" "±2,384" "±4,767" 7.5% 5 "58,921" "68,265" "55,233" "66,888" "11,655" "±5,012" 0.37 96 23.9 462 - H inbred 1 in prog 30 0.602 0.259 "63,593" 41 0.827 -0.030 0.941 462 " 22,728,117 " 16.9 4 68 0.250 DBA/2J "63,351" "±1,137" "±4,100" 6.5% 13 "61,122" "65,579" "57,625" "69,775" "12,150" "±3,730" 0.33 55 16.9 401 - "15,435" ±320 4.1 H inbred 6 in progress 27 0.541 0.102 "63,351" 9 0.173 -0.047 -0.941 401 " 16,807,933 " 16.6 12 200 0.083 LP/J "52,225" "±1,919" "±5,078" 9.7% 8 "48,463" "55,987" "46,158" "59,057" "12,899" "±4,528" -1.47 108 21.7 365 - L inbred A/A done 4 0.071 -1.465 "52,225" 1 0.010 0.532 -2.319 365 " 25,787,354 " 17.1 7 119 0.143 NZB/BinJ "61,135" "±1,226" "±2,742" 4.5% 6 "58,731" "63,538" "56,090" "63,967" "7,878" "±3,112" -0.03 118 27.5 471 - ? inbred 3 in prog 21 0.418 -0.206 "61,135" 44 0.888 1.215 471 " 7,518,005 " 15.8 5 79 0.200 NZW/LacJ "64,253" "±1,444" "±2,501" 3.9% 4 "61,423" "67,083" "62,117" "67,765" "5,648" "±2,745" 0.48 159 31.8 459 - H inbred c/c 1 in prog 34 0.684 0.478 "64,253" 39 0.786 0.792 459 " 6,252,909 " 15.6 3 47 0.333 PL/J "55,976" "±1,263" "±3,341" 6.0% 8 "53,501" "58,451" "50,498" "60,287" "9,789" "±3,436" -0.86 43 18.3 446 - L inbred c/c-rd/rd done 14 0.276 -0.596 "55,976" 34 0.684 0.478 446 " 11,162,525 " 16.2 7 114 0.143 SJL/J "52,473" "±1,684" "±3,766" 7.2% 6 "49,172" "55,775" "51,788" "56,439" "4,651" "±1,837" -1.43 42 15.8 400 L inbred rd/rd 3 in prog 5 0.092 -1.330 "52,473" 8 0.153 -0.842 -1.023 400 " 14,186,265 " 16.5 5 82 0.200 Average "61,307" "±1,711" "±3,905" ±0.36% 6.59 6.4% "11,153" "±4,266" 82 20.4 431 correlations: 0.975 correlations: 0.993 Bartlett's M= 100.90 804.1 274 Strain SD and SEM "±6,168" ±645 "±1,416" 2.5% Correl with RGC 0.056 0.256 0.386 0.14863 0.267 C= 1.087 Count 49 49 49 6.27% 323 Correl with brain 0.047 0.505 — 2.520 M/C 92.844 with df of a-1 48 0.0196 0.7218951 ±2.819= corrected average SD 0.1196 is correlation between CV and RGC number r crit 5% with 20 df is 0.423 49 See Snedecor and Cochran (1980) p 252. ±7.02 4.5% 0.211111111 2.6% 0.720 0.830 0.078 Standard Inbred Only "t dist, 2-tailed, p=0.025 per tail)" 0.949 Strain Neurons SEM SD CV N 95%CI- 95%CI+ Lowest Highest Range SD_Range Z High_Low Age Body Brain BrainSE BrainSD BrainCV BrainN Type Note Rank_RGC Z_Frac_RGC Z_exp_RGC Rank_Brain Z_Frac_Brain Z_exp_Brain Brain Z_exp RGC Females %Females 129/J "63,772" "±1,699" "±4,163" 6.5% 7 "59,754" "67,791" "57,185" "68,409" "11,223" "±4,153" 0.72 H 76 20.0 407 ±5.2 ±15 3.8% 10 c/c done 13 0.735 0.629 6 0.324 -0.458 407 0.629 "63,772" 3 43% A/J "51,238" "±1,362" "±3,602" 7.0% 8 "48,098" "54,377" "46,312" "56,720" "10,408" "±3,653" -1.65 L 111 22.2 395 ±12.3 ±30 7.7% 7 A/A done. Ordered 1 male and 1 female for F1 production. High incidence of cleft palate. 1 0.029 -1.890 3 0.147 -1.049 395 -1.890 "51,238" 3 38% AKR/J "62,788" ±890 "±1,990" 3.2% 6 "60,611" "64,966" "60,063" "65,143" "5,081" "±2,007" 0.53 H 71 NA 461 ±12.4 ±28 6.0% 6 c/c done. A short-lived strain 9 0.500 0.000 12 0.676 0.458 461 0.000 "62,788" 5 83% BALB/cBy "57,558" "±1,768" "±4,330" 7.5% 7 "53,379" "61,738" "51,320" "63,924" "12,604" "±4,663" -0.46 ? 30 14.4 403 ±11.9 ±31 7.8% 8 c/c done. Males are not aggressive. Separated by 100 generations from BALB/cJ. Ok breeders. 7 0.382 -0.299 5 0.265 -0.629 403 -0.299 "57,558" 5 71% BALB/cJ "63,393" "±2,210" "±5,846" 9.2% 8 "58,298" "68,488" "53,252" "68,788" "15,536" "±5,453" 0.65 H 96 23.9 477 ±9.0 ±22 4.6% 7 c/c 3 in prog. Males are highly aggressive. Poor breeders. 11 0.618 0.299 16 0.912 1.352 477 0.299 "63,393" 4 50% C3H/HeJ "67,039" "±1,625" "±3,634" 5.4% 6 "63,063" "71,015" "60,818" "71,259" "10,441" "±4,124" 1.34 H 85 29.8 510 ±4.2 ±6 1.2% 3 c/c-rd/rd done 17 0.971 1.890 17 0.971 1.890 510 1.890 "67,039" 5 83% C57BL/6JAx1 "66,082" "±1,605" "±4,540" 6.9% 9 "62,451" "69,713" "58,641" "71,770" "13,129" "±4,858" 1.16 H 78 19.5 459 ±5.3 ±17 3.6% 11 +/+ These cases all from Annex 1. Ordered 6 more not from Annex 1. 16 0.912 1.352 11 0.618 0.299 459 1.352 "66,082" 4 44% C57BL/6J "54,320" "±1,347" "±5,216" 9.6% 16 "51,465" "57,175" "47,070" "65,653" "18,582" "±6,039" -1.07 L 79 21.5 472 ±4.5 ±25 5.2% 31 "+/+, c/c" Original set prior to 1994. Includes pigemented and coisogenic albinos 4 0.206 -0.821 15 0.853 1.049 472 -0.821 "54,320" 4 25% C57BLKS/J "64,238" "±2,572" "±6,301" 9.8% 7 "58,155" "70,320" "9,319" "82,621" "73,302" "±23,823" 0.81 ? 114 21.0 445 ±4.6 ±14 3.1% 10 3 in prog 14 0.794 0.821 8 0.441 -0.148 445 0.821 "64,238" 10 143% CBA/CaJ "56,028" "±1,145" "±2,561" 4.6% 6 "53,226" "58,831" "52,809" "60,281" "7,472" "±2,951" -0.75 L 45 16.6 440 ±16.3 ±16 3.7% 2 no rd done 6 0.324 -0.458 7 0.382 -0.299 440 -0.458 "56,028" 3 50% CE/J "63,593" "±2,384" "±4,767" 7.5% 5 "57,465" "69,720" "55,233" "66,888" "11,655" "±5,012" 0.69 H 96 23.9 462 ±7.1 ±16 3.4% 6 c^e/c^e. Dirty white coat. done 12 0.676 0.458 13 0.735 0.629 462 0.458 "63,593" 1 20% DBA/2J "63,351" "±1,137" "±4,100" 6.5% 13 "60,894" "65,807" "57,625" "69,775" "12,150" "±3,730" 0.64 H 55 16.9 394 ±7.6 ±23 5.8% 10 3 in prog 10 0.559 0.148 2 0.088 -1.352 394 0.148 "63,351" 6 46% LP/J "52,225" "±1,919" "±5,078" 9.7% 8 "47,799" "56,651" "46,158" "59,057" "12,899" "±4,528" -1.47 L 108 21.7 365 ±8.3 ±18 5.1% 6 A/A done 2 0.088 -1.352 1 0.029 -1.890 365 -1.352 "52,225" 4 50% NZB/BinJ "61,135" "±1,226" "±2,742" 4.5% 6 "58,134" "64,135" "56,090" "63,967" "7,878" "±3,112" 0.22 H 118 27.5 471 ±7.9 ±11 2.4% 3 done 8 0.441 -0.148 14 0.794 0.821 471 -0.148 "61,135" 3 50% NZW/LacJ "64,253" "±1,444" "±2,501" 3.9% 4 "60,245" "68,261" "62,117" "67,765" "5,648" "±2,745" 0.81 H 159 31.8 459 — — — — c/c done 15 0.853 1.049 10 0.559 0.148 459 1.049 "64,253" 2 50% PL/J "55,976" "±1,263" "±3,341" 6.0% 8 "53,064" "58,888" "50,498" "60,287" "9,789" "±3,436" -0.76 L 43 18.3 446 ±14.1 ±20 4.5% 3 c/c-rd/rd done 5 0.265 -0.629 9 0.500 0.000 446 -0.629 "55,976" 2 25% SJL/J "52,473" "±1,684" "±3,766" 7.2% 6 "48,352" "56,595" "45,570" "56,439" "10,869" "±4,293" -1.42 L 42 15.8 400 ±9.4 ±19 4.7% 5 rd/rd done 3 0.147 -1.049 4 0.206 -0.821 400 -1.049 "52,473" 4 67% Average "59,968" "±1,605" "±4,028" 6.76% 7.65 "14,627" "±5,211" 83 21.5 439 ±19 4.5% 8.00 0.94851838 Correlation: 0.972 0.949 68 55% Strain SD and SEM "±5,277" ±461 "±1,201" 0.51% " 1,226.12 " Correl with RGC 0.260 0.440 0.583 0.340 128 Count 17 ±300 2.1% 130 Correl with brain 0.230 0.598 —- F (Sept 95) 11.4 with 16 and 113 df P=6E-17 3.6% r crit 5% with 15 df is 0.482 F1 isogenic hybrids "t dist, 2-tailed, p=0.025 per tail)" Strain Neurons SEM SD CV N 95%CI- 95%CI+ Lowest Highest Range SD_Range Mat_Strain Pat_Strain MidParent Diff [d] [h] Potency_ratio High_Low Age Body Brain BrainSD BrainCV Action Type (FxM) RGC-Brain Correl B6AxCF1/JGo "64,854" "±2,408" "±5,898" 9.1% 7 "59,160" "70,547" "56,718" "74,205" "17,487" "±6,470" "66,082" "63,393" "64,738" 116 "1,345" 116 0.09 I 65 21.6 478 ±26 additive C57BL/6JAx1 by BALB/cJ 0.586 CB6AxF1/J (?) "66,688" "±1,094" "±2,446" 3.7% 6 "64,011" "69,365" "63,427" "70,088" "6,661" "±2,631" "63,393" "66,082" "64,738" "1,950" "1,345" "1,950" 1.45 H 50 22.8 517 — over-dominance BALB/c by C57BL/6J n=2 CAF1/J "56,147" "±1,794" "±4,010" 7.1% 6 "51,758" "60,535" "51,815" "62,614" "10,799" "±4,266" "63,393" "51,238" "57,315" "-1,169" "6,078" "1,169" 0.19 I 36 20.0 468 ±10 additive BALB/c by A/J 0.694 CCASF1 "59,705" "±1,278" "±3,615" 6.1% 9 "56,814" "62,597" "55,238" "64,436" "9,198" "±3,100" "63,393" "45,484" "54,439" "5,267" "8,954" "5,267" 0.59 I 121 25.8 492 ±27 weak dominance BALB/c by CAST/Ei 0.303 PLSJF1/J "56,096" "±1,279" "±3,134" 5.6% 7 "53,071" "59,120" "50,030" "58,973" "8,944" "±3,309" "55,976" "52,473" "54,225" "1,871" "1,751" "1,871" 1.07 I 54 21.0 438 ±12 additive PL/J by SJL/J -0.001 Average "60,698" "±1,571" "±3,821" 6.31% 7.00 "10,618" "±3,955" "1,607" "3,894" 65 22.2 478 19 Strains SD "±4,900" ±536 1.00% Correl with RGC 0.057 0.296 0.783 predicted average "±59,091" excess= "1,607" 2.0% 3.09% Correl with brain 0.550 Count 5 corrected 2.71% 35 r crit 5% with 3 df is 0.878 See BXD Residuals Spreadsheet "B <62,000)" "t dist, 2-tailed, p=0.025 per tail)" <425 = D type "Proximal Chr 11: D11Mit2, D11Byu1" "Belknap JK, Phillips TJ, O'Toole LA (1992) Quantitative trait loci associaed with brain weight in the BXD/Ty recombinant inbred mouse strains. Brain Res Bull 29:337-344. 8 males per strain" BXD RI Strains Neurons SEM SD CV N RGC_Class1* RGC_Class2** Tstap91A Z 95%CI- 95%CI+ Lowest Highest Range SD_Range Age Body Brain BrainSE BrainSD BrainCV BrainN Brain_Class D11Ncvs74 Belknap_BXD ±SD Note Rank_RGC Z_Fract Z_Exp RGCs Rank_Brain Z_Fract_Brain Z_Exp_Brain Brain Cerebellum "Redone Jan 9, 96" C57BL/6J "54,320" "±1,347" "±5,216" 9.6% 16 B B B -1.00 "51,465" "57,175" "47,070" "65,653" "18,582" "±5,853" 79 21.5 472 ±5.3 ±29 6.1% 31 B B 465 ±23 5 0.161 -0.992 "54,320" 27 0.855 1.059 472 From BW For QTL mapping Reference for r 2 grade Strain Actual Predicted Residuals Tstap91A Residuals BXD1 "61,996" "±1,077" "±3,231" 5.2% 10 U U B 0.70 "59,596" "64,396" "59,552" "67,167" "7,615" "±2,673" 51 20.5 448 ±6.9 ±23 5.1% 12 B B 450 ±22 "done, 4 more received from JAX via Dan, July 95" 12 0.411 -0.226 "61,996" 24 0.758 0.701 448 BXD1 61425 " 64,851 " "-3,426" 0 0 0 BXD2 "66,063" "±1,644" "±3,677" 5.6% 6 D D D 1.61 "62,039" "70,087" "60,417" "70,774" "10,358" "±4,091" 53 23.1 427 ±8.2 ±18 4.3% 6 B B 390 ±19 done 23 0.804 0.854 "66,063" 16 0.500 0.000 427 BXD2 66063 " 62,575 " "3,488" 1 1 1 BXD5 "75,548" "±1,273" "±2,846" 3.8% 6 D D D 3.72 "72,433" "78,662" "72,566" "79,857" "7,291" "±2,880" 63 18.9 514 ±8.7 ±23 4.5% 8 B B 477 ±27 "done, 3 breeders in colony Aug 95" 27 0.946 1.611 "75,548" 28 0.887 1.213 514 51 BXD5 75548 " 71,826 " "3,721" 1 1 1 BXD6 "62,695" ±801 "±1,961" 3.1% 7 D D D 0.86 "60,802" "64,588" "59,911" "64,712" "4,801" "±1,776" 163 19.8 392 ±3.6 ±9 2.4% 8 D D 361 ±16 done 15 0.518 0.045 "62,695" 4 0.113 -1.211 392 43 BXD6 62695 " 58,949 " "3,746" 1 1 1 BXD8 "60,153" "±1,433" "±2,026" 3.4% 3 B U D 0.29 "55,592" "64,713" "57,856" "61,691" "3,834" "±1,419" 259 21.1 430 ±3.6 ±11 2.5% 10 B B 421 ±13 done. Could use 3 young mice. I suspect the low average. 10 0.339 -0.414 "60,153" 20 0.629 0.330 430 BXD8 56905 " 62,943 " "-6,038" 1 0 0 BXD9 "66,053" "±1,611" "±3,602" 5.5% 6 D D D 1.60 "62,112" "69,995" "61,325" "69,342" "8,017" "±3,167" 185 22.8 439 ±11.3 ±16 3.6% 3 B B 419 ±15 done 22 0.768 0.732 "66,053" 22 0.694 0.507 439 BXD9 65732 " 63,917 " "1,815" 1 1 1 BXD11 "60,819" "±1,451" "±4,104" 6.7% 9 B U B 0.44 "57,537" "64,101" "56,124" "66,474" "10,351" "±4,089" 66 19.7 430 ±4.0 ±12 2.8% 10 B B NA NA "4 more received from Jax by Dan, July 95" 11 0.375 -0.319 "60,819" 18 0.565 0.163 430 BXD11 60757 " 62,894 " "-2,138" 0 0 0 BXD12 "54,788" ±943 "±1,886" 3.4% 5 B B B -0.90 "52,365" "57,211" "53,045" "57,857" "4,813" "±2,069" 37 18.1 408 ±6.4 ±6 1.6% 2 D B 412 ±13 done 8 0.268 -0.619 "54,788" 9 0.274 -0.600 408 BXD12 53553 " 60,633 " "-7,080" 0 0 0 BXD13 "54,101" "±2,086" "±4,171" 7.7% 5 B B B -1.05 "48,740" "59,462" "56,328" "58,053" "1,725" "±1,019" 27 18.6 402 ±4.5 ±9 2.2% 5 D D 403 ±9 "First three all males. 1 more received by Dan at Jax, July 95" 3 0.089 -1.345 "54,101" 7 0.210 -0.807 402 BXD13 54665 " 59,988 " "-5,322" 0 0 0 BXD14 "65,283" "±1,628" "±3,987" 6.1% 7 D D D 1.43 "61,434" "69,132" "59,564" "70,267" "10,704" "±3,960" 68 21.8 436 ±6.0 ±15 3.4% 7 B D 439 ±9 done 21 0.732 0.619 "65,283" 21 0.662 0.417 436 BXD14 65996 " 63,523 " "2,473" 1 1 1 BXD15 "64,235" ±989 "±2,211" 3.4% 6 D U D 1.20 "61,816" "66,655" "61,004" "67,213" "6,208" "±2,452" 100 23.4 442 ±9.4 ±21 4.8% 6 B B 429 ±14 Need 4 more because of low residuals after brain weight regression 18 0.625 0.319 "64,235" 23 0.726 0.601 442 BXD15 63765 " 64,224 " -459 1 weak 0 BXD16 "64,255" "±2,517" "±6,166" 9.6% 7 D U D 1.20 "58,303" "70,208" "55,934" "75,614" "19,680" "±7,773" 64 21.4 465 ±7.4 ±20 4.2% 8 B B 436 ±16 "3 received May 95, additional 4 from Jax by Dan, July 95" 19 0.661 0.414 "64,255" 26 0.823 0.926 465 48 BXD16 65109 " 66,425 " "-1,315" 1 weak 0 BXD18 "54,266" "±1,147" "±2,565" 4.7% 6 B B B -1.02 "51,459" "57,072" "50,627" "58,177" "7,551" "±2,983" 113 20.3 413 ±8.0 ±14 3.3% 4 D D 427 ±13 1 in prog 4 0.125 -1.150 "54,266" 12 0.371 -0.329 413 BXD18 55743 " 61,183 " "-5,440" 0 0 0 BXD19 "67,558" "±1,581" "±4,337" 6.4% 9 D D D 1.94 "63,981" "71,135" "61,653" "74,741" "13,089" "±4,411" 108 19.5 423 ±6.7 ±19 4.5% 9 B B NA NA done 25 0.875 1.150 "67,558" 15 0.468 -0.081 423 BXD19 67624 " 62,238 " "5,386" 1 1 1 BXD20 "62,184" "±1,382" "±3,909" 6.3% 9 U U D 0.74 "59,058" "65,310" "55,879" "68,289" "12,410" "±4,356" 71 17.7 380 ±3.9 ±13 3.4% 12 D D NA NA 1 in prog 13 0.446 -0.135 "62,184" 3 0.081 -1.401 380 41 BXD20 61915 " 57,624 " "4,291" 1 1 1 BXD21 "59,968" "±1,422" "±3,763" 6.3% 8 B U D 0.25 "56,688" "63,248" "54,618" "64,885" "10,267" "±3,799" 114 24.8 449 ±7.3 ±19 4.3% 8 B B 409 ±20 done 9 0.304 -0.514 "59,968" 25 0.791 0.809 449 45 BXD21 59236 " 64,964 " "-5,728" 1 0 0 BXD22 "64,802" "±1,869" "±3,738" 5.8% 5 D D D 1.33 "59,997" "69,607" "60,994" "70,785" "9,790" "±4,210" 38 17.3 430 ±5.4 ±11 2.5% 5 B B 431 ±10 3 in progress 20 0.696 0.514 "64,802" 19 0.597 0.246 430 BXD22 64802 " 62,908 " "1,894" 1 1 1 BXD23 "54,535" "±1,981" "±4,429" 8.1% 6 B B B -0.96 "49,689" "59,381" "50,750" "60,873" "10,123" "±3,999" 86 19.6 421 ±8.0 ±18 4.3% 6 D B 432 ±30 1 in prog. 2 backorders canceled May 95 6 0.196 -0.854 "54,535" 14 0.436 -0.162 421 BXD23 54535 " 62,005 " "-7,470" 0 0 0 BXD24 "62,395" ±992 "±2,429" 3.9% 7 D U D 0.79 "60,050" "64,739" "58,714" "65,631" "6,917" "±2,559" 267 23.5 413 ±6.9 ±18 4.4% 8 D D 380 ±17 done 14 0.482 -0.045 "62,395" 11 0.339 -0.416 413 BXD24 62254 " 61,108 " "1,146" 1 weak 1 BXD25 "54,759" "±1,418" "±3,171" 5.8% 6 B B B -0.91 "51,288" "58,229" "53,110" "58,753" "5,643" "±2,229" 60 17.8 409 ±5.5 ±13 3.3% 7 D B 394 ±16 1 back-ordered? Cut other side of case H 7 0.232 -0.732 "54,759" 10 0.307 -0.506 409 BXD25 55768 " 60,742 " "-4,974" 0 0 0 BXD27 "51,568" "±1,391" "±3,110" 6.0% 6 B B B -1.62 "48,165" "54,971" "48,194" "55,662" "7,468" "±2,950" 41 15.5 365 ±6.9 ±15 4.2% 6 D D 381 ±18 done: One case had hydrocephaly. 1 0.018 -2.100 "51,568" 1 0.016 -2.141 365 42 BXD27 51568 " 56,055 " "-4,487" 0 0 0 BXD28 "53,864" "±3,624" "±7,249" 13.5% 5 B B B -1.11 "44,548" "63,181" "44,367" "60,534" "16,167" "±7,857" 56 18.9 405 ±7.7 ±17 4.3% 6 D D 389 ±20 2 in colony 2 0.054 -1.611 "53,864" 8 0.242 -0.700 405 BXD28 51114 " 60,323 " "-9,209" 0 0 0 BXD29 "63,539" "±1,859" "±4,156" 6.5% 6 D U D 1.05 "58,991" "68,087" "58,132" "69,862" "11,729" "±4,633" 65 19.7 398 ±8.0 ±18 4.5% 6 D D 375 ±11 done 17 0.589 0.226 "63,539" 6 0.177 -0.925 398 BXD29 63539 " 59,513 " "4,026" 1 1 1 BXD30 "68,079" "±2,154" "±4,816" 7.1% 6 D D D 2.06 "62,809" "73,349" "61,398" "72,960" "11,562" "±4,971" 55 18.4 377 ±5.6 ±14 3.6% 7 D D NA NA "4 sent by Dan from JAX, July 95" 26 0.911 1.345 "68,079" 2 0.048 -1.661 377 BXD30 68079 " 57,336 " "10,743" 1 1 1 BXD31 "66,833" "±2,038" "±4,556" 6.8% 6 D D B 1.78 "61,848" "71,819" "59,184" "71,842" "12,659" "±5,000" 51 21.3 415 ±6.9 ±15 3.7% 6 D D NA NA done 24 0.839 0.992 "66,833" 13 0.403 -0.245 415 BXD31 65983 " 61,317 " "4,665" 0 1 1 BXD32 "76,836" "±3,355" "±7,502" 9.8% 6 D D D 4.00 "68,626" "85,046" "67,181" "85,632" "18,451" "±7,934" 47 19.6 429 ±2.6 ±11 2.5% 18 B D NA NA 4 in progress 28 0.982 2.100 "76,836" 17 0.532 0.081 429 BXD32 76836 " 62,872 " "13,964" 1 1 1 Correlations 0.670 0.264 0.618 0.740 0.632 DBA/2J "63,351" "±1,137" "±4,100" 6.5% 13 D D D 1.00 "60,894" "65,807" "57,625" "69,775" "12,150" "±3,730" 52 17.0 394 ±7.6 ±23 5.8% 10 D D 339 ±19 16 0.554 0.135 "63,351" 5 0.145 -1.057 394 critical with N of 25 =0.659 italic have weak residuals Average "61,959" "58,835" "±3,831" 6.17% 6.5 *Liberal **Stringent "9,585" "±3,818" 89 20.1 422 ±16 3.6% "F=14.77, p-.00083 with D11Ncvs74" 413 ±29 Belknap set average (no olfactory bulb) bold have discrepant residuals Count 28 26 766 0.46% 168 No alternative correl with RGC 0.017 0.259 0.489 0.24 426 ±29 Our equivalent set average (with bulb) SD brain ±31.0 explain 45% SD across BXD "±4,498" 2.37% "Tstap91A (at 45 cM, 2 cM distal to Hoxb)" Jan 18 1996 QT Map Manager Links report correl with brain 0.060 0.444 — r= 0.82 Our BXD brains to Belknap's BXD brains Average D type "65,467" 15 5.1% F= "27.65 (with truncation of BXD5, and 32 to 68,000" Average B type "54,754" 8 5.5% p= 0.0000217 "2 in 100,000" r crit 5% with 20 df is 0.423 Average all BXDs "62,199" 23 5.3% 0.7185629 approx lod 4.60 if r=.443 then z = 0.475 F (Nov 95) of 18.7 with 25 and 156 df <450=B BXH RI Strains Neurons SEM SD CV N RGC_Class1* "Chr 2, Hdc" Chr 11 Mpmv4 (48 cM) Z 95%CI- 95%CI+ Lowest Highest Range SD_Range Age Body Brain BrainSE BrainSD BrainCV BrainN Brain_Class Cerebellum Belknap_Brain ±SD Note Rank_RGC Z_Fract Z_Exp RGCs Rank_Brain Z_Fract_Brain Z_Exp_Brain Brain C57BL/6J "54,320" "1,347" "5,216" 9.6% 16 B B -1.00 "51,465" "57,175" "47,070" "65,653" "18,582" "±5,853" 79 19.5 465 ±29 6.2% 465 ±23 6 0.196 -0.854 "54,320" #N/A #N/A #N/A 465 BXH2 "63,406" "4,397" "4,397" 6.9% 2 H B H "44,486" "82,327" 42 19 414 ±1 ±1 0.3% 2 B BXH3 "59,642" 1 U B H 47 20 461 ±15 ±15 3.3% 2 H BXH4 "68,666" "2,282" "3,227" 4.7% 3 H H H "61,404" "75,929" 60 21 479 ±14 ±25 5.2% 4 H BXH6 "51,926" "1,627" "2,818" 5.4% 4 B B H "47,409" "56,443" 68 18 450 ±8 ±15 3.2% 4 B BXH7 "60,779" 1 U B H 38 17 448 ±0 ±0 0.1% 2 B BXH8 "64,631" 1 H H H 27 12.0 395 ±1 ±1 0.2% 2 B BXH9 "56,967" "2,238" "3,876" 6.8% 4 B B B "50,754" "63,180" 72 24 477 ±3 ±5 1.1% 4 H BXH10 "64,931" 1 H H B 35 18 448 ±19 ±19 4.2% 2 B BXH11 "51,312" 1 B B B 54 20 422 1 B BXH12 "69,674" 791 "1,369" 2.0% 4 H H H "67,479" "71,869" "68,179" "71,498" "3,319" 58 19 432 ±3 ±4 1.0% 3 B BXH14 "66,023" "3,297" "7,372" 11.2% 6 H H H "57,956" "74,091" "54,231" "72,980" "18,749" 51 19 421 ±4 ±9 2.1% 6 B BXH19 "54,136" "2,660" "4,608" 8.5% 4 B B B "46,749" "61,522" "48,761" "59,761" "11,000" 44 18 432 ±13 ±22 5.0% 4 B C3H/HeJ "67,039" "±1,625" "±3,634" 5.4% 6 H H "63,063" "71,015" "59,061" "71,259" "12,197" "±3,842" 88 30 510 ±4 ±6 1.2% 3 H Midpoint "60,679" 32 Linkage stats of Jan 24 96: "best QTL match to Hdc on Chr 2 (p=0.001, F=20.9)" 440 ±8 ±30 2.5% No locus on Chr 11 with this pattern. Count 12 SD across BXH "6,290" Average H type "68,121" Average B type "54,138" Average all BXHs "61,008" "t dist, 2-tailed, p<0.025 per tail)" Outcrossed Strains Neurons SEM SD N CV H or L 95%CI- 95%CI+ Lowest Highest Range SD_Range Age Body Brain BrainSD BrainCV Brain_Neuron_Correl Brain_Neuron_Covariation Brain_Body_Corr CARL/RpGo "51,263" "±2,214" "±7,000" 11 13.7% I "46,391" "56,135" "39,546" "62,844" "23,298" "±7,339" 283 20.1 418 ±24 5.7% NA CCASF2/JGo "56,617" "±1,447" "±8,683" 75 15.3% I "53,734" "59,500" "40,595" "77,548" "36,954" "±9,904" 40 16.4 443 ±37 8.4% 0.038 2370 0.738 CD-1/Go "68,338" "±2,163" "±7,720" 14 11.3% H "63,699" "72,978" "57,243" "87,242" "29,998" "±8,820" 94 NA NA NA NA Average "58,739" "±7,801" 13.4% ±1.4% "±8,687" 7.1% SD "±8,733" 100 0.1225114 Count 3 0.12757351 "t dist, 2-tailed, p<0.025 per tail)" Wild Inbred Strains Neurons SEM SD N CV H or L 95%CI- 95%CI+ Lowest Highest Range SD_Range Z Type Age Body Brain BrainSD BrainCV Status RGC-Brain Correl CASA/Rk "48,917" "±2,958" "±5,123" 4 10.5% L "40,705" "57,128" "45,131" "56,171" "11,041" "±5,366" -0.65 Mus musculus castaneus 51 9.7 342 ±18 5.4% done 0.265 CAST/Ei "45,484" ±999 "±3,160" 11 6.9% L "43,285" "47,684" "41,341" "50,160" "8,819" "±2,778" -1.08 Mus musculus castaneus 153 13.4 373 ±31 8.4% done 0.427 CZECHII/Ei "59,207" "±3,341" "±8,183" 7 13.8% I "51,307" "67,106" "49,312" "73,647" "24,335" "±9,004" 0.66 Mus musculus musculus 116 12.6 350 ±16 4.5% done -0.125 MOLD/Rk "43,758" "±4,207" "±8,414" 5 19.2% L "32,943" "54,573" "32,348" "52,400" "20,052" "±9,745" -1.30 Mus musculus molossinus (rd/rd) 163 13.5 323 ±9 2.9% done (should get some young ones or other MOL strains) n=2 PANCEVO/Ei "64,300" "±1,316" "±2,943" 6 4.6% H "61,079" "67,520" "61,473" "67,279" "5,806" "±2,293" 1.30 Mus spicilegus (hortulanus) 145 15.3 436 ±12 2.8% done -0.730 SPRET/Ei "59,049" "±1,804" "±4,035" 6 6.8% I "54,634" "63,464" "53,323" "64,447" "11,124" "±4,394" 0.64 Mus spretus 33 10.2 336 ±25 7.6% done -0.034 WSB/Ei "57,380" "±1,698" "±4,492" 8 7.8% I "53,465" "61,295" "50,826" "63,021" "12,195" "±4,280" 0.43 Mus musclus domesticus 106 15.8 423 ±20 4.7% done 0.060 Average "54,014" "±5,193" 6.7 10.0% or 7.9±1.0 is CZECHII/Ei is excluded "±5,409" 369 ±19 5.2% Count 7 47 ±2.1% ±1.7% Strains SD "±7,888" 8.17% 7.11% when MOLD/Rk low case is excluded correl with RGC -0.255 0.231 0.564 0.32 F (Sept 95) =14.6 with 6 and 40 df and P=9E-9 correl with brain 0.294 0.769 — "t dist, 2-tailed, p<0.025 per tail)" Strain or Species RGCs SEM SD N CV H or L 95%CI- 95%CI+ Lowest Highest Range Type Note Age Body Brain BrainSD BrainCV N brain Correl RGC to brain 4.91161432 CAST/Ei = CAS "45,484" ±999 "±3,160" 11 6.9% L "43,285" "47,684" "41,341" "50,160" "8,819" Mus castaneus wild inbred strain 124 11.4 373 ±31 8.4% 4.0000 CCASF1/JGo "59,705" "±1,278" "±3,615" 9 6.1% I "56,814" "62,597" "55,238" "64,436" "9,198" BALB/c female by CAST/Ei male 161 24.3 489 ±27 5.5% CCASF2 "56,617" "±1,447" "±8,683" 75 15.3% I "53,734" "59,500" "33,515" "77,548" "44,033" BCF1 intercrossF2 progeny 40 15.6 428 ±34 7.9% 115 0.329 0.10838253 CCASCASN2/JGo "56,673" "±2,970" "±6,642" 6 11.7% I "49,406" "63,941" "50,317" "64,895" "14,578" BCF1 by C backcross progeny 39 15.2 417 ±30 7.2% CCASCN2 "60,679" "±1,571" "±3,847" 7 6.3% I "56,965" "64,393" "53,093" "63,409" "10,317" BCF1 by B backcross progeny As many as 40 cases embedded. Half brains sent to Wahlsten for corpus callosum analysis. 32 16.2 467 ±26 5.6% BALC/c = C "63,393" "±2,210" "±5,846" 8 9.2% H "58,298" "68,488" "53,252" "68,788" "15,536" BALB/c parent strain 96 23.9 477 ±22 4.6% count 6 116 midpoint "54,439" F2 male SE female SE r age body brain age 42 ±13 39 ±5 age body 16.5 ±5 15.1 ±3 body 0.743 brain 436 ±39 423 ±31 brain 0.487 0.738 RGC "58,044" "±9,927" "55,554" "±8,407" RGC 0.049 0.174 0.329 0.108383 RGC N 31 41 Brain N 45 64 Strain or Species RGCs SEM SD N CV H or L 95%CI- 95%CI+ Lowest Highest Range Type Note Age Body Brain BrainSD BrainCV N brain Correl RGC to brain CAST/Ei = CAS 153 13 373 31 8.4% 13 CAS32F1 75 18.7 458 25 5.5% 8 CAS32F2 37 15.7 424 26 6.2% 68 BXD32 119 24 435 19 4.3% 18 "t dist, 2-tailed, p<0.025 per tail)" Odd strains RGCs SEM SD N CV H or L 95%CI- 95%CI+ Type Body Brain TgN(Hbb-b1)83/LoGo "56,912" "±1,969" "±4,404" 6 7.7% I "52,093" "61,731" 100 NA NA B6C3Fe-a/a-rl/ "63,357" "±4,148" "±9,276" 6 14.6% I "53,206" "73,507" 106 NA NA Strain Status Sex Study Group Case_Mean_Axons Strain_Mean_Axons Strain_Axons_SE Strain_Axons_SD Strain_N Case_Mean_Axons Case_SEM Axons Sample_SEM Precision Nerve_Area Unmyelinated Std_Err_Unmyelinated Density Z_Score_RGC Z_Score_Brain RGCs JAX_Order_Type ID_number Side/level Strain_Brain_Weight DOB date_killed Age Body_weight Brain_weight Brain_weight_2 Cerebellum Retina_area Sex_Number Litter Note1 Note2 Necrosis DNA Mother Father Date_Photograhed Block Box_Slot Tissue_Score Hi_Mag Low_Mag Photos_By "Count_By, Checked_By" Area_of_Montage Negative_Area_Counted Sample_Area Coverage Photos_to_Cover_Nerve Samples Count_Sum Average_Axons_per_Photo Std_Dev_Axons_Photo Std_Err_Axons_Photo Photo 0 Photo 1 Photo 2 Photo 3 Photo 4 Photo 5 Photo 6 Photo 7 Photo 8 Photo 9 Photo 10 Photo 11 Photo 12 Photo 13 Photo 14 Photo 15 Photo 16 Photo 17 Photo 18 Photo 19 Photo 20 Photo 21 Photo 22 Photo 23 Photo 24 Photo 25 Photo 26 Photo 27 Photo 28 Photo 29 Photo 30 Photo 31 Photo 32 Photo 33 Photo 34 Photo 35 Photo 36 Photo 37 Photo 38 Photo 39 Photo 40 Photo 41 Photo 42 Photo 43 Photo 44 Photo 45 Photo 46 Photo 47 Photo 48 Photo 49 Photo 50 "set 2, 1" Criteria= 1 6 <50000 -0.2786 "=""=DR""" 1.1691 mean n 7.5 "59,467" "±3,687" "59,116" "±4,008" 6.92% =average of single estimates 310 23 571 #DIV/0! #DIV/0! #DIV/0! N mean SD SEM Precision harmonic 4.6 "±3,435" 5.70% =technical precision of single estimates 17 "rw=77 (58.8), rcs=96 (62.3), dr=44 (59.8), tk=129+24 (60.3 + 59.2), dt=18 (57.7)" 17 46428 3953 988 1.91% 0.5% "57,474" "1,766" final technical "±3,161" 5.24% =technical following replication "55,850" 2007.11905 58285.691 34570.3368 Conventions: Notes on status of data by row "1=in first studies, blank=not included in first studies, 2=0.00001, 3=0.00000001" "1=standard inbred, 2=BXD, 3=isogenic F1 hybrid, 4=wild, 5=mutants, 6=F2 progeny of F1 intercross, 8=backcross N1, 9=random bred or outcrossed" ID number is usually the date followed by a letter. Animals are perfused in A to Z sequence. Wrong equation "Average count for animal. If a nerve was recounted then this value is a variance weighted average (Bevington and Robinson 1992 Data Reduction and ErrorAnalysis, 2ed, p. 59). This value may average left and right nerves." "SEM of count. If a nerve was recounted then this value is a variance weighted SEM (Bevington and Robinson 1992 Data Reduction and ErrorAnalysis, 2ed, p. 59)." Estimated total population of myelinated and unmyelinated axons in the optic nerve. Standard error of the estimate based on the variation within the sample of micrographs. "Internal sample precision as a coefficient of variation. This value does not take into account external sources of inaccuracy, such as fixation, counting error, misidentification, etc." Nerve area in square microns. Estimate of the number of unmyelinated axons. If data are not shown they can be obtained from the raw counts in the micrographs columns. In these columns data for the two types are entered in the formula in this sequence = myelinated + unmyelinated. Standard error of the estimate of unmyelinated fibers based on the variation within the sample of micrographs. density of axons per 100 square microns Normal score for the ganglion cell population of a single case based upon the strain mean and standard deviation. Normal score for the brain weight of a single case based upon the strain mean and standard deviation. Direct estimate of ganglion cell number by Denny Rice from wholemounted HRP-reacted pairs of retinas. "In most cases right and left nerves are processed together. Therefore, the side of origin is usually lost." "date of birth in year, month, day format" "date killed in year, month, day format" "age in days, calculated or estimated" live body weight just prior to fixation "fixed brain weight measured within 2-7 days of fixation. Brains are blocked just behind the cerebellum. The caudal brain setem is discarded. Weighte almost always include the olfactory bulbs and usually the floculus, but not the pituitary." Data from Denny Rice based on processed HRP reacted retinal wholemounts. "1=Female, 0=Male" Arbitrary litter numbers used just to determine number of litters examined in each strain Incidence of necrotic fibers in the sample Notes on status of tissue taken for DNA extraction. This is work of Richelle Strom. Data that a sample was photographed. EM block number used to generate thin sections. There are usually two blocks per case from the right and left sides. Location of thin sections "4=great, 3=good, 2=ok, 1=poor. Poor cases have usually been discarded. However, they may have been retained in this database if brain weight data were available." The magnification of the images used to count axons. The magnification of the image used to measure the cross-sectional area of the optic nerve thin section. Initials of the person who sampled the nerve. "Initials of the person who first counted axons, followed by initials of the person who checked the count. Most cases were double or triple checked by Rob Williams." The corrected area of the cross-section of the thin section that was sampled. #DIV/0! " 5,877 " Strain Status Sex Study Group ID_Number Weighted_Strain_Mean Strain_Mean_Axons Strain_Axons_SE Strain_Axons_SD Strain_N Case_Mean_Axons Case_SEM Axons Sample_SEM Precision Nerve_Area Unmyelinated SEM_Unmyelinated Density Z_Score_RGC Z_Score_Brain RGCs JAX_Order_Type ID_number Side/level Strain_Brain_Weight DOB date_killed Age Body_weight Brain_weight Brain_weight_2 D11Mit1 Cerebellum Retina_area Sex_Number Litter Note1 Note2 Necrosis Tissue_DNA Mother Father Photo_Date Block Box_Slot Tissue_Score Hi_Mag Low_Mag Photos_By "Count_By, Checked_By" Area_of_Montage Negative_Area_Counted Sample_Area Coverage Photos_to_Cover_Nerve Samples Count_Sum Average_Axons_per_Photo Std_Dev_Axons_Photo Std_Err_Axons_Photo Weighting_A 1/Variance Even_Sample Odd_Sample %_diff Odd_vs_Even_Diff Photo 0 Photo 1 Photo 2 Photo 3 Photo 4 Photo 5 Photo 6 Photo 7 Photo 8 Photo 9 Photo 10 Photo 11 Photo 12 Photo 13 Photo 14 Photo 15 Photo 16 Photo 17 Photo 18 Photo 19 Photo 20 Photo 21 Photo 22 Photo 23 Photo 24 Photo 25 Photo 26 Photo 27 Photo 28 Photo 29 Photo 30 Photo 31 Photo 32 Photo 33 Photo 34 Photo 35 Photo 36 Photo 37 Photo 38 Photo 39 Photo 40 Photo 41 Photo 42 Photo 43 Photo 44 Photo 45 Photo 46 Photo 47 Photo 48 Photo 49 Photo 50 Photo 51 Photo 52 Photo 53 Photo 54 Photo 55 Photo 56 Photo 57 Photo 58 Photo 59 Photo 60 Photo 61 Photo 62 Photo 63 Photo 64 Photo 65 Photo 66 Photo 67 Photo 68 Photo 69 Photo 70 Photo 71 Photo 72 Photo 73 Photo 74 Photo 75 Photo 76 Photo 77 Photo 78 Photo 79 Photo 80 Photo 81 Photo 82 Photo 83 Photo 84 Photo 85 Photo 86 Photo 87 Photo 88 Photo 89 Photo 90 Photo 91 Photo 92 Photo 93 Photo 94 Photo 95 Photo 96 Photo 97 Photo 98 Photo 99 Photo 100 Photo 101 Photo 102 Photo 103 Photo 104 Photo 105 Photo 106 Photo 107 Photo 108 Photo 109 Photo 110 Photo 111 Photo 112 Photo 113 Photo 114 Photo 115 Photo 116 Photo 117 Photo 118 Photo 119 Photo 120 Photo 121 Photo 122 Photo 123 Photo 124 Photo 125 Photo 126 Photo 127 Photo 128 Photo 129 Photo 130 Photo 131 Photo 132 129/J F 1 1 121092C "62,384" "63,772" "±1,699" "±4,163" 7 "66,315" "±2,522" "66,315" "±2,522" 3.8% "60,663" "7,909" "1,156" 109.32 0.611 "61,344 " JR000690 R 407 921111 921211 30 16.5 NA 14.1 1 1 Albino. 15% unmyelinated. Low mag calibration taken from other case shot at same time. 1 necrotic on 0033 12-Jan-93 A4 "Box 21, Slot QÐ4" 4 "13,198 x" 202 x DR DR and RW "2,475 mm^2" "5,094 mm^2" 29.2 µm^2 1.40% 2075 29 927 32.0 ±6.43 ±1.22 1.0795E-02 1.6279E-07 71611 69036 96.4% " 2,575 " 39 30 33 41 37 32 40 35 28 38 32 32 38 29 23 31 35 24 21 34 15 29 26 32 41 29 37 40 26 129/J original F 1 1 062892C better "57,185" "±1,759" "57,292" "±2,120" 3.7% "91,289" 62.76 -1.582 "58,479 " JR000690 L 920629 200 26.0 440 18.1 1 2 Some drift during photography. Poor image quality. Calibration grating used instead of standard grid. few unmylinated 1 or 2 necrotic in series 18-Sep-92 D4 "Box 17, Slot Q-2" 4 "11,073 x" 197 x DR DR and RW "3,540 mm^2" "5,094 mm^2" 41.5 µm^2 1.91% 2198 42 1095 26.1 ±6.18 ±0.96 1.8937E-02 3.3115E-07 59437 59983 100.9% 545 20 23 21 24 28 21 27 21 16 29 26 22 32 33 18 27 16 23 24 35 25 31 22 40 33 21 28 30 23 24 42 25 22 27 30 23 29 25 38 14 26 31 129/J replicate 0.00001 062892C "56,950" "±3,150" 5.5% "97,294" 58.53 Replicate of same section. 1 or 2 19-Apr-95 D4 "Box 17, Slot Q-2" 4 "10,845 x" 192 x RW RW and RW "3,599 mm^2" "5,270 mm^2" 44.8 µm^2 1.01% 2171 22 577 26.2 ±6.65 ±1.45 13 26 30 43 33 30 23 27 16 24 26 34 28 34 20 25 27 28 22 27 24 17 129/J M 1 1 062192B "65,650" "±5,869" "65,650" "±5,869" 8.9% "47,214" 139.05 0.451 "72,031 " JR000690 L 920622 200 23.0 401 17.3 0 2 Denny notes that this mouse looks really beat up. Mouse is a retired breeder. Killed at the Jackson Lab. ">50% unmyelinated. Yes, about half are unmyelinated. Section is presumably close to lamina cribrosa. Excellent fixation. Count is very good" 1 or 2 necrotic in series 18-Sep-92 D3 "Box 17, Slot O-4" 4 "20,611 x" 250 x DR DR and RW "2,949 mm^2" "5,138 mm^2" 12.1 µm^2 1.26% 3904 49 824 16.8 ±10.42 ±1.50 1.9456E-03 2.9636E-08 65046 66454 102.2% " 1,407 " 12 24 33 21 2 3 21 11 15 8 20 2 48 18 33 18 1 13 13 0 0 17 11 19 not available 22 11 19 36 18 13 18 20 22 18 14 11 21 26 21 6 20 16 15 38 15 3 31 1 26 129/J M 1 1 082094E "59,344" "±2,831" "59,344" "±2,831" 4.8% "65,474" 90.64 -1.064 JR000690 ? 940620 940820 61 22.0 394 0 3 none 31-Oct-94 10 "Box 20, Slot D-2" 3 "13,075 x" 191 x DR RW and RW "2,389 mm^2" "5,418 mm^2" 31.7 µm^2 1.06% 2066 22 632 28.7 ±6.28 ±1.37 7.7559E-03 1.3069E-07 55944 60449 108.1% " 4,506 " 22 18 32 38 26 32 24 24 33 30 33 32 26 28 34 26 28 28 35 34 12 37 129/J M 1 1 112194D "62,805" "±3,087" "62,805" "±3,087" 4.9% "56,655" 110.85 -0.232 JR000690 ? 941017 941121 35 17.0 395 0 4 Albino none 2-Feb-95 8 "Box 37, Slot M-2" 4 "13,156 x" 193 x TK RCS and RW "2,120 mm^2" "5,418 mm^2" 31.3 µm^2 1.11% 1810 20 694 34.7 ±7.43 ±1.71 6.9371E-03 1.1046E-07 61004 64252 105.3% " 3,249 " 38 48 25 28 36 28 41 44 48 26 38 42 26 34 37 30 39 28 28 30 129/J M 1 1 112194E "66,697" "±4,710" "66,697" "±4,710" 7.1% "69,246" 96.32 0.703 JR000690 ? 941017 941121 35 16.5 398 0 4 Albino none 2-Feb-95 9 "Box 37, Slot N-2" 4 "13,156 x" 194 x TK RCS and RW "2,609 mm^2" "5,418 mm^2" 31.3 µm^2 0.90% 2212 20 603 30.2 ±9.28 ±2.13 3.1646E-03 4.7447E-08 70862 63087 89.0% " 7,775 " 43 26 27 38 14 29 35 31 28 41 29 17 37 36 45 11 29 35 32 20 129/J original F 1 1 112194F "68,409" "±2,365" "68,154" "±3,329" 4.9% "53,081" 128.39 1.114 JR000690 ? 941017 941121 35 14.8 390 1 4 Albino none 3-Feb-95 12 "Box 37, Slot O-2" 4 "13,156 x" 194 x TK RW and RW "1,995 mm^2" "5,418 mm^2" 31.3 µm^2 1.24% 1696 21 844 40.2 ±8.78 ±1.96 1.2840E-02 1.8769E-07 72678 64218 88.4% " 8,460 " 27 54 41 40 41 43 55 36 37 37 31 44 48 45 41 55 44 21 37 30 37 129/J replicate 0.00001 112194F "68,669" "±3,361" 4.9% "53,678" 127.93 Replicate of a different section none 2-May-95 12 "Box 37, Slot O-4" 4 "12,909 x" 247 x RW RW and RW "3,265 mm^2" "5,270 mm^2" 31.6 µm^2 1.30% 1697 22 890 40.5 ±9.07 ±1.98 50 35 44 38 23 50 60 38 48 36 34 38 53 30 47 40 31 42 52 36 32 33 129/J not countable M 082094D JR000690 940620 940820 61 21.0 422 3 Fixation too poor to count. Good data on brain weight. 129/J not countable M 082094F JR000690 940620 940820 61 20.0 404 3 Fixation too poor to count. Good data on brain weight. 129/J not countable M 082094G JR000690 940620 940820 61 21.0 411 3 Fixation too poor to count. Good data on brain weight. 129/J not countable M 082094H JR000690 940620 940820 61 22.5 415 3 Fixation too poor to count. Good data on brain weight. Brain reweighed by Toya at 0.4147 gm. 129.AKR-bs/bs 5 070993C "43,466" "±2,531" "43,466" "±2,531" 5.8% "71,948" 60.41 930709 not pefused here dup Blind sterile homozygous mutant on a 129.AKR congenic hybrid. 2 necrotic fibers 29-Nov-94 "12,931 x" 190 x DR RW and DR "2,588 mm^2" "5,418 mm^2" 32.4 µm^2 1.17% 2220 26 509 19.6 ±5.70 ±1.14 7.0552E-03 1.6231E-07 39793 47137 118.5% " 7,344 " 19 16 16 15 18 18 21 22 22 18 26 15 31 7 26 19 25 16 18 29 23 21 24 22 7 15 129.AKR-bs/+ F 081094F 940328 940810 135 17.0 428 Normal heterozygote carrier of blind sterile on a 129.AKR congenic hybrid. Injected with HRP. Good perfusion 129.AKR-bs/+ M 081094G 940709 940810 32 14.0 433 1 Normal heterozygote carrier of blind sterile on a 129.AKR congenic hybrid. Injected with HRP. Good perfusion 129.AKR-bs/+ F 081094H 6.7% 940709 940810 32 14.0 428 1 Normal heterozygote carrier of blind sterile on a 129.AKR congenic hybrid. Injected with HRP. Poor perfusion 129BL6F1-dy/dy F 3 071593E "68,757" "±6,392" "68,757" "±6,392" 9.3% "126,587" 54.32 930525 930715 51 1 1 Dystonia muscularis carried on 129/ReJ C57BL/6J F1 A3 "Box 28, Slot L-9" 3 "13,077 x" 193 x TK RW and RW "4,730 mm^2" "5,418 mm^2" 31.7 µm^2 0.60% 3996 24 413 17.2 ±7.67 ±1.60 1.7559E-03 2.5537E-08 81241 56270 69.3% " 24,972 " 17 22 5 17 13 28 29 21 14 16 3 21 10 20 19 37 14 17 12 14 8 13 25 18 129BL6F1-dy/dy M 3 071593F "71,929" "±6,537" "71,929" "±6,537" 9.1% "103,832" 69.27 930525 930715 51 0 1 Dystonia muscularis carried on 129/ReJ C57BL/6J F1 B1 "Box 28, Slot M-2" 2 "13,077 x" 193 x TK RW and RW "3,880 mm^2" "5,418 mm^2" 31.7 µm^2 0.58% 3277 19 417 21.9 ±8.46 ±1.99 1.7768E-03 2.4703E-08 73083 70643 96.7% " 2,440 " 29 21 11 20 18 27 13 26 40 18 20 17 13 35 30 8 31 22 18 A/J score necrotic F 1 1 062892F "51,238" "±1,362" "±3,602" 8 "49,303" "±2,752" "49,303" "±2,752" 5.6% "61,583" 80.06 -0.537 "44,779 " JR000646 JAX307401 R 395 920629 200 27.0 401 15.6 1 3 "This is the re-shoot. A retired breeder from the Research facility at Jackson Lab, room 13, Sect 40. Killed at the Jackson Lab." Few unmyelinated 15-Mar-93 A3 "Box 17, Slot B-2" 3 "12,995 x" 192 x DR DR and RW "2,279 mm^2" "5,313 mm^2" 31.5 µm^2 1.38% 1958 27 680 25.2 ±7.17 ±1.41 6.7597E-03 1.3711E-07 51809 56076 108.2% " 4,266 " 31 27 33 16 25 12 28 19 19 40 39 21 23 36 33 25 12 26 21 27 23 21 31 24 23 20 25 A/J M 1 1 062192D worse "49,533" "±2,599" "49,533" "±2,599" 5.2% "62,036" 79.84 -0.473 JR000646 ? 920621 200 26.0 420 0 3 "Perfusion went badly. Recounted Dec 94 by Rob, but probably still a slight undercount. Keep data. A retired breeder from the Research facility at Jackson Lab, room 13, Sect 40. Killed at the Jackson Lab." Perhaps 1 necrotic 30-Oct-94 A2 "Box 20, Slot A-1" "13,080 x" 193 x DR RW and RW and RW "2,312 mm^2" "5,418 mm^2" 31.7 µm^2 1.07% 1959 21 531 25.3 ±5.93 ±1.33 7.6977E-03 1.5541E-07 50796 48132 94.8% " 2,664 " 21 31 20 23 31 26 30 32 24 32 26 22 22 15 19 31 37 25 14 23 27 A/J score necrotic M 1 1 062192C "49,755" "±3,144" "49,755" "±3,144" 6.3% "56,209" 88.52 -0.411 "61,765 " JR000646 JAX307401 R 920622 200 26.0 NA 14.4 0 3 "A retired breeder. A retired breeder from the Research facility at Jackson Lab, room 13, Sect 40. Killed at the Jackson Lab." "<1% unmyelinated. Brain cut, not weighed" 28-Sep-93 D5 "Box 17, Slot I-1" "14,025 x" 256 x DR DR and RW "3,692 mm^2" "5,103 mm^2" 25.9 µm^2 1.29% 2167 28 643 23.0 ±7.54 ±1.45 5.2202E-03 1.0492E-07 55366 54173 97.8% " 1,192 " 34 30 36 30 24 19 25 24 29 23 26 8 18 30 18 17 33 33 19 12 23 20 15 20 7 25 18 27 A/J replicate F 1 1 021493F "51,298" "±2,335" "49,045" "±2,562" 5.2% "51,227" 95.74 0.017 "57,918 " JR000646 L 930215 35 17.0 357 12.2 1 4 Third sample by Denny on the original thin section also counted the first time. B5 "Box 20A, Slot I-1" 3 "13,011 x" 194 x DR DR and RW "1,922 mm^2" "5,418 mm^2" 32.0 µm^2 1.75% 1601 28 858 30.6 ±8.32 ±1.60 9.7566E-03 1.9019E-07 48064 50830 105.8% " 2,766 " 43 29 39 34 38 18 32 33 29 10 24 30 24 33 25 27 13 30 38 28 31 35 31 46 38 24 36 40 A/J replicate 2 0.00001 021493F "46,566" "±5,671" 12.2% "47,313" 98.42 JR000646 L 930215 dup Second sample by Rob of an adjacent thin section. Low contrast. "A completely independent analysis of an adjacent thin section on the same grid by Rob. Focus on some micrographs is poor, but count is good." 26-Aug-94 B5 "Box 20A, Slot I-1" "12,834 x" 246 x RW DR and RW "2,856 mm^2" "5,418 mm^2" 32.9 µm^2 1.11% 1438 16 518 32.4 ±15.27 ±3.94 52720 51950 98.5% 770 30 7 46 29 46 28 15 55 20 40 15 23 19 44 45 56 A/J original 0.00001 021493F "56,667" "±3,309" 5.8% "51,642" 109.73 L First sample by Denny. Recalibrated nerve cross-sectional area. From the Jackson Lab production colony. B5 "Box 20A, Slot I-1" "13,098 x" 245 x DR DR and RW "3,090 mm^2" "5,324 mm^2" 31.0 µm^2 1.26% 1664 21 715 34.0 ±8.89 ±1.99 26.0 35.0 39.0 27.0 32.0 25.0 46.0 48.0 28.0 25.0 30.0 31.0 25.0 56.0 35.0 32.0 31.0 38.0 44.0 22.0 40.0 A/J original M 1 1 091394G "56,720" "±2,005" "55,542" "±2,770" 5.0% "53,730" 103.37 1.522 JR000646 L 940914 100 24.0 360 0 2 Albino. From The Jackson Laboratory production colony. 26-Aug-94 15 or 16 "Box 30, Slot L-3" 3 "12,968 x" 187 x DR DR and RW "1,878 mm^2" "5,418 mm^2" 32.2 µm^2 1.38% 1668 23 766 33.3 ±7.79 ±1.66 1.4754E-02 2.6012E-07 57951 52910 91.3% " 5,041 " 38 36 48 31 35 18 27 16 26 41 37 39 26 34 46 35 29 30 36 36 41 33 28 A/J replicate 0.00001 091394G "58,018" "±2,906" 5.0% "53,675" 108.09 JR000646 L 940914 dup Redone again by Rob 19-Oct-94 15 or 16 "Box 30, Slot L-3" "16,280 x" 227 x RW RW and RW "2,761 mm^2" "5,418 mm^2" 20.4 µm^2 0.80% 2626 21 464 22.1 ±4.95 ±1.11 56321 59339 105.4% " 3,018 " 24 25 double exposure 26 26 26 32 25 22 15 28 25 23 21 14 24 22 19 21 18 15 13 A/J M 1 1 100494M "50,530" "±2,830" "50,530" "±2,830" 5.6% "58,480" 86.41 -0.196 JR000646 ? 941004 52 21.5 418 0 1 Albino. From The Jackson Laboratory production colony. none 2-Dec-94 6 "Box 34, Slot B-2" 4 "13,037 x" 193 x TK RW and RW "2,179 mm^2" "5,418 mm^2" 31.9 µm^2 1.20% 1834 22 606 27.5 ±7.07 ±1.54 6.6104E-03 1.3082E-07 52196 48861 93.6% " 3,335 " 30 28 34 16 30 34 27 16 23 25 26 21 11 31 29 35 39 22 34 30 30 35 A/J original M 1 1 100494N "56,449" "±4,946" "56,449" "±4,946" 8.8% "57,427" 98.30 1.447 JR000646 ? 941004 52 22.5 431 0 1 Albino. From The Jackson Laboratory production colony. 1 necrotic on 0033 4-Dec-94 8 "Box 34, Slot C-3" 4 "13,055 x" 193 x TK RW and RW "2,139 mm^2" "5,418 mm^2" 31.8 µm^2 0.89% 1806 16 500 31.3 ±10.61 ±2.74 2.4611E-03 4.3599E-08 59383 53513 90.1% " 5,871 " 44 43 39 34 28 27 25 23 35 40 48 26 33 32 11 12 A/J please replicate 1E-08 100494N A/J original F 1 1 100494O "46,312" "±2,059" "46,312" "±2,059" 4.4% "51,333" 90.22 -1.367 JR000646 ? 941004 52 13.8 376 1 1 Albino. From The Jackson Laboratory production colony. All values triple-checked. none 4-Dec-94 9 "Box 34, Slot D-2" 4 "13,055 x" 193 x TK RW and RW "1,912 mm^2" "5,418 mm^2" 31.8 µm^2 1.36% 1615 22 631 28.7 ±5.84 ±1.27 1.1449E-02 2.4722E-07 48146 44476 92.4% " 3,670 " 28 36 27 20 28 29 34 32 24 30 18 22 26 23 25 35 26 32 30 27 37 42 A/J please replicate 1E-08 100494O A/J ordered March 95 for breeding F breeder A/J ordered March 95 for breeding F breeder AKR/J original F 1 1 092092A "62,788" ±890 "±1,990" 6 "63,233" "±2,341" "66,131" "±4,193" 6.3% "68,295" 96.83 0.224 JR000648 R GET WEIGHTS 920806 920920 45 NA 428 1 1 Counted using two separate counting frames. Data are taken from the second count using the standard frame. <15% unmyelinated none 4-Nov-92 A3 "Box 14, Slot L-1" 3 "13,250 x" 255 x DR RW and RW "4,450 mm^2" "5,270 mm^2" 30.0 µm^2 1.32% 2275 30 872 29.1 ±9.92 ±1.84 1.1935E-02 1.8875E-07 64025 80538 125.8% " 16,513 " 31 28 30 edge blank 27 25 34 28 38 27 21 40 28 39 18 5 21 25 41 46 43 41 19 37 19 28 15 23 18 45 32 AKR/J replicate 0.00001 092092A "63,588" "±2,822" 4.4% "72,165" 88.11 This is the second interleave of the high resolution count by Richelle. The lower density is not due to any difficulty in counting or resolving axons. none 15-Mar-95 A3 "Box 14, Slot L-3" "10,913 x" 193 x RCS RCS and RCS "2,697 mm^2" "5,418 mm^2" 45.5 µm^2 3.66% 1586 58 2325 40.1 ±13.43 ±1.78 41 22 30 24 6 33 28 7 45 43 50 45 44 48 45 45 54 27 72 60 52 47 42 30 45 25 31 20 cal cal 46 19 50 27 40 43 62 56 58 57 37 35 36 24 45 57 48 46 57 49 40 22 25 30 47 43 42 42 49 32 AKR/J replicate 2 0.00001 092092A "62,091" "±2,334" 3.8% "74,419" 83.43 This is the first interleave of the high resolution count with about 60 sample photographs by Rob none 15-Mar-95 A3 "Box 14, Slot L-3" "10,842 x" 191 x RW RW and RCS "2,711 mm^2" "5,418 mm^2" 46.1 µm^2 3.65% 1615 59 2269 38.5 ±11.01 ±1.45 38 36 36 41 38 31 37 43 34 39 52 49 41 42 59 34 40 21 60 46 47 36 33 34 27 32 36 33 23 44 10 59 46 57 26 46 61 44 35 48 34 39 48 46 48 55 34 29 27 23 37 15 38 23 41 31 50 33 24 AKR/J original F 1 1 092092B "60,063" "±1,913" "62,660" "±4,625" 7.4% "80,965" 77.39 -1.370 "53,457 " JR000648 R 920806 920920 45 NA 427 14.7 1 1 Check birth date "<5% unmyelinated. Calibrations redo by Rob, March 1995." none 4-Nov-92 A6 "Box 14, Slot O-2" 3 "13,312 x" 255 x DR DR and RW "5,256 mm^2" "5,012 mm^2" 28.3 µm^2 1.26% 2863 36 788 21.9 ±9.56 ±1.62 1.6887E-02 2.8115E-07 62402 68743 110.2% " 6,341 " 21 24 24 7 13 0 22 34 21 26 28 27 26 25 31 28 edge 29 28 25 28 0 30 23 31 27 26 15 18 4 4 22 24 8 edge 33 36 20 AKR/J replicate 0.00001 092092B "60,024" "±2,101" 3.5% "84,177" 71.31 "This is the first interleaved set of about 60 micrographs (0.5 rotations right to left, 1 rotation, between rows). Same section as the second interleaved set below. Different thin section from the original estimate." none 17-Mar-95 A6 "Box 14, M-4" "10,996 x" 248 x RW RW and RW "5,193 mm^2" "5,418 mm^2" 44.8 µm^2 3.46% 1878 65 2077 32.0 ±8.95 ±1.12 cal 21 19 33 29 30 22 29 52 37 47 31 41 25 36 32 38 39 23 39 31 42 35 42 37 33 47 40 27 21 21 38 42 47 35 24 40 12 40 35 35 18 33 14 24 30 30 27 31 36 43 31 29 37 26 12 23 40 34 34 10 36 33 32 38 29 AKR/J replicate 2 0.00001 092092B "58,384" "±3,458" 5.9% "82,661" 70.63 "This is the second interleaved set of about 30 micrographs (0.5 rotations right to left, 1 rotation, between rows). Series ends at about micrograph #103" none 17-Mar-95 A6 "Box 14, M-4" "10,991 x" 248 x RW RW and RW "5,083 mm^2" "5,418 mm^2" 44.8 µm^2 1.68% 1843 31 982 31.7 ±10.28 ±1.88 39 25 10 20 33 35 37 25 31 28 32 44 44 36 43 37 27 45 36 11 23 34 46 33 29 22 40 30 36 44 7 AKR/J replicate 3 0.00001 092092B "60,325" "±3,079" 5.1% "83,650" 72.12 "This is the third interleaved set of 29 micrographs (0.5 rotations right to left, 1 rotation, between rows). Series ends at about micrograph #132." none 17-Mar-95 A6 "Box 14, M-4" "10,968 x" 247 x RW RW and RW "5,099 mm^2" "5,418 mm^2" 45.0 µm^2 1.56% 1857 29 942 32.5 ±8.77 ±1.66 14 28 25 34 33 39 33 23 32 26 39 33 27 35 31 29 32 49 34 47 42 21 39 28 27 40 30 53 19 AKR/J get body weight F 1 1 021493G "65,143" "±2,754" "69,029" "±4,536" 6.6% "69,410" "15,277" "1,971" 99.45 1.184 "61,755 " JR000648 R 930215 35 NA 465 14.7 1 2 Low mag cal and photomontage redone by Rob. 4-6 weeks 9-Mar-93 C2 "Box 20A, Slot J-2" 4 "13,123 x" 245 x DR and RW DR and RW "4,176 mm^2" "5,330 mm^2" 30.9 µm^2 1.20% 2243 27 831 30.8 ±10.31 ±2.02 8.9206E-03 1.3694E-07 63758 74701 117.2% " 10,943 " 32 24 30 9 25 34 34 4 19 26 24 44 21 31 47 34 41 49 31 33 34 43 35 38 28 35 26 AKR/J 0.00001 021493G "65,893" "±3,466" 5.3% "63,309" 104.08 JR000648 R 930215 dup dup "Again, a complete new set of data from same slot but a third thin section. Restained to improve contrast. " none 7-Mar-95 C2 "Box 20A, Slot J-2" "13,054 x" 194 x RW RW and RW "2,372 mm^2" "5,418 mm^2" 31.8 µm^2 1.10% 1991 22 728 33.1 ±7.98 ±1.74 62539 68666 109.8% " 6,128 " 29 42 52 26 33 40 36 34 28 28 31 33 35 40 17 39 41 26 22 39 23 34 AKR/J 0.00001 021493G "61,273" "±3,924" 6.4% "67,178" 91.21 JR000648 R 930215 dup dup "A second set of data from same slot but a second thin section. Low contrast, but good tissue. High mag calibration is asymmetric. This probably account for some of the variation." none 15-Aug-94 C2 "Box 20A, Slot J-2" "12,897 x" 246 x RW RW and RW "4,067 mm^2" "5,418 mm^2" 32.6 µm^2 1.16% 2062 24 713 29.7 ±9.12 ±1.90 56029 66513 118.7% " 10,484 " 33 40 38 39 29 36 32 39 24 24 29 26 28 44 26 14 34 28 35 29 8 31 37 10 AKR/J M 1 1 040494I "64,683" "±4,484" "64,683" "±4,484" 6.9% "119,298" 54.22 0.952 940904 100 NA 479 0 3 15-Sep-94 "Box 28, Slot N-1" "13,007 x" 247 x RW RW and RW "7,256 mm^2" "5,418 mm^2" 32.0 µm^2 0.59% 3725 22 382 17.4 ±5.52 ±1.20 3.3695E-03 5.2093E-08 70733 63030 89.1% " 7,704 " 9 17 15 9 15 15 24 23 18 16 14 12 28 22 13 20 13 27 12 16 19 25 AKR/J original F 1 1 040494J "61,064" "±2,334" "65,142" "±4,411" 6.8% "99,768" 65.29 -0.867 940904 100 NA 496 1 3 15-Sep-94 15/16 "Box 28, Slot O-2" 4 "13,107 x" 247 x RW RW and RW "6,102 mm^2" "5,418 mm^2" 31.5 µm^2 0.70% 3164 22 453 20.6 ±6.39 ±1.39 1.1742E-02 1.9229E-07 68715 67215 97.8% " 1,500 " 19 19 21 20 12 38 25 19 17 5 16 24 21 14 26 19 18 27 23 23 26 21 AKR/J replicate 0.00001 040494J "59,325" "±2,751" 4.6% "100,830" 58.84 Replicate of different section started by RCS and finished next day by Rob and Toya. This is the first interleaved set of about 80 micrographs none 18-Mar-95 15/16 "Box 28, Slot O-4" "10,962 x" 193 x RCS and RW RW and RW "3,757 mm^2" "5,418 mm^2" 45.1 µm^2 3.49% 2236 78 2069 26.5 ±10.79 ±1.23 12 17 13 11 22 13 7 17 16 19 19 19 20 22 9 13 27 28 39 38 26 29 10 9 10 18 26 45 13 24 49 35 29 27 19 21 24 13 43 30 32 49 53 28 29 28 34 24 30 39 22 14 35 43 30 38 28 29 17 27 16 29 47 24 28 40 25 38 19 36 29 23 32 37 46 30 33 27 AKR/J replicate 2 0.00001 040494J "61,430" "±4,259" 6.9% "100,741" 60.98 Replicate of same section (second interleaved set) of about 40 micrographs by Toya. none 18-Mar-95 15/16 "Box 28, Slot O-4" "10,966 x" 193 x TK RW and RW "3,764 mm^2" "5,418 mm^2" 45.1 µm^2 1.79% 2236 40 1099 27.5 ±11.90 ±1.91 10 8 13 14 29 30 32 16 14 50 45 35 17 23 18 20 24 54 44 33 29 4 39 42 50 26 28 31 32 31 22 37 31 22 29 30 32 23 14 18 AKR/J F 1 1 040494K "62,543" "±4,668" "62,543" "±4,668" 7.5% "96,249" 64.98 -0.123 940904 100 NA 471 1 3 15-Sep-94 "Box 28, Slot P-1" 4 "13,046 x" 247 x RW RW and RW "5,886 mm^2" "5,418 mm^2" 31.8 µm^2 0.63% 3024 19 393 20.7 ±6.55 ±1.54 3.0300E-03 4.8447E-08 71121 59910 84.2% " 11,211 " 11 20 23 25 20 26 30 16 9 26 9 16 21 19 24 22 25 33 18 B6C3Fe-+/rl F 1 5 910311H "63,357" "±4,148" "±9,276" 6 "69,879" "±2,440" "69,879" "±2,440" 3.5% "77,914" 89.69 0.703 JR0235 L 901024 910311 138 No data 1 1 "A reeler heterozygote. All calibrations etc redone by Rob, Dec 1994." 1-May-91 B2 "Box 11, Slot O-2" 3 "11,175 x" 391 x DT 4-30-91 DT and DT and RW "11,932 mm^2" "5,382 mm^2" 43.1 µm^2 1.88% 1808 34 1314 38.6 ±7.75 ±1.35 1.2089E-02 1.7300E-07 69664 70089 100.6% 425 42 42 25 48 33 31 30 40 38 44 46 42 47 29 33 43 29 41 38 39 36 39 56 34 46 39 44 39 42 46 52 32 22 27 B6C3Fe-rl/rl F 1 5 900607.1 "55,038" "±2,184" "55,038" "±2,184" 4.0% "79,505" 69.23 -0.897 JR0235 Reeler 1 "L, near chiasm" 900607 100 No data 1 2 "Case 2503. Reeler homozygote. All calibrations and areas redone by Rob, Dec 94." Reeler homozygote. 6-Mar-91 B4 "Box 11, Slot C-1" 3 "11,037 x" 385 x DT 2-14-91 DT and RW "11,767 mm^2" "5,250 mm^2" 43.1 µm^2 1.95% 1845 36 1074 29.8 ±7.00 ±1.18 1.1867E-02 2.1562E-07 56676 53396 94.2% " 3,280 " 28 20 32 29 21 23 26 22 31 21 30 34 25 27 26 32 20 28 33 26 29 49 35 35 19 32 36 38 25 36 25 29 46 41 34 31 B6C3Fe-rl/rl original F 1 5 900607.2 "58,139" "±1,775" "53,447" "±5,089" 9.5% "79,044" 67.62 -0.563 JR0235 Reeler #2 "L, near lamina cribrosa" 900607 100 No data 1 2 "Done by Denny at 12,000 on section adjacent to that used by Daphne. Good data. All calibrations and areas by Rob. Daphne's original count was about 68,000, but this high value due to miscalibration. " All of these reeler animals from Dan's colony at UT Memphis. 26-Sep-94 C2 "Box 12, Slot Q-2" 2 "13,003 x" 194 x "DR, Sept 25, 1994" DR and RW "2,962 mm^2" "5,418 mm^2" 32.0 µm^2 0.85% 2467 21 455 21.7 ±9.23 ±2.06 1.9377E-02 3.3329E-07 59202 48213 81.4% " 10,988 " 37 22 18 3 8 29 22 20 18 33 23 19 12 21 5 27 23 29 26 37 23 B6C3Fe-rl/rl replicate 0.00001 900607.2 "54,297" "±1,894" 3.5% "76,202" 71.25 JR0235 Reeler #2 "L, near lamina cribrosa" 900607 No data dup "Done again by Denny at 12,000 on same thin section used by Daphne, but contrast on Denny's negatives is quite low. Count seems fine to Rob. All calibrations and areas by Rob." Reeler homozygote. Very low contrast negatives. None 31-Dec-94 C2 "Box 12, Slot Q-2" "13,219 x" 194 x "DR on Dec 29, 1994" RW and RW "2,879 mm^2" "5,418 mm^2" 31.0 µm^2 0.90% 2458 22 486 22.1 ±3.53 ±0.77 55412 53178 96.0% " 2,234 " 26 30 18 19 23 22 19 18 25 21 22 26 24 24 20 20 17 20 21 29 23 19 B6C3Fe-rl/rl relicate 2 0.00001 900607.2 "53,658" "±3,052" 5.7% "73,911" 72.60 JR0235 Reeler #2 "L, near lamina cribrosa" 900607 No data dup Done yet again by Rob on middle thin section. Grid was restained first to improve contrast. Good count despite marginal fixation. 9-Mar-95 C2 "Box 12, Slot Q-2" "13,029 x" 194 x RW RW and RW "2,770 mm^2" "5,418 mm^2" 31.9 µm^2 1.25% 2316 29 672 23.2 ±6.97 ±1.32 19 26 26 25 25 20 19 19 11 25 24 29 18 22 22 29 3 19 24 34 25 24 27 29 31 11 25 23 38 B6C3Fe-rl/rl Right 0.00001 900607.2 "62,219" "±3,032" 4.9% "83,376" 74.63 JR0235 Reeler #2 "R, near chiasm" 900607 No data dup This is the right optic nerve near the optic chiasm None 31-Dec-94 D2 "Box 2, Slot I-1" "13,219 x" 194 x "DR on Dec 29, 1994" RW and RW "3,150 mm^2" "5,418 mm^2" 31.0 µm^2 0.82% 2689 22 509 23.1 ±5.17 ±1.13 65273 59161 90.6% " 6,112 " 19 16 22 23 23 23 29 19 19 19 16 27 33 19 17 27 27 23 35 22 27 24 B6C3Fe-rl/rl needs to be replicated F 1 5 900607.4 "78,574" "±4,331" "78,574" "±4,331" 5.5% "79,974" 98.25 1.641 JR0235 Reeler #4 R 900607 100 No data 1 2 "Redone Oct 94 by Rob at higher magnification to improve count. Initial count was 62,729. Poor images. Area of nerve in 1st set was 69385 mu2. Too low." Reeler homozygote. Section mid-orbital. Tiny mama Reeler #4 24-Oct-94 A2 "Box 3, Slot F-10" 2 "16,322 x" 222 x RW RW and RW "3,943 mm^2" "5,355 mm^2" 20.1 µm^2 0.50% 3978 20 395 19.8 ±4.74 ±1.09 4.4101E-03 5.6127E-08 81158 75986 93.6% " 5,172 " 26 20 17 21 20 19 27 15 15 19 13 31 20 21 23 20 12 15 18 23 B6C3Fe-rl/rl F 1 5 910311F "62,863" "±1,964" "62,863" "±1,964" 3.1% "68,868" 91.28 -0.053 JR0235 R 910311 100 No data 1 3 Case 2562. Area of nerve and photo double checked. All calibrations redone by Rob Dec 94. Reeler homozygote. 3-Apr-91 D4 "Box 11, Slot G-1" 2 "10,002 x" 360 x DT 4-1-91 DT and DT "8,940 mm^2" "5,250 mm^2" 52.5 µm^2 2.44% 1312 32 1533 47.9 ±8.33 ±1.50 1.6823E-02 2.6762E-07 61209 65362 106.8% " 4,154 " 57 51 56 63 48 42 41 edge 41 53 42 40 40 51 47 43 39 51 30 56 47 47 61 59 40 40 43 51 57 60 51 33 53 B6C3Fe-rl/rl F 1 5 910311G "55,648" "±3,058" "55,648" "±3,058" 5.5% "67,182" 82.83 -0.831 JR0235 L 910311 100 No data 1 3 "Older than 100 days. May be a slight undercount due to difficulty counting unmyelinated fibers. All calibrations redone by Rob, Dec 94." Reeler homozygote. 29-Dec-94 A4 "Box 12, Slot S-2" 2 "11,150 x" 376 x DT 5-14-91 DT and DT and RW "9,489 mm^2" "5,408 mm^2" 43.5 µm^2 1.88% 1544 29 1045 36.0 ±10.48 ±1.98 6.1617E-03 1.1073E-07 52601 59395 112.9% " 6,793 " 43 38 45 44 37 42 edge 34 55 45 39 20 43 16 26 35 52 37 36 19 31 20 25 28 43 57 38 37 30 30 B6AxCF1/JGo original F 1 3 120594I "64,854" "±2,408" "±5,898" 7 "59,256" "±2,721" "58,994" "±3,043" 5.2% "78,130" 75.51 -0.949 ? 468 941205 35 15.8 440 1 1 "Case 19. C57BL/6J female # 1 (64,245) or 2 (59,267) by BALB/c male #7" Hybrids generated at UT Memphis using Jackson Lab production stock 25-Jan-95 20 "Box 36, Slot O-2" 4 "13,189 x" 193 x RCS RW and RW "2,919 mm^2" "5,418 mm^2" 31.1 µm^2 1.08% 2508 27 635 23.5 ±6.19 ±1.21 8.3141E-03 1.4031E-07 56975 61165 107.4% " 4,190 " 26 30 28 11 22 28 20 30 16 23 13 20 17 20 32 29 17 16 24 25 25 34 22 19 26 32 30 B6AxCF1/JGo replicate 0.00001 120594I better "60,299" "±6,074" 10.1% "76,029" 79.31 Replicate on different section by Rob counted by Evan Williams 19-Apr-95 20 "Box 36, Slot O-2" "16,032 x" 192 x RW EGW and RW "2,813 mm^2" "5,270 mm^2" 20.5 µm^2 0.62% 3708 23 374 16.3 ±7.68 ±1.64 19 12 29 21 11 8 11 22 18 9 18 28 26 21 0 3 17 11 15 14 28 18 15 B6AxCF1/JGo F 1 3 120594J "63,580" "±4,408" "63,580" "±4,408" 6.9% "59,250" 107.31 -0.216 ? 941205 35 15.3 430 1 1 Case 20. C57BL/6J female # 1 or 2 by BALB/c male #7 Hybrids generated at UT Memphis using Jackson Lab stock 17-Jan-95 2 "Box 35, Slot N-1" 3 "18,024 x" 196 x TK RW and RW "2,267 mm^2" "5,418 mm^2" 16.7 µm^2 1.35% 3553 48 859 17.9 ±8.51 ±1.24 3.3418E-03 5.2560E-08 65725 61432 93.5% " 4,293 " 14 16 3 21 5 12 0 17 13 27 12 4 25 28 25 14 30 28 22 16 13 14 22 18 24 23 28 40 26 15 25 11 10 16 11 12 18 19 17 5 21 18 14 39 21 23 16 8 B6AxCF1/JGo original M 1 3 120594K "56,718" "±2,436" "57,399" "±4,761" 8.3% "47,271" 121.43 -1.379 ? 941205 35 17.5 445 0 1 Case 16. C57BL/6J female # 1 or 2 by BALB/c male #7 Hybrids generated at UT Memphis using Jackson Lab stock 11-Jan-95 3 "Box 35, Slot O-4" 2 "18,072 x" 146 x TK TK and RW "1,002 mm^2" "5,418 mm^2" 16.6 µm^2 0.49% 2850 14 282 20.1 ±6.02 ±1.67 1.0297E-02 1.8155E-07 48800 64000 131.1% " 15,200 " 26 16 15 21 22 15 17 18 22 23 33 8 25 21 replicate 0.00001 120594K "56,477" "±2,835" 5.0% "51,577" 109.50 Different thin section on same slot. 21-Apr-95 3 "Box 35, Slot O-4" "16,038 x" 194 x RW RW and RW "1,938 mm^2" "5,270 mm^2" 20.5 µm^2 0.91% 2517 23 516 22.4 ±5.28 ±1.13 32 17 15 26 25 21 15 24 26 25 19 25 20 29 15 15 24 29 19 28 16 28 23 B6AxCF1/JGo original M 1 3 120594L "69,454" "±5,199" "69,559" "±7,026" 10.1% "70,018" 99.34 0.780 ? 941205 35 19.3 454 0 1 Case 17. C57BL/6J female # 1 or 2 by BALB/c male #7 11-Jan-95 5 "Box 35, Slot P-1" "18,180 x" 146 x TK TK and RW "1,484 mm^2" "5,418 mm^2" 16.4 µm^2 0.49% 4271 21 342 16.3 ±7.36 ±1.64 2.6978E-03 3.8843E-08 71396 69858 97.8% " 1,538 " 32 20 29 19 20 15 21 14 10 14 15 24 5 13 7 12 2 18 22 12 18 replicate 0.00001 120594L "69,328" "±7,730" 11.2% "76,420" 90.72 Replicate by Rob of different thin section on same grid. Beautiful tissue. 13-Apr-95 5 "Box 35, Slot P-1" 4 "16,032 x" 191 x RW RW and RW "2,796 mm^2" "5,270 mm^2" 20.5 µm^2 0.54% 3727 20 372 18.6 ±9.04 ±2.07 21 15 27 9 12 11 10 18 20 17 31 24 20 33 8 7 27 3 30 29 B6AxCF1/JGo original M 1 3 120594M "65,825" "±4,151" "60,950" "±8,711" 14.3% "71,698" 85.01 0.165 ? 941205 35 19.3 458 0 1 Case 18. C57BL/6J female # 1 or 2 by BALB/c male #7 11-Jan-95 7 "Box 35, Slot Q-4" 3 "18,321 x" 197 x TK TK and RW "2,785 mm^2" "5,418 mm^2" 16.1 µm^2 0.41% 4442 18 247 13.7 ±8.09 ±1.96 4.0453E-03 6.1455E-08 68980 51857 75.2% " 17,123 " 2 12 21 20 20 17 20 14 2 28 20 3 5 9 9 13 7 25 replicate 0.00001 120594M "67,257" "±4,721" 7.0% "67,528" 99.60 Replicate by Rob of different thin section on same grid 13-Apr-95 7 "Box 35, Slot Q-4" "16,200 x" 248 x RW RW and RW "4,153 mm^2" "5,270 mm^2" 20.1 µm^2 0.68% 3363 23 460 20.0 ±6.59 ±1.40 18 16 28 8 25 17 8 16 19 17 23 37 16 23 20 24 21 24 15 14 22 30 19 B6AxCF1/JGo M 1 3 010695L "64,938" "±3,005" "64,938" "±3,005" 4.6% "80,594" 80.57 0.014 ? 941201 950106 36 20.5 512 0 2 Case 56. C57BL/6J females #1 or #2 by BALB/c male #7 21-Apr-95 3 "Box 42, Slot O-2" 4 "10,759 x" 194 x RW RW and RW "3,028 mm^2" "5,270 mm^2" 45.5 µm^2 1.24% 1770 22 807 36.7 ±7.78 ±1.70 7.5331E-03 1.1600E-07 31 42 56 44 36 30 31 30 30 29 35 39 52 39 44 33 30 33 37 46 32 28 B6AxCF1/JGo original M 1 3 010695M "74,205" "±5,236" "74,205" "±5,236" 7.1% "76,802" 96.62 1.586 ? 941201 950106 36 20.3 504 0 2 Case 57. C57BL/6J females #1 or #2 by BALB/c male #7 1 necrotic 5-May-95 5 "Box 42, Slot P-1" 3 "13,080 x" 206 x RW RW and RW "3,269 mm^2" "5,270 mm^2" 30.8 µm^2 0.84% 2493 21 625 29.8 ±9.39 ±2.10 2.8424E-03 3.8305E-08 40 43 33 36 40 18 22 28 31 39 11 28 22 32 43 30 10 27 27 36 29 B6AxCF1/JGo please reshoot 1E-08 010695M B6AxCF1/JGo Please photo M 010695N 941201 950106 36 17.0 498 0 2 Case 58. C57BL/6J females #1 or #2 by BALB/c male #7 6 "Box 42, Slot Q" B6AxCF1/JGo F 021395I 950107 950213 37 20.3 477 4 Case 126. C57BL/6J female # 1 or 2 by BALB/c male #7 B6AxCF1/JGo M 021395J 950107 950213 37 18.3 478 4 Case 127. C57BL/6J female # 1 or 2 by BALB/c male #7 B6AxCF1/JGo F 021395K 941101 950213 104 29.5 484 3 Case 28: Parents were B6 female 1 or 2 and BALB/c male #7 B6AxCF1/JGo F 021395L 941101 950213 104 27.0 509 3 Case 29: Parents were B6 female 1 or 2 and BALB/c male #7 B6AxCF1/JGo F 021395M 941101 950213 104 26.5 486 3 Case 30: Parents were B6 female 1 or 2 and BALB/c male #7 B6AxCF1/JGo F 030695N 941101 950306 125 26.5 505 5 Case 25 Agouti coat B6AxCF1/JGo F 030695O 941101 950306 125 28.0 484 5 Case 26 Agouti coat B6AxCF1/JGo F 030695P 941101 950306 125 25.0 480 5 Case 27 Agouti coat B6CF2/JGo F 020795J 453 950101 950207 37 19.8 480 1 or 2 Case 97. albino "Parents were B6CF1 males 21 and 22 and B6CF1 females 25, 26, and 27" "Tissue, not extracted" B6CF2/JGo F 020795K 950101 950207 37 17.3 439 1 or 2 Case 98. albino "Parents were B6CF1 males 21 and 22 and B6CF1 females 25, 26, and 27" "Tissue, not extracted" B6CF2/JGo F 020795L 950101 950207 37 17.0 421 1 or 2 Case 99. albino "Parents were B6CF1 males 21 and 22 and B6CF1 females 25, 26, and 27" "Tissue, not extracted" B6CF2/JGo F 020795M 950101 950207 37 16.0 461 1 or 2 Case 100. albino "Parents were B6CF1 males 21 and 22 and B6CF1 females 25, 26, and 27" "Tissue, not extracted" B6CF2/JGo F 020795N 950101 950207 37 17.5 451 1 or 2 Case 101. albino "Parents were B6CF1 males 21 and 22 and B6CF1 females 25, 26, and 27" "Tissue, not extracted" B6CF2/JGo F 020795O 950101 950207 37 14.5 402 1 or 2 Case 102. albino "Parents were B6CF1 males 21 and 22 and B6CF1 females 25, 26, and 27" "Tissue, not extracted" B6CF2/JGo F 020795P 950101 950207 37 18.5 455 1 or 2 Case 103. albino "Parents were B6CF1 males 21 and 22 and B6CF1 females 25, 26, and 27" "Tissue, not extracted" B6CF2/JGo F 020795Q 950101 950207 37 18.8 452 1 or 2 Case 104. agouti "Parents were B6CF1 males 21 and 22 and B6CF1 females 25, 26, and 27" "Tissue, not extracted" B6CF2/JGo F 020795R 950101 950207 37 17.0 477 1 or 2 Case 105. agouti "Parents were B6CF1 males 21 and 22 and B6CF1 females 25, 26, and 27" "Tissue, not extracted" B6CF2/JGo F 020795S 950101 950207 37 15.3 483 1 or 2 Case 106. agouti "Parents were B6CF1 males 21 and 22 and B6CF1 females 25, 26, and 27" "Tissue, not extracted" B6CF2/JGo F 020795T 950101 950207 37 20.0 486 1 or 2 Case 107. agouti "Parents were B6CF1 males 21 and 22 and B6CF1 females 25, 26, and 27" "Tissue, not extracted" B6CF2/JGo F 020795U 950101 950207 37 16.5 431 1 or 2 Case 108. agouti "Parents were B6CF1 males 21 and 22 and B6CF1 females 25, 26, and 27" "Tissue, not extracted" B6CF2/JGo M 020995A 950101 950209 39 21.5 480 1 or 2 Case 109. Albino "Parents were B6CF1 males 21 and 22 and B6CF1 females 25, 26, and 27" "Tissue, not extracted" B6CF2/JGo M 020995B 950101 950209 39 21.0 459 1 or 2 Case 110. Albino "Parents were B6CF1 males 21 and 22 and B6CF1 females 25, 26, and 27" "Tissue, not extracted" B6CF2/JGo M 020995C 950101 950209 39 22.5 483 1 or 2 Case 111. Albino "Parents were B6CF1 males 21 and 22 and B6CF1 females 25, 26, and 27" "Tissue, not extracted" B6CF2/JGo M 020995D 950101 950209 39 22.0 495 1 or 2 Case 112. Agouti "Parents were B6CF1 males 21 and 22 and B6CF1 females 25, 26, and 27" "Tissue, not extracted" B6CF2/JGo M 020995E 950101 950209 39 20.5 494 1 or 2 Case 113. Agouti "Parents were B6CF1 males 21 and 22 and B6CF1 females 25, 26, and 27" "Tissue, not extracted" B6CF2/JGo M 020995F 950101 950209 39 21.5 506 1 or 2 Case 114. Agouti "Parents were B6CF1 males 21 and 22 and B6CF1 females 25, 26, and 27" "Tissue, not extracted" B6CF2/JGo M 020995G 950101 950209 39 18.5 missing hindbrain 1 or 2 Case 115. Agouti. Part of brain missing. "Parents were B6CF1 males 21 and 22 and B6CF1 females 25, 26, and 27" "Tissue, not extracted" B6CF2/JGo M 020995H 950101 950209 39 21.8 478 1 or 2 Case 116. Agouti "Parents were B6CF1 males 21 and 22 and B6CF1 females 25, 26, and 27" "Tissue, not extracted" B6CF2/JGo M 020995I 950101 950209 39 18.5 462 1 or 2 Case 117. Black "Parents were B6CF1 males 21 and 22 and B6CF1 females 25, 26, and 27" "Tissue, not extracted" B6CF2/JGo F 021395A 950105 950213 39 19.0 472 3 Case 118 "Parents were B6CF1 males 21 and 22 and B6CF1 females 28, 29, and 30" "Tissue, not extracted" B6CF2/JGo M 021395B 950105 950213 39 24.8 486 3 Case 119 "Parents were B6CF1 males 21 and 22 and B6CF1 females 28, 29, and 30" "Tissue, not extracted" B6CF2/JGo F 021395C 950105 950213 39 18.0 472 3 Case 120 "Parents were B6CF1 males 21 and 22 and B6CF1 females 28, 29, and 30" "Tissue, not extracted" B6CF2/JGo F 021395D 950105 950213 39 18.3 450 3 Case 121 "Parents were B6CF1 males 21 and 22 and B6CF1 females 28, 29, and 30" "Tissue, not extracted" B6CF2/JGo M 021395E 950105 950213 39 22.3 474 3 Case 122 "Parents were B6CF1 males 21 and 22 and B6CF1 females 28, 29, and 30" "Tissue, not extracted" B6CF2/JGo F 021395F 950105 950213 39 17.8 455 3 Case 123 "Parents were B6CF1 males 21 and 22 and B6CF1 females 28, 29, and 30" "Tissue, not extracted" B6CF2/JGo M 021395G 950105 950213 39 23.3 506 3 Case 124 "Parents were B6CF1 males 21 and 22 and B6CF1 females 28, 29, and 30" "Tissue, not extracted" B6CF2/JGo M 021395H 950105 950213 39 20.0 467 3 Case 125 "Parents were B6CF1 males 21 and 22 and B6CF1 females 28, 29, and 30" "Tissue, not extracted" B6CF2/JGo M 030695D 950126 950306 39 20.0 439 4 Case 171. Albino "Parents were B6CF1 one female (25, 26, or 27) and male B6CF1 #21 or #22." "Tissue, not extracted" B6CF2/JGo M 030695E 950126 950306 39 19.0 445 4 Case 172. Albino "Parents were B6CF1 one female (25, 26, or 27) and male B6CF1 #21 or #22." "Tissue, not extracted" B6CF2/JGo F 030695F 950126 950306 39 17.0 436 4 Case 173. non-agouti "Parents were B6CF1 one female (25, 26, or 27) and male B6CF1 #21 or #22." "Tissue, not extracted" B6CF2/JGo M 030695G 950126 950306 39 17.0 440 4 Case 174 "Parents were B6CF1 one female (25, 26, or 27) and male B6CF1 #21 or #22." "Tissue, not extracted" B6CF2/JGo M 030695H 950126 950306 39 21.0 443 4 Case 175 "Parents were B6CF1 one female (25, 26, or 27) and male B6CF1 #21 or #22." "Tissue, not extracted" B6CF2/JGo M 030695I 950126 950306 39 21.0 456 4 Case 176 "Parents were B6CF1 one female (25, 26, or 27) and male B6CF1 #21 or #22." "Tissue, not extracted" B6CF2/JGo M 030695J 950126 950306 39 19.8 441 4 Case 177 "Parents were B6CF1 one female (25, 26, or 27) and male B6CF1 #21 or #22." "Tissue, not extracted" B6CF2/JGo F 030695K 950126 950306 39 16.0 399 4 Case 178 "Parents were B6CF1 one female (25, 26, or 27) and male B6CF1 #21 or #22." "Tissue, not extracted" B6CF2/JGo F 030695L 950126 950306 39 19.5 452 4 Case 179 "Parents were B6CF1 one female (25, 26, or 27) and male B6CF1 #21 or #22." "Tissue, not extracted" B6CF2/JGo M 030695M 950126 950306 39 19.5 431 4 Case 180 "Parents were B6CF1 one female (25, 26, or 27) and male B6CF1 #21 or #22." "Tissue, not extracted" BALB/cBy small sample at high mag F 1 1 120594N "57,558" "±1,768" "±4,330" 7 "57,402" "±6,978" "57,402" "±6,978" 12.2% "39,009" 147.15 -0.036 ? 421 941205 30 13.0 380 1 1 Photographed on JEOL 1000 All BALB/cBy from the Jackson Lab production colony. 1 necrotic on 0019 Jan-13-95 9 "Box 35, Slot R-1" 3 "18,151 x" 198 x TK RW and RW "1,535 mm^2" "5,418 mm^2" 16.4 µm^2 0.63% 2372 15 363 24.2 ±11.01 ±2.94 1.2630E-03 2.2002E-08 61589 62551 101.6% 962 44 28 12 18 40 25 17 14 15 39 20 22 10 22 37 BALB/cBy original M 1 1 120594O "59,816" "±3,978" "64,703" "±6,836" 10.6% "59,615" 108.54 0.522 ? 941205 30 15.5 408 0 1 Photographed on JEOL 1000 none Jan-13-95 12 "Box 35, Slot S-2" 4 "17,275 x" 198 x TK RW and RW "2,346 mm^2" "5,418 mm^2" 18.2 µm^2 0.52% 3283 17 335 19.7 ±8.33 ±2.08 4.0154E-03 6.7129E-08 65627 73707 112.3% " 8,080 " 17 15 9 26 18 9 22 21 15 31 32 29 18 10 25 7 31 BALB/cBy replicate 0.00001 120594O "57,313" "±4,892" 8.5% "62,918" 91.09 Replicate of same section none May-2-95 12 "Box 35, Slot S-2" "15,976 x" 247 x RW RW and RW "3,835 mm^2" "5,270 mm^2" 20.6 µm^2 0.69% 3047 21 395 18.8 ±7.18 ±1.61 20 23 13 30 22 16 16 22 31 19 19 7 21 23 17 18 28 6 24 4 16 BALB/cBy original F 1 1 120594P "63,924" "±1,894" "63,924" "±3,788" 5.9% "51,269" 124.68 1.470 ? 941205 30 14.0 413 1 1 Lots of unmyeliated fibers (20%) none Feb-28-95 13 "Box 38, Slot C-1" 4 "13,113 x" 194 x TK TK and RW "1,934 mm^2" "5,418 mm^2" 31.5 µm^2 1.72% 1627 28 1100 39.3 ±12.10 ±2.33 1.8476E-02 2.8903E-07 64709 63240 97.7% " 1,469 " 38 38 30 37 29 28 57 49 27 44 37 38 36 28 28 53 50 duplicate 40 45 22 66 55 24 56 50 44 17 34 BALB/cBy please reshoot 1E-08 120594P BALB/cBy F 1 1 120594R "55,038" "±2,981" "55,038" "±2,981" 5.4% "42,200" 130.42 -0.582 ? 941205 30 14.5 408 1 1 "Very nice tissue. Lots of unmyelinated fibers, that were very easy to count." none Feb-8-95 18 "Box 38, Slot A-5" 4 "13,113 x" 193 x TK TK and RW "1,572 mm^2" "5,418 mm^2" 31.5 µm^2 1.57% 1339 21 863 41.1 ±9.95 ±2.23 6.5030E-03 1.1816E-07 58504 55838 95.4% " 2,666 " 28 39 43 52 41 41 34 60 48 53 45 31 53 20 39 50 28 32 37 43 46 BALB/cBy original F 1 1 120594S "60,984" "±2,202" "57,652" "±5,006" 8.7% "56,682" 101.71 0.791 ? 941205 30 14.8 404 1 1 1 necrotic Mar-1-95 19 "Box 38, Slot D-2" 4 "13,113 x" 194 x TK TK and RW "2,138 mm^2" "5,418 mm^2" 31.5 µm^2 1.17% 1799 21 673 32.0 ±12.44 ±2.78 1.3208E-02 2.1657E-07 64006 54992 85.9% " 9,014 " 0 39 57 20 38 32 40 43 47 42 20 27 35 17 22 34 31 25 30 30 44 BALB/cBy replicate 0.00001 120594S "61,784" "±2,452" 4.0% "55,403" 111.52 Replicate of a new section. none 3-May-95 19 "Box 38, Slot D-2" "12,950 x" 245 x RW RW and RW "3,333 mm^2" "5,270 mm^2" 31.4 µm^2 1.25% 1763 22 771 35.0 ±6.37 ±1.39 40 26 36 37 34 24 35 35 35 37 31 38 45 19 34 42 40 45 33 33 31 41 BALB/cBy original M 1 1 010695E "51,320" "±1,984" "49,245" "±3,449" 7.0% "87,773" 56.11 -1.441 ? 940807 950106 152 27.5 471 0 1 #12. Confirmed BALB/cBy. No problems of identification. Both CAST/Ei processed in same batch have low numbers. Beautiful tissue with large axons. none 5-Apr-95 9 "Box 42, Slot B-2" 4 "13,066 x" 190 x RW RW and RW "3,166 mm^2" "5,270 mm^2" 30.9 µm^2 0.77% 2844 22 381 17.3 ±5.56 ±1.21 1.3653E-02 2.6604E-07 20 19 23 27 23 13 21 17 14 9 23 26 21 5 19 11 15 14 15 18 12 16 BALB/cBy replicate 0.00001 010695E "52,347" "±2,426" 4.6% "82,258" 63.64 Replicate of different thin section 5-May-95 9 "Box 42, Slot B-2" "10,848 x" 206 x RW RW and RW "3,481 mm^2" "5,270 mm^2" 44.8 µm^2 1.20% 1837 22 627 28.5 ±6.05 ±1.32 14 29 29 38 25 38 36 30 33 25 30 23 21 33 20 23 34 31 28 33 26 28 BALB/cBy F 1 1 010695D "54,424" "±2,374" "54,424" "±2,374" 4.4% "82,356" 66.08 -0.724 ? 940807 950106 152 25.5 464 1 1 #11 Also cut beautiful tissue Apr-21-95 7 "Box 42, Slot A-1" 4 "12,802 x" 192 x RW RW and RW "3,052 mm^2" "5,270 mm^2" 32.2 µm^2 0.94% 2561 24 510 21.3 ±4.45 ±0.93 1.0077E-02 1.8516E-07 25 21 22 27 21 21 16 23 20 17 30 19 24 26 15 22 11 19 19 22 27 23 15 25 BALB/cBy bad block M 120594Q 941205 30 14.8 404 1 "First session of Feb 8, 95 no good. Section of nerve incomplete. Recut by Richelle, but still ragged." Feb-9-95 16 "Box 38, Slot B-4" TK BALB/cBy-mceph/+ M 5 071095A-2 463 941114 950710 238 NA 454 2 #1295 "N13, megaencephaly heterozygote from Cook. Unmapped locus." BALB/cBy-mceph/+ F 5 071095C-2 950409 950710 92 NA 468 1 #1334 N13F1. megaencephaly heterozygote BALB/cBy-mceph/+ F 5 071095E-2 950409 950710 92 NA 457 1 #1335 N13F1. megaencephaly heterozygote BALB/cBy-mceph/+ F 5 072595F-2 950505 950725 81 27.0 477 2 #1337 Megaencephaly heterozygote BALB/cBy-mceph/+ F 5 072595H-2 950507 950725 79 23.5 460 4 #1339 Megaencephaly heterozygote BALB/cBy-mceph/mceph F 5 071095B-2 545 950409 950710 92 NA 542 1 #1332 N13F1. megaencephaly homozygote BALB/cBy-mceph/mceph F 5 071095D-2 950409 950710 92 NA 550 1 #1333 N13F1. megaencephaly homozygote BALB/cBy-mceph/mceph F 5 072595E-2 "61,074" "±5,338" "61,074" "±5,338" 8.7% "108,741" 56.16 950505 950725 81 19.8 541 1 2 #1336 "Megaencephaly homozygote. Tissue is not pretty, but easy to count. Few unmyelinated fibers." none Sep-19-95 10 "Box 54, Slot A-1" 2 "12,872 x" 190 x RCS RCS and RW "3,945 mm^2" "5,324 mm^2" 32.1 µm^2 0.65% 3384 22 397 18.0 ±7.23 ±1.58 2.2456E-03 3.6769E-08 22 8 24 21 12 8 18 22 21 29 19 7 12 19 35 11 8 20 18 24 22 17 BALB/cBy-mceph/mceph F 5 072595G-2 950507 950725 79 20.0 549 3 #1338 Megaencephaly homozygote BALB/cBY.AKR congenic NM1360 M 5 062592E "40,395" "±2,336" "40,395" "±2,336" 5.8% "59,460" 67.94 Jackson New Mutant 1360 JAX1151 L 432 920423 920625 63 21.0 400 0 1 NM= new mutation at Jackson Laboratory from BALB/cBy.AKR cog stock. Mutant goes from side to side. N10F1 +13 All of these congenics and new mutants from the Jackson Lab Research colony. photo#22 is bad Aug-12-92 C2 "Box 16, Slot A-1" "13,158 x" 509 x LN 8-5-92 JO and JO "15,405 mm^2" "5,324 mm^2" 30.7 µm^2 1.40% 1934 27 564 20.9 ±6.16 ±1.21 7.6855E-03 1.9026E-07 40163 40610 101.1% 446 30 12 24 26 26 18 18 15 25 19 21 18 24 31 20 6 19 30 21 23 23 25 10 19 13 28 20 BALB/cBY.AKR-nm/nm F 081094A Jackson New Mutant 1360 JAX1154 930527 940810 440 23.0 408 2 Done at Jackson. An albino that looks sickly and old. Has a motor deficit. Died after injection. Perfused with 3.5% paraform with 0.1% glut. BALB/cBY.AKR-+/nm F 081094B Jackson New Mutant 1360 JAX1134 940330 940810 133 31.0 476 3 Done at JAX. Good perfusion. HRP injection. Normal heterozygote. BALB/cBY.AKR-+/nm F 081094C Jackson New Mutant 1360 JAX1135 940330 940810 133 31.0 457 3 Done at JAX. Good perfusion. HRP injection. Normal heterozygote. BALB/cBY.AKR-nm/nm M 081094D Jackson New Mutant 1360 JAX1136 940330 940810 133 23.0 418 3 "Done at JAX. Good perfusion. HRP injection. Mutant with smaller body, slow writhing response, normal pupillary light reflex." BALB/cBy.C57BL/6By-+ Hbb/+ Hbb F 5 062692C "69,555" "±6,204" "69,555" "±6,204" 8.9% "77,916" 89.27 JR0899 JAX525 ? 920627 100 19.0 434 1 "Brown agouti, BALB C.B6-+ Hbb/+ Hbb labeled Pigmented BALB/c. I don't trust the low calibration. It is way out of focus." 9-Mar-93 B2 "Box 20A, Slot D-2" 3 "12,857 x" 176 x DR DR and RW "2,414 mm^2" "5,313 mm^2" 32.1 µm^2 1.20% 2424 29 832 28.7 ±13.54 ±2.56 1.8716E-03 2.6907E-08 63898 74831 117.1% " 10,933 " 65 36 39 44 37 37 30 41 34 18 24 30 25 22 9 28 57 28 32 24 16 11 22 16 17 27 14 7 42 BALB/cJ F 1 1 112292C "63,393" "±2,210" "±5,846" 8 "68,149" "±3,016" "68,149" "±3,016" 4.4% "81,628" "3,310" 541 83.49 0.814 "54,683 " JR000651 L 477 920916 921123 68 477 13.9 1 1 Reshot montage and low mag calibration 1 necrotic axon on 0027 Jan-12-93 D3 "Box 21, Slot LÐ1" 4 "11,124 x" 250 x DR DR and RW "5,091 mm^2" "5,237 mm^2" 41.2 µm^2 1.66% 1983 33 1134 34.4 ±8.60 ±1.52 7.7283E-03 1.1340E-07 66684 65245 97.8% " 1,439 " 40 37 27 32 39 52 20 43 46 40 49 38 28 25 38 39 32 20 43 37 30 19 21 47 41 31 33 31 35 34 33 31 23 BALB/cJ F 1 1 112292D "67,477" "±3,169" "67,477" "±3,169" 4.7% "68,428" 98.61 0.699 "64,451 " JR000651 L 920916 921123 68 19.0 449 15.3 1 1 Great HRP backfill "1 or 2 necrotic axons in series, see 0041and 0015" Jan-12-93 C1 "Box 21, Slot E-1" 3 "13,347 x" 198 x DR DR and RW "2,685 mm^2" "5,237 mm^2" 28.9 µm^2 1.35% 2370 32 911 28.5 ±7.44 ±1.34 6.9355E-03 1.0278E-07 67105 67845 101.1% 741 20 34 37 20 26 29 15 33 22 35 23 21 34 37 32 22 27 10 32 31 28 31 34 26 34 31 36 15 25 39 33 39 BALB/cJ original M 1 1 011994E "63,529" "±2,441" "57,191" "±3,174" 5.5% "75,261" 75.99 0.023 JR000651 ? 940119 66 26.3 489 0 2 1 large necrotic axon 0009 Mar-31-94 1 "Box 26, Slot A-1" 3 "12,984 x" 194 x TK TK and RW "2,832 mm^2" "5,435 mm^2" 32.2 µm^2 1.03% 2334 24 588 24.5 ±6.52 ±1.36 1.1124E-02 1.7511E-07 58329 60347 103.5% " 2,018 " 20 30 27 26 36 32 24 28 18 31 29 15 20 22 34 30 21 18 27 16 31 18 12 23 BALB/cJ replicate 0.00001 011994E "60,295" "±3,819" 6.3% "74,556" 80.87 Replicate to confirm outlier status. Apr-17-95 1 "Box 26, Slot A-1" "13,096 x" 193 x RW RW and RW "2,782 mm^2" "5,270 mm^2" 30.7 µm^2 0.82% 2426 20 497 24.9 ±6.86 ±1.57 22 24 21 26 24 30 20 20 27 7 36 33 37 28 27 21 26 17 21 30 BALB/cJ other side 011994E "68,599" "±4,332" 6.3% "89,636" 76.53 Other side analyzed by Stacie none Jul-11-95 2 "Box 50, Slot A-1" 4 "12,877 x" 194 x SS SS and SS and RW "3,373 mm^2" "5,270 mm^2" 31.8 µm^2 1.21% 2820 34 827 24.3 ±8.82 ±1.54 10 20 13 21 33 31 28 28 29 29 16 21 26 8 18 21 17 52 19 25 26 30 16 13 19 28 23 19 30 41 31 28 34 24 BALB/cJ please reshoot this high side again 011994E BALB/cJ original M 1 1 011994F "53,252" "±2,743" "53,800" "±3,256" 6.1% "68,890" 78.10 -1.735 JR000651 R or L 940119 66 25.0 486 0 2 none Mar-31-94 3 "Box 26, Slot B-2" 3 "13,018 x" 194 x TK TK and RW "2,592 mm^2" "5,435 mm^2" 32.1 µm^2 1.02% 2148 22 551 25.0 ±6.95 ±1.52 7.4155E-03 1.3925E-07 55610 54609 98.2% " 1,000 " 26 37 35 27 25 14 25 21 20 28 13 22 24 25 18 34 25 19 41 25 26 21 BALB/cJ replicate 0.00001 011994F "52,927" "±5,092" 9.6% "70,498" 75.08 Redone by Toya to confirm low count 3 necrotic fibers Mar-6-95 3 "Box 26, Slot B-2" 4 "12,999 x" 193 x TK RW and RW "2,623 mm^2" "5,435 mm^2" 32.2 µm^2 0.91% 2192 20 483 24.2 ±10.13 ±2.32 46585 58068 124.7% " 11,484 " 24 17 23 27 27 18 11 26 19 44 14 37 15 21 9 29 39 12 34 37 BALB/cJ other side 011994F "52,958" "±4,135" 7.8% "70,548" 75.07 L or R Other side photographed by Stacie. none Jul-11-95 4 "Box 50, Slot B-4" 2 "12,852 x" 193 x SS SS and SS and RW "2,634 mm^2" "5,270 mm^2" 31.9 µm^2 0.95% 2211 21 503 24.0 ±8.36 ±1.87 30 26 28 24 1 35 25 27 31 20 18 32 36 23 22 28 16 20 28 25 8 BALB/cJ F 1 1 090894O "58,694" "±3,125" "58,694" "±3,125" 5.3% "60,807" 96.53 -0.804 JR000651 ? 940531 940908 100 22.0 446 "Brain weight is fine. Nerve switched with MOLD/Rk case 090894N. These micrographs are labeled 090894N, MOLD/Rk. Perfused by Richelle Strom. Examine other block to confirm switch. Reanalysis of other block pending." Low contrast negatives 1 necrotic on 0046 Nov-23-94 9 "Box 20, Slot K-2" 3 "12,906 x" 192 x THK RW and RW "2,237 mm^2" "5,418 mm^2" 32.5 µm^2 1.07% 1869 20 628 31.4 ±7.29 ±1.67 6.3277E-03 1.0781E-07 58235 56770 97.5% " 1,465 " 50 31 22 40 31 29 36 30 25 18 37 28 27 35 26 29 27 39 29 39 BALB/cJ original F 1 1 090894P "68,410" "±2,573" "69,253" "±3,254" 4.7% "75,927" 91.21 0.858 JR000651 ? 940908 100 24.0 491 1 3 Perfused by Richelle Strom 1 necrotic Dec-2-94 13 "Box 20, Slot P-1" 4 "12,993 x" 193 x TK RW and RW "2,821 mm^2" "5,418 mm^2" 32.1 µm^2 0.93% 2366 22 644 29.3 ±6.30 ±1.38 1.0826E-02 1.5824E-07 67552 71883 106.4% " 4,330 " 51 34 30 28 25 20 29 24 33 27 37 29 29 27 24 31 24 33 25 32 25 27 BALB/cJ replicate 0.00001 090894P "67,006" "±4,203" 6.3% "75,919" 88.26 Replicate to confirm outlier status. Apr-17-95 13 "Box 20, Slot P-1" "13,096 x" 193 x RW RW and RW "2,828 mm^2" "5,270 mm^2" 30.7 µm^2 1.01% 2471 25 678 27.1 ±8.33 ±1.70 17 27 32 21 17 21 27 33 25 12 35 32 26 26 42 24 30 35 28 42 30 37 31 11 17 BALB/cJ check sex F 1 1 090894Q "68,788" "±4,619" "68,788" "±4,619" 6.7% "87,868" 78.29 0.923 JR000651 ? 940908 100 NA 504 1 3 Perfused by Richelle Strom none Dec-2-94 16 "Box 20, Slot Q-4" 4 "13,037 x" 193 x TK RW and RW "3,260 mm^2" "5,418 mm^2" 31.9 µm^2 0.83% 2756 23 574 25.0 ±7.86 ±1.68 3.3713E-03 4.9010E-08 69365 69825 100.7% 459 32 20 27 26 21 24 16 31 12 29 28 31 42 15 30 30 38 22 15 30 23 14 18 BALB/cJ M 1 1 062192A "58,844" "±6,170" "58,844" "±6,170" 10.5% "58,112" 101.26 -0.778 JR000651 920621 200 27.0 NA 16.8 0 4 HRP backfill case. Retired breeder. Denny notes that mouse looks famished. This case was from the Research colony and JAX. Weird looking nerve full of unmyelinated fibers. Must have been cut close to the eye. Great wholemount. 2-4 necrotic Sep-19-94 C4 or C5 "Box 17, Slot L-1" 2 "16,200 x" 246 x RW RW and RW "3,527 mm^2" "5,418 mm^2" 20.6 µm^2 0.75% 2815 21 439 20.9 ±9.80 ±2.19 1.6230E-03 2.7582E-08 62036 71096 114.6% " 9,060 " 25 14 25 27 7 3 8 32 16 9 24 27 24 31 16 39 28 25 10 17 32 BALB/cJ perfuse Jan 95 M BALB/cJ perfuse Jan 95 F BALB/cJ perfuse Jan 95 F BXD1 F 1 2 012795O "61,996" "±1,077" "±3,231" 10 "60,772" "±3,637" "60,772" "±3,637" 6.0% "81,416" 74.64 -0.379 ? 448 941215 941215 42 14.3 405 1 1 F98. Bought from Jax. DBA color. "Maintained by Coleen Towser. All BXD strains in a single room at JAX. All but BXD13, BXD20, and BXD31, get the same Wayne Lab Tech High fish oil diet." 2 necrotic fibers Feb-19-95 7 "Box 38, Slot P-1" 4 "13,092 x" 194 x RCS RCS and RW "3,069 mm^2" "5,418 mm^2" 31.6 µm^2 1.24% 2576 32 755 23.6 ±7.86 ±1.41 4.7432E-03 7.8050E-08 61011 60528 99.2% 483 15 23 14 17 32 29 7 17 21 19 14 33 36 23 37 19 32 19 25 13 13 32 31 27 30 37 18 22 25 23 26 26 BXD1 M 1 2 012795N "59,552" "±3,865" "59,552" "±3,865" 6.5% "83,233" 71.55 -0.756 ? 941217 941217 42 18.0 448 0 1 F98. Bought from Jax. DBA color. none Feb-19-95 5 "Box 38, Slot O-2" 4 "13,092 x" 194 x RCS RCS and RW "3,138 mm^2" "5,418 mm^2" 31.6 µm^2 0.99% 2633 26 588 22.6 ±7.34 ±1.47 4.1470E-03 6.9636E-08 58132 60968 104.9% " 2,836 " 30 20 6 20 21 3 23 20 21 27 26 35 20 23 23 36 21 14 30 22 30 19 22 23 28 25 BXD1 original F 1 2 021795F "55,714" "±3,022" 5.4% "69,786" 79.84 -19.186 ? 941226 950217 53 16.5 432 1 2 or 3 F97. Bought from Jax. "Marginal fixation. Low count probably due to failure to count unmyelinated axons. Cases F through K are a mix from two litters born 12/25 and 12/27, 1994. Both litters are F97. DBA coloration." none Apr-5-95 15 "Box 41, Slot N-1" 2 "13,092 x" 195 x RW RW and RW "2,663 mm^2" "5,270 mm^2" 30.7 µm^2 0.97% 2270 22 540 24.5 ±6.10 ±1.33 #DIV/0! #DIV/0! 34 29 22 29 21 33 29 30 20 8 20 22 26 24 19 24 24 35 24 23 26 18 BXD1 replicate 0.00001 021795F "58,806" "±4,650" 7.9% "74,125" 79.33 Marginal fixation. Unmyelinated axons difficult to resolve. possibly 1 necrotic axon Jan-22-96 15 "Box 41, Slot N-4" 2 "13,031 x" 185 x RW RW and RW "2,546 mm^2" "5,263 mm^2" 31.0 µm^2 0.92% 2391 22 541 24.6 ±8.91 ±1.94 23 36 14 38 36 27 26 12 28 37 25 28 18 5 25 30 25 25 13 34 19 17 BXD1 F 1 2 021795G "60,365" "±4,636" "60,365" "±4,636" 7.7% "92,376" 65.35 -0.505 ? 941226 950217 53 15.5 417 1 2 or 3 F97. Bought from Jax. "Cases F through K are a mix from two litters born 12/25 and 12/27, 1994. Both litters are F97. DBA coloration." none Apr-5-95 18 "Box 41, Slot O-2" 4 "13,092 x" 195 x RW RW and RW "3,524 mm^2" "5,270 mm^2" 30.7 µm^2 0.73% 3005 22 442 20.1 ±7.07 ±1.54 2.9427E-03 4.8749E-08 17 30 14 10 13 21 13 20 17 9 24 19 32 20 28 28 26 19 34 15 15 18 BXD1 could replicate M 1 2 021795H "67,167" "±5,996" "67,167" "±5,996" 8.9% "102,264" 65.68 1.600 ? 941226 950217 53 23.0 466 0 2 or 3 "F97. Cases F through K are a mix from two litters born 12/25 and 12/27, 1994. Both litters are F97. DBA coloration." Some lovely huge axons. none Apr-20-95 20 "Box 41, Slot P-1" 4 "13,074 x" 193 x RCS RW and RW "3,800 mm^2" "5,270 mm^2" 30.8 µm^2 0.60% 3317 20 405 20.3 ±7.88 ±1.81 1.9665E-03 2.9278E-08 37 31 28 26 11 24 12 23 16 18 27 28 13 8 21 duplicate 10 13 16 23 20 BXD1 M 1 2 021795I "67,150" "±5,342" "67,150" "±5,342" 8.0% "95,738" 70.14 1.595 ? 941226 950217 53 24.5 465 0 2 or 3 F97. Bought from Jax. "Cases F through K are a mix from two litters born 12/25 and 12/27, 1994. Both litters are F97. DBA coloration." none Apr-20-95 21 "Box 41, Slot R-1" 4 "12,933 x" 193 x RCS RW and RW "3,557 mm^2" "5,270 mm^2" 31.5 µm^2 0.66% 3038 20 442 22.1 ±7.66 ±1.76 2.4771E-03 3.6889E-08 26 23 18 26 32 23 20 20 15 27 19 40 27 5 11 29 15 25 19 22 BXD1 M 1 2 021795J "60,419" "±5,782" "60,419" "±5,782" 9.6% "77,454" 78.01 -0.488 ? 941226 950217 53 24.6 467 0 2 or 3 F97. Bought from Jax. "Cases F through K are a mix from two litters born 12/25 and 12/27, 1994. Both litters are F97. DBA coloration." 1 necrotic Apr-20-95 2 "Box 41, Slot S-2" 4 "12,933 x" 193 x RCS RW and RW "2,878 mm^2" "5,270 mm^2" 31.5 µm^2 0.77% 2458 19 467 24.6 ±9.98 ±2.35 1.9076E-03 3.1573E-08 37 12 23 30 37 28 22 28 5 15 33 24 23 33 23 14 15 45 20 BXD1 could replicate M 1 2 021795K "61,185" "±6,648" "61,185" "±6,648" 10.9% "95,914" 63.79 -0.251 ? 941226 950217 53 18.5 435 0 2 or 3 F97. Bought from Jax. "Cases F through K are a mix from two litters born 12/25 and 12/27, 1994. Both litters are F97. DBA coloration." 1 necrotic Apr-20-95 5 "Box 41, Slot Q-2" 4 "12,933 x" 193 x RCS RW and RW "3,564 mm^2" "5,270 mm^2" 31.5 µm^2 0.66% 3044 20 402 20.1 ±9.52 ±2.18 1.4573E-03 2.3818E-08 36 26 11 6 12 15 32 13 32 19 28 14 13 17 22 8 33 29 27 9 BXD1 M 1 2 071795A-2 "64,458" "±5,431" "64,458" "±5,431" 8.4% "102,054" 63.16 0.762 ? 950526 950717 52 24.0 439 0 4 F99/98. Fixed at JAX by Dan. none Dec-20-95 9 "Box 56, Slot N-1" 3 "12,853 x" 191 x RCS RW and RW "3,734 mm^2" "5,270 mm^2" 31.9 µm^2 0.63% 3199 20 403 20.2 ±7.40 ±1.70 2.3004E-03 3.5688E-08 29 18 17 28 25 12 3 35 28 20 23 15 17 23 24 17 17 9 20 23 BXD1 original M 1 2 071795B-2 "57,404" "±4,260" "57,404" "±4,260" 7.4% "88,694" 64.72 -1.421 ? 950526 950717 52 23.5 482 0 4 F99/98. Fixed at JAX by Dan. none Jan-5-96 10 "Box 56, Slot O-2" 4 "13,066 x" 190 x RW RW and RW "3,209 mm^2" "5,263 mm^2" 30.8 µm^2 0.73% 2877 21 419 20.0 ±6.62 ±1.48 3.3214E-03 5.7859E-08 14 26 20 35 18 19 25 25 21 16 9 23 22 8 16 20 18 24 17 31 12 BXD1 replicate M 071795B-2 "51,460" "±4,558" 8.9% "79,721" 64.55 ? Repeat by Rob of different section. Nice tissue and good contrast. none Feb-1-96 10 "Box 56, Slot O-2" 4 "13,066 x" 190 x RW RW and RW "2,884 mm^2" "5,396 mm^2" 31.6 µm^2 0.79% 2523 20 408 20.4 ±7.88 ±1.81 #DIV/0! #DIV/0! 23 1 22 16 30 20 23 25 27 17 18 25 24 22 9 30 31 13 8 24 BXD1 M 1 2 071795C-2 "61,492" "±4,398" "61,492" "±4,398" 7.2% "79,001" 77.84 -0.156 ? 950526 950717 52 22.0 461 0 4 F99/98. Fixed at JAX by Dan. 2 necrotic Jan-5-96 12 "Box 56, Slot P-1" 4 "13,006 x" 191 x RW RW and RW "2,886 mm^2" "5,263 mm^2" 31.1 µm^2 0.91% 2539 23 557 24.2 ±8.12 ±1.73 3.3239E-03 5.4054E-08 34 12 27 22 17 22 23 29 31 28 20 6 18 29 23 26 28 28 37 42 15 22 18 BXD1 check status M 071795D-2 ? 950526 950717 52 21.2 461 4 F99/98. Fixed at JAX by Dan. BXD2 original F 1 2 012795E "66,063" "±1,644" "±3,677" 6 "60,417" "±3,708" "61,761" "±5,290" 8.6% "87,143" 70.87 -1.536 ? 427 941216 950127 42 16.8 415 1 1 F123 or F119. Bought from Jax. B.Taylor: BXD2 has hydrocephaly none Feb-15-95 9 "Box 38, Slot F-2" 4 "13,078 x" 182 x TK RW and RW "2,901 mm^2" "5,418 mm^2" 31.7 µm^2 0.73% 2751 20 449 22.5 ±8.38 ±1.92 4.6260E-03 7.6568E-08 64923 54194 83.5% " 10,729 " 16 41 14 11 11 16 22 26 36 27 21 32 14 17 22 21 21 23 23 35 BXD2 replicate F 0.00001 012795E "59,119" "±5,198" 8.8% "85,457" 69.18 "Replicate analysis on different thin section. RW photo, RW count, RW calibrate" none Mar-10-95 9 "Box 38, Slot F-2" "13,081 x" 192 x RW RW and RW "3,158 mm^2" "5,418 mm^2" 31.7 µm^2 0.78% 2699 21 460 21.9 ±8.61 ±1.93 30 26 2 10 28 28 34 32 22 30 24 15 14 26 24 26 20 17 25 5 22 BXD2 original F 1 2 012795F "63,995" "±3,086" "69,642" "±4,347" 6.2% "71,993" 96.74 -0.563 ? 941216 950127 42 15.8 409 1 1 F123 or F119. Bought from Jax. B.Taylor: BXD2 has hydrocephaly none Feb-23-95 12 "Box 38, Slot M-4" 3 "13,137 x" 194 x TK TK and TK and RW "2,713 mm^2" "5,418 mm^2" 31.4 µm^2 0.83% 2293 19 577 30.4 ±8.04 ±1.90 7.0936E-03 1.1085E-07 69592 70756 101.7% " 1,164 " 38 48 20 38 40 28 25 27 42 23 25 30 27 24 23 30 26 23 40 BXD2 replicate F 0.00001 012795F "58,259" "±4,381" 7.5% "70,414" 82.74 "Replicate analysis on different thin section. RW photo, RW count, RW calibrate" none Mar-10-95 12 "Box 38, Slot M-4" "13,031 x" 192 x RW RW and RW "2,602 mm^2" "5,418 mm^2" 31.9 µm^2 1.13% 2207 25 660 26.4 ±9.73 ±1.99 39 18 25 39 31 32 26 29 38 24 29 16 28 42 30 32 2 22 16 4 31 25 24 27 31 BXD2 original M 1 2 012795G "65,062" "±3,592" "72,193" "±6,441" 8.9% "102,120" 70.69 -0.272 ? 941211 950127 47 26.5 425 0 2 F120 or F121. Bought from Jax. none Feb-16-95 14 "Box 38, Slot I-5" 3 "12,905 x" 193 x TK RW and RW "3,792 mm^2" "5,418 mm^2" 32.5 µm^2 0.64% 3139 20 460 23.0 ±8.94 ±2.05 5.3094E-03 8.1605E-08 69993 70307 100.4% 314 15 18 20 22 25 27 34 33 24 22 35 22 12 35 36 26 13 2 16 23 BXD2 replicate M 0.00001 012795G "61,844" "±4,327" 7.0% "105,832" 58.44 "Replicate count on different thin section. RW photo, RW count, RW calibrate" none Mar-10-95 14 "Box 38, Slot I-1" "12,985 x" 192 x RW RW and RW "3,911 mm^2" "5,418 mm^2" 32.1 µm^2 0.82% 3293 27 507 18.8 ±6.70 ±1.31 11 14 11 15 16 23 16 28 28 18 15 17 12 15 23 20 16 10 25 19 29 27 10 21 13 19 36 BXD2 original M 1 2 012795H "67,730" "±2,473" "65,997" "±3,876" 5.9% "80,007" 82.49 0.453 ? 941211 950127 47 27.5 427 0 2 F120 or F121. Bought from Jax. Low mag is correctly calibrated. B.Taylor: BXD2 has hydrocephaly none Feb-15-95 15 "Box 38, Slot H-2" 3 "13,098 x" 182 x TK RW and RW "2,663 mm^2" "5,418 mm^2" 31.6 µm^2 0.79% 2533 20 521 26.1 ±6.67 ±1.53 1.1659E-02 1.7213E-07 65408 58775 89.9% " 6,633 " 27 26 28 32 23 37 34 19 25 22 23 27 28 35 14 21 not in nerve 16 30 18 36 BXD2 replicate M 0.00001 012795H "68,920" "±3,211" 4.7% "78,152" 88.19 "Replicate analysis of different thin section. RW photo, RW count, RW calibrate" 1 necrotic Mar-10-95 15 "Box 38, Slot H-2" "13,084 x" 192 x RW RW and RW "2,884 mm^2" "5,418 mm^2" 31.6 µm^2 0.89% 2469 22 614 27.9 ±5.96 ±1.30 36 29 20 30 28 30 23 32 32 32 33 25 21 32 33 34 31 23 30 11 21 28 BXD2 original M 1 2 012795I "70,774" "±3,988" "70,168" "±5,586" 8.0% "106,415" 65.94 1.281 ? 941117 950127 71 28.5 461 0 3 F120 or F121. Bought from Jax. B.Taylor: BXD2 has hydrocephaly 1 necrotic Feb-23-95 17 "Box 38, Slot N-1" 3 "13,137 x" 194 x TK TK and TK and RW "4,010 mm^2" "5,418 mm^2" 31.4 µm^2 0.59% 3390 20 414 20.7 ±7.18 ±1.65 4.6840E-03 6.6182E-08 68737 76177 110.8% " 7,441 " 8 22 24 12 16 7 19 35 27 29 18 17 26 25 25 22 24 13 27 18 BXD2 replicate M 0.00001 012795I "71,405" "±5,696" 8.0% "105,099" 67.94 "Replicate on different thin section. RW photo, RW count, RW calibrate" none Mar-10-95 17 "Box 38, Slot N-3" "13,028 x" 194 x RW RW and RW "3,949 mm^2" "5,418 mm^2" 31.9 µm^2 0.49% 3292 16 347 21.7 ±6.70 ±1.73 25 22 8 10 19 16 24 21 27 36 double exposure 19 21 22 26 27 24 BXD2 original M 1 2 012795J "68,402" "±3,395" "70,414" "±4,259" 6.0% "88,470" 79.59 0.636 ? 941117 950127 71 23.5 422 0 3 F120 or F121. Bought from Jax. B.Taylor: BXD2 has hydrocephaly none Feb-21-95 20 "Box 38, Slot K-2" 3 "13,086 x" 194 x TK TK and RW "3,315 mm^2" "5,418 mm^2" 31.6 µm^2 0.79% 2796 22 554 25.2 ±6.98 ±1.52 6.2158E-03 9.0871E-08 67107 73971 110.2% " 6,863 " 19 22 15 24 37 26 32 17 22 30 27 25 26 25 41 26 28 12 27 34 20 19 BXD2 replicate M 0.00001 012795J "64,895" "±5,624" 8.7% "89,538" 72.48 "Replicate count on different thin section. RW photo, RW count, RW calibrate" none Mar-10-95 20 "Box 38, Slot K-2" "13,084 x" 192 x RW RW and RW "3,299 mm^2" "5,418 mm^2" 31.6 µm^2 0.57% 2829 16 367 22.9 ±7.70 ±1.99 9 31 22 31 29 20 22 36 28 25 21 18 12 11 28 24 BXD5 M 1 2 112894A "75,548" "±1,273" "±2,846" 6 "73,258" "±3,720" "73,258" "±3,720" 5.1% "92,051" 79.58 -0.804 ? 505 941001 941128 58 20.3 509 478 51 0 1 F86 bought from JAX. Gray coat none Feb-2-95 2 "Box 36, Slot P-3" 4 "13,074 x" 194 x TK RW and TK "3,476 mm^2" "5,418 mm^2" 31.7 µm^2 0.76% 2904 22 555 25.2 ±5.87 ±1.28 5.5446E-03 7.5685E-08 76028 70484 92.7% " 5,544 " 30 27 23 19 20 27 19 28 23 16 37 29 17 36 21 27 21 24 22 30 34 25 BXD5 F 1 2 112894B "77,290" "±4,928" "77,290" "±4,928" 6.4% "112,128" 68.93 0.612 ? 940921 941128 68 17.5 504 483 51 1 2 F86 bought from JAX. Gray coat none Feb-2-95 3 "Box 36, Slot Q-4" 3 "13,074 x" 194 x TK RW and TK "4,234 mm^2" "5,418 mm^2" 31.7 µm^2 0.57% 3537 20 437 21.9 ±6.07 ±1.39 3.3506E-03 4.3351E-08 76757 77818 101.4% " 1,061 " 26 24 29 11 28 13 20 22 21 23 22 22 16 31 30 25 17 18 11 28 BXD5 brain to Allman M 1 2 011795M "76,652" "±6,485" "76,652" "±6,485" 8.5% "98,296" 77.98 0.388 ? 941201 950117 47 19.8 521 504 52 3 GET none Mar-30-95 17 "Box 41, Slot F-1" 4 "13,086 x" 193 x RCS RW and RW "3,665 mm^2" "5,270 mm^2" 30.8 µm^2 0.59% 3194 19 456 24.0 ±8.62 ±2.03 1.9236E-03 2.5096E-08 19 4 30 25 13 17 32 23 26 24 36 25 40 24 28 30 11 22 27 BXD5 M 1 2 013095O "72,566" "±7,023" "72,566" "±7,023" 9.7% "104,982" 69.12 -1.047 ? 941201 950130 60 20.8 518 516 53 0 3 F86. Bought from Jax. Grey coat none Mar-2-95 18 "Box 40, Slot I-2" 4 "13,061 x" 194 x RCS RW and RW "3,939 mm^2" "5,418 mm^2" 31.8 µm^2 0.67% 3305 22 483 22.0 ±9.74 ±2.12 1.5413E-03 2.1240E-08 64301 80827 125.7% " 16,526 " 21 16 16 7 17 34 29 17 6 30 17 36 35 20 14 34 17 9 18 39 24 27 BXD5 F 1 2 013095P "73,663" "±4,575" "73,663" "±4,575" 6.2% "98,719" 74.62 -0.662 ? 941220 950130 41 13.3 472 471 50 1 4 F86. Bought from Jax. Grey coat none Mar-2-95 21 "Box 40, Slot K-4" 4 "13,061 x" 194 x RCS RCS and RW "3,704 mm^2" "5,418 mm^2" 31.8 µm^2 0.64% 3108 20 474 23.7 ±6.42 ±1.47 3.7044E-03 5.0288E-08 77079 70242 91.1% " 6,838 " 15 16 31 38 27 19 35 26 25 15 24 21 19 22 29 27 21 16 22 26 BXD5 brain to Allman F 1 2 013095Q "79,857" "±3,890" "79,857" "±3,890" 4.9% "85,019" 93.93 1.514 ? 941220 950130 41 15.0 503 506 48 1 4 F86. Bought from Jax. Grey coat 1 necrotic Mar-2-95 1 "Box 40, Slot J-4" 4 "13,061 x" 194 x RCS RW and RW "3,190 mm^2" "5,418 mm^2" 31.8 µm^2 0.67% 2677 18 537 29.8 ±5.99 ±1.45 5.5863E-03 6.9954E-08 78814 80896 102.6% " 2,082 " 35 34 30 31 31 39 30 26 41 26 27 30 19 17 30 27 29 35 BXD5 "arrived 2/21/95, use to make F1 hybrids" M 121995P ? 950110 951219 343 31.5 540 4 "F86. Bought from Jax, from rm A-1." "Light grey coat. Arrived 2/21/95, use to make F1 hybrids. Breeder #228" BXD5 "arrived July 13, 1995" F 121995Q 950528 951219 205 22.5 545 5 F88/87 Grey coat. Breeder #346 BXD5 "arrived July 13, 1995" F breeder 950528 5 F88/87 Grey coat BXD6 original F 1 2 073093D "62,695" ±801 "±1,961" 7 "64,509" "±3,492" "63,957" "±4,473" 7.0% "70,025" 91.33 0.925 ? 390 930701 930730 29 NA NA NA 1 1 F114/118. Light stain. Mouse from Ben Taylor's colony. Only 1 possibly necrotic axon noted Jan-4-93 B1 "Box 24, Slot C-1" 2 "13,120 x" 239 x TK TK and RW and RW "4,012 mm^2" "5,381 mm^2" 31.3 µm^2 0.89% 2240 20 571 28.6 ±8.70 ±2.00 5.5683E-03 8.6317E-08 68897 64009 92.9% " 4,888 " To far right 27 33 15 24 23 31 35 40 29 31 19 28 17 21 50 33 28 17 32 38 BXD6 replicate 0.00001 073093D "±1,001" "65,372" "±5,589" 8.5% "77,415" 84.44 Replicate analysis of different thin section. Mar-13-94 B1 "Box 24, Slot C-3" "13,199 x" 193 x RCS RCS and RW "2,893 mm^2" "5,418 mm^2" 31.1 µm^2 0.76% 2489 19 499 26.3 ±9.53 ±2.25 15 33 26 26 29 27 34 8 27 30 46 35 19 33 25 29 31 20 6 BXD6 original M 1 2 081194C "60,074" "±2,861" "66,617" "±4,941" 7.4% "94,462" 70.52 -1.337 ? 940214 940811 178 384 384 40 0 2 "F120 Retired breeder from Ben Taylor's colony. Good pupillary light reflex. Agouti, steel grey." Brainstem missingÑnot found. none Oct-5-94 15 "Box 28, Slot R-1" 3 "13,046 x" 248 x RC RC and RW "5,815 mm^2" "5,418 mm^2" 31.8 µm^2 0.67% 2967 20 449 22.5 ±7.26 ±1.67 7.7268E-03 1.2862E-07 21 21 28 18 7 28 33 21 19 22 23 31 8 27 17 25 14 29 32 25 BXD6 replicate 0.00001 081194C "±6,959" "56,775" "±3,509" 6.2% "100,697" 56.38 "Replicate analysis of different thin section. Rob did calibrations, not Toya." none Mar-11-95 15 "Box 36, Slot R-3" "13,044 x" 192 x RW DR and DR "3,700 mm^2" "5,418 mm^2" 31.8 µm^2 0.70% 3162 22 395 18.0 ±5.08 ±1.11 24 24 25 18 21 24 12 17 17 15 16 19 12 24 17 21 22 8 17 7 16 19 BXD6 original M 1 2 080994A "63,617" "±3,708" "62,737" "±6,233" 9.9% "85,946" 73.00 0.470 ? 940128 940809 193 384 392 43 0 3 "F119 Retired breeder from Ben Taylor's colony. Good pupillary light reflex. Agouti, steel grey." Cut by Richelle. Please check nerve area and make note. none Sep-16-94 16 "Box 28, Slot C-1" 3 "12,942 x" 195 x RCS RCS and RW "3,264 mm^2" "5,418 mm^2" 32.3 µm^2 0.68% 2657 18 425 23.6 ±9.67 ±2.35 4.8997E-03 7.7019E-08 29 36 25 24 13 22 11 38 25 24 21 18 9 17 46 31 16 20 BXD6 replicate 0.00001 080994A ±963 "64,099" "±4,613" 7.2% "90,450" 70.87 Replicate analysis of different thin section. none Mar-12-95 16 "Box 28, Slot C-3" "13,120 x" 194 x DR TK and RCS "3,399 mm^2" "5,418 mm^2" 31.5 µm^2 0.80% 2874 23 513 22.3 ±7.53 ±1.61 34 11 25 20 35 13 29 30 12 24 28 25 19 21 15 19 33 11 24 24 24 11 26 BXD6 original F 1 2 080994B "64,712" "±2,655" "64,934" "±4,295" 6.6% "97,146" 66.84 1.029 ? 940128 940809 193 397 405 45 1 3 "F119 Retired breeder from Ben Taylor's colony. Good pupillary light reflex. Agouti, steel grey. Fixation of unmyelinated fibers is poor. Count is fine though." Cut by Richelle. none Sep-20-94 19 "Box 28, Slot D-2" 3 "13,080 x" 194 x RCS RW and RW "3,645 mm^2" "5,418 mm^2" 31.7 µm^2 0.59% 3068 18 381 21.2 ±5.77 ±1.40 9.7209E-03 1.5022E-07 27 21 20 31 25 27 26 19 24 15 17 11 18 19 28 20 23 10 BXD6 replicate 0.00001 080994B ±254 "64,574" "±3,377" 5.2% "101,984" 63.32 Replicate analysis of the same section used in the original analysis. none Mar-12-95 19 "Box 28, Slot D-2" "13,004 x" 193 x DR TK and RCS "3,800 mm^2" "5,418 mm^2" 32.0 µm^2 0.66% 3183 21 426 20.3 ±4.74 ±1.06 22 16 16 18 19 22 27 22 21 21 12 12 20 20 16 25 16 19 27 26 29 BXD6 original M 1 2 112894C "63,138" "±2,474" "64,328" "±3,189" 5.0% "66,421" 96.85 0.226 ? 941001 941128 58 19.8 388 372 40 0 4 F100+. Bought from Jax. Gray coat none Feb-2-95 5 "Box 36, Slot R-3" 3 "13,074 x" 194 x TK RW and RW "2,508 mm^2" "5,418 mm^2" 31.7 µm^2 0.95% 2095 20 614 30.7 ±6.63 ±1.52 1.0863E-02 1.7205E-07 32 38 36 27 26 28 19 34 27 41 28 29 28 21 39 43 25 29 38 26 BXD6 replicate 0.00001 112894C "±2,113" "61,340" "±3,919" 6.4% "67,507" 90.86 Replicate analysis of different thin section. none Mar-11-95 5 "Box 36, Slot R-3" "13,029 x" 194 x RCS RW and RW "2,547 mm^2" "5,418 mm^2" 31.9 µm^2 0.85% 2115 18 522 29.0 ±7.64 ±1.85 33 31 32 21 40 24 35 24 36 29 28 40 37 33 28 15 19 17 BXD6 original M 1 2 112894D "59,911" "±2,833" "62,998" "±4,681" 7.4% "74,885" 84.13 -1.420 ? 941001 941128 58 20.0 393 376 42 0 4 F100+. Bought from Jax. Gray coat none Feb-2-95 8 "Box 36, Slot S-2" 3 "13,074 x" 194 x TK TK and RW "2,823 mm^2" "5,418 mm^2" 31.7 µm^2 0.89% 2362 21 560 26.7 ±8.86 ±1.98 7.8405E-03 1.3087E-07 23 38 10 20 29 33 25 35 32 11 34 32 27 32 16 27 25 35 36 9 31 BXD6 replicate 0.00001 112894D "±3,443" "58,129" "±3,558" 6.1% "76,883" 75.61 Replicate analysis of different thin section. none Mar-11-95 8 "Box 36, Slot S-2" "13,002 x" 193 x RCS DR and RCS "2,878 mm^2" "5,418 mm^2" 32.0 µm^2 1.08% 2399 26 630 24.2 ±7.42 ±1.48 25 31 19 29 36 21 24 25 25 22 34 32 30 9 17 25 27 22 17 31 24 36 27 7 19 16 BXD6 original F 1 2 112894E "62,904" "±1,919" "65,079" "±2,571" 4.0% "74,067" 87.87 0.107 ? 940905 941128 84 19.5 382 362 40 1 5 F123. Bought from Jax. Gray coat none Feb-8-95 9 "Box 36, Slot T-1" 3 "13,098 x" 193 x TK TK and RW "2,773 mm^2" "5,418 mm^2" 31.6 µm^2 0.85% 2345 20 555 27.8 ±4.78 ±1.10 1.7984E-02 2.8590E-07 25 30 27 24 19 26 21 31 25 33 37 30 28 25 27 20 30 30 34 33 BXD6 replicate 0.00001 112894E "±3,473" "60,168" "±2,883" 4.8% "77,999" 77.14 Replicate analysis of different thin section. none Mar-11-95 9 "Box 36, Slot T-1" "12,990 x" 193 x RCS DR and RCS "2,898 mm^2" "5,418 mm^2" 32.1 µm^2 1.07% 2429 26 644 24.8 ±5.94 ±1.19 24 18 14 26 23 26 23 24 21 23 33 10 26 32 22 31 31 22 25 23 27 28 20 35 35 22 BXD6 poor fix F 081194D "109,856" 0.00 ? 940214 940811 178 400 407 46 2 "F120 Retired breeder from Ben Taylor's colony. Good pupillary light reflex. Agouti, steel grey." Fix is poor and the quality of the axons is quite poor 1 necrotic Oct-5-94 17 "Box 28, Slot S-1" 1 "13,044 x" 248 x RC RC and RW "6,763 mm^2" "5,418 mm^2" 31.8 µm^2 0.58% 3450 20 314 15.7 ±5.79 ±1.33 16 18 5 19 23 25 11 15 6 15 15 19 16 17 21 14 11 10 27 11 BXD6 poor fix F 073093B "99,309" 0.00 ? 930701 930730 29 NA NA NA 1 F114/118. Terrible fixation at JAX. Cannot reliably count unmyelinated axons. Mouse from Ben Taylor's colony. Jan-4-93 A1 "Box 24, Slot A-1" 1 "12,988 x" 244 x TK TK and RW "5,930 mm^2" "5,352 mm^2" 31.7 µm^2 0.80% 3130 25 435 17.4 ±6.00 ±1.22 17 21 15 22 25 24 16 21 23 13 6 10 15 12 20 13 16 35 19 11 14 20 14 13 20 BXD6 poor fix F 073093C "76,419" 0.00 ? 930701 930730 29 NA NA NA 1 F114/118. Terrible fixation at JAX. Cannot reliably count unmyelinated axons. Mouse from Ben Taylor's colony. none Dec-23-93 A4 "Box 24, Slot B-2" 1 "13,046 x" 243 x TK TK and RW "4,523 mm^2" "5,345 mm^2" 31.4 µm^2 0.90% 2433 22 475 21.6 ±7.50 ±1.64 21 23 18 36 24 18 23 3 26 27 30 20 30 30 6 16 18 23 25 19 22 17 BXD6 aged mouse F 072994B "51,844" "±3,341" 6.4% "109,917" 47.17 ? 920628 940729 761 410 410 49 6 Aged (P500) BXD from Ben Taylor's colony. High quality tissue (excellent=4) Lots of necrosis Oct-15-94 3 "Box 29, Slot Q-2" "13,033 x" 193 x RCS RCS and RW "4,113 mm^2" "5,418 mm^2" 31.9 µm^2 0.67% 3446 23 346 15.0 ±4.55 ±0.97 12 13 18 19 19 13 10 16 6 25 13 19 18 22 6 14 15 16 13 14 13 13 19 BXD8 F 1 2 073093A "60,153" "±1,433" "±2,026" 3 "60,910" "±5,049" "60,910" "±5,049" 8.3% "104,462" 58.31 0.374 ? 430 930619 930730 41 NA 1 1 F90. Mouse from Ben Taylor's colony Perfused at JAX by Dan. Poor fixation. Hard to count unmyelinated. May be low. none Jan-18-94 D4 "Box 24, Slot F-2" 2 "13,012 x" 249 x TK TK and RW "6,501 mm^2" "5,343 mm^2" 31.6 µm^2 0.76% 3310 25 460 18.4 ±7.47 ±1.53 2.4888E-03 4.0860E-08 60961 57786 94.8% " 3,175 " 16 11 25 17 21 15 25 23 17 25 5 29 11 22 26 30 More data to right 23 4 18 28 10 20 7 19 13 BXD8 F 1 2 112894F "61,691" "±3,982" "61,691" "±3,982" 6.5% "84,611" 72.91 0.759 ? 940902 941128 87 19.8 423 1 5 F94 bought from JAX. The high mag calibration image is somewhat distorted. none Feb-8-95 12 "Box 38, Slot E-1" 4 "12,655 x" 193 x TK TK and RW "3,153 mm^2" "5,418 mm^2" 33.8 µm^2 0.96% 2501 24 592 24.7 ±7.64 ±1.59 4.0589E-03 6.5794E-08 66275 66275 100.0% 40 30 28 12 23 24 12 19 24 29 23 18 35 22 20 33 23 23 18 16 24 28 26 42 BXD8 M 1 2 080393E "57,856" "±4,095" "57,856" "±4,095" 7.1% "82,147" 70.43 -1.133 ? 930630 930803 34 NA 0 2 F89. Good stain. Mouse from Ben Taylor's colony. Perfused at JAX by DAN none Jan-10-94 B6 "Box 24, Slot E-1" 3 "12,889 x" 246 x TK TK and RW "4,955 mm^2" "5,353 mm^2" 32.2 µm^2 0.90% 2549 23 522 22.7 ±7.53 ±1.61 3.6072E-03 6.2347E-08 67850 54133 79.8% " 13,717 " 20 28 31 27 22 12 17 26 18 24 14 32 8 25 17 18 22 24 21 41 35 20 20 BXD8 M 080994C "60,565" "±4,904" 8.1% "136,777" 44.28 -29.685 ? 931111 940809 271 429 0 3 "F91 retired breeder from Ben Taylor's colony. Agouti, steel grey." Nice large axons. Light stain. none Aug-20-94 21 "Box 28, Slot F-2" 3 "13,056 x" 194 x RCS RW and RCS "5,161 mm^2" "5,418 mm^2" 31.8 µm^2 0.63% 4303 27 380 14.1 ±5.81 ±1.14 #DIV/0! #DIV/0! 74374 53571 72.0% " 20,803 " 25 15 17 9 8 20 9 18 7 19 4 14 8 20 15 24 10 15 12 18 9 22 12 10 22 10 8 BXD8 original F 080994D "54,311" "±3,278" 6.0% "97,830" 55.52 -29.685 ? 931111 940809 271 430 1 3 "F91 retired breeder from Ben Taylor's colony. Agouti, steel grey." Cut by Richelle. Sample is somewhat biased to edge. 3 necrotic fibers Sep-16-94 1 "Box 28, Slot B-2" 4 "12,978 x" 194 x RCS RCS and RW "3,666 mm^2" "5,418 mm^2" 32.2 µm^2 0.69% 3041 21 375 17.9 ±4.82 ±1.08 #DIV/0! #DIV/0! 63649 55266 86.8% " 8,383 " 17 17 17 21 9 17 17 18 10 20 22 11 edge 19 11 17 24 19 18 28 25 18 BXD8 replicate 0.00001 "50,360" "±3,168" 6.3% "102,884" 48.95 I don't trust these low BXD8 values. Could be necrosis. Get younger cases. Photos of different thin section by Rob. 2 necrotic fibers Jan-20-96 1 "Box 28, Slot B-4" 3 "12,902 x" 189 x RW RW and RW "3,685 mm^2" "5,263 mm^2" 31.6 µm^2 0.65% 3254 21 325 15.5 ±4.35 ±0.97 #DIV/0! #DIV/0! 12 13 16 7 21 12 13 14 20 17 21 14 11 21 12 11 21 21 18 19 11 BXD8 "grids killed, recut" M 081194E "53,873" "±5,203" 9.7% "108,150" 49.81 -29.685 ? 940104 940811 219 435 0 4 F92 retired breeder from Ben Taylor's colony. "Both grids destroyed after sampling. If duplicate cunt required, will need to recut." none Oct-27-94 19 "Box 28, Slot T-1" 4 "13,062 x" 194 x RCS RW and RW "4,053 mm^2" "5,418 mm^2" 31.8 µm^2 0.65% 3406 22 348 15.8 ±7.00 ±1.53 #DIV/0! #DIV/0! 51627 60662 117.5% " 9,035 " 20 22 12 20 20 34 21 5 11 18 10 11 3 11 18 16 22 13 6 21 17 17 BXD8 original F 081194F "53,844" "±4,722" 8.8% "115,166" 46.75 -29.685 ? 940104 940811 219 448 1 4 F92 retired breeder from Ben Taylor's colony. Old but well fixed 2 necrotic Oct-15-94 21 "Box 29, Slot K-2" 3 "13,033 x" 248 x RCS RCS and RW "7,066 mm^2" "5,418 mm^2" 31.9 µm^2 0.64% 3611 23 343 14.9 ±6.13 ±1.31 #DIV/0! #DIV/0! 56521 58506 103.5% " 1,985 " 19 13 22 18 6 7 15 12 21 20 14 8 2 19 14 27 10 19 19 6 17 18 17 BXD8 replicate 0.00001 "52,704" "±4,803" 9.1% "113,168" 46.57 I don't trust these low BXD8 values. Could be necrosis. Get younger cases. Reshot same thin section. Tried to interleave sample. 6 necrotic fibers Jan-20-96 21 "Box 29, Slot K-2" 2 "13,056 x" 190 x RW RW and RW "4,088 mm^2" "5,263 mm^2" 30.9 µm^2 0.57% 3665 21 302 14.4 ±5.86 ±1.31 #DIV/0! #DIV/0! 18 17 12 5 5 16 15 1 10 13 13 13 15 15 13 21 21 19 20 14 26 BXD8 poor fix M 071795E2 950519 950717 59 23.5 436 F96 perfused by Dan at JAX. Steely grey coat. Very poor fixation. Not even thin sectioned. BXD8 poor fix M 011795L "50,662" "±3,041" 6.0% "66,048" ? 941124 950117 54 20.0 411 6 F93/94. From BenTaylor's colony Unmyelinated fibers cannot be resolved in this poorly fixed tissue. Mar-30-95 14 "Box 41, Slot E-1" 1 "13,053 x" 193 x RW RW and RW "2,465 mm^2" "5,270 mm^2" 30.9 µm^2 1.03% 2135 22 522 23.7 ±6.53 ±1.42 23 19 33 32 28 13 21 29 24 14 25 27 26 14 28 34 21 33 24 22 17 15 BXD8 poor fix M 080393D "36,860" "±4,606" 12.5% "75,691" ? 930630 930803 34 NA 2 F89. Mouse from Ben Taylor's colony. Poor fix at Jackson. Cannot resolve unmyelinated fibers with confidence. Poor fixation. Cannot resolve unmyelinated fibers. Oct-15-94 D4 "Box 24, Slot D-2" 1 "13,105 x" 246 x TK TK and RW "4,577 mm^2" "5,418 mm^2" 31.5 µm^2 0.92% 2399 22 338 15.4 ±8.80 ±1.92 19 20 26 23 33 16 22 17 13 4 13 20 2 21 2 8 9 22 24 1 6 17 BXD8 aged mouse M 072994A "52,346" "±2,838" 5.4% "124,075" ? 920913 940729 684 441 7 Aged (P500) BXD from Ben Taylor's colony. Some necrosis Oct-15-94 D1 "Box 29, Slot P-1" 3 "13,053 x" 193 x RCS RCS and RW "4,630 mm^2" "5,418 mm^2" 31.8 µm^2 0.62% 3902 24 322 13.4 ±3.49 ±0.73 12 16 11 15 16 8 15 14 15 16 12 20 15 10 9 18 12 8 13 14 13 18 16 6 BXD8 aged mouse M 072994C "45,946" "±4,363" 9.5% "120,765" ? 920903 940729 694 428 8 Aged (P500) BXD from Ben Taylor's colony. No pupillary light reflex. Some necrosis Oct-25-94 D5 "Box 29, Slot R-1" 2 "13,083 x" 175 x RW RW and RW "3,679 mm^2" "5,418 mm^2" 31.7 µm^2 0.60% 3815 23 277 12.0 ±5.36 ±1.14 4 5 15 17 10 16 14 8 18 18 5 7 14 11 9 21 18 11 12 18 2 8 16 BXD8 aged mouse M 072994D ? 920903 940729 694 419 8 Aged (P500) BXD from Ben Taylor's colony. No pupillary light reflex in L eye and right eye response also aabnormal. Oct-25-94 BXD9 original F 1 2 080393A "66,053" "±1,611" "±3,602" 6 "61,727" "±2,852" "55,564" "±3,865" 7.0% "111,021" 50.05 -1.201 ? 439 921030 930803 277 NA NA 1 1 F94. Tissue is ok. Mouse from Ben Taylor's colony. none Jan-10-94 D6 "Box 24, Slot G-1" 3 "13,058 x" 246 x TK TK and RW "6,739 mm^2" "5,357 mm^2" 31.4 µm^2 0.82% 3534 29 456 15.7 ±5.79 ±1.09 7.8593E-03 1.2732E-07 52256 66461 127.2% " 14,206 " 8 20 15 4 20 8 19 18 13 17 15 17 5 11 21 15 17 21 11 21 20 30 21 23 17 13 9 15 12 BXD9 replicate 1 0.00001 080393A "64,871" "±4,226" 6.5% "111,844" 58.00 Replicate of different thin section Mar-12-95 D6 "Box 24, Slot G-3" 3 "13,092 x" 193 x RCS DR and RCS "4,179 mm^2" "5,418 mm^2" 31.6 µm^2 0.59% 3538 21 385 18.3 ±5.34 ±1.19 16 19 26 14 16 17 17 25 7 20 20 22 30 14 16 18 18 27 13 15 15 BXD9 replicate 2 0.00001 080393A "64,745" "±5,466" 8.4% "107,869" 60.02 Replicate of another different section Jan-23-96 D6 "Box 24, Slot G-3" 3 "12,988 x" 185 x RW RW and RW "3,705 mm^2" "5,263 mm^2" 31.2 µm^2 0.64% 3457 22 412 18.7 ±7.25 ±1.58 11 14 15 31 12 17 27 22 20 25 18 20 12 35 26 17 15 19 26 6 12 12 BXD9 original F 1 2 080393C "68,260" "±3,330" "62,147" "±5,537" 8.9% "100,881" 61.60 0.613 ? 930529 930803 66 NA NA 1 2 "F96. Tissue has some stain artifact, but count is good. Mouse from Ben Taylor's colony." none Jan-10-94 A6 "Box 24, Slot I-1" 3 "12,897 x" 245 x TK TK and RW "6,039 mm^2" "5,357 mm^2" 32.2 µm^2 0.61% 3132 19 377 19.8 ±7.50 ±1.77 6.4982E-03 9.5198E-08 12 10 25 19 12 39 24 24 25 15 20 28 18 16 26 25 11 17 11 BXD9 replicate F 0.00001 080393C "71,724" "±4,168" 5.8% "105,504" 67.98 Replicate of the same thin section ussed in first analysis. none Mar-12-95 A6 "Box 24, Slot I-1" "13,259 x" 193 x DR RCS and RCS "3,931 mm^2" "5,418 mm^2" 30.8 µm^2 0.61% 3423 21 440 21.0 ±5.44 ±1.22 27 14 17 18 20 30 18 26 20 16 22 19 8 14 23 27 21 28 25 24 23 BXD9 original F 1 2 080393B "69,342" "±3,552" "68,506" "±4,288" 6.3% "139,480" 49.12 0.913 ? 921030 930803 277 NA NA 1 1 F94. Mouse from Ben Taylor's colony. "This nerve was photographed originally on Jan 10, 1994, but the nerve was unstained. This is the reshoot of Jan 18, 1994." Jan-18-94 A3 "Box 24, Slot H-2" 3 "12,960 x" 246 x TK TK and RW "8,466 mm^2" "5,357 mm^2" 31.9 µm^2 0.69% 4373 30 470 15.7 ±5.28 ±0.98 5.6863E-03 8.2004E-08 16 10 23 19 15 20 14 22 12 20 12 21 12 9 6 6 22 13 14 18 24 16 12 23 20 20 6 13 16 16 BXD9 replicate F 0.00001 080393B "71,171" "±6,340" 8.9% "136,931" 51.98 Replicate analysis of different thin section. none Mar-12-95 A3 "Box 24, Slot H-2" "13,082 x" 193 x RW DR and TK "5,095 mm^2" "5,418 mm^2" 31.7 µm^2 0.51% 4325 22 362 16.5 ±6.72 ±1.47 29 5 14 14 16 5 29 17 10 11 25 17 17 15 21 15 16 13 27 18 9 19 BXD9 original M 1 2 081194G "68,775" "±4,099" "75,797" "±5,519" 7.3% "112,034" 67.66 0.756 ? 940106 940811 217 NA 424 0 3 F99. BXD-9; retired breeder. Mouse from Ben Taylor's colony. none Oct-12-94 2 "Box 29, Slot L-1" 4 "13,027 x" 247 x RCS RCS and RW "6,844 mm^2" "5,418 mm^2" 31.9 µm^2 0.57% 3509 20 432 21.6 ±6.85 ±1.57 4.3095E-03 6.2662E-08 26 26 17 22 23 35 19 19 6 27 21 22 15 34 20 26 17 27 17 13 BXD9 replicate M 0.00001 081194G "60,136" "±6,121" 10.2% "114,790" 52.39 Difference was verified and is due strictly to sampling error. Beautiful tissue. Replicate analysis of a different thin section none Mar-12-95 2 "Box 29, Slot L-1" "13,105 x" 193 x RW DR and TK "4,283 mm^2" "5,418 mm^2" 31.5 µm^2 0.52% 3639 19 314 16.5 ±7.14 ±1.68 14 34 15 17 16 23 7 12 10 7 13 31 13 11 19 21 15 15 21 BXD9 original F 1 2 081194H "61,325" "±2,968" "66,614" "±5,309" 8.0% "127,763" 52.14 -1.313 ? 940106 940811 217 NA 456 1 3 F99. BXD-9; retired breeder. Mouse from Ben Taylor's colony. none Oct-15-94 3 "Box 29, Slot M-2" 4 "13,019 x" 251 x RCS RCS and RW "8,020 mm^2" "5,418 mm^2" 32.0 µm^2 0.60% 3997 24 400 16.7 ±6.37 ±1.33 7.2657E-03 1.1848E-07 16 19 24 14 23 22 14 14 11 19 11 26 9 15 25 10 30 18 8 12 22 20 6 12 BXD9 replicate F 0.00001 081194H "58,922" "±3,579" 6.1% "125,916" 46.79 Replicate analysis of a different thin section. Set of photos taken out of focus not included. Rephotographed. Mar-16-95 3 "Box 29, Slot M-2" "12,964 x" 194 x RW RW and RW "4,738 mm^2" "5,418 mm^2" 32.2 µm^2 0.90% 3906 35 528 15.1 ±5.34 ±0.92 18 19 12 20 21 12 21 20 17 8 3 14 9 23 12 14 13 16 9 16 19 21 17 23 20 21 don't count don't count don't count don't count don't count don't count don't count don't count don't count 8 5 15 13 9 20 9 19 12 don't count don't count BXD9 original M 1 2 100494A "66,890" "±3,350" "69,241" "±4,664" 6.7% "78,530" 88.17 0.232 ? 940807 941004 58 22.8 437 0 4 F101. Bought. DBA coloring. "DBA coat color. Formvar grid broken, Area of nerve from adjacent thin." 1 necrotic on 0028 4-Dec-94 2 "Box 33, Slot I-1" 4 "13,050 x" 193 x TK RW and RW "2,925 mm^2" "5,418 mm^2" 31.8 µm^2 0.81% 2468 20 561 28.1 ±8.24 ±1.89 6.2758E-03 9.3823E-08 30 21 30 27 27 47 34 20 22 36 22 22 25 43 28 37 13 21 30 26 BXD9 replicate M 0.00001 100494A "64,387" "±4,813" 7.5% "78,148" 82.39 Replicate of different thin section 11-Mar-95 2 "Box 33, Slot I-3" "13,073 x" 194 x RCS RCS and RCS "2,936 mm^2" "5,418 mm^2" 31.7 µm^2 0.69% 2465 17 444 26.1 ±7.81 ±1.95 40 17 28 15 26 12 15 34 32 25 33 32 27 30 29 20 29 BXD11 original F 1 2 112894G "60,819" "±1,451" "±4,104" 9 "66,474" "±2,941" "69,288" "±3,991" 5.8% "73,226" 94.62 1.378 ? 429 940829 941128 91 22.3 445 1 1 F92 bought from JAX. Photographed on JEOL 1000. Black coat. 1 necrotic on 12 Jan-13-95 13 "Box 35, Slot T-1" 4 "14,778 x" 197 x TK RW and RW "2,844 mm^2" "5,418 mm^2" 24.8 µm^2 0.71% 2951 21 493 23.5 ±6.05 ±1.35 8.0691E-03 1.2139E-07 71676 72401 101.0% 725 33 22 13 21 26 29 29 26 24 31 26 21 19 22 18 23 33 26 25 15 11 BXD11 replicate 0.00001 112894G "63,129" "±4,352" 6.9% "81,729" 77.24 Repeat analysis by Richelle and Rob on different thin section Mar-28-95 13 "Box 35, Slot T-1" "10,947 x" 193 x RCS RW and RW "3,039 mm^2" "5,270 mm^2" 44.0 µm^2 1.72% 1858 32 1087 34.0 ±13.04 ±2.34 duplicate duplicate duplicate duplicate duplicate 45 44 12 15 16 26 34 41 24 28 45 26 39 40 56 37 55 54 42 35 32 15 38 31 51 49 45 33 28 11 19 21 BXD11 F 1 2 112894H "61,608" "±2,461" "61,608" "±2,461" 4.0% "68,119" 90.44 0.192 ? 940829 941128 91 20.5 436 1 1 F92 bought from Jax. Black coat none Jan-17-95 16 "Box 36, Slot B-2" 3 "16,135 x" 193 x RCS RW and RW "2,539 mm^2" "5,418 mm^2" 20.8 µm^2 0.86% 3273 28 527 18.8 ±3.91 ±0.75 1.0548E-02 1.7122E-07 58450 64762 110.8% " 6,313 " 22 16 21 18 17 22 28 25 19 21 17 19 19 16 25 18 23 17 21 10 16 16 17 22 13 16 19 14 BXD11 original F 1 2 112894I "65,290" "±2,632" "69,761" "±4,679" 6.7% "79,963" 87.24 1.090 ? 940908 941128 81 22.0 444 1 2 F92 bought from Jax. Black coat none Jan-17-95 18 "Box 36, Slot C-1" 4 "16,205 x" 193 x RCS RW and RW "2,980 mm^2" "5,418 mm^2" 20.6 µm^2 0.41% 3876 16 288 18.0 ±4.68 ±1.21 1.0057E-02 1.5403E-07 71005 71005 100.0% 28 23 11 where is it 14 16 18 15 18 19 25 13 16 19 16 23 14 BXD11 replicate 0.00001 112894I "63,222" "±3,182" 5.0% "81,218" 77.84 Repeat analysis by Richelle and Rob on different thin section 2 necrotic (on 21 and 45) Mar-28-95 18 "Box 36, Slot C-1" "10,947 x" 193 x RCS RW and RW "3,020 mm^2" "5,270 mm^2" 44.0 µm^2 1.62% 1847 30 1027 34.2 ±9.28 ±1.72 23 20 40 37 33 31 24 23 37 41 24 34 43 51 8 41 46 37 45 37 34 45 22 32 36 39 38 36 30 40 BXD11 original M 1 2 120594C "65,750" "±3,482" "65,239" "±4,128" 6.3% "89,172" 73.16 1.202 ? 941015 941205 51 18.5 420 0 3 F94. Bought from Jax. Black coat 1 necrotic Jan-23-95 8 "Box 36, Slot L-2" 4 "13,157 x" 194 x RCS RW and RW "3,373 mm^2" "5,418 mm^2" 31.3 µm^2 0.70% 2849 20 458 22.9 ±6.32 ±1.45 5.7076E-03 8.6807E-08 60458 69148 114.4% " 8,690 " 21 24 22 24 24 21 30 20 25 blank 34 21 18 21 25 19 36 30 21 11 11 BXD11 replicate 0.00001 120594C "67,012" "±6,486" 9.7% "93,791" 71.45 none Apr-3-95 8 "Box 36, Slot L-3" "10,947 x" 193 x RCS RCS and RW "3,494 mm^2" "5,270 mm^2" 44.0 µm^2 0.89% 2133 19 597 31.4 ±12.90 ±3.04 18 55 29 52 31 16 31 37 41 32 29 49 20 not countable 41 40 29 24 13 10 BXD11 M 1 2 120594D "59,659" "±3,342" "59,659" "±3,342" 5.6% "73,963" 80.66 -0.283 ? 941020 941205 46 17.5 414 0 4 F94. Bought from Jax. Black coat Jan-23-95 11 "Box 36, Slot M-2" 4 "13,113 x" 194 x RCS RW and RW "2,779 mm^2" "5,418 mm^2" 31.5 µm^2 1.24% 2347 29 737 25.4 ±7.53 ±1.42 5.5321E-03 9.2729E-08 61098 59204 96.9% " 1,895 " 27 25 15 26 30 30 31 13 31 29 15 35 41 17 28 21 17 31 18 12 29 33 19 30 20 23 38 26 27 BXD11 original M 1 2 120594E "56,124" "±2,926" "53,140" "±4,961" 9.3% "76,653" 69.33 -1.144 ? 941020 941205 46 17.5 417 0 4 F94. Bought from Jax. "Excellent count. Cannot located low mag calibration, but see 120594C and D. Black coat." 3 necrotic Jan-23-95 13 "Box 36, Slot N-1" 4 "13,098 x" 194 x RCS RW and RW "2,880 mm^2" "5,418 mm^2" 31.6 µm^2 0.78% 2427 19 416 21.9 ±8.67 ±2.04 6.9211E-03 1.2332E-07 50366 57155 113.5% " 6,788 " 25 33 10 23 15 15 34 26 20 18 37 8 28 17 8 27 30 17 25 BXD11 replicate 0.00001 120594E "57,714" "±3,623" 6.3% "76,526" 75.42 Repeat analysis by Richelle and Rob on different thin section Mar-28-95 13 "Box 36, Slot N-1" "10,947 x" 194 x RCS RW and RW "2,881 mm^2" "5,270 mm^2" 44.0 µm^2 1.03% 1740 18 597 33.2 ±8.58 ±2.08 40 40 30 42 24 16 22 32 39 31 42 38 30 50 26 33 37 25 BXD11 F 1 2 071795O-2 "57,119" "±4,788" "57,119" "±4,788" 8.4% "98,980" 57.71 1.236 ? 950509 950717 69 24.6 442 1 5 F96/95. Perfused by Dan at JAX. Black non-agouti coat color. This animal was 11 days pregnant. none Jan-10-96 14 "Box 54, Slot Q-2" 3 "13,006 x" 192 x RW RW and RW "3,637 mm^2" "5,263 mm^2" 31.1 µm^2 0.69% 3181 22 395 18.0 ±6.90 ±1.51 2.6101E-03 4.5696E-08 22 25 16 9 24 16 20 15 17 11 2 20 20 17 31 19 5 22 24 22 13 25 BXD11 F 1 2 071795P-2 "56,988" "±4,505" "56,988" "±4,505" 7.9% "80,570" 70.73 1.167 ? 950509 950717 69 NA 421 1 5 F96/95. Perfused by Dan at JAX. Black non-agouti coat color. none Jan-10-96 16 "Box 54, Slot R-1" 3 "13,079 x" 191 x RW RW and RW "2,931 mm^2" "5,263 mm^2" 30.8 µm^2 0.80% 2619 21 457 21.8 ±7.69 ±1.72 2.9483E-03 5.1736E-08 27 30 36 25 16 25 11 25 29 22 20 28 9 22 17 13 7 24 15 27 29 BXD11 F 1 2 071795Q-2 "58,360" "±4,408" "58,360" "±4,408" 7.6% "92,550" 63.06 1.895 ? 950509 950717 69 25.9 433 1 5 F96/95. Perfused by Dan at JAX. Black non-agouti coat color. This animal was 9 days pregnant. none Jan-10-96 18 "Box 54, Slot S-2" 4 "13,053 x" 191 x RW RW and RW "3,372 mm^2" "5,263 mm^2" 30.9 µm^2 0.77% 2996 23 448 19.5 ±6.90 ±1.47 3.1396E-03 5.3798E-08 23 22 26 27 17 22 11 11 14 10 28 3 25 22 21 17 14 18 20 34 23 22 18 BXD11 poor fixation F 072495L-2 950612 950724 42 17.4 424 1 6 "F95, perfused by Dan at JAX. Black coat." Dan notes that the perfusion may be poor. He was right. Tissue is poor. none Jan-18-96 1 "12,959 x" 190 x RW RW and RW "3,099 mm^2" "5,263 mm^2" 31.3 µm^2 #DIV/0! 0 22 417 19.0 ±5.57 ±1.21 28 23 20 28 13 16 26 24 23 14 15 23 14 17 22 13 9 12 21 13 23 20 BXD12 F 1 2 080393F "54,788" ±943 "±1,886" 5 "55,182" "±4,991" "55,182" "±4,991" 9.0% "87,565" 63.02 0.209 ? 408 930704 930803 30 NA 1 1 F85. Tissue is great. Mouse from Ben Taylor's colony. none Jan-11-94 C3 "Box 24, Slot J-2" 4 "13,068 x" 245 x TK TK and RW "5,249 mm^2" "5,349 mm^2" 31.3 µm^2 0.82% 2796 23 454 19.7 ±8.37 ±1.79 2.3163E-03 4.1977E-08 50722 65442 129.0% " 14,720 " 34 23 6 20 16 22 10 24 39 29 18 26 13 26 22 19 19 17 18 16 3 24 10 BXD12 original F 1 2 080393H "54,180" "±3,346" "48,004" "±4,137" 8.6% "81,200" 59.12 -0.322 ? 930704 930803 30 NA 1 1 F85. Mouse from Ben Taylor's colony. none Jan-12-94 D3 "Box 24, Slot L-1" 4 "13,017 x" 243 x TK TK and RW "4,808 mm^2" "5,411 mm^2" 31.9 µm^2 0.98% 2543 25 472 18.9 ±7.97 ±1.63 5.0396E-03 9.3015E-08 58950 47911 81.3% " 11,038 " 18 12 9 22 24 20 8 15 19 31 10 28 4 35 22 16 24 22 24 31 18 11 22 8 19 BXD12 check: replicate 2 0.00001 080393H "72,303" "±3,192" 4.4% "77,831" 92.90 ? Second replicate of different section. Done to refine estimate. 1 necrotic Feb-1-96 3 "13,066 x" 192 x RW RW and RW "2,884 mm^2" "5,388 mm^2" 31.6 µm^2 0.89% 2466 22 645 29.3 ±5.93 ±1.29 32 26 31 38 28 16 20 32 39 34 28 26 22 36 27 27 33 37 31 22 31 29 BXD12 check: replicate 1 0.00001 080393H "65,880" "±5,693" 8.6% "76,585" 86.02 ? Replicate. Excellent tissue but contrast of negatives is low. Confirm that this is same nerve as original estimate. none Jan-23-96 D3 "Box 24, Slot L-3" 3 "12,984 x" 185 x RW RW and RW "2,623 mm^2" "5,263 mm^2" 31.2 µm^2 0.86% 2453 21 564 26.9 ±10.38 ±2.32 35 7 23 30 1 32 19 34 31 34 21 22 33 33 42 29 39 30 31 11 27 BXD12 F 1 2 080393G "53,045" "±4,138" "53,045" "±4,138" 7.8% "76,999" 68.89 -0.925 ? 930704 930803 30 NA 1 1 F85. Mouse from Ben Taylor's colony. Necrotic fiber on 0029 Jan-19-94 D1 "Box 24, Slot K-2" 4 "12,983 x" 247 x TK TK and RW "4,691 mm^2" "5,364 mm^2" 31.8 µm^2 1.07% 2420 26 570 21.9 ±8.55 ±1.71 3.2221E-03 6.0743E-08 55850 54304 97.2% " 1,546 " 11 8 19 23 26 20 17 27 20 25 31 34 14 15 36 31 37 13 24 22 10 35 11 21 25 15 BXD12 R or L M 1 2 101794B "57,857" "±2,939" "54,960" "±5,005" 9.1% "76,816" 71.55 1.628 R or L 940831 941017 47 18.0 413 0 2 F88. Purchased from Jax. Grey coat. Grey coat Dec-7-94 1 "Box 30, Slot M-2" 4 "13,126 x" 195 x TK RW and RW "2,907 mm^2" "5,418 mm^2" 31.4 µm^2 0.90% 2443 22 495 22.5 ±9.39 ±2.05 7.0176E-03 1.2129E-07 55714 58246 104.5% " 2,532 " 27 29 30 0 28 28 26 23 4 27 33 37 30 16 21 16 14 18 10 21 30 27 BXD12 L or R 0.00001 101794B "59,382" "±3,631" 6.1% "81,252" 73.08 L or R 940831 941017 F88. Purchased from Jax. Grey coat. Other nerve counted by Richelle. none Dec-14-94 21 "Box 30, Slot S-2" "13,045 x" 193 x RCS RW and RW "3,023 mm^2" "5,418 mm^2" 31.8 µm^2 1.02% 2552 26 605 23.3 ±7.11 ±1.42 56792 64618 113.8% " 7,826 " 15 31 24 20 26 20 32 35 31 10 23 15 28 21 23 20 10 17 39 28 25 26 24 24 22 16 BXD12 M 1 2 101794C "53,676" "±4,066" "53,676" "±4,066" 7.6% "76,471" 70.19 -0.590 ? 940831 941017 47 18.3 404 0 2 F88. Purchased from Jax. Grey coat. Nov-29-94 2 "Box 33, Slot H-4" 3 "13,019 x" 192 x RCS RW and RW "2,830 mm^2" "5,418 mm^2" 32.0 µm^2 0.67% 2392 16 359 22.4 ±6.58 ±1.70 3.4635E-03 6.4526E-08 52533 54622 104.0% " 2,089 " 26 28 27 23 30 4 16 32 21 24 18 23 19 20 26 22 BXD13 original M 1 2 011795I "54,101" "±2,086" "±4,171" 5 "56,484" "±2,498" "58,129" "±3,280" 5.6% "81,452" 71.37 0.571 ? 941227 950117 21 19.8 397 0 1 F120. These three cases look older than advertised. Brown coat. This strain has poor fitness. Almost went extinct in 1994. Low mag is correct. This strain and BXD20 maintained (>summer 1994) on NIH 991A diet. All other strains on Wayne Lab Tech High Fish Oil diet. 1 necrotic Feb-15-95 8 "Box 38, Slot G-1" 3 "13,051 x" 182 x TK RCS and RW "2,711 mm^2" "5,418 mm^2" 31.8 µm^2 0.78% 2561 20 454 22.7 ±5.58 ±1.28 9.5264E-03 1.6866E-07 57359 58896 102.7% " 1,536 " 21 32 18 13 22 18 22 17 24 33 23 20 23 28 34 21 19 27 18 21 BXD13 replicate 0.00001 011795I "54,210" "±3,856" 7.1% "79,091" 68.54 Replicate of different section none Jan-26-96 8 "Box 38, Slot G-1" 3 "13,055 x" 246 x RW RW and RW "4,774 mm^2" "5,263 mm^2" 30.9 µm^2 1.17% 2561 30 635 21.2 ±8.11 ±1.51 18 21 23 9 27 31 22 27 27 28 17 21 26 12 12 29 38 20 19 30 8 16 28 23 18 7 15 25 32 6 BXD13 M 1 2 011795J "56,328" "±3,442" "56,328" "±3,442" 6.1% "67,006" 84.06 0.534 ? 941227 950117 21 17.5 393 0 1 F120. These three cases look older than advertised. Brown coat. This strain has poor fitness. This strain and BXD20 are both maintained (>summer 1994) on NIH 991A diet. All other strains on Wayne Lab Tech High Fish Oil diet. Feb-16-95 10 "Box 38, Slot J-2" 4 "12,905 x" 193 x TK RW and RW "2,488 mm^2" "5,418 mm^2" 32.5 µm^2 0.97% 2060 20 547 27.4 ±7.29 ±1.67 5.0035E-03 8.8827E-08 61167 51487 84.2% " 9,680 " 30 21 23 26 20 24 29 16 30 34 39 23 28 20 39 39 22 18 37 29 BXD13 M 1 2 011795K "58,053" "±3,325" "58,053" "±3,325" 5.7% "59,226" 98.02 0.948 ? 941227 950117 21 20.5 416 0 1 F120. These three cases look older than advertised. Brown coat. This strain has poor fitness. This strain and BXD20 are both maintained (>summer 1994) on NIH 991A diet. All other strains on Wayne Lab Tech High Fish Oil diet. Feb-24-95 13 "Box 38, Slot L-2" 3 "13,142 x" 194 x TK TK and TK "2,218 mm^2" "5,418 mm^2" 31.4 µm^2 1.06% 1888 20 615 30.8 ±7.68 ±1.76 5.5275E-03 9.5213E-08 59279 56825 95.9% " 2,454 " 37 47 31 28 18 37 24 32 32 31 36 25 39 19 32 32 19 23 33 40 BXD13 M 1 2 072495J-2 "51,648" "±3,567" "51,648" "±3,567" 6.9% "68,211" 75.72 -0.588 ? 950617 950724 37 18.0 406 0 2 "F123, perfused by Dan at JAX. Brown coat." none Jan-5-96 11 "Box 56, Slot A-1" 4 "12,929 x" 190 x RW RW and RW "2,468 mm^2" "5,263 mm^2" 31.5 µm^2 0.88% 2166 19 453 23.8 ±6.99 ±1.65 4.2846E-03 8.2958E-08 30 30 13 21 18 29 28 23 20 19 20 11 21 36 32 23 17 29 33 BXD13 original M 1 2 072495K-2 "47,993" "±1,841" "49,167" "±2,336" 4.8% "61,423" 80.05 -1.465 ? 950617 950724 37 17.0 399 0 2 "F123, perfused by Dan at JAX. Brown coat." none Jan-5-96 12 "Box 56, Slot B-2" 4 "13,091 x" 191 x RW RW and RW "2,238 mm^2" "5,263 mm^2" 30.7 µm^2 1.20% 2000 24 590 24.6 ±5.60 ±1.17 1.4772E-02 3.0780E-07 24 28 27 26 15 18 36 27 21 31 23 29 14 17 28 21 20 31 31 29 23 28 20 23 BXD13 replicate 0.00001 072495K-2 "46,068" "±2,991" 6.5% "63,170" 72.93 ? 2 Replicate of different section to confirm low estimate. none Jan-26-96 12 "Box 56, Slot B-2" 4 "12,996 x" 192 x RW RW and RW "2,318 mm^2" "5,263 mm^2" 31.2 µm^2 1.09% 2027 22 500 22.7 ±6.76 ±1.48 17 33 20 23 6 29 23 25 25 17 27 26 13 18 17 30 18 24 27 24 36 22 BXD14 F 1 2 051394E "65,283" "±1,628" "±3,987" 7 "61,729" "±5,314" "61,729" "±5,314" 8.6% "75,074" 82.22 -0.891 ? 436 940331 940513 43 17.5 434 1 1 F97. Purchased mouse from Jax none Aug-4-94 1 "Box 27, Slot K2" 4 "12,942 x" 244 x RW RW and RW "4,487 mm^2" "5,443 mm^2" 32.5 µm^2 0.78% 2310 18 481 26.7 ±9.49 ±2.30 2.3142E-03 3.7490E-08 60468 64899 107.3% " 4,431 " 29 39 44 27 35 29 22 20 25 29 32 41 9 16 26 18 27 13 BXD14 F 1 2 051394F "69,081" "±4,212" "69,081" "±4,212" 6.1% "75,853" 91.07 0.953 ? 940331 940513 43 17.0 423 1 1 F97. Purchased mouse from Jax none Aug-4-94 2 "Box 27, Slot L-1" 4 "13,064 x" 245 x RW RW and RW "4,548 mm^2" "5,443 mm^2" 31.9 µm^2 0.88% 2378 21 610 29.0 ±7.92 ±1.77 4.0878E-03 5.9174E-08 71604 66824 93.3% " 4,779 " 25 24 16 31 23 40 35 32 34 21 42 38 25 25 27 25 42 39 18 26 22 BXD14 original F 1 2 051394I "59,564" "±2,569" "60,416" "±3,597" 6.0% "87,408" 69.12 -1.434 ? 940331 940513 43 20.5 418 1 1 F97. Purchased mouse from Jax none Aug-4-94 9 "Box 27, Slot M-2" 4 "12,931 x" 246 x RW RW and RW "5,282 mm^2" "5,443 mm^2" 32.6 µm^2 0.82% 2685 22 495 22.5 ±6.14 ±1.34 9.4518E-03 1.5868E-07 57296 64428 112.4% " 7,131 " 28 12 20 18 26 21 18 26 22 15 25 12 14 30 24 26 38 23 22 24 25 26 BXD14 replicate 0.00001 051394I "58,676" "±3,671" 6.3% "85,709" 68.46 Adjacent section. none Jan-23-96 9 "Box 27, Slot M-2" 4 "12,996 x" 185 x RW RW and RW "2,936 mm^2" "5,263 mm^2" 31.2 µm^2 0.76% 2750 21 448 21.3 ±5.97 ±1.33 22 22 30 18 32 12 17 23 21 19 21 6 28 17 22 27 25 26 24 17 19 BXD14 original M 1 2 101794D "67,261" "±3,404" "71,400" "±5,263" 7.4% "95,463" 74.79 0.496 ? 940818 941017 60 29.8 459 0 2 F98. Purchased mouse from Jax. Non-agouti coat. Black coat Nov-29-94 9 "Box 33, Slot E-1" 3 "13,049 x" 192 x RCS RW and RW "3,527 mm^2" "5,418 mm^2" 31.8 µm^2 0.67% 3000 20 476 23.8 ±7.65 ±1.75 6.1086E-03 9.0819E-08 74998 67798 90.4% " 7,200 " 39 13 17 21 15 24 24 22 20 43 17 19 19 33 27 22 24 31 24 22 BXD14 replicate 0.00001 101794D "64,284" "±4,464" 6.9% "90,808" 70.79 Replicate of different section. Low contrast. none Jan-26-96 9 "Box 33, Slot E-1" 3 "12,987 x" 192 x RW RW and RW "3,346 mm^2" "5,263 mm^2" 31.2 µm^2 0.76% 2910 22 486 22.1 ±7.03 ±1.53 17 21 23 28 12 18 28 18 16 30 14 26 22 18 28 28 26 27 30 10 35 11 BXD14 M 1 2 101794E "62,844" "±4,438" "62,844" "±4,438" 7.1% "83,904" 74.90 -0.612 ? 940905 941017 42 24.8 452 0 3 F98. Purchased mouse from Jax. Non-agouti coat. Black coat Dec-7-94 7 "Box 30, Slot J-2" 3 "13,100 x" 194 x TK RW and RW "3,153 mm^2" "5,418 mm^2" 31.6 µm^2 0.64% 2658 17 402 23.6 ±6.68 ±1.67 3.3903E-03 5.3947E-08 66106 59942 90.7% " 6,164 " 29 28 9 18 23 37 24 26 13 27 24 24 27 23 30 16 24 BXD14 M 1 2 101794F "70,267" "±5,052" "70,267" "±5,052" 7.2% "76,941" 91.33 1.250 ? 940903 941017 44 22.5 431 0 4 F98. Purchased mouse from Jax. Non-agouti coat. Balck coat Dec-14-94 9 "Box 30, Slot T-1" 4 "13,023 x" 193 x RCS RW and RW "2,863 mm^2" "5,418 mm^2" 31.9 µm^2 0.71% 2408 17 496 29.2 ±8.39 ±2.10 2.9252E-03 4.1630E-08 68937 71446 103.6% " 2,509 " 31 11 44 36 28 39 20 31 22 17 30 27 26 34 38 34 28 BXD14 F 1 2 072994H-2 "66,235" "±4,527" "66,235" "±4,527" 6.8% "98,850" 67.01 0.239 ? NA 940729 200 20.5 432 1 4 "Black, non-agouti coat color. Age is greater than 100 days." Oct-27-94 16 "Box 33, Slot D-4" 4 "13,018 x" 192 x RCS RW and RW "3,663 mm^2" "5,418 mm^2" 32.0 µm^2 0.61% 3092 19 407 21.4 ±6.21 ±1.46 3.4108E-03 5.1496E-08 61398 70384 114.6% " 8,987 " 18 19 34 17 21 16 24 16 28 17 34 20 19 23 14 30 13 21 23 BXD15 M 1 2 080994I "64,235" ±989 "±2,211" 6 "62,770" "±5,160" "62,770" "±5,160" 8.2% "93,906" 66.84 -0.663 ? 442 NA 940809 200 457 0 1 "F78 retired breeder From Ben Taylor. Dark brown coat, normal pupillary reflex. Age not listed in Dan's perfusion notes" Oct-30-94 12 "Box 33, Slot M-4" 4 "12,863 x" 192 x RCS RW and RW "3,455 mm^2" "5,418 mm^2" 32.7 µm^2 0.63% 2868 18 394 21.9 ±7.42 ±1.80 2.4960E-03 3.9764E-08 63278 65901 104.1% " 2,623 " 13 32 10 25 16 20 28 15 29 22 23 10 34 21 25 26 15 30 BXD15 original F 1 2 080994J "67,213" "±3,551" "67,011" "±5,751" 8.6% "107,082" 62.58 1.347 ? NA 940809 200 464 1 1 "F78 retired breeder From Ben Taylor. Dark brown coat, normal pupillary reflex. " Oct-30-94 14 "Box 33, Slot L-3" 4 "12,832 x" 192 x RCS RW and RW "3,957 mm^2" "5,418 mm^2" 32.9 µm^2 0.68% 3254 22 453 20.6 ±8.10 ±1.77 5.5837E-03 8.3075E-08 57346 77254 134.7% " 19,908 " 18 15 26 25 26 21 10 17 41 26 10 12 32 9 20 20 20 19 31 18 26 11 BXD15 replicate 0.00001 080994J "67,337" "±4,514" 6.7% "109,708" 61.38 ? Repeat of different section. none Feb-1-96 4 "13,091 x" 191 x RW RW and RW "3,997 mm^2" "5,263 mm^2" 30.7 µm^2 0.56% 3572 20 377 18.9 ±5.51 ±1.26 19 17 22 16 30 10 25 10 22 20 16 15 19 13 15 21 22 13 27 25 BXD15 get date: original M 1 2 100494B "61,004" "±2,799" "58,382" "±3,911" 6.7% "70,436" 82.89 -1.461 ? 940804 941004 61 29.8 457 0 2 Bought. Brown coat. 5 "Box 33, Slot G-3" 4 "12,787 x" 193 x RCS RW and RW "2,621 mm^2" "5,418 mm^2" 33.1 µm^2 0.71% 2126 15 412 27.5 ±6.89 ±1.84 8.3448E-03 1.3679E-07 57027 61227 107.4% " 4,200 " 37 27 21 22 38 28 34 34 36 19 19 26 21 29 21 BXD15 replicate 0.00001 100494B "63,756" "±4,006" 6.3% "71,596" 89.05 Repeat of different section. none Feb-1-96 4 "13,091 x" 191 x RW RW and RW "2,608 mm^2" "5,263 mm^2" 30.7 µm^2 0.99% 2331 23 629 27.3 ±8.06 ±1.72 30 25 22 39 34 15 17 22 31 21 32 36 45 21 35 32 22 37 25 22 26 14 26 BXD15 get info from UT F 1 2 100494C "63,788" "±4,521" "63,788" "±4,521" 7.1% "77,620" 82.18 -0.203 ? 940804 941004 61 22.0 441 1 2 Bought. Brown coat. Oct-31-94 6 "Box 33, Slot O-4" 4 "12,898 x" 193 x RCS RW and RW "2,880 mm^2" "5,418 mm^2" 32.6 µm^2 0.71% 2383 17 455 26.8 ±7.59 ±1.90 3.3164E-03 5.1992E-08 59471 74030 124.5% " 14,558 " 28 16 23 39 37 32 16 27 23 27 11 31 29 35 24 32 25 BXD15 M 1 2 072495A-2 "65,788" "±4,399" "65,788" "±4,399" 6.7% "62,357" 105.50 0.702 950623 950724 31 20.0 415 0 4 "F82/84, perfused by Dan at JAX. Brown coat." Dan noted that the pupillary light reflex was not perfectly normal. Beautiful tissue. none Dec-10-95 13 "B0x 55, Slot Q-2" 4 "13,047 x" 192 x RCS RW and RW "2,307 mm^2" "5,225 mm^2" 30.7 µm^2 1.03% 2032 21 680 32.4 ±9.68 ±2.17 3.5702E-03 5.4269E-08 37 25 43 11 42 22 38 24 26 46 36 46 30 31 29 17 45 36 35 25 36 BXD15 F 1 2 101794G "64,850" "±5,575" "64,850" "±5,575" 8.6% "94,305" 68.77 0.278 ? 940831 941017 47 22.0 419 1 3 F80. Bought. DBA coat noted by Richard in perfusion book. Probably an error of coat color nomenclature. 11 "Box 33, Slot G-3" 4 "12,962 x" 193 x RCS RW and RW "3,509 mm^2" "5,418 mm^2" 32.2 µm^2 0.58% 2924 17 377 22.2 ±7.63 ±1.91 2.2169E-03 3.4184E-08 55852 72684 130.1% " 16,833 " 29 18 36 13 27 12 21 18 20 24 19 18 20 37 32 13 20 BXD16 original M 1 2 052995A "64,255" "±2,517" "±6,166" 7 "55,934" "±4,353" "55,934" "±4,353" 7.8% "70,870" 78.92 -3.755 ? 465 950413 950529 46 22.3 464 463 46 0 1 Grey mice straight from JAX. From F117 none Aug-28-95 19 "Box 48, Slot F-4" 3 "13,079 x" 192 x RCS RW and RW "2,626 mm^2" "5,359 mm^2" 31.3 µm^2 0.80% 2262 18 445 24.7 ±7.93 ±1.92 3.1260E-03 5.5888E-08 23 27 16 40 34 35 24 13 11 20 26 29 22 19 26 24 36 20 BXD16 please reshoot "13,045 x" 193 x RW RW and RW "3,424 mm^2" "5,225 mm^2" 30.7 µm^2 #DIV/0! 0 0 0 #DIV/0! #DIV/0! #DIV/0! BXD16 M 1 2 052995B "65,957" "±4,010" "65,957" "±4,010" 6.1% "78,908" 83.59 0.779 ? 950413 950529 46 25.8 483 484 53 0 1 Grey mice straight from JAX. From F117 none Aug-28-95 20 "Box 48, Slot G-1" 4 "13,082 x" 193 x RCS EGW and RW "2,932 mm^2" "5,359 mm^2" 31.3 µm^2 0.91% 2520 23 602 26.2 ±7.46 ±1.59 4.2893E-03 6.5031E-08 26 24 31 13 21 33 19 34 27 26 33 39 39 18 21 31 26 13 28 29 29 14 28 BXD16 F 1 2 052995C "64,553" "±3,737" "64,553" "±3,737" 5.8% "73,062" 88.35 0.144 ? 950417 950529 42 16.3 432 430 45 1 2 Grey mice straight from JAX. From F117 none Sep-19-95 2 "Box 48, Slot H-2" 3 "12,923 x" 192 x RCS RCS and RW "2,703 mm^2" "5,360 mm^2" 32.1 µm^2 1.23% 2276 28 794 28.4 ±8.53 ±1.64 4.7939E-03 7.4264E-08 34 19 27 40 20 28 32 32 20 27 32 34 25 29 58 19 29 25 31 41 15 29 21 31 23 28 23 22 BXD16 F 1 2 071795F-2 "64,857" "±5,757" "64,857" "±5,757" 8.9% "77,671" 83.50 0.281 ? 950513 950717 65 20.2 440 1 3 F118. Listed by Dan as a brown coat (fixed by Dan at JAX) but should be grey. none Dec-10-95 19 "Box 55, Slot K-2" 4 "13,033 x" 192 x RCS RW and RW "2,870 mm^2" "5,225 mm^2" 30.8 µm^2 0.75% 2525 19 488 25.7 ±9.67 ±2.28 2.0655E-03 3.1848E-08 22 32 32 23 24 21 26 28 27 53 36 27 29 7 24 21 9 20 27 BXD16 F 1 2 071795H-2 "63,742" "±3,324" "63,742" "±3,324" 5.2% "86,038" 74.09 -0.223 ? 950518 950717 60 19.4 467 1 4 F117. List by Dan as a mouse with a steely grey brown coat. none Dec-10-95 1 "Box 55, Slot L-1" 3 "13,015 x" 192 x RCS RW and RW "3,157 mm^2" "5,225 mm^2" 30.8 µm^2 0.72% 2790 20 457 22.9 ±5.19 ±1.19 6.0729E-03 9.5274E-08 28 19 21 21 10 26 23 25 33 20 17 21 22 18 31 21 22 28 25 26 BXD16 F 1 2 071795J-2 "59,131" "±4,487" "59,131" "±4,487" 7.6% "86,810" 68.12 -2.309 ? 950424 950717 84 21.0 474 1 5 F118. List by Dan as a mouse with a steely grey coat. none Jan-10-96 6 "Box 55, Slot M-2" 3 "13,052 x" 191 x RW RW and RW "3,167 mm^2" "5,263 mm^2" 30.9 µm^2 0.78% 2810 22 463 21.0 ±7.32 ±1.60 3.0762E-03 5.2023E-08 16 19 16 20 34 34 28 29 20 30 20 3 23 21 24 14 25 18 23 15 19 12 BXD16 original F 1 2 071795K-2 "75,614" "±7,438" "75,614" "±7,438" 9.8% "92,279" 81.94 5.147 ? 950424 950717 84 23.0 481 1 5 F118. List by Dan as a mouse with a steely grey coat. none Dec-11-95 7 "Box 55, Slot N-1" 4 "13,045 x" 193 x RCS RW and RW "3,424 mm^2" "5,225 mm^2" 30.7 µm^2 0.63% 3006 19 478 25.2 ±10.50 ±2.47 1.4425E-03 1.9077E-08 13 34 22 32 28 12 48 28 23 24 11 37 16 28 26 40 24 25 7 BXD16 please reshoot 1E-08 071795K-2 #DIV/0! -29.055 ? 950424 950717 84 23.0 481 dup "13,045 x" 193 x RW RW and RW "3,424 mm^2" "5,225 mm^2" 30.7 µm^2 #DIV/0! 0 19 478 25.2 ±10.50 ±2.47 13 34 22 32 28 12 48 28 23 24 11 37 16 28 26 40 24 25 7 BXD18 get info from Jax M 1 2 080193C "54,266" "±1,147" "±2,565" 6 "55,306" "±3,219" "55,306" "±3,219" 5.8% "93,991" 58.84 0.406 ? 413 NA 930801 200 NA 0 1 Mouse from Ben Taylor's colony. none 2-Nov-93 B2 "Box 23, Slot K-2" 4 "13,042 x" 247 x TK TK and RW "5,755 mm^2" "5,359 mm^2" 31.5 µm^2 0.80% 2983 24 445 18.5 ±5.18 ±1.08 5.5707E-03 1.0073E-07 55933 57721 103.2% " 1,788 " 9 13 12 19 17 20 28 24 17 13 18 14 24 29 17 17 23 21 19 23 20 22 15 11 BXD18 get info from Jax F 1 2 080193A "54,976" "±4,155" "54,976" "±4,155" 7.6% "59,260" 92.77 0.277 ? NA 930801 200 NA 1 2 Mouse from Ben Taylor's colony. none 2-Nov-93 A2 "Box 23, Slot I-1" 4 "13,070 x" 247 x TK TK and RW "3,620 mm^2" "5,382 mm^2" 31.5 µm^2 1.17% 1881 22 643 29.2 ±10.12 ±2.21 3.3360E-03 6.0681E-08 57221 54179 94.7% " 3,042 " 45 33 54 41 21 38 13 31 37 38 32 28 27 23 31 26 23 18 26 25 18 15 BXD18 F 1 2 051394G "52,659" "±3,338" "52,659" "±3,338" 6.3% "58,117" 90.61 -0.626 ? 940405 940513 38 15.0 400 1 3 F104. Purchased mouse from Jax none 5-Aug-94 4 "Box 27, Slot N-1" 4 "12,925 x" 247 x RW RW and RW "3,550 mm^2" "5,443 mm^2" 32.6 µm^2 1.18% 1784 21 620 29.5 ±8.37 ±1.87 4.9614E-03 9.4217E-08 54691 50690 92.7% " 4,000 " 39 42 13 29 18 27 31 33 24 42 33 25 40 37 29 23 33 34 31 15 22 BXD18 F 1 2 051394H "53,847" "±4,921" "53,847" "±4,921" 9.1% "53,258" 101.11 -0.163 ? 940409 940513 34 16.0 403 1 4 F106. Purchased mouse from Jax none 5-Aug-94 6 "Box 27, Slot O-2" 2 "13,023 x" 248 x RW RW and RW "3,286 mm^2" "5,443 mm^2" 32.1 µm^2 1.21% 1659 20 649 32.5 ±12.93 ±2.97 2.3405E-03 4.3466E-08 50339 56077 111.4% " 5,739 " 59 37 24 30 15 18 46 47 18 15 29 28 39 36 35 36 29 48 13 47 BXD18 "cut other block, get body weight. get info from Jax. " 080193B NA 930801 200 NA Mouse from Ben Taylor's colony. Nerve apparently cut at lamina cribrosa. Too many myelinated fibers and fix not ideal for counting. Cut other block. A4 "Box 23, Slot J-1" BXD18 original M 1 2 100494D "50,627" "±2,710" "59,492" "±5,063" 8.5% "68,312" 87.09 -1.419 ? 941004 60 26.8 428 0 5 Bought. Grey coat. 1 or 2 necrotic 9-Dec-94 9 "Box 33, Slot F-2" 3 "13,015 x" 194 x RCS RW and RW "2,576 mm^2" "5,418 mm^2" 32.0 µm^2 0.66% 2136 14 390 27.9 ±8.55 ±2.37 7.4219E-03 1.4660E-07 56836 66309 116.7% " 9,473 " 19 6 30 39 36 26 29 24 39 33 31 28 26 24 BXD18 replicate 0.00001 100494D "47,065" "±3,209" 6.8% "61,423" 76.62 ? Repeat of different section none 1-Feb-96 3 "13,091 x" 191 x RW RW and RW "2,238 mm^2" "5,388 mm^2" 31.4 µm^2 1.13% 1954 22 530 24.1 ±7.53 ±1.64 32 27 16 18 32 26 19 38 6 22 18 27 15 32 25 35 26 21 28 23 17 27 BXD18 get info from UT M 1 2 100494E "58,177" "±5,212" "58,177" "±5,212" 9.0% "71,150" 81.77 1.525 ? 941004 60 23.5 422 0 5 Bought. Grey coat. none 9-Dec-94 11 "Box 33, Slot T-1" 3 "13,037 x" 192 x RCS RW and RW "2,635 mm^2" "5,418 mm^2" 31.9 µm^2 0.67% 2232 15 391 26.1 ±8.74 ±2.34 2.2945E-03 3.9439E-08 55077 63919 116.1% " 8,842 " 20 39 26 10 26 30 18 34 23 24 13 35 28 25 40 BXD19 original F 1 2 051394J "67,558" "±1,581" "±4,337" 9 "64,058" "±3,195" "57,393" "±4,913" 8.6% "79,727" 71.99 -0.807 ? 423 940321 940513 53 15.3 414 1 1 F100. Purchased mouse from Jax none Aug-4-94 10 "Box 27, Slot P-1" 4 "12,927 x" 247 x RW TK and RW "4,881 mm^2" "5,443 mm^2" 32.6 µm^2 0.82% 2447 20 469 23.5 ±8.75 ±2.01 6.6057E-03 1.0312E-07 63464 53720 84.6% " 9,744 " 28 39 24 33 13 13 17 19 13 40 22 9 20 25 30 28 17 19 31 29 BXD19 replicate 2 0.00001 051394J "66,603" "±4,205" 6.3% "79,418" 83.86 Replicate of different section none Jan-26-96 10 "Box 27, Slot P-1" "13,087 x" 192 x RW RW and RW "2,930 mm^2" "5,263 mm^2" ######## 0.85% 2584 22 567 25.8 ±7.46 ±1.63 40 25 18 31 19 34 22 27 36 20 38 24 31 30 20 21 14 27 33 18 23 16 BXD19 replicate 1 F 0.00001 051394J "66,565" "±4,355" 6.5% "80,392" 82.80 Difference between counts due to sampling error only. Replicate analysis of same section used in first analysis. none Mar-11-95 10 "Box 27, Slot P-1" "12,941 x" 194 x DR RCS and DR "3,033 mm^2" "5,418 mm^2" 32.4 µm^2 0.76% 2485 19 509 26.8 ±7.44 ±1.75 38 32 27 23 23 47 20 23 25 26 20 23 13 28 25 23 34 28 31 BXD19 original M 1 2 081194I "72,792" "±3,315" "70,397" "±4,511" 6.4% "115,687" 60.85 1.207 ? 940127 940811 196 NA 413 0 2 F99 retired breeder from Ben Taylor's colony. Beautiful tissue. none Oct-15-94 6 "Box 29, Slot N-1" 4 "13,043 x" 248 x RCS RCS and RW "7,122 mm^2" "5,418 mm^2" 31.8 µm^2 0.58% 3632 21 407 19.4 ±5.55 ±1.24 6.9535E-03 9.5526E-08 74821 73949 98.8% 872 18 19 9 13 34 20 19 13 25 19 16 19 13 23 22 23 25 24 14 22 17 BXD19 replicate M 0.00001 081194I "75,606" "±4,889" 6.5% "118,979" 63.55 Replicate anlaysis of same section used in first analysis. none Mar-11-95 6 "Box 29, Slot N-1" "13,153 x" 193 x DR TK and RCS "4,440 mm^2" "5,418 mm^2" 31.3 µm^2 0.79% 3799 30 597 19.9 ±6.93 ±1.29 9 24 21 17 25 36 30 14 19 22 14 19 10 15 21 16 20 25 18 27 34 17 21 14 21 17 23 3 24 21 BXD19 original F 1 2 081194J "65,738" "±3,417" "62,869" "±4,373" 7.0% "110,847" 56.72 -0.420 ? 940127 940811 196 NA 431 1 2 F99 retired breeder from Ben Taylor's colony. none Oct-15-94 8 "Box 29, Slot O-2" 4 "13,003 x" 195 x RCS RCS and RW "4,235 mm^2" "5,418 mm^2" 32.0 µm^2 0.66% 3459 23 418 18.2 ±5.93 ±1.26 5.8845E-03 8.9515E-08 66853 63773 95.4% " 3,080 " negatives read 50 higher 21 32 25 20 16 24 27 10 20 19 8 21 19 13 19 9 17 17 14 9 18 21 19 BXD19 replicate 2 0.00001 081194J "62,985" "±5,478" 8.7% "111,434" 56.52 Second replicate of the same section. Jan-26-96 8 "Box 29, Slot O-2" 4 "13,029 x" 192 x RW RW and RW "4,089 mm^2" "5,263 mm^2" ######## 0.58% 3594 21 368 17.5 ±6.82 ±1.52 12 17 20 18 7 25 15 12 5 18 12 20 25 25 19 30 17 22 5 20 24 BXD19 replicate 1 F 0.00001 081194J "73,783" "±5,768" 7.8% "108,613" 67.93 Difference with first sample all due to sampling error Replicate analysis by DR.Get better estimate of nerve area. none Mar-12-95 8 "Box 29, Slot O-2" "13,140 x" 194 x DR RW and RCS "4,104 mm^2" "5,418 mm^2" 31.4 µm^2 0.64% 3461 22 469 21.3 ±7.64 ±1.67 27 13 18 20 15 38 22 33 17 23 30 26 14 15 26 16 11 10 29 27 14 25 BXD19 original F 1 2 072994I-2 "74,741" "±4,602" "76,325" "±5,908" 7.7% "126,594" 60.29 1.656 ? 940729 100 19.8 445 1 3 Bought. Perfused at UT. Grey coat. none Oct-28-94 18 "Box 33, Slot C-3" 2 "12,985 x" 194 x RCS RCS and RW "4,744 mm^2" "5,418 mm^2" 32.1 µm^2 0.61% 3939 24 465 19.4 ±7.19 ±1.50 3.6828E-03 4.9274E-08 68609 84037 122.5% " 15,429 " 23 17 28 22 19 9 23 12 14 19 24 20 5 12 25 7 21 19 24 21 31 35 19 16 BXD19 replicate F 0.00001 072994I-2 "72,297" "±7,339" 10.2% "128,698" 56.18 Replicate of the same thin section. none Mar-12-95 18 "Box 33, Slot C-3" "13,051 x" 193 x RW RCS and RW "4,782 mm^2" "5,418 mm^2" 31.8 µm^2 0.57% 4046 23 411 17.9 ±8.51 ±1.81 16 19 9 27 7 17 18 15 10 23 10 14 18 8 20 21 21 23 44 23 15 5 28 BXD19 original F 1 2 072994J-2 "67,049" "±3,058" "67,090" "±5,879" 8.8% "76,029" 88.24 -0.117 ? 940729 100 19.5 438 1 3 Bought. Perfused at UT. Grey coat. none Oct-28-94 20 "Box 33, Slot B-4" 4 "13,009 x" 192 x RCS RCS and RW "2,817 mm^2" "5,418 mm^2" 32.0 µm^2 0.67% 2375 16 452 28.3 ±9.59 ±2.48 7.6469E-03 1.1405E-07 59435 78333 131.8% " 18,897 " 39 26 32 19 30 24 12 15 35 37 26 24 51 25 32 25 BXD19 replicate F 0.00001 072994J-2 "67,033" "±3,581" 5.3% "75,007" 89.37 Replicate analysis of same section used in original analysis. none Mar-11-95 20 "Box 33, Slot B-4" "13,237 x" 194 x DR TK and DR "2,811 mm^2" "5,418 mm^2" 30.9 µm^2 0.91% 2426 22 608 27.6 ±6.77 ±1.48 26 29 18 25 34 26 37 37 25 29 25 22 33 20 30 30 17 16 35 39 33 22 BXD19 original F 1 2 072994K-2 "64,795" "±3,877" "61,684" "±6,118" 9.9% "96,921" 63.64 -0.637 ? 940729 100 20.8 452 1 3 Bought. Perfused at UT. Grey coat. none Oct-28-94 1 "Box 33, Slot A-3" 3 "13,065 x" 193 x RCS RCS and RW "3,627 mm^2" "5,418 mm^2" 31.7 µm^2 0.65% 3054 20 404 20.2 ±8.73 ±2.00 4.5370E-03 7.0021E-08 66035 61004 92.4% " 5,031 " 29 20 11 29 22 5 14 27 15 12 25 14 6 32 35 22 30 12 23 21 BXD19 replicate F 0.00001 072994K-2 "66,882" "±5,012" 7.5% "102,744" 65.10 Replicate analysis of different thin section. "4 necrotic fibers total, on 0030, 0036, and 0043" Mar-11-95 1 "Box 33, Slot A-1" "13,102 x" 194 x DR TK and DR "3,850 mm^2" "5,418 mm^2" 31.6 µm^2 0.68% 3255 22 452 20.5 ±7.06 ±1.54 20 12 22 26 19 27 21 38 24 22 17 20 16 10 12 17 14 31 28 11 18 27 BXD19 original F 1 2 101794H "66,252" "±2,704" "62,301" "±3,969" 6.4% "76,237" 81.72 -0.301 ? 940910 941017 37 16.0 403 1 4 F101. Bought. Perfused at UT. Grey coat. Dec-14-94 12 "Box 34, Slot H-4" 4 "13,130 x" 192 x THK RW and RW "2,820 mm^2" "5,418 mm^2" 31.4 µm^2 0.91% 2426 22 565 25.7 ±7.50 ±1.64 9.4935E-03 1.4329E-07 63209 66186 104.7% " 2,977 " 16 21 23 27 37 5 18 23 18 21 23 26 31 34 35 27 32 32 32 30 24 30 BXD19 replicate F 0.00001 101794H "69,673" "±3,693" 5.3% "75,040" 92.85 "Replicate analysis of different thin section. RCS to photograph, RW to count, RW to calibrate" 1 necrotic on 0037 Mar-13-95 12 "Box 34, Slot H" 4 "13,138 x" 193 x RCS RW and RW "2,792 mm^2" "5,418 mm^2" 31.4 µm^2 0.88% 2391 21 612 29.1 ±6.91 ±1.54 31 14 18 33 22 28 34 17 24 31 24 35 28 37 31 34 39 37 32 31 32 BXD19 original M 1 2 101794I "70,941" "±2,572" "69,533" "±3,001" 4.3% "79,726" 87.22 0.780 ? 940716 941017 93 22.0 397 0 5 F100. Bought. Perfused at UT. Grey coat. 0.3602 gm Dec-14-94 14 "Box 34, Slot I-1" 4 "13,130 x" 192 x THK RW and RW "2,949 mm^2" "5,418 mm^2" 31.4 µm^2 0.87% 2537 22 603 27.4 ±5.42 ±1.18 1.1236E-02 1.5838E-07 73926 68493 92.7% " 5,432 " 30 20 23 24 26 28 28 28 27 19 33 36 42 31 30 31 30 26 23 23 21 24 BXD19 replicate M 0.00001 101794I "74,831" "±4,989" 6.7% "79,004" 0.00 replicate Mar-13-95 14 "Box 34, Slot I-3" 3 "12,988 x" 192 x RCS RCS and RCS "2,927 mm^2" "5,418 mm^2" 32.1 µm^2 0.77% 2460 19 578 30.4 ±8.60 ±2.03 46 34 34 34 35 18 36 40 17 21 21 25 17 32 38 30 41 32 27 BXD19 original M 1 2 101794J "61,653" "±2,820" "58,663" "±3,790" 6.5% "87,943" 66.71 -1.362 ? 940716 941017 93 23.0 417 0 5 F100. Bought. Perfused at UT. Grey coat. Sample photographs are poorly focused. Dec-15-94 17 "Box 34, Slot J-2" 3 "13,029 x" 192 x RW RW and RW "3,259 mm^2" "5,418 mm^2" 31.9 µm^2 0.87% 2755 24 511 21.3 ±6.60 ±1.38 8.0912E-03 1.3124E-07 58724 62275 106.0% " 3,552 " 27 14 21 9 9 19 28 27 20 22 32 27 22 21 12 19 25 15 14 32 29 22 24 21 BXD19 replicate 2 0.00001 101794J "59,121" "±4,220" 7.1% "89,003" 66.43 Replicate of different section. none Jan-26-96 17 "Box 32, Slot J-4" 4 "12,947 x" 193 x RW RW and RW "3,307 mm^2" "5,263 mm^2" 31.4 µm^2 0.74% 2835 21 438 20.9 ±6.66 ±1.49 24 19 29 16 31 17 31 20 19 14 30 21 11 12 20 22 17 18 33 22 12 BXD19 replicate 1 M 0.00001 101794J "66,589" "±3,754" 5.6% "92,612" 71.90 "Replicate analysis of different thin section. DR to photograph, RCS to count" none Mar-11-95 17 "Box 34, Slot J-2" "13,044 x" 192 x RW RW and RW "3,427 mm^2" "5,418 mm^2" 31.8 µm^2 0.65% 2908 19 435 22.9 ±5.48 ±1.29 24 18 20 18 25 28 24 13 23 28 18 25 33 24 17 24 17 22 34 BXD20 get info from Jax M 1 2 080994H "62,184" "±1,382" "±3,909" 9 "65,018" "±4,562" "65,018" "±4,562" 7.0% "79,521" 81.76 0.725 ? 381 940214 940809 176 22.0 397 433 47 0 1 "Perfused at Jax. Retired breeder. Mouse from Ben Taylor's colony. Richelle reweighed brain at 0.424 gm on Dec 7, 1994" This strain and BXD13 are both maintained (>summer 1994) on NIH 991A diet. All other strains on Wayne Lab Tech High Fish Oil diet. Poor fitness. Moved to back of colony room (away from noisy air system) in 1995. none Oct-31-94 9 "Box 33, Slot N-3" 4 "13,053 x" 193 x RCS RCS and RW "2,976 mm^2" "5,418 mm^2" 31.8 µm^2 0.68% 2501 17 442 26.0 ±7.30 ±1.82 3.3193E-03 5.1052E-08 69733 69103 99.1% 630 35 26 31 24 26 35 30 25 22 26 29 26 25 38 22 9 13 BXD20 original M 1 2 112194P "55,879" "±3,680" "52,038" "±5,565" 10.7% "100,002" 52.04 -1.613 ? 940801 941121 112 21.3 380 363 41 0 2 F48N5F42. Bought from JAX. Age stated to be greater than P100. This strain and BXD13 are both maintained (>summer 1994) on NIH 991A diet. Please check these data again and make note. Black coat. none Dec-23-94 16 "Box 35, Slot F-2" 4 "16,228 x" 193 x THK RW and RW "3,737 mm^2" "5,418 mm^2" 20.6 µm^2 0.35% 4861 17 182 10.7 ±4.58 ±1.14 4.3834E-03 7.8445E-08 52004 52751 101.4% 748 7 9 12 20 3 14 10 7 8 15 18 13 10 12 5 13 6 BXD20 replicate 0.00001 112194P "58,865" "±4,907" 8.3% "102,681" 57.33 ? 940801 941121 dup F48N5F42. Bought from JAX. Age stated to be greater than P100. none Jan-23-95 16 "Box 35, Slot F-1" 4 "13,122 x" 194 x THK TK and TK "3,864 mm^2" "5,418 mm^2" 31.5 µm^2 0.80% 3263 26 469 18.0 ±7.52 ±1.50 56729 60996 107.5% " 4,267 " 13 23 30 28 16 17 17 25 4 22 25 20 16 14 27 14 15 32 23 19 10 3 6 17 19 14 BXD20 original F 1 2 112194Q "61,253" "±3,003" "58,836" "±5,057" 8.6% "59,283" 99.25 -0.238 ? 940911 941121 71 17.0 366 347 38 1 3 F48N5F43. Bought from JAX. This strain and BXD13 are both maintained (>summer 1994) on NIH 991A diet. Black coat. none Dec-23-94 13 "Box 35, Slot G-1" 4 "16,122 x" 192 x THK RW and RW "2,191 mm^2" "5,418 mm^2" 20.8 µm^2 0.56% 2844 16 331 20.7 ±6.89 ±1.78 7.2459E-03 1.1830E-07 58738 57889 98.6% 849 28 28 22 17 16 30 20 24 17 19 15 23 29 16 3 24 BXD20 replicate 0.00001 112194Q "62,568" "±3,732" 6.0% "58,745" 106.51 ? Repeat of different section none Feb-1-96 "13,089 x" 193 x RW RW and RW "2,191 mm^2" "5,396 mm^2" 31.5 µm^2 1.18% 1865 22 738 33.5 ±9.17 ±2.00 54 37 33 35 23 39 37 22 47 37 34 43 34 21 17 28 19 36 33 36 31 42 BXD20 F 1 2 112194R "62,208" "±4,706" "62,208" "±4,706" 7.6% "58,917" 105.59 0.006 ? 940911 941121 71 20.5 393 372 40 1 3 F48N5F43. Bought from JAX. Black coat. none Dec-23-94 15 "Box 35, Slot H-2" 4 "15,916 x" 193 x THK RW and RW "2,198 mm^2" "5,418 mm^2" 21.4 µm^2 0.54% 2755 15 339 22.6 ±6.39 ±1.71 3.0101E-03 4.8388E-08 61475 66522 108.2% " 5,047 " 13.75 21 21 19 16 13 36 28 25 25 18 23 21 30 29 BXD20 original F 1 2 112194S "66,839" "±7,193" "66,839" "±7,193" 10.8% "93,841" 71.23 1.191 ? 940911 941121 71 17.3 368 353 38 1 3 F48N5F43. Bought from JAX. 0.3376 gm brain. Black coat. none Jan-17-94 17 "Box 36, Slot E-1" 4 "16,263 x" 193 x RCS RW and RW "3,498 mm^2" "5,418 mm^2" 20.5 µm^2 0.48% 4581 22 321 14.6 ±7.20 ±1.57 1.3534E-03 2.0248E-08 69389 68958 99.4% 431 0 23 17 18 9 14 26 18 11 5 6 7 29 12 8 19 23 16 18 17 13 12 BXD20 replicate 0.00001 112194S ? Repeat of different section Feb-1-96 "13,089 x" 193 x RW "3,498 mm^2" "5,263 mm^2" 30.7 µm^2 #DIV/0! 0 0 0 #DIV/0! #DIV/0! #DIV/0! BXD20 M 1 2 013095R "60,865" "±6,122" "60,865" "±6,122" 10.1% "110,730" 54.97 -0.337 ? 941206 950130 55 16.8 380 379 41 0 4 F48N5F42. Bought from Jax. a/a coat Black coat. This strain and BXD13 are both maintained (>summer 1994) on NIH 991A diet) none Mar-30-95 4 "Box 40, Slot Q-2" 4 "13,089 x" 193 x RCS RW and RW "4,129 mm^2" "5,270 mm^2" 30.8 µm^2 0.61% 3600 22 372 16.9 ±7.79 ±1.70 1.7011E-03 2.7948E-08 18 4 13 13 14 10 17 31 30 15 16 29 25 22 23 21 8 19 16 10 16 2 BXD20 M 1 2 012795P "68,289" "±3,697" "68,289" "±3,697" 5.4% "107,720" 63.39 1.562 ? 941205 950127 53 16.8 389 388 43 0 5 F48N5F43. Bought from Jax. This strain and BXD13 are both maintained (>summer 1994) on NIH 991A diet) none Apr-3-95 9 "Box 40, Slot S-8" 3 "13,125 x" 194 x RCS RCS and RW "4,070 mm^2" "5,270 mm^2" 30.6 µm^2 1.08% 3521 38 737 19.4 ±6.39 ±1.05 5.1321E-03 7.5153E-08 11 17 13 26 13 19 22 19 27 10 12 20 23 13 19 21 25 14 23 30 9 5 21 23 23 23 22 18 24 22 17 27 33 7 22 24 16 24 BXD20 please reshoot 1E-08 012795P BXD20 M 072495M-2 950620 950724 34 16.7 399 6 "F48N5, F45" Perfused by Dan at JAX. This mouse may have a slightly unusual pupilary light reflex. BXD20 M 072495N-2 950620 950724 34 15.0 381 6 "F48N5, F45" Perfused by Dan at JAX. BXD20 M 072495O-2 950620 950724 34 17.0 374 6 "F48N5, F45" Perfused by Dan at JAX. BXD20 M 1 2 012795D "59,472" "±6,066" "59,472" "±6,066" 10.2% "100,051" 59.44 -0.694 ? 941206 950127 52 15.5 373 368 39 0 4 F48N5F42. Bought from Jax. Lots of glial processes that look similar to unmyelinated axons. Excluded from count by Rob. none Apr-3-95 41 "Box 41, Slot H-1" 4 "10,945 x" 194 x RCS RCS and RW "3,748 mm^2" "5,270 mm^2" 44.0 µm^2 0.88% 2274 20 523 26.2 ±11.63 ±2.67 1.7011E-03 2.8603E-08 41 44 19 28 37 37 27 27 15 16 9 18 22 17 13 9 30 30 47 37 BXD20 M 1 2 030695C "59,832" "±4,271" "59,832" "±4,271" 7.1% "72,040" 83.05 -0.602 ? 941206 950306 90 17.0 357 0 4 F48N5F42. Bought from Jax. maybe 1 Jun-14-95 15 "Box 47, Slot D-2" 4 "12,918 x" 192 x RCS RCS and RW "2,654 mm^2" "5,270 mm^2" 31.6 µm^2 0.96% 2281 22 577 26.2 ±8.58 ±1.87 3.4359E-03 5.7426E-08 3 30 29 25 26 18 35 25 27 29 27 25 18 38 16 23 31 39 23 34 39 17 BXD21 original M 1 2 081194A "59,968" "±1,422" "±3,763" 8 "61,159" "±3,143" "56,634" "±4,770" 8.4% "85,696" 66.09 0.317 ? 444 940418 940811 115 23.0 445 449 44 0 1 F104 retired breeder. Dilute brown mouse. Did not check pupillary response. Mouse from Ben Taylor's colony. "Brain reweighed by Richell Dec 7, 1994 at 0.445 gm. Original value of 0.417 by Richard?" Sep-26-94 11 "Box 28, Slot J-2" 4 "13,003 x" 193 x RCS RCS and RW "3,198 mm^2" "5,418 mm^2" 32.0 µm^2 0.64% 2674 17 360 21.2 ±7.13 ±1.78 6.5763E-03 1.0753E-07 57281 62934 109.9% " 5,653 " 26 35 18 24 18 17 14 23 19 15 19 21 37 8 25 18 23 BXD21 replicate 0.00001 081194A "64,632" "±4,179" 6.5% "88,405" 73.11 Lovely large axons none Jan-20-96 11 "Box 28, Slot J-4" 4 "13,012 x" 191 x RW RW and RW "3,221 mm^2" "5,263 mm^2" 31.1 µm^2 0.77% 2844 22 500 22.7 ±6.73 ±1.47 #DIV/0! #DIV/0! 27 21 28 20 18 28 29 14 17 21 21 21 19 35 18 26 36 8 28 25 25 15 BXD21 original F 1 2 081194B "55,673" "±3,775" "55,492" "±4,922" 8.9% "94,645" 58.63 -1.141 ? 940418 940811 115 20.8 460 460 47 1 1 F104 retired breeder. Normal light response. Mouse from Ben Taylor's colony. "Brain reweighed by Richell Dec 7, 1994 at 0.460 gm. Original value of 0.452 by Richard?" Sep-27-94 12 "Box 28, Slot Q-2" 4 "12,834 x" 192 x RCS RCS and RW "3,487 mm^2" "5,418 mm^2" 32.9 µm^2 0.73% 2877 21 405 19.3 ±7.65 ±1.71 4.1022E-03 7.3683E-08 63445 56829 89.6% " 6,616 " 4 22 29 23 25 15 7 26 18 14 19 25 26 22 22 22 4 24 30 11 17 BXD21 replicate 0.00001 081194B "55,931" "±5,883" 10.5% "97,836" 57.17 Repeat analysis of different section none Jan-20-96 12 "Box 28, Slot Q-2" 4 "12,945 x" 190 x RW RW and RW "3,539 mm^2" "5,263 mm^2" 31.4 µm^2 0.71% 3115 22 395 18.0 ±8.65 ±1.89 #DIV/0! #DIV/0! 18 20 14 5 12 13 13 24 9 15 20 9 17 9 28 40 17 19 33 25 26 9 BXD21 M 1 2 080994E "57,185" "±3,867" "57,185" "±3,867" 6.8% "108,902" 52.51 -0.740 ? 940122 940809 199 NA 435 439 42 0 2 F103 retired breeder from Ben Taylor's colony. Brown (sand DB) Sep-26-94 3 "Box 28, Slot G-1" 4 "13,028 x" 194 x RCS RCS and RW "4,092 mm^2" "5,418 mm^2" 31.9 µm^2 0.62% 3412 21 352 16.8 ±5.07 ±1.13 4.0162E-03 7.0231E-08 60222 59283 98.4% 939 10 20 13 19 20 21 18 12 24 18 21 10 13 24 20 10 6 17 22 18 16 BXD21 F 1 2 080994F "63,488" "±3,817" "63,488" "±3,817" 6.0% "89,814" 70.69 0.935 ? 940122 940809 199 NA 448 454 46 1 2 F103 retired breeder from Ben Taylor's colony. Brown (sand DB) Sep-26-94 5 "Box 28, Slot H-4" 4 "13,070 x" 193 x RCS RCS and RW "3,358 mm^2" "5,418 mm^2" 31.7 µm^2 0.67% 2832 19 426 22.4 ±5.72 ±1.35 4.6005E-03 7.2463E-08 63533 71258 112.2% " 7,725 " 12 25 17 27 27 34 25 23 27 24 16 19 22 19 19 30 26 13 21 BXD21 original M 1 2 100494F "60,273" "±2,797" "55,968" "±4,358" 7.8% "80,431" 69.59 0.081 ? 941004 58 26.0 452 438 44 0 3 Bought. Perfused at UT. DBA coat. First series by Rob labeled 101794K on negatives. This original series is poorly focused. Redone by Toya. none Dec-15-94 13 "Box 34, Slot K-2" 3 "13,029 x" 193 x RW RW and RW "2,989 mm^2" "5,418 mm^2" 31.9 µm^2 0.75% 2520 19 422 22.2 ±7.34 ±1.73 8.1335E-03 1.3495E-07 59356 52916 89.2% " 6,440 " 33 18 22 25 12 23 8 30 16 21 26 24 23 38 26 12 26 21 18 BXD21 replicate 0.00001 100494F "63,286" "±3,647" 5.8% "79,499" 79.61 ? Bought. Perfused at UT. DBA coat. "First series by Rob (Dec 15, 1994) labeled 101794K on negatives. This original series is poorly focused. Redone by Toya." none Jan-23-95 13 "Box 34, Slot K-4" 4 "13,083 x" 195 x TK TK and RW "3,029 mm^2" "5,418 mm^2" 31.7 µm^2 0.80% 2511 20 504 25.2 ±6.33 ±1.45 62280 64289 103.2% " 2,009 " 24 20 28 28 16 19 27 31 33 20 22 28 34 28 38 27 20 19 14 28 BXD21 original F 1 2 100494G "64,885" "±2,676" "63,476" "±4,413" 7.0% "75,167" 84.45 1.307 ? 941004 58 19.3 424 412 39 1 3 Bought. DBA coat. Dec-12-94 15 "Box 30, Slot P-1" 4 "13,018 x" 193 x RCS RW and RW "2,800 mm^2" "5,418 mm^2" 32.0 µm^2 0.72% 2351 17 459 27.0 ±7.51 ±1.88 9.6279E-03 1.4838E-07 64289 65999 102.7% " 1,710 " 41 33 23 37 18 17 22 14 27 36 35 25 27 23 24 28 29 BXD21 replicate 0.00001 100494G "65,705" "±3,365" 5.1% "80,614" 81.51 ? Repeat of different section Jan-26-96 15 "Box 30, Slot P-3" 4 "12,845 x" 193 x RW RW and RW "2,995 mm^2" "5,263 mm^2" 31.9 µm^2 0.87% 2527 22 572 26.0 ±6.10 ±1.33 #DIV/0! #DIV/0! 34 35 19 26 15 31 30 30 24 30 32 19 21 28 27 27 30 32 19 28 21 14 BXD21 F 1 2 040695J "62,464" "±2,988" "62,464" "±2,988" 4.8% "78,981" 79.09 0.663 ? 950121 950406 75 26.0 489 473 53 1 4 F107 from JAX Perfused by Rob and Richelle. Perfusion rated good. Gray brown. none May-23-95 7 "Box 43, Slot Q-2" 3 "13,055 x" 194 x RCS RW and RW "2,972 mm^2" "5,270 mm^2" 30.9 µm^2 0.86% 2554 22 538 24.5 ±5.36 ±1.17 7.3297E-03 1.1734E-07 17 22 28 27 16 18 22 31 19 34 17 22 23 26 30 20 25 23 29 33 27 29 BXD21 M 1 2 040695K "54,618" "±2,584" "54,878" "±3,430" 6.2% "81,769" 67.11 -1.422 ? 950104 950406 92 33.5 440 425 43 0 5 F107 from JAX Perfused by Rob and Richelle. Perfusion rated good. Gray brown coat. 2 or 3 necrotic fibers May-30-95 10 "Box 43, Slot R-1" 3 "12,958 x" 246 x RW RCS and RW "4,968 mm^2" "5,270 mm^2" 31.4 µm^2 1.19% 2605 31 653 21.1 ±7.21 ±1.32 8.4499E-03 1.5471E-07 21 31 14 30 12 18 25 17 30 14 35 26 12 18 19 12 16 15 14 16 26 27 11 34 29 18 20 30 27 16 20 BXD21 replicate 0.00001 040695K "54,277" "±3,931" 7.2% "83,909" 64.69 "Ugly tissue, but the count is ok." 1 necrotic fiber Jan-26-96 2 "12,991 x" 192 x RW RW and RW "3,096 mm^2" "5,263 mm^2" 31.2 µm^2 0.85% 2690 23 464 20.2 ±6.85 ±1.46 #DIV/0! #DIV/0! 26 34 11 8 14 20 23 20 17 20 23 18 15 29 23 15 26 25 26 14 10 16 31 BXD22 M 1 2 052995D "64,802" "±1,869" "±3,738" 5 "70,785" "±3,585" "70,785" "±3,585" 5.1% "80,899" 87.50 1.600 ? 422 950421 950529 38 16.8 423 0 1 Straight from JAX. Grey mouse from F93 none Aug-28-95 3 "Box 48, Slot I-1" 4 "13,170 x" 192 x RCS EGW and RW "2,998 mm^2" "5,418 mm^2" 31.2 µm^2 0.81% 2590 21 574 27.3 ±6.19 ±1.38 5.7822E-03 8.1687E-08 35 31 32 24 27 22 19 32 26 39 15 32 29 26 23 33 18 27 29 21 34 BXD22 M 1 2 052995E "60,994" "±3,562" "60,994" "±3,562" 5.8% "62,368" 97.80 -1.019 ? 950421 950529 38 16.5 417 0 1 Straight from JAX. Grey mouse from F93 none Aug-28-95 6 "Box 48, Slot J-2" 4 "13,065 x" 192 x RCS RW and RW "2,311 mm^2" "5,418 mm^2" 31.7 µm^2 1.27% 1965 25 776 31.0 ±8.88 ±1.81 5.0069E-03 8.2088E-08 30 25 22 31 27 31 41 11 35 34 42 44 39 36 37 27 35 26 35 40 39 24 33 9 23 BXD22 F 1 2 052995F "64,047" "±4,636" "64,047" "±4,636" 7.2% "76,482" 83.74 -0.202 ? 950418 950529 41 14.3 427 1 2 "F93/92. Born between April 13 and April 21, 1995. Grey mouse" 1 or 2 necrotic Sep-19-95 8 "Box 48, Slot K-2" 2 "12,919 x" 192 x RCS RCS and RW "2,822 mm^2" "5,360 mm^2" 32.1 µm^2 0.80% 2381 19 511 26.9 ±8.26 ±1.95 3.1458E-03 4.9117E-08 34 26 23 18 29 22 15 24 38 11 27 14 24 32 34 35 38 33 34 BXD22 M 1 2 072495B-2 "65,513" "±5,061" "65,513" "±5,061" 7.7% "55,795" 117.42 0.190 ? 950617 950724 37 18.2 439 0 3 "F94/93, perfused by Dan at JAX. Steely grey." 1 necrotic Oct-27-95 15 "Box 55, Slot R-1" 4 "12,814 x" 192 x RCS RW and RW "2,047 mm^2" "5,329 mm^2" 32.5 µm^2 1.11% 1719 19 724 38.1 ±12.49 ±2.94 2.6996E-03 4.1208E-08 27 33 22 34 40 47 42 39 17 65 54 33 45 42 46 43 23 51 21 BXD22 M 1 2 072495C-2 "62,671" "±4,568" "62,671" "±4,568" 7.3% "67,722" ? 950617 950724 37 20.8 443 0 3 "F94/93, perfused by Dan at JAX. Steely grey." Oct-27-95 17 "Box 55, Slot S-2" 4 "12,821 x" 192 x RCS RW and RW "2,492 mm^2" "5,329 mm^2" 32.4 µm^2 0.96% 2089 20 600 30.0 ±9.53 ±2.19 3.1617E-03 5.0448E-08 30 31 31 39 23 18 30 31 32 41 43 29 28 36 18 12 38 35 11 44 BXD23 F 1 2 112894J "54,535" "±1,981" "±4,429" 6 "53,435" "±4,888" "53,435" "±4,888" 9.1% "79,846" 66.92 -0.248 ? 421 940904 941128 85 18.5 442 1 1 F20N5F82 bought from Jax. Non-agouti Black coat none Jan-17-95 19 "Box 36, Slot D-2" 3 "16,247 x" 193 x RCS RW and RW "2,977 mm^2" "5,418 mm^2" 20.5 µm^2 0.49% 3890 19 261 13.7 ±5.33 ±1.26 2.3603E-03 4.4172E-08 54846 51864 94.6% " 2,982 " 10 15 7 10 20 11 17 16 6 9 9 6 14 22 21 18 15 13 22 BXD23 F 1 2 112894K "60,873" "±4,828" "60,873" "±4,828" 7.9% "76,912" 79.15 1.431 ? 940904 941128 85 18.8 433 1 1 F20N5F82 bought from Jax. Black coat none Jan-19-95 1 "Box 36, Slot J" 3 "13,084 x" 194 x THK RW and RW "2,892 mm^2" "5,418 mm^2" 31.7 µm^2 0.82% 2430 20 501 25.1 ±8.66 ±1.99 2.7490E-03 4.5159E-08 58320 63423 108.8% " 5,103 " 11 28 28 22 25 27 23 34 21 25 23 20 35 23 23 30 47 24 4 28 BXD23 F 1 2 112894L "50,750" "±4,470" "50,750" "±4,470" 8.8% "76,142" 66.65 -0.855 ? 940912 941128 77 17.3 435 1 2 F20N5F84 bought from JAX. Black coat none Jan-23-95 2 "Box 36, Slot K-2" 4 "13,095 x" 194 x RCS RW and RW "2,880 mm^2" "5,418 mm^2" 31.6 µm^2 0.71% 2410 17 358 21.1 ±7.42 ±1.86 2.6981E-03 5.3165E-08 45486 55426 121.9% " 9,941 " 16 13 28 25 19 12 22 24 23 18 19 14 17 17 43 28 20 BXD23 M 1 2 040695A "51,976" "±3,877" "51,976" "±3,877" 7.5% "74,319" 69.94 -0.578 950106 950406 90 22.7 414 0 3 F20N5f 86 bought from Jax. Fixed by Rob (listed as fair fixation but performance was actually good to excellent). Black color May-14-95 10 "Box 43, Slot J-2" 3 "12,943 x" 193 x RCS RW and RW "2,769 mm^2" "5,270 mm^2" 31.5 µm^2 0.85% 2363 20 440 22.0 ±7.15 ±1.64 3.6405E-03 7.0042E-08 30 24 30 26 8 11 21 28 17 26 11 28 31 24 14 22 23 18 31 17 BXD23 M 1 2 040695B "50,893" "±3,290" "50,893" "±3,290" 6.5% "66,045" 77.06 -0.822 950106 950406 90 20.0 401 0 3 F20N5f 86 bought from Jax. Fixed by Rob (listed as good fixation: performance was actually good to excellent). Black color May-14-95 12 "Box 43, Slot K-1" 4 "12,943 x" 194 x RCS RW and RW "2,475 mm^2" "5,270 mm^2" 31.5 µm^2 1.19% 2100 25 606 24.2 ±7.68 ±1.57 4.8971E-03 9.6224E-08 20 18 20 29 21 30 28 33 36 20 26 21 5 19 33 26 15 24 26 13 19 37 28 35 24 BXD23 M 1 2 040695C "59,283" "±5,565" "59,283" "±5,565" 9.4% "79,711" 74.37 1.072 950106 950406 90 20.5 403 0 3 F20N5f 86 bought from Jax. Fixed by Rob (listed as okay fixation but performance was okay to good). Black color May-14-95 14 "Box 43, Slot L" 3 "12,984 x" 194 x RCS RW and RW "2,991 mm^2" "5,270 mm^2" 31.3 µm^2 0.78% 2550 20 465 23.3 ±9.51 ±2.18 2.0148E-03 3.3986E-08 25 35 33 28 12 21 16 18 23 33 21 33 18 22 4 3 34 29 33 24 BXD24 F 1 2 080994G "62,395" ±992 "±2,429" 7 "65,631" "±4,002" "65,631" "±4,002" 6.1% "104,573" 62.76 1.332 ? 412 940126 940809 195 438 1 3 F113 retired breeder. Mouse from Ben Taylor's colony. Dark brown. 1 necrotic on 0009 Sep-26-94 8 "Box 28, Slot I-1" 4 "13,020 x" 192 x RSC RCS and RW "3,864 mm^2" "5,418 mm^2" 32.0 µm^2 0.52% 3272 17 341 20.1 ±4.89 ±1.22 4.3545E-03 6.6349E-08 75320 61310 81.4% " 14,011 " 14 25 16 16 16 25 18 24 15 21 21 16 20 31 16 27 20 BXD24 aged mouse F 1 2 072994E "65,373" "±4,739" "65,373" "±4,739" 7.2% "142,394" 45.91 1.226 ? 920626 940729 763 NA 413 1 1 Aged BXD from Ben Taylor's colony. Perfused at Jackson Lab by Dan. No pupillary light reflex. Lots of necrosis Sep-16-94 3 "Box 28, Slot A-1" 4 "13,057 x" 144 x RCS RCS and RW "2,943 mm^2" "5,418 mm^2" 31.8 µm^2 0.49% 4480 22 321 14.6 ±4.85 ±1.06 3.0499E-03 4.6653E-08 62531 66393 106.2% " 3,862 " 22 10 4 18 16 11 16 16 10 21 8 10 15 21 20 12 13 15 15 15 11 22 BXD24 aged mouse F 1 2 072994F "61,551" "±4,456" "61,551" "±4,456" 7.2% "125,654" 48.98 -0.347 ? 920626 940729 763 NA 432 1 1 Aged BXD from Ben Taylor's colony. Perfused at Jackson Lab by Dan. Slight pupillary light reflex in left eye only. Lots of necrosis Oct-15-94 4 "Box 28, Slot E-1" 3 "13,028 x" 249 x RCS RCS and RW "7,769 mm^2" "5,418 mm^2" 31.9 µm^2 0.56% 3936 22 344 15.6 ±5.19 ±1.13 3.2476E-03 5.2763E-08 60862 67843 111.5% " 6,982 " 15 19 15 16 15 24 19 5 4 11 19 16 19 19 6 16 14 15 18 18 18 23 BXD24 M 1 2 100494H "62,573" "±2,830" "62,573" "±2,830" 4.5% "55,797" 112.14 0.073 ? 940702 941004 94 19.5 397 0 2 F114. Bought. Brown coat. All negative mislabeled 100494F BXD21 "2 necrotic, negatives 0002, 0016" Dec-20-94 16 "Box 34, Slot L-1" 4 "13,045 x" 193 x TK RW and RW "2,069 mm^2" "5,418 mm^2" 31.8 µm^2 0.97% 1752 17 607 35.7 ±6.46 ±1.61 8.3021E-03 1.3268E-07 60962 66297 108.8% " 5,335 " 40 32 28 21 37 38 41 41 46 33 32 43 44 35 33 30 33 BXD24 M 1 2 100494I "61,535" "±3,002" "61,535" "±3,002" 4.9% "75,169" 81.86 -0.354 ? 940705 941004 91 28.5 409 0 4 F114. Bought. Brown coat. All negative mislabeled 100494F BXD21 none Dec-12-94 19 "Box 30?, Slot Q-2" 4 "13,018 x" 194 x RCS RW and RW "2,817 mm^2" "5,418 mm^2" 32.0 µm^2 0.98% 2351 23 602 26.2 ±5.99 ±1.28 7.1371E-03 1.1598E-07 63924 61865 96.8% " 2,059 " 20 25 31 34 32 35 18 23 25 15 32 29 35 19 29 25 24 24 31 31 16 23 26 BXD24 F 1 2 100494K "61,386" "±4,376" "61,386" "±4,376" 7.1% "78,983" 77.72 -0.415 ? 940702 941004 94 21.3 381 1 2 F114. All negatives mislabeled as A/J. Also poor sampling procedure.Bought. Brown coat. 1 necrotic on 0004 Nov-22-94 1 "Box 20, Slot L-1" 4 "12,952 x" 192 x THK THK and RW "2,927 mm^2" "5,418 mm^2" 32.3 µm^2 0.82% 2446 20 502 25.1 ±7.80 ±1.79 3.3742E-03 5.4967E-08 57701 72191 125.1% " 14,490 " 23 17 34 23 23 32 37 19 37 14 20 31 33 12 23 22 27 17 22 36 BXD24 original F 1 2 100494L "58,714" "±3,093" "57,727" "±5,759" 10.0% "75,978" 75.98 -1.516 ? 940704 941004 92 23.0 416 1 5 F114. All negatives mislabeled as A/J. Also poor sampling procedure.Bought. Brown coat. none 5 or 6 Nov-22-94 4 "Box 20, Slot M-2" 4 "13,002 x" 192 x THK THK and RW "2,803 mm^2" "5,418 mm^2" 32.0 µm^2 0.84% 2371 20 487 24.4 ±10.59 ±2.43 6.4607E-03 1.1004E-07 63170 61886 98.0% " 1,284 " 27 27 28 39 6 4 27 edge 32 27 26 22 7 39 36 26 edge 29 30 6 22 27 BXD24 replicate 0.00001 100494L "59,114" "±3,667" 6.2% "79,326" 74.52 ? none Jan-22-96 4 "Box 20, Slot M-2" 3 "13,034 x" 184 x RW RW and RW "2,697 mm^2" "5,451 mm^2" 32.1 µm^2 0.89% 2472 22 526 23.9 ±6.80 ±1.48 28 30 25 24 28 26 21 28 27 15 24 34 30 20 14 14 13 18 18 20 34 35 BXD24 "arrived July 13, 95" F 950524 7 F116/115 Brown grey mouse BXD24 poor fixation M 100494J "76,691" 940822 941004 43 25.0 416 0 6 F114. Bought. Brown coat. Not possible to count unmyelinated fibers. "Poor fixation probably accounts for low count of about 54,400. Discard nerve data." Jan-17-94 20 "Box 35, Slot M-2" 1 "16,245 x" 193 x RCS RW and RW "2,854 mm^2" "5,418 mm^2" 20.5 µm^2 0.78% 3736 29 422 14.6 ±5.32 ±1.00 21 16 13 19 25 13 11 16 16 16 22 15 13 13 19 9 3 11 9 21 17 20 11 14 2 10 11 19 17 BXD25 F 1 2 112894M "54,759" "±1,418" "±3,171" 6 "54,120" "±3,206" "54,120" "±3,206" 5.9% "65,646" 82.44 -0.202 ? 409 940822 941128 98 22.0 431 1 1 F95 bought afrom JAX. Dark brown coat. none Jan-17-95 5 "Box 36, Slot A-1" 4 "16,139 x" 193 x RCS RW and RW "2,447 mm^2" "5,418 mm^2" 20.8 µm^2 0.86% 3156 27 463 17.1 ±5.18 ±1.02 5.4693E-03 1.0106E-07 53650 54552 101.7% 902 18 10 20 26 15 15 19 13 12 26 15 9 21 14 16 8 13 19 19 30 16 17 14 17 23 18 20 BXD25 F 1 2 112894N "53,875" "±4,923" "53,875" "±4,923" 9.1% "76,514" 70.41 -0.279 ? 940822 941128 98 17.5 410 1 1 F95 bought afrom JAX. Dark brown coat. none Jan-19-95 7 "Box 36, Slot H-4" 4 "16,180 x" 195 x THK RW and RW "2,899 mm^2" "5,418 mm^2" 20.7 µm^2 0.57% 3697 21 306 14.6 ±5.95 ±1.33 2.3342E-03 4.3325E-08 57307 50753 88.6% " 6,554 " 15 21 17 20 4 9 21 21 10 13 13 12 2 19 25 14 17 18 17 10 8 BXD25 M 1 2 120594F "58,753" "±5,125" "58,753" "±5,125" 8.7% "56,347" 104.27 1.259 ? 941015 941205 51 21.0 421 0 2 F95. Bought from Jax. Dark brown. none Jan-19-95 15 "Box 36, Slot F-2" 4 "16,280 x" 195 x THK RW and RW "2,135 mm^2" "5,418 mm^2" 20.4 µm^2 0.69% 2756 19 405 21.3 ±7.89 ±1.86 2.3613E-03 4.0190E-08 57574 59810 103.9% " 2,236 " 27 21 12 4 28 22 24 31 13 21 19 28 25 17 29 23 7 21 33 BXD25 original M 1 2 120594G "58,227" "±3,429" "64,503" "±4,949" 7.7% "87,284" 73.90 1.094 ? 941020 941205 46 18.5 406 0 3 F95. Bought from Jax. Dark brown. Excellent sample. No problems with this estimate. 1 necrotic on 0038 Jan-19-95 16 "Box 36, Slot G-1" 4 "16,216 x" 194 x THK RW and RW "3,296 mm^2" "5,418 mm^2" 20.6 µm^2 0.52% 4236 22 335 15.2 ±5.35 ±1.17 5.1876E-03 8.9091E-08 64470 64086 99.4% 384 22 19 17 14 10 13 10 20 8 19 27 11 9 11 16 19 10 16 15 17 24 8 BXD25 replicate M 0.00001 120594G "52,431" "±4,756" 9.1% "87,853" 59.68 ? Replicate analysis of the same thin-section. "Again, excellent analysis. No problems at all. The difference is truely due to sampling." 4 necrotic Jan-22-96 16 "Box 36, Slot G-1" 4 "12,931 x" 185 x RW RW and RW "3,005 mm^2" "5,451 mm^2" 32.6 µm^2 0.82% 2695 22 428 19.5 ±8.09 ±1.76 #DIV/0! #DIV/0! 25 8 18 33 28 14 19 27 24 28 1 24 21 12 14 6 12 21 27 20 22 24 BXD25 L F 1 2 120594H do not average "53,110" "±2,418" "53,110" "±4,809" 9.1% "70,974" 74.83 -0.520 L 941015 941205 51 14.3 401 1 2 F95. Huge asymmetry in diameter of optic nerves. Right nerve (see below) is very small. Left nerve even bigger than normal. Bought from Jax. Dark brown coat. none Jan-19-95 18 "Box 36, Slot I-3" 3 "12,941 x" 193 x THK RW and RW "2,648 mm^2" "5,418 mm^2" 32.4 µm^2 0.87% 2194 19 460 24.2 ±9.30 ±2.19 9.5865E-03 1.8050E-07 55717 50209 90.1% " 5,508 " 22 6 40 23 20 28 23 26 16 23 6 28 35 37 edge 22 26 38 18 23 BXD25 "R, other side" 120594H other side "10,941" ±514 4.7% "12,903" 84.80 R dup Other side done by Stacie. lots of necrotic fibers (about 8 in this sample) Jul-11-95 2 "Box 44,Slot A-1" 4 "12,706 x" 241 x SS SS and SS and RW 747 mm^2 "5,270 mm^2" 32.6 µm^2 5.57% 395 22 609 27.7 ±5.96 ±1.30 35 34 26 23 24 29 15 36 34 29 23 31 25 24 27 38 20 32 20 33 24 27 BXD25 M 1 2 072495H-2 "50,469" "±4,757" "50,469" "±4,757" 9.4% "74,269" 67.96 -1.353 ? 950617 950724 37 15.0 406 0 4 "F97, perfused by Dan at JAX. Brown coat." none Dec-10-95 7 "Box 55, Slot T-1" 3 "12,987 x" 192 x RCS RW and RW "2,744 mm^2" "5,225 mm^2" 31.0 µm^2 0.83% 2398 20 421 21.1 ±8.65 ±1.98 2.3479E-03 4.6522E-08 17 14 26 18 17 8 14 11 16 31 36 33 19 27 13 13 38 21 27 22 BXD25 M 072495I-2 950617 950724 37 16.2 390 4 "F97, perfused by Dan at JAX. Brown coat." BXD25 ordered Oct 94 M BXD27 M 1 2 040695D "51,568" "±1,391" "±3,110" 6 "52,568" "±3,137" "52,568" "±3,137" 6.0% "49,939" 105.26 0.321 ? 365 950227 950406 38 16.5 369 352 41 0 1 F115. From Jax "Negatives may be mislabeled as case G, but his is really case D. Perfused by Rob and Richelle. Chocolate color" 1 necrotic fiber Apr-28-95 17 "Box 43, Slot E-1" 3 "12,855 x" 248 x RW RW and RW "3,076 mm^2" "5,270 mm^2" 31.9 µm^2 1.34% 1566 21 705 33.6 ±8.96 ±2.00 5.6099E-03 1.0672E-07 26 35 36 27 29 31 39 28 39 43 33 30 35 27 58 42 31 29 46 25 16 BXD27 brain to Allman M 1 2 040695E "55,662" "±5,123" "55,662" "±5,123" 9.2% "49,133" 113.29 1.316 ? 950227 950406 38 18.3 384 364 42 0 1 F115. From Jax "Negatives may be mislabeled, but this is case E. Perfused by Rob and Richelle. Chocolate color" Apr-28-95 18 "Box 43, Slot F-2" 3 "12,855 x" 248 x RW RW and RW "3,026 mm^2" "5,270 mm^2" 31.9 µm^2 1.49% 1541 23 831 36.1 ±15.60 ±3.33 2.2170E-03 3.9830E-08 38 29 25 30 36 47 46 64 12 27 57 56 34 24 2 27 27 19 41 62 48 38 42 BXD27 M 1 2 040695F "53,321" "±3,965" "53,321" "±3,965" 7.4% "63,957" 83.37 0.564 ? 950227 950406 38 16.0 375 357 42 0 1 F115. From Jax "Perfused by Rob and Richelle. Rated perfusion as good, but actual performance was only marginal. Chocolate color" 1 or 2 necrotic May-12-95 20 "Box 43, Slot M-2" 2 "12,934 x" 193 x RW RW and RW "2,383 mm^2" "5,270 mm^2" 31.5 µm^2 0.94% 2030 19 499 26.3 ±8.29 ±1.95 3.5802E-03 6.7144E-08 34 19 30 29 24 13 36 26 17 21 22 22 22 22 27 31 51 28 25 BXD27 brain to Allman F 1 2 040695G "52,080" "±1,882" "52,080" "±1,882" 3.6% "59,979" 86.83 0.165 ? 950227 950406 38 14.8 368 348 45 1 1 F115. From Jax "Perfused by Rob and Richelle. Perfusion rated great, but performance was only good. Chocolate color" none May-12-95 1 "Box 43, Slot N-1" 3 "12,864 x" 248 x RW RW and RW "3,684 mm^2" "5,270 mm^2" 31.8 µm^2 1.06% 1884 20 553 27.7 ±4.36 ±1.00 1.5472E-02 2.9708E-07 30 31 25 35 24 22 26 24 31 19 27 31 25 22 34 31 28 26 33 29 BXD27 hydrocephaly; Get replacement animal F 040695H "36,489" "±2,674" 7.3% "51,145" 71.34 ? 950227 950406 38 13.0 see notes see notes see notes 1 1 F115. From Jax. Hydrocephalic case with brain weight of 310.1 mg. No cerebellum present "Perfused by Rob and Richelle. Perfusion rated as great, but performance was only marginal. Chocolate color" none May-12-95 4 "Box 43, Slot O-2" 2 "12,956 x" 248 x RW RW and RW "3,150 mm^2" "5,270 mm^2" 31.4 µm^2 1.23% 1629 20 448 22.4 ±7.16 ±1.64 25 25 30 19 27 13 20 18 32 22 28 26 24 7 36 16 16 24 27 13 BXD27 F 1 2 040695I "48,194" "±2,547" "48,194" "±2,547" 5.3% "46,569" 103.49 -1.085 ? 950227 950406 38 13.1 350 337 40 1 1 F115. From Jax Perfused by Rob and Richelle. Chocolate color none May-14-95 5 "Box 43, Slot P-1" 3 "12,817 x" 247 x RW RW and RW "2,851 mm^2" "5,270 mm^2" 32.1 µm^2 1.38% 1452 20 664 33.2 ±7.65 ±1.75 7.8212E-03 1.6228E-07 23 42 34 41 33 31 28 35 40 26 29 31 18 27 31 35 44 48 41 27 BXD27 F 1 2 072195A "47,583" "±3,484" "47,583" "±3,484" 7.3% "80,463" 59.14 -1.281 ? 950523 950721 59 16.8 343 1 1 Get generation. This animal was a replacement for the hydrocephalic casee Brown coat Oct-19-95 1 "Box 55, Slot I-1" 4 "12,933 x" 192 x RCS RW and RW "2,952 mm^2" "5,329 mm^2" 31.9 µm^2 0.75% 2525 19 358 18.8 ±5.85 ±1.38 4.1389E-03 8.6984E-08 9 13 21 14 21 17 14 23 19 21 22 8 26 31 18 24 19 24 14 BXD28 F 1 2 013095S "53,864" "±3,624" "±7,249" 5 "60,534" "±3,200" "60,534" "±3,200" 5.3% "67,107" 90.21 0.920 ? 405 941216 950130 45 14.8 401 1 1 F59. Bought from Jax. Brown coat 2 necrotic Feb-23-95 6 "Box 40, Slot B-2" 4 "13,165 x" 192 x RCS RW and RW "2,484 mm^2" "5,418 mm^2" 31.3 µm^2 1.16% 2147 25 705 28.2 ±7.30 ±1.49 6.1580E-03 1.0173E-07 60461 60598 100.2% 138 30 29 29 23 24 22 32 28 23 24 26 34 22 32 26 16 31 33 32 52 25 28 27 17 40 BXD28 F 1 2 013095T "47,861" "±3,429" "47,861" "±3,429" 7.2% "54,607" 87.65 -0.828 ? 941216 950130 45 16.0 407 1 1 F59. Bought from Jax. Brown coat. Tissue is not very nice looking tissue. But no trouble in counting. none Feb-23-95 8 "Box 40, Slot A-1" 2 "13,165 x" 192 x RCS RW and RW "2,022 mm^2" "5,418 mm^2" 31.3 µm^2 1.14% 1747 20 548 27.4 ±8.56 ±1.96 4.2859E-03 8.9550E-08 52400 43318 82.7% " 9,083 " 34 28 23 31 24 22 5 29 25 30 19 18 33 37 24 24 29 34 32 47 BXD28 Get info M 1 2 021795D "44,367" "±2,619" "44,367" "±2,619" 5.9% "60,339" 73.53 -1.310 ? 941227 950217 52 21.8 414 0 2 F59. Bought from Jax. Very large axons. Extremely few unmyelinated fibers. Brown mouse Mar-29-95 12 "Box 40, Slot T-1" 4 "13,089 x" 190 x RW RW and RW "2,187 mm^2" "5,270 mm^2" 30.8 µm^2 1.07% 1961 21 475 22.6 ±5.97 ±1.34 6.7933E-03 1.5312E-07 52400 43318 82.7% " 9,083 " 24 26 24 31 17 23 16 8 23 10 29 30 21 27 20 26 24 20 25 28 23 BXD28 M 1 2 021795E "58,751" "±3,043" "58,751" "±3,043" 5.2% "76,042" 77.26 0.674 ? 941227 950217 52 23.0 429 0 2 "F59. Bought from Jax. Ist estimate of Mar 30, 1995 is not valid because section is incomplete. This sample is good." Complete section this time. none Jan-22-96 13 "Box 40, Slot R-7" 4 "13,029 x" 185 x RW RW and RW "2,593 mm^2" "5,263 mm^2" 31.0 µm^2 0.90% 2453 22 527 24.0 ±5.69 ±1.24 6.6460E-03 1.1312E-07 30 30 28 13 31 22 20 29 21 24 29 30 21 21 21 19 20 36 21 24 15 22 BXD28 Get info from Coleen on birth date F 1 2 052995G "57,809" "±4,917" "57,809" "±4,917" 8.5% "68,166" 84.81 0.544 ? 950320 950529 70 18.8 404 1 3 "Shipping error from Jax. Both BXD5 and BXD28 were to be shipped, but the BXD28 order was erroneously canceled by phone. In the end, the BXD28s were sent instead of the BXD5s. We called Coleen Towser. " "Brown, redish brown (auburn) coat" 2 necrotic Oct-8-95 9 "Box 48, Slot L-1" 4 "12,948 x" 191 x RCS RCS and RW "2,483 mm^2" "5,329 mm^2" 31.8 µm^2 1.12% 2144 24 647 27.0 ±11.00 ±2.29 2.4954E-03 4.3166E-08 27 40 38 10 32 23 25 25 6 31 23 21 24 28 45 30 22 13 10 48 21 31 44 30 BXD28 Get info from Coleen on birth date F 052995H 950320 950529 70 19.3 377 3 "Shipping error from Jax. Both BXD5 and BXD 28 were to be shipped, but the BXD28 order was erroneously canceled by phone. In the end the BXD28s were sent instead of the BXD5s. We called Coleen Towser" "Brown, redish brown (auburn) coat" BXD29 original M 1 2 012795K "63,539" "±1,859" "±4,156" 6 "69,862" "±6,201" "69,862" "±6,201" 8.9% "111,592" 62.60 1.521 ? 398 941010 950127 109 20.8 412 0 1 F116. Bought from Jax. Grey 1 necrotic Feb-23-95 21 "Box 40, Slot C-1" 4 "13,165 x" 192 x RCS RCS and RW "4,131 mm^2" "5,418 mm^2" 31.3 µm^2 0.59% 3570 21 411 19.6 ±7.77 ±1.74 1.9076E-03 2.7306E-08 77230 61752 80.0% " 15,479 " 24 13 21 29 14 31 31 21 24 19 11 16 11 21 17 15 19 15 14 7 38 BXD29 replicate 0.00001 012795K "66,401" "±5,189" 7.8% "109,044" 60.89 ? Repeat of different section none Feb-1-96 3 "13,165 x" 192 x RW RW and RW "4,037 mm^2" "5,396 mm^2" 31.1 µm^2 0.69% 3502 24 455 19.0 ±7.10 ±1.48 28 13 22 20 12 23 18 9 7 23 21 22 14 25 23 30 26 24 16 1 16 15 27 20 BXD29 M 1 2 012795L "65,346" "±5,342" "65,346" "±5,342" 8.2% "108,296" 60.34 0.435 ? 941118 950127 70 22.0 398 0 2 F116. Bought from Jax. none Feb-23-95 2 "Box 40, Slot E-1" 4 "13,165 x" 192 x RCS RCS and RW "4,009 mm^2" "5,418 mm^2" 31.3 µm^2 0.64% 3464 22 415 18.9 ±7.07 ±1.54 2.3992E-03 3.6715E-08 72115 58574 81.2% " 13,541 " 10 28 31 13 11 18 22 16 10 17 19 17 27 19 33 12 18 19 25 21 23 6 BXD29 M 1 2 012795M "61,989" "±3,699" "61,989" "±3,699" 6.0% "87,186" 71.10 -0.373 ? 941206 950127 52 26.5 412 0 3 F117. Bought from Jax. Marginal fixation. Unmyelinated fibers may not be preserved. none Feb-23-95 3 "Box 40, Slot D-2" 2 "13,165 x" 192 x RCS RCS and RW "3,228 mm^2" "5,418 mm^2" 31.3 µm^2 0.79% 2789 22 489 22.2 ±6.08 ±1.33 4.7458E-03 7.6559E-08 61354 62621 102.1% " 1,268 " 15 17 27 22 22 27 25 8 13 28 19 26 23 21 23 18 32 28 31 28 17 19 BXD29 original F 1 2 021795A "58,132" "±3,188" "58,132" "±3,188" 5.5% "85,984" 67.61 -1.301 ? 941227 950217 52 13.0 366 1 4 F116. Bought from Jax. Grey mice Apr-10-95 6 "Box 41, Slot L-1" 4 "10,880 x" 194 x RCS RW and RW "3,235 mm^2" "5,270 mm^2" 44.5 µm^2 1.04% 1931 20 602 30.1 ±7.20 ±1.65 6.0199E-03 1.0356E-07 35 26 28 26 30 29 33 40 12 25 37 24 24 34 38 25 44 31 35 26 BXD29 replicate 0.00001 021795A "82,467" "±4,832" 5.9% "109,044" 75.63 ? Repeat of a different section none Feb-1-96 3 "13,165 x" 192 x RW RW and RW "4,037 mm^2" "5,396 mm^2" 31.1 µm^2 0.63% 3502 22 518 23.5 ±6.32 ±1.38 28 26 18 11 18 30 36 20 23 16 24 25 28 19 33 23 26 28 12 21 24 29 BXD29 F 1 2 021795B "65,278" "±4,336" "65,278" "±4,336" 6.6% "80,505" 81.09 0.418 ? 941227 950217 52 15.3 389 1 4 F116. Bought from Jax. Grey mice Apr-10-95 8 "Box 41, Slot M-2" 4 "10,880 x" 194 x RCS RW and RW "3,029 mm^2" "5,270 mm^2" 44.5 µm^2 1.11% 1808 20 722 36.1 ±10.45 ±2.40 3.6547E-03 5.5986E-08 35 14 37 31 32 50 53 43 50 43 25 33 41 duplicate 40 39 33 45 36 26 16 BXD29 M 1 2 021795C "60,629" "±3,780" "60,629" "±3,780" 6.2% "72,415" 83.72 -0.700 ? 941227 950217 52 20.8 409 0 4 F116. Bought from Jax. Grey mice 2 necrotic Apr-20-95 9 "Box 41, Slot T-1" 3 "12,975 x" 193 x RCS RW and RW "2,695 mm^2" "5,270 mm^2" 31.3 µm^2 0.82% 2313 19 498 26.2 ±6.93 ±1.63 4.4792E-03 7.3879E-08 29 14 29 35 25 34 29 23 12 25 20 30 20 33 22 25 38 32 23 BXD30 M 1 2 072195B "68,079" "±2,154" "±4,816" 6 "66,877" "±6,452" "66,877" "±6,452" 9.6% "86,580" 77.24 0.803 ? 377 950528 950721 54 18.8 383 0 1 Get Generation. Check strain DBA coloration 1 necrotic Oct-27-95 2 "Box 55, Slot J-2" 4 "12,909 x" 192 x RCS RW and RW "3,204 mm^2" "5,329 mm^2" 32.0 µm^2 0.74% 2708 20 494 24.7 ±10.39 ±2.38 1.6908E-03 2.5283E-08 15 16 16 43 43 12 24 31 32 11 37 31 18 41 19 18 27 21 24 15 BXD30 M 1 2 072195C "63,727" "±4,480" "63,727" "±4,480" 7.0% "93,243" 68.34 0.045 ? 950528 950721 54 20.5 381 0 1 Get Generation. Check strain DBA coloration none Oct-19-95 21 "Box 55, Slot H-2" 4 "12,955 x" 191 x RCS RW and RW "3,402 mm^2" "5,329 mm^2" 31.8 µm^2 0.68% 2937 20 434 21.7 ±6.65 ±1.53 3.3420E-03 5.2442E-08 37 23 11 24 13 19 26 15 20 14 27 20 18 29 18 23 20 30 17 30 BXD30 F 1 2 072195D "72,960" "±5,562" "72,960" "±5,562" 7.6% "88,954" 82.02 2.267 ? 950528 950721 54 16.0 361 1 1 Get Generation. Check strain DBA coloration none Oct-19-95 20 "Box 55, Slot G-1" 4 "12,836 x" 191 x RCS RW and RW "3,241 mm^2" "5,329 mm^2" 32.3 µm^2 0.69% 2750 19 504 26.5 ±8.58 ±2.02 2.4898E-03 3.4126E-08 16 22 24 19 26 16 40 29 35 33 47 26 35 19 22 30 25 25 15 BXD30 M 1 2 071795I-2 "61,398" "±5,188" "61,398" "±5,188" 8.4% "98,369" 62.42 -0.515 ? 950502 950717 76 21.6 384 0 2 F95. Perfused by Dan at JAX. He listed color as light brown. none Dec-20-95 4 "Box 56, Slot M-2" 4 "12,962 x" 191 x RCS RW and RW "3,579 mm^2" "5,270 mm^2" 31.4 µm^2 0.61% 3136 19 372 19.6 ±7.02 ±1.65 2.4083E-03 3.9224E-08 31 15 10 22 11 28 31 21 17 26 12 14 26 11 15 15 24 17 26 BXD30 M 1 2 071795R-2 "71,565" "±5,574" "71,565" "±5,574" 7.8% "92,183" 77.63 1.931 ? 950529 950717 49 22.0 391 0 3 F96. Perfused by Dan at JAX. Light brown coat. Good perfusion according to Dan's notes. none Oct-27-95 21 "Box 55, Slot O-2" 4 "12,765 x" 191 x RCS RW and RW "3,378 mm^2" "5,329 mm^2" 32.7 µm^2 0.64% 2819 18 457 25.4 ±8.15 ±1.98 2.4385E-03 3.4074E-08 13 25 22 38 40 29 24 23 39 15 32 25 21 28 24 14 28 17 BXD30 F 071795S-2 950529 950717 49 14.0 354 3 F96. Perfused by Dan at JAX. Light brown coat. Poor fixation according to Dan's perfusion notes. BXD30 F 1 2 071795T-2 "71,947" "±4,647" "71,947" "±4,647" 6.5% "72,523" 99.21 2.023 ? 950529 950717 49 16.0 386 1 3 F96. Perfused by Dan at JAX. Light brown coat. OK perfusion according to Dan's notes. none Oct-27-95 4 "Box 55, Slot P-1" 4 "12,880 x" 192 x RCS RW and RW "2,687 mm^2" "5,329 mm^2" 32.1 µm^2 1.02% 2258 23 733 31.9 ±9.65 ±2.06 3.4834E-03 4.8416E-08 26 27 18 22 28 43 23 28 24 40 32 30 51 30 32 32 41 38 54 16 41 28 29 BXD31 M 1 2 013095K "66,833" "±2,038" "±4,556" 6 "70,494" "±5,099" "70,494" "±5,099" 7.2% "100,677" 70.02 0.804 ? 415 941211 950130 50 23.8 416 0 1 F84. Bought from Jax. Black coat. On 9% NIH fat. none Mar-29-95 10 "Box 40, Slot G-7" 3 "13,143 x" 192 x RW RW and RW "3,699 mm^2" "5,270 mm^2" 30.5 µm^2 0.67% 3300 22 470 21.4 ±7.08 ±1.55 2.8403E-03 4.0292E-08 5 8 21 20 27 13 14 28 25 22 36 19 22 30 25 20 29 19 20 25 22 20 BXD31 M 1 2 013095L "66,259" "±3,258" "66,259" "±3,258" 4.9% "69,863" 94.84 -0.126 ? 941211 950130 50 22.5 427 0 1 F84. Bought from Jax. Black coat none Mar-8-95 12 "Box 40, Slot P-1" 3 "13,050 x" 193 x TK RW and RW "2,614 mm^2" "5,418 mm^2" 31.8 µm^2 1.05% 2196 23 694 30.2 ±6.96 ±1.48 6.5268E-03 9.8504E-08 46 26 28 25 14 26 33 35 22 36 42 31 29 36 37 28 22 25 27 35 30 30 31 BXD31 M 1 2 013095M "71,842" "±5,706" "71,842" "±5,706" 7.9% "104,337" 68.86 1.099 ? 941211 950130 50 22.8 416 51 0 1 F84. Bought from Jax. Black coat. Cerebellum dissected by Rob. 51.2 mg. Mar-29-95 15 "Box 40, SLot F-8" 3 "13,305 x" 192 x RW RW and RW "3,828 mm^2" "5,270 mm^2" 29.8 µm^2 0.57% 3504 20 410 20.5 ±7.10 ±1.63 2.3227E-03 3.2331E-08 26 23 15 20 28 33 18 20 30 21 15 9 14 23 26 11 33 14 16 15 BXD31 M 1 2 013095N "68,392" "±4,585" "68,392" "±4,585" 6.7% "102,587" 66.67 0.342 ? 941211 950130 50 24.3 416 0 1 F84. Bought from Jax. Black coat none Mar-8-95 16 "Box 40, Slot O-2" 2 "13,050 x" 193 x TK RW and RW "3,839 mm^2" "5,418 mm^2" 31.8 µm^2 0.59% 3224 19 403 21.2 ±6.03 ±1.42 3.4338E-03 5.0208E-08 19 27 20 22 12 30 21 6 26 24 26 25 14 25 22 18 26 15 25 BXD31 original F 1 2 013095I "59,184" "±2,219" "61,423" "±3,067" 5.0% "89,942" 68.29 ? 941125 950130 66 19.5 429 1 2 F83. Bought from Jax. "Black coat. 13 poor micrograhs with 33 unmyelinated fibers, 16 good micrographs with 50 unmyelinated fibers total." 1 necrotic fiber on 0003 Feb-19-95 6 "Box 38, Slot S-2" 2 "13,092 x" 194 x RCS RCS and RW "3,390 mm^2" "5,418 mm^2" 31.6 µm^2 1.02% 2845 29 626 21.6 ±5.70 ±1.08 65065 55079 84.7% " 9,986 " 23 25 20 19 29 24 26 19 13 26 16 25 12 19 24 23 22 13 19 27 32 26 21 11 13 20 28 20 31 BXD31 cut other side: replicate 0.00001 013095I "56,723" "±3,215" 5.7% "92,161" 61.55 The 12 edge micrographs are fine but the core of this block is poorly preserved. Low count looks solid to RW. Jan-23-96 6 "Box 38, Slot S-2" 2 "12,980 x" 185 x RW RW and RW "3,166 mm^2" "5,263 mm^2" 31.2 µm^2 0.75% 2950 22 423 19.2 ±4.99 ±1.09 17 18 22 30 13 15 25 25 27 18 16 24 18 10 17 16 15 13 22 20 20 22 BXD31 F 1 2 072195E "64,829" "±3,449" "64,829" "±3,449" 5.3% "79,177" 81.88 -0.440 ? 950608 950721 43 15.0 386 1 3 F85 Black coat 1 necrotic Jan-5-96 19 "Box 55, Slot F-2" 3 "12,970 x" 244 x RW RW and RW "4,711 mm^2" "5,263 mm^2" 31.3 µm^2 0.83% 2530 21 538 25.6 ±6.09 ±1.36 5.7233E-03 8.8284E-08 40 19 26 25 30 34 31 20 24 26 27 20 24 31 25 33 28 17 17 18 23 BXD31 runt F 1 2 013095J "59,818" "±5,984" 10.0% "70,095" 85.34 ? 941211 950130 50 see notes see notes 1 1 F84. Bought from Jax. Looks dehydrated. Humped up like a weaver mutant. Listed in book as a 6.8 gm mouse with a 341 mg brain. Black coat none Feb-21-95 9 "Box 38, Slot T-1" 3 "13,086 x" 193 x TK RW and RW "2,611 mm^2" "5,418 mm^2" 31.6 µm^2 0.86% 2215 19 513 27.0 ±11.46 ±2.70 57157 62770 109.8% " 5,612 " 18 37 27 edge 21 47 30 28 37 27 29 32 20 52 27 16 30 5 19 11 BXD32 F 1 2 013095C "76,836" "±3,355" "±7,502" 6 "72,044" "±4,539" "72,044" "±4,539" 6.3% "77,943" 92.43 -0.639 ? 429 941214 950130 47 16.5 416 1 1 F75. Bought from Jax. Black coat none Mar-3-95 15 "Box 40, Slot L-1" 3 "13,115 x" 195 x RW RCS and RW "2,956 mm^2" "5,418 mm^2" 31.5 µm^2 1.05% 2474 26 757 29.1 ±9.17 ±1.83 3.6374E-03 5.0489E-08 70198 74993 106.8% " 4,795 " 40 35 35 36 42 34 26 38 32 29 35 32 34 31 8 11 36 24 35 26 12 19 33 17 34 23 BXD32 original F 1 2 013095D "85,296" "±4,080" "89,810" "±4,915" 5.5% "68,529" 131.05 1.128 ? 941214 950130 47 17.0 426 1 1 F75. Bought from Jax. No problems. This is really a super high mouse. Black coat none Mar-2-95 17 "Box 40, Slot H-2" 3 "13,061 x" 194 x RCS RCS and RW "2,571 mm^2" "5,418 mm^2" 31.8 µm^2 0.74% 2158 16 666 41.6 ±8.82 ±2.28 5.4654E-03 6.4076E-08 86571 93043 107.5% " 6,473 " 53 35 41 44 34 30 42 45 43 21 45 51 53 48 34 47 BXD32 replicate 0.00001 013095D "75,294" "±7,316" 9.7% "68,660" 109.66 Replicate by Toya to confirm high count of different thin section. none Mar-21-95 17 "Box 40, Slot H-2" "13,085 x" 193 x TK RW and RW "2,568 mm^2" "5,418 mm^2" 31.6 µm^2 0.92% 2170 20 694 34.7 ±14.70 ±3.37 40 36 32 44 12 0 18 14 23 47 36 39 38 54 51 44 27 48 50 41 BXD32 original M 1 2 013095F "72,946" "±3,961" "68,887" "±5,509" 8.0% "97,143" 70.91 -0.518 ? 941214 950130 47 21.0 426 0 1 F75. Bought from Jax. Could be a slight undercount due to marginal fixation of unmyelinated fibers. Black coat none Mar-8-95 12 "Box 40, Slot N-1" 2 "13,054 x" 194 x RW RW and RW "3,640 mm^2" "5,418 mm^2" 31.8 µm^2 0.72% 3055 22 496 22.5 ±8.26 ±1.80 4.8717E-03 6.6784E-08 75497 61669 81.7% " 13,827 " 18 32 31 30 29 20 10 33 12 24 16 18 22 6 21 28 17 22 21 41 26 19 BXD32 replicate 0.00001 013095F "77,290" "±5,698" 7.4% "96,235" 80.31 Replicate by Toya to confirm low count of different thin section 1 necrotic cell or fiber Mar-21-95 12 "Box 40, Slot N-1" "13,052 x" 193 x TK RW and RW "3,599 mm^2" "5,418 mm^2" 31.8 µm^2 0.79% 3026 24 613 25.5 ±9.03 ±1.88 26 33 20 21 13 25 20 33 35 27 21 27 22 26 22 22 25 20 39 14 53 30 29 10 BXD32 M 1 2 013095G "77,917" "±5,311" "77,917" "±5,311" 6.8% "89,395" 87.16 0.144 ? 941214 950130 47 21.0 434 0 1 F75. Bought from Jax. Black coat none Feb-19-95 3 "Box 38, Slot Q-2" 3 "13,092 x" 194 x RCS TK and RW "3,370 mm^2" "5,418 mm^2" 31.6 µm^2 0.71% 2828 20 551 27.6 ±8.19 ±1.88 2.9079E-03 3.7321E-08 84278 71551 84.9% " 12,727 " 14 20 18 37 37 25 19 29 15 21 35 36 29 31 29 22 30 44 27 33 BXD32 M 1 2 013095H "85,632" "±4,780" "85,632" "±4,780" 5.6% "101,283" 84.55 1.172 ? 941214 950130 47 22.5 445 0 1 F75. Bought from Jax. Black coat none Feb-21-95 5 "Box 38, Slot R-1" 3 "13,086 x" 194 x TK TK and RW "3,795 mm^2" "5,418 mm^2" 31.6 µm^2 0.87% 3201 28 749 26.8 ±7.76 ±1.49 3.8870E-03 4.5391E-08 84372 86887 103.0% " 2,515 " 18 22 37 31 37 27 22 25 22 22 23 30 30 30 35 5 21 35 23 34 26 18 28 29 42 28 16 33 BXD32 M 1 2 061495I "67,181" "±4,509" "67,181" "±4,509" 6.7% "65,955" 101.86 -1.287 ? 950418 950614 57 21.5 401 0 2 Case #297 none #233 #234 Jan-5-96 12 "Box 48, Slot M-2" 4 "13,046 x" 245 x RW RW and RW "3,946 mm^2" "5,263 mm^2" 30.9 µm^2 1.03% 2133 22 693 31.5 ±9.69 ±2.11 3.4611E-03 5.1519E-08 40 32 33 38 30 30 23 11 24 52 33 40 45 47 20 26 29 28 30 35 29 18 BXD32 F 081595EE 950418 950815 119 35.0 410 2 Part of cerebellum missing from brain. Weight is probably 10-20 mg too low. Case #298 Mated to CAST/Ei male #285 Tissue for DNA #233 #234 BXD32 F 081595GG 950512 950815 95 21.5 456 4 Used to make F1s. Born in colony Case #255 Tissue for DNA #233 #234 BXD32 F 081595HH 950512 950815 95 26.0 458 4 Used to make F1s. Born in colony Case #256 Tissue for DNA #233 #234 BXD32 F 091895S 950603 950918 107 6 "7 in litter, 4 taken for P0 study of Richelle's. " Case #272 #233 #234 BXD32 M 121995J 950224 951219 298 28.5 440 3 F76. Bought from Jax. Case #234. Mated to female 233 below. Black coat BXD32 M 121995K 950224 951219 298 33.0 464 3 F76. Born in colony. Case #257 BXD32 M 121995L 950224 951219 298 27.5 445 3 F76. Born in our colony Case #270 or #271 BXD32 F 121995M 950224 951219 298 30.0 452 3 Bought from Jax "This is Case #233. She had a litter of 7 BXD32 by her brother May 12, 1995. 4 of these P0 perfused for RCS's developmental study. Black coat" BXD32 M 010496C 950625 960104 193 27.0 453 7 "Five born in this litter, 3 used for P0 study." Case #301 #233 #234 BXD32 F 010496D 951127 960104 38 19.9 433 5 Born in our colony Non-agouti #272 #270 or #301 or #302 BXD32 M 010496E 951127 960104 38 21.5 426 5 Born in our colony Non-agouti #233? #270 or #301 or #302 BXD32 F 010496F 951127 960104 38 16.0 403 5 Born in our colony Non-agouti #233? #270 or #301 or #302 BXD32 poor fix M 013095E "64,902" 941214 950130 47 22.5 434 1 F75. Bought from Jax. Terrible fixation. Cannot count accurately. Estimate is way low. Black coat none Mar-3-95 19 "Box 40, Slot M-2" 1 "16,246 x" 195 x RW RCS "2,461 mm^2" "5,418 mm^2" 20.5 µm^2 0.57% 3162 18 373 20.7 ±7.53 ±1.83 19 34 17 5 17 20 22 23 25 28 23 12 26 13 16 14 25 34 BXH2 F 1 2 072595C-2 "63,406" "±4,397" "±4,397" 2 "60,297" "±4,298" "60,297" "±4,298" 7.1% "81,568" 73.92 950613 950725 42 18.6 415 1 1 F99. Perfused by Dan at JAX. Black coat. none Jan-24-96 5 "Box 58, Slot R-1" 4 "12,625 x" 186 x RW RW and RW "2,810 mm^2" "5,263 mm^2" 33.0 µm^2 0.89% 2470 22 537 24.4 ±7.97 ±1.74 3.4187E-03 5.6698E-08 26 20 26 28 26 17 37 19 21 29 16 35 30 33 28 31 23 6 36 13 19 18 BXH2 F 1 2 012595D-2 "66,516" "±4,061" "66,516" "±4,061" 6.1% "79,988" 83.16 950613 950725 42 19.0 414 1 1 F99. Perfused by Dan at JAX. Black coat. Black coat. none Jan-24-96 8 "Box 58, Slot T-1" 4 "12,963 x" 185 x RW RW and RW "2,752 mm^2" "5,263 mm^2" 31.3 µm^2 0.86% 2554 22 573 26.0 ±7.29 ±1.59 4.2259E-03 6.3533E-08 38 28 20 23 36 34 30 25 29 9 28 34 26 20 20 13 20 21 28 30 28 33 BXH3 F 1 2 071795M-2 "59,642" #DIV/0! #DIV/0! 1 "59,642" "±3,368" "59,642" "±3,368" 5.6% "70,744" 84.31 ? 950531 950717 47 21.6 472 1 1 F106. Perfused by Dan at JAX. Agouti coat. none 11 "Box 56, Slot T-1" 4 "13,098 x" 190 x RW RW and RW "2,548 mm^2" "5,263 mm^2" 30.7 µm^2 0.95% 2306 22 569 25.9 ±6.69 ±1.46 5.5090E-03 9.2367E-08 29 31 20 24 39 23 31 29 19 17 24 38 18 28 28 12 19 26 33 25 27 29 BXH3 F 011795N-2 950531 950717 47 18.2 450 1 F106. Perfused by Dan at JAX. Agouti coat. 4 "13,131 x" 189 x RW RW and RW "3,136 mm^2" "5,263 mm^2" 30.5 µm^2 #DIV/0! 0 0 0 #DIV/0! #DIV/0! #DIV/0! #DIV/0! #DIV/0! BXH4 F 071895A-2 "68,666" "±2,282" "±3,227" 3 950606 950718 42 20.0 472 1 F100. Perfused by Dan at JAX. 4 "13,131 x" 189 x RW RW and RW "3,136 mm^2" "5,263 mm^2" 30.5 µm^2 #DIV/0! 0 0 0 #DIV/0! #DIV/0! #DIV/0! #DIV/0! #DIV/0! BXH4 F 1 2 071895B-2 "65,719" "±4,022" "65,719" "±4,022" 6.1% "63,748" 103.09 -0.913 ? 950513 950718 66 17.6 446 1 2 F100. Perfused by Dan at JAX. none Jan-12-96 7 "Box 57, Slot A-1" 4 "12,925 x" 245 x RW RW and RW "3,827 mm^2" "5,263 mm^2" 31.5 µm^2 1.14% 2023 23 747 32.5 ±9.32 ±1.99 4.2483E-03 6.4644E-08 31 33 38 29 50 33 30 43 38 10 27 14 39 38 25 40 25 46 35 33 23 31 36 BXH4 F 1 2 071895C-2 "68,165" "±4,344" "68,165" "±4,344" 6.4% "87,693" 77.73 -0.155 ? 950513 950718 66 23.2 501 2 F100. Perfused by Dan at JAX. none Jan-15-96 9 "Box 57, Slot B-2" 4 "13,131 x" 189 x RW RW and RW "3,136 mm^2" "5,263 mm^2" 30.5 µm^2 0.77% 2873 22 522 23.7 ±6.93 ±1.51 3.7841E-03 5.5513E-08 12 20 38 30 20 28 31 13 23 34 28 12 19 18 29 26 17 25 27 25 25 22 BXH4 F 1 2 071895D-2 "72,115" "±5,298" "72,115" "±5,298" 7.3% "90,123" 80.02 1.069 ? 950513 950718 66 24.0 495 1 2 F100. Perfused by Dan at JAX. none Jan-15-96 11 "Box 57, Slot C-1" 4 "12,968 x" 191 x RW RW and RW "3,283 mm^2" "5,263 mm^2" 31.3 µm^2 0.76% 2879 22 551 25.0 ±8.43 ±1.84 2.6917E-03 3.7325E-08 22 21 27 34 31 33 28 21 27 32 20 28 39 37 32 19 15 19 19 10 7 30 BXH6 F 1 2 071795L-2 "51,926" "±1,627" "±2,818" 4 "52,336" "±3,787" "52,336" "±3,787" 7.2% "69,340" 75.48 0.146 ? 950521 950717 57 15.5 430 1 1 F118. Perfused by Dan at JAX. Agouti coat color. none Jan-15-96 9 "Box 56, Slot S-2" 4 "13,027 x" 244 x RW RW and RW "4,117 mm^2" "5,263 mm^2" 31.0 µm^2 0.98% 2236 22 515 23.4 ±7.76 ±1.69 3.8231E-03 7.3049E-08 26 17 28 30 26 10 15 26 31 21 27 25 28 36 32 15 32 6 16 17 27 24 BXH6 F 1 2 071995E-2 "50,465" "±4,240" "50,465" "±4,240" 8.4% "87,491" 57.68 -0.518 ? 950509 950719 71 18.3 446 1 2 "F118/117, perfused by Dan at JAX. Agoui coat." none Jan-16-96 19 "Box 58, Slot E-1" 4 "12,874 x" 190 x RW RW and RW "3,170 mm^2" "5,263 mm^2" 31.8 µm^2 0.80% 2755 22 403 18.3 ±7.05 ±1.54 2.9403E-03 5.8265E-08 6 11 19 8 19 14 18 18 23 25 17 14 16 28 28 22 10 24 27 6 26 24 BXH6 F 1 2 071995F-2 "49,213" "±4,309" "49,213" "±4,309" 8.8% "73,003" 67.41 -0.963 ? 950509 950719 71 19.2 461 1 2 "F118/117, perfused by Dan at JAX. Agoui coat." none Jan-16-96 2 "Box 58, Slot F-2" 4 "12,970 x" 190 x RW RW and RW "2,648 mm^2" "5,263 mm^2" 31.3 µm^2 0.94% 2333 22 464 21.1 ±8.46 ±1.85 2.7769E-03 5.6426E-08 15 19 21 25 23 33 5 21 8 28 16 24 31 32 10 31 15 14 35 19 25 14 BXH6 F 1 2 071995G-2 "55,689" "±2,318" "55,689" "±2,318" 4.2% "71,391" 78.00 1.335 ? 950509 950719 71 19.0 460 1 2 "F118/117, perfused by Dan at JAX. Agoui coat." none Jan-18-96 3 "Box 58, Slot G-1" 2 "12,969 x" 190 x RW RW and RW "2,579 mm^2" "5,263 mm^2" 31.3 µm^2 0.96% 2281 22 537 24.4 ±4.66 ±1.02 1.0856E-02 1.9494E-07 28 20 27 27 21 13 18 23 24 26 19 22 20 28 32 24 29 30 29 29 23 25 BXH7 F 1 2 072595A-2 "60,779" #DIV/0! #DIV/0! 1 "60,779" "±3,737" "60,779" "±3,737" 6.1% "59,802" 101.63 950617 950725 38 17.0 448 1 1 "F127, perfused by Dan at JAX." none Jan-24-96 1 "Box 58, Slot P-1" 4 "13,098 x" 185 x RW RW and RW "2,057 mm^2" "5,263 mm^2" 30.7 µm^2 1.13% 1949 22 686 31.2 ±8.79 ±1.92 4.5587E-03 7.5004E-08 38 30 54 28 33 15 34 21 37 20 27 39 33 25 29 29 35 46 20 31 34 28 BXH7 poor fix F 1 2 072595B-2 "51,867" "±4,293" 8.3% "66,716" 77.74 950617 950725 38 17.2 448 1 1 "F127, perfused by Dan at JAX." 2 necrotic fibers Jan-24-96 3 "Box 58, Slot Q-2" 2 "12,960 x" 235 x RW RW and RW "3,689 mm^2" "5,263 mm^2" 31.3 µm^2 1.03% 2129 22 536 24.4 ±9.24 ±2.02 #DIV/0! #DIV/0! 30 23 18 24 33 16 24 28 27 23 23 17 22 19 14 39 5 21 36 49 24 21 BXH8 F 1 2 072495F-2 "64,631" #DIV/0! #DIV/0! 1 "64,631" "±4,486" "64,631" "±4,486" 6.9% "59,574" 108.49 950627 950724 27 13.0 396 1 1 "F108, perfused by Dan at JAX. Agouti coat." Pupillary light reflex was normal. none Jan-24-96 3 "Box 58, Slot N-1" 4 "13,014 x" 236 x RW RW and RW "3,325 mm^2" "5,263 mm^2" 31.1 µm^2 1.10% 1917 21 708 33.7 ±10.46 ±2.34 3.3724E-03 5.2179E-08 34 31 35 38 46 30 52 43 9 45 34 26 26 35+6 40 36 13 30 46 33 36 25 BXH8 poor fix M 1 2 072495G-2 "46,564" "±3,592" 7.7% "60,668" 76.75 950627 950724 27 11.0 395 0 1 "F108, perfused by Dan at JAX. Agouti coat." "This animal had a poor pupillary light reflex (4, 4)./" Jan-24-96 5 "Box 58, Slot O-2" 1 "13,011 x" 239 x RW RW and RW "3,463 mm^2" "5,263 mm^2" 31.1 µm^2 1.13% 1951 22 525 23.9 ±8.44 ±1.84 #DIV/0! #DIV/0! 21 24 29 4 17 28 31 21 19 26 26 9 26 26 38 23 32 24 8 29 33 31 BXH9 F 1 2 071995A-2 "56,967" "±2,238" "±3,876" 4 "56,830" "±3,225" "56,830" "±3,225" 5.7% "74,715" 76.06 -0.035 ? 950508 950719 72 25.0 480 1 1 "F122/121, perfused by Dan at JAX. Agouti coat." none Jan-12-96 13 "Box 57, Slot O-2" 4 "12,993 x" 190 x RW RW and RW "2,691 mm^2" "5,263 mm^2" 31.2 µm^2 0.88% 2396 21 498 23.7 ±6.02 ±1.35 5.7383E-03 1.0097E-07 27 30 20 12 22 23 18 16 18 33 22 36 25 18 26 30 22 32 23 22 23 BXH9 F 1 2 071995B-2 "62,366" "±4,565" "62,366" "±4,565" 7.3% "75,554" 82.54 1.393 ? 950508 950719 72 23.7 481 1 1 "F122/121, perfused by Dan at JAX. Agouti coat." none Jan-15-96 15 "Box 58, Slot B-2" 4 "13,015 x" 189 x RCS RW and RW "2,706 mm^2" "5,263 mm^2" 31.1 µm^2 0.82% 2431 20 513 25.7 ±8.18 ±1.88 3.1502E-03 5.0511E-08 22 27 28 34 38 30 30 9 10 37 27 28 17 20 20 30 36 29 19 22 BXH9 F 1 2 071995C-2 "53,323" "±4,485" "53,323" "±4,485" 8.4% "84,139" 63.38 -0.940 ? 950508 950719 72 22.3 469 1 1 "F122/121, perfused by Dan at JAX. Agouti coat." none Jan-16-96 17 "Box 58, Slot C-1" 4 "13,018 x" 190 x RW RW and RW "3,031 mm^2" "5,263 mm^2" 31.1 µm^2 0.81% 2709 22 433 19.7 ±7.59 ±1.66 2.7766E-03 5.2070E-08 26 19 10 23 15 23 24 26 33 19 16 25 27 31 16 12 6 23 24 19 10 6 BXH9 F 1 2 071995D-2 "55,348" "±2,785" "55,348" "±2,785" 5.0% "82,671" 66.95 -0.418 ? 950508 950719 72 23.5 477 1 1 "F122/121, perfused by Dan at JAX. Agouti coat." none Jan-16-96 19 "Box 58, Slot D-2" 4 "13,024 x" 191 x RW RW and RW "3,012 mm^2" "5,263 mm^2" 31.0 µm^2 0.83% 2664 22 457 20.8 ±4.79 ±1.05 7.4749E-03 1.3505E-07 14 19 29 20 27 21 24 26 18 19 13 20 27 23 22 20 24 21 10 15 24 21 BXH10 M 1 2 072495D-2 "64,931" #DIV/0! #DIV/0! 1 "64,931" "±4,327" "64,931" "±4,327" 6.7% "65,471" 99.18 2.055 ? 950619 950724 35 18.0 462 0 1 "F91, F19BC4, perfused by Dan at JAX. Black coat." none Jan-16-96 19 "Box 58, Slot L-1" 4 "13,040 x" 190 x RW RW and RW "2,358 mm^2" "5,263 mm^2" 31.0 µm^2 1.09% 2115 23 706 30.7 ±9.59 ±2.05 3.6262E-03 5.5847E-08 30 16 19 20 30 38 29 30 39 24 36 26 11 41 39 48 37 43 26 28 30 22 44 BXH10 poor fix M 072495E-2 950619 950724 35 NA 435 1 "F91, F19BC4, perfused by Dan at JAX. Black coat." "This animal had an aberrant pupillary light reflex (4,4 according to Dan). Poor fixation." Jan-16-96 1 "Box 58, Slot M-4" 1 "12,758 x" 243 x RW RW and RW "3,581 mm^2" "5,263 mm^2" 32.3 µm^2 #DIV/0! 0 0 0 #DIV/0! #DIV/0! #DIV/0! #DIV/0! #DIV/0! BXH11 F 1 2 071895E-2 "51,312" 1 "51,312" "±2,946" "51,312" "±2,946" 5.7% "58,767" 87.32 ? 950525 950718 54 19.7 422 1 1 F99 perfused by Dan at JAX. Black coat. "Good tissue, but poor staining of thin section." none Jan-12-96 14 "Box 57, Slot D-2" 2 "12,987 x" 244 x RW RW and RW "3,489 mm^2" "5,263 mm^2" 31.2 µm^2 1.06% 1883 20 545 27.3 ±6.82 ±1.56 6.2225E-03 1.2127E-07 44 34 28 20 21 23 25 18 23 24 30 25 34 24 18 40 30 29 28 27 BXH12 F 1 2 071895F-2 "69,674" ±791 "±1,369" 4 "69,436" "±5,373" "69,436" "±5,373" 7.7% "82,050" 84.63 -0.174 ? 950518 950718 61 18.2 429 1 1 "F45/44, perfused by Dan and JAX. Agouti coat." none Jan-9-96 15 "Box 57, Slot E-3" 4 "13,123 x" 191 x RW RW and RW "2,994 mm^2" "5,263 mm^2" 30.6 µm^2 0.82% 2685 22 569 25.9 ±9.17 ±2.00 2.5194E-03 3.6284E-08 38 34 15 41 29 25 17 13 28 39 40 26 19 15 19 30 19 17 21 38 27 19 BXH12 F 1 2 071895G-2 "71,498" "±6,213" "71,498" "±6,213" 8.7% "101,220" 70.64 1.332 ? 950518 950718 61 18.5 437 1 1 "F45/44, perfused by Dan and JAX. Agouti coat." none Jan-9-96 17 "Box 57, Slot F-2" 4 "13,104 x" 191 x RW RW and RW "3,698 mm^2" "5,263 mm^2" 30.7 µm^2 0.70% 3302 23 498 21.7 ±8.82 ±1.88 1.9366E-03 2.7086E-08 34 17 14 12 21 10 18 18 18 20 16 39 17 16 22 24 38 36 11 29 29 27 12 BXH12 F 1 2 071895H-2 "68,179" "±2,948" "68,179" "±2,948" 4.3% "81,359" 83.80 -1.092 ? 950518 950718 61 17.0 see note 1 1 "F45/44, perfused by Dan and JAX. Agouti coat." Check brain weight. Initial weight by Richard was a whopping 585 mg. none Jan-9-96 20 "Box 57, Slot G-1" 4 "13,133 x" 190 x RW RW and RW "2,947 mm^2" "5,263 mm^2" 30.5 µm^2 0.79% 2666 21 537 25.6 ±4.95 ±1.11 8.2351E-03 1.2079E-07 22 32 20 30 22 27 32 28 29 15 32 26 33 21 25 24 22 27 21 29 20 BXH12 M 1 2 071895I-2 "69,583" "±5,176" "69,583" "±5,176" 7.4% "87,884" 79.18 -0.066 ? 950529 950718 50 21.5 430 0 2 "F44, perfused by Dan at JAX." none Jan-17-96 1 "Box 57, Slot I-1" 4 "13,165 x" 190 x RW RW and RW "3,188 mm^2" "5,263 mm^2" 30.4 µm^2 0.76% 2894 22 529 24.0 ±8.20 ±1.79 2.7208E-03 3.9101E-08 24 6 23 24 25 29 20 38 26 33 28 22 23 43 27 20 32 20 20 20 14 12 BXH14 F 1 2 071895M-2 "66,023" "±3,297" "±7,372" 6 "72,980" "±3,273" "72,980" "±3,273" 4.5% "92,776" 78.66 0.944 ? 950526 950718 53 21.0 424 1 1 "F112, perfused by Dan at JAX. Agouti coat." This strain has an aberrant pupillary light reflex. none Jan-12-96 8 "Box 57, Slot M-2" 4 "13,052 x" 189 x RW RW and RW "3,323 mm^2" "5,263 mm^2" 30.9 µm^2 0.77% 3003 23 559 24.3 ±5.11 ±1.09 7.1244E-03 9.7621E-08 21 22 34 20 21 19 31 18 20 23 23 30 26 32 27 24 28 13 30 28 22 25 22 BXH14 F 1 2 071895N-2 "65,614" "±3,728" "65,614" "±3,728" 5.7% "77,336" 84.84 -0.056 ? 950526 950718 53 21.8 417 1 1 "F112, perfused by Dan at JAX. Agouti coat." This strain has an aberrant pupillary light reflex. none Jan-12-96 11 "Box 57, Slot N-1" 4 "13,002 x" 190 x RW RW and RW "2,778 mm^2" "5,263 mm^2" 31.1 µm^2 0.97% 2484 24 634 26.4 ±7.20 ±1.50 4.9259E-03 7.5074E-08 32 25 29 24 27 22 3 27 28 35 21 26 25 24 30 37 25 18 24 28 32 35 37 20 BXH14 F 1 2 071995H-2 "72,219" "±4,055" "72,219" "±4,055" 5.6% "76,827" 94.00 0.840 ? 950530 950719 50 19.0 438 1 2 "F112, perfused by Dan at JAX. Agouti coat." This strain has an aberrant pupillary light reflex. Gorgeous fixation. none Jan-18-96 5 "Box 58, Slot H-2" 5 "13,011 x" 189 x RW RW and RW "2,756 mm^2" "5,263 mm^2" 31.1 µm^2 0.89% 2471 22 643 29.2 ±7.52 ±1.64 4.6005E-03 6.3702E-08 32 19 26 32 17 40 34 44 32 28 30 23 27 31 30 38 35 39 24 25 21 16 BXH14 F 1 2 071995I-2 "60,788" "±4,285" "60,788" "±4,285" 7.0% "63,406" 95.87 -0.710 ? 950530 950719 50 17.6 417 1 2 "F112, perfused by Dan at JAX. Agouti coat." This individual has a white belly spot. This strain has an aberrant pupillary light reflex. none Jan-18-96 7 "Box 58, Slot I-1" 4 "12,969 x" 191 x RW RW and RW "2,320 mm^2" "5,263 mm^2" 31.3 µm^2 1.09% 2026 22 660 30.0 ±9.69 ±2.11 3.4686E-03 5.7061E-08 14 29 33 35 21 12 26 46 34 36 30 22 31 25 26 32 49 44 19 26 42 28 BXH14 F 1 2 071995J-2 "54,231" "±2,314" "54,231" "±2,314" 4.3% "65,009" 83.42 -1.599 ? 950530 950719 50 17.8 416 1 2 "F112, perfused by Dan at JAX. Agouti coat." This strain has an aberrant pupillary light reflex. none Jan-26-96 10 "Box 58, Slot J-2" 4 "12,945 x" 193 x RW RW and RW "2,412 mm^2" "5,263 mm^2" 31.4 µm^2 0.97% 2070 20 524 26.2 ±4.87 ±1.12 1.0660E-02 1.9657E-07 25 28 31 16 15 28 26 25 24 28 27 30 32 32 29 26 32 19 24 27 BXH14 F 1 2 071995K-2 "70,307" "±4,834" "70,307" "±4,834" 6.9% "82,565" 85.15 0.581 ? 950530 950719 50 18.5 415 1 2 "F112, perfused by Dan at JAX. Agouti coat." This strain has an aberrant pupillary light reflex. none Jan-17-96 12 "Box 58, Slot K-2" 4 "13,070 x" 246 x RW RW and RW "4,984 mm^2" "5,263 mm^2" 30.8 µm^2 0.78% 2680 21 551 26.2 ±8.07 ±1.80 3.1591E-03 4.4934E-08 17 12 26 25 20 29 15 26 25 24 29 36 43 31 24 26 38 37 15 31 22 BXH19 F 1 2 071795G-2 "54,136" "±2,660" "±4,608" 4 "59,761" "±5,361" "59,761" "±5,361" 9.0% "63,648" 93.89 1.221 ? 950518 950707 50 20.3 458 1 1 "F116, perfused by Dan at JAX. Non-agouti, black coat." none Jan-9-96 20 "Box 56, Slot R-1" 4 "13,158 x" 244 x RW RW and RW "3,791 mm^2" "5,263 mm^2" 30.4 µm^2 1.05% 2094 22 628 28.5 ±11.73 ±2.56 2.1786E-03 3.6455E-08 53 43 31 14 29 14 21 51 20 27 39 15 28 8 19 35 26 37 26 30 37 25 BXH19 F 1 2 071895J-2 "52,758" "±2,467" "52,758" "±2,467" 4.7% "58,821" 89.69 -0.299 ? 950606 950718 42 16.8 416 1 2 "F116, perfused by Dan at JAX. Non-agouti, black coat." none Jan-11-96 2 "Box 57, Slot J-2" 4 "13,102 x" 244 x RW RW and RW "3,492 mm^2" "5,263 mm^2" 30.7 µm^2 1.15% 1918 22 605 27.5 ±5.89 ±1.29 9.0781E-03 1.7207E-07 32 32 27 24 27 28 24 22 20 38 22 33 22 33 21 28 22 19 30 30 41 30 BXH19 F 1 2 071895K-2 "55,262" "±3,759" "55,262" "±3,759" 6.8% "67,079" 82.38 0.245 ? 950606 950718 42 16.0 412 1 2 "F116, perfused by Dan at JAX. Non-agouti, black coat." none Jan-11-96 5 "Box 57, Slot K-2" 4 "13,005 x" 189 x RW RW and RW "2,403 mm^2" "5,263 mm^2" 31.1 µm^2 1.02% 2156 22 564 25.6 ±7.99 ±1.74 4.0970E-03 7.4137E-08 22 37 28 14 26 17 37 22 34 12 25 26 26 37 15 20 32 37 28 24 15 30 BXH19 F 1 2 071895L-2 "48,761" "±3,292" "48,761" "±3,292" 6.8% "74,538" 65.42 -1.166 ? 950606 950718 42 18.0 442 1 2 "F116, perfused by Dan at JAX. Non-agouti, black coat." none Jan-11-96 6 "Box 57, Slot L-1" 4 "13,059 x" 190 x RW RW and RW "2,685 mm^2" "5,263 mm^2" 30.9 µm^2 0.87% 2415 21 424 20.2 ±6.10 ±1.36 4.7243E-03 9.6887E-08 25 25 20 27 23 14 19 37 19 14 24 10 25 16 17 22 18 24 17 13 15 C3H/HeJ-c<9J>/c<9J>-rd/rd F 1 1 072893D "67,039" "±1,625" "±3,634" 6 "66,768" "±4,072" "66,768" "±4,072" 6.1% "96,969" 68.86 -0.075 JR0339 ? 510 930412 930728 107 30.0 NA 15.4 1 1 "Albinos obtained at JAX, summer 93. Carries the rd mutation." none Feb-9-94 C2 "Box 24, Slot M-2" 4 "13,038 x" 248 x TK TK and RW "5,982 mm^2" "5,442 mm^2" 32.0 µm^2 0.76% 3029 23 507 22.0 ±6.31 ±1.34 4.2088E-03 6.3036E-08 64156 69158 107.8% " 5,002 " 16 23 28 11 23 27 20 24 19 19 28 28 30 22 28 25 23 31 16 18 25 5 18 C3H/HeJ-c<9J>/c<9J>-rd/rd original F 1 1 072893E "60,818" "±2,580" "59,061" "±3,202" 5.4% "89,782" 65.78 -1.712 JR0339 ? 930412 930728 107 40.0 NA 1 1 "Albinos obtained at JAX, summer 93. Carries the rd mutation." none Feb-9-94 C4 "Box 24, Slot N-1" 3 "13,046 x" 248 x TK TK and RW "6,145 mm^2" "5,446 mm^2" 32.0 µm^2 0.75% 2806 21 442 21.0 ±5.10 ±1.14 9.5907E-03 1.5770E-07 65941 52804 80.1% " 13,137 " 18 20 21 25 22 18 20 29 23 27 15 33 17 20 17 26 14 13 23 24 17 C3H/HeJ-c<9J>/c<9J>-rd/rd replicate 0.00001 072893E "64,070" "±4,357" 6.8% "85,425" 75.00 Replicate of different section. Apr-17-95 C4 "Box 24, Slot N-1" "13,039 x" 192 x RW RW and RW "3,645 mm^2" "5,270 mm^2" 31.0 µm^2 0.87% 2756 24 558 23.3 ±7.58 ±1.58 24 17 30 25 25 18 0 22 18 23 21 28 27 40 16 34 25 17 31 28 20 21 23 25 C3H/HeJ-+/c<9J>-rd/rd F 1 1 072893G "71,259" "±4,292" "71,259" "±4,292" 6.0% "89,782" 79.37 1.161 JR0339 ? 930412 930728 107 29.0 NA 14.0 1 1 "Albinos obtained at JAX, summer 93. Carries the rd mutation." Excellent fixation none Feb-9-94 D3 "Box 24, Slot P-1" 3 "13,058 x" 248 x TK TK and RW "5,539 mm^2" "5,450 mm^2" 32.0 µm^2 0.96% 2809 27 685 25.4 ±7.79 ±1.53 4.0176E-03 5.6380E-08 73025 69614 95.3% " 3,411 " 24 23 24 16 11 19 29 more to the right 17 38 29 25 28 25 27 13 14 26 30 33 22 15 24 39 38 34 30 32 C3H/HeJ-+/c<9J>-rd/rd please reshoot 1E-08 072893G C3H/HeSnJ-+/+ M 1 1 072394E "69,472" "±4,696" "69,472" "±4,696" 6.8% "86,635" 80.19 0.670 JR0339 ? 940613 940723 40 22.0 504 13.1 0 3 HRP injection. Retinal degeneration. Blind after 6 weeks. none Oct-24-94 C1 "Box 29, Slot A-1" 3 "13,053 x" 191 x RW RW and RW "3,165 mm^2" "5,418 mm^2" 31.8 µm^2 0.88% 2724 24 612 25.5 ±8.27 ±1.72 3.2867E-03 4.7309E-08 72876 66065 90.7% " 6,811 " 14 18 36 29 28 36 13 6 19 37 30 34 32 30 28 27 22 35 21 26 29 16 19 27 C3H/HeSnJ-+/+ F 1 1 072394F "68,283" "±4,680" "68,283" "±4,680" 6.9% "84,351" 80.95 0.342 JR0339 ? 940511 940723 73 29.0 511 1 2 HRP injection. Retinal degeneration. Blind after 6 weeks. none Oct-24-94 C4 "Box 29, Slot B-2" 3 "13,068 x" 180 x RW RW and RW "2,729 mm^2" "5,418 mm^2" 31.7 µm^2 0.71% 2659 19 488 25.7 ±7.47 ±1.76 3.2905E-03 4.8190E-08 61145 74703 122.2% " 13,558 " 29 27 42 22 38 30 15 21 22 25 27 16 26 33 31 21 24 12 27 C3H/HeSnJ-+/+ F 1 1 072394G "65,634" "±7,979" "65,634" "±7,979" 12.2% "73,404" 89.41 -0.387 JR0339 ? 940511 940723 73 29.0 516 1 2 HRP injection. Section cut close to lamina cribrosa. Photographed at higher than typical magnificiation. none Oct-24-94 C6 "Box 29, Slot C-1" 3 "16,125 x" 180 x RW RW and RW and RW "2,375 mm^2" "5,418 mm^2" 20.8 µm^2 0.54% 3523 19 354 18.6 ±9.61 ±2.27 1.0881E-03 1.6578E-08 67321 64112 95.2% " 3,209 " 12 1 9 18 15 17 10 21 17 10 14 18 23 34 24 25 43 28 15 C3H/HeJ-c<9J>/c<9J>-rd/rd poor fix F 072893F "83,533" JR0339 ? 930412 930728 107 33.0 NA 15.7 1 "Albinos obtained at JAX, summer 93. Carries the rd mutation." Poor fixation. Unmyelinated fibers hard to count. Probably a low count. Some necrosis Feb-9-94 D1 "Box 24, Slot O-2" "13,048 x" 249 x TK TK and RW "5,179 mm^2" "5,460 mm^2" 32.1 µm^2 0.84% 2605 22 455 20.7 ±8.72 ±1.90 33 28 23 25 30 32 18 10 17 21 22 1 27 10 17 34 27 19 10 24 16 11 C3H-HeSnJ-chp/chp M 5 071095F-2 950223 950710 137 NA 415 1 "#1140, cerebellar hypoplasia homozygote" C3H-HeSnJ-chp/+ M 5 071095G-2 950223 950710 137 NA 501 1 "#1143, cerebellar hypoplasia heterozygote" C57BL/6JAx1 original F 1 1 011795A "66,082" "±1,605" "±4,540" 9 "58,641" "±2,934" "59,788" "±4,304" 7.2% "79,650" 75.06 -1.639 JR000664 from Annex 1 ? 459 941213 950117 35 17.5 467 1 2 "JAX, room AX-1, section 38; ref #219758. Pedigree number 933627-21-2" "On diet 911A, 9% fat with slightly more calcium." none Feb-23-95 14 "Box 39, Slot D-2" 4 "13,131 x" 194 x RCS TK and RW "2,990 mm^2" "5,418 mm^2" 31.4 µm^2 0.67% 2535 17 401 23.6 ±6.79 ±1.70 7.2363E-03 1.2340E-07 57979 61393 105.9% " 3,415 " 28 26 14 9 30 27 27 28 39 19 19 25 22 23 19 26 20 C57BL/6JAx1 replicate 0.00001 011795A "57,645" "±4,011" 7.0% "79,187" 72.80 Replicate on different thin section (original section has hole) none Mar-20-95 14 "Box 39, Slot D-4" "10,889 x" 192 x TK RW and RW "2,934 mm^2" "5,418 mm^2" 45.7 µm^2 1.10% 1733 19 632 33.3 ±9.82 ±2.31 48 33 38 30 41 42 29 57 28 21 37 32 17 38 23 26 28 25 39 C57BL/6JAx1 F 1 1 011795B "67,249" "±3,497" "67,249" "±3,497" 5.2% "78,281" 85.91 0.257 JR000664 from Annex 1 ? 941213 950117 35 16.0 454 1 2 "JAX, room AX-1, section 38; ref #219758. Pedigree number 933627-21-2" "On diet 911A, 9% fat with slightly more calcium." none Feb-24-95 13 "Box 39, Slot E-1" 3 "13,142 x" 194 x TK TK and TK "2,931 mm^2" "5,418 mm^2" 31.4 µm^2 0.80% 2495 20 539 27.0 ±6.11 ±1.40 5.7890E-03 8.6084E-08 64876 69617 107.3% " 4,741 " 32 36 36 32 32 23 23 19 21 16 25 18 27 28 23 34 34 24 26 30 C57BL/6JAx1 F 1 1 011795C "67,796" "±3,682" "67,796" "±3,682" 5.4% "51,675" 131.20 0.377 JR000664 from Annex 1 ? 941213 950117 35 16.8 471 1 2 "JAX, room AX-1, section 38; ref #219758. Pedigree number 933627-21-2" "On diet 911A, 9% fat with slightly more calcium." none Feb-27-94 17 "Box 39, Slot F-2" 2 "13,207 x" 192 x TK TK and RW "1,896 mm^2" "5,418 mm^2" 31.1 µm^2 1.20% 1664 20 815 40.8 ±9.65 ±2.21 5.2652E-03 7.7663E-08 65714 69873 106.3% " 4,159 " 47 45 64 42 32 45 25 42 32 22 36 35 51 49 46 31 40 41 47 43 C57BL/6JAx1 original M 1 1 011795D "69,352" "±3,151" "72,912" "±5,453" 7.5% "111,077" 65.64 0.720 JR000664 from Annex 1 ? 941213 950117 35 19.5 474 0 2 "JAX, room AX-1, section 38; ref #219758. Pedigree number 933627-21-2" "On diet 911A, 9% fat with slightly more calcium. Please check area and make note." none Feb-27-94 20 "Box 39, Slot G-1" 3 "13,189 x" 193 x TK TK and RW "4,154 mm^2" "5,418 mm^2" 31.1 µm^2 0.76% 3566 27 552 20.4 ±7.80 ±1.53 7.2538E-03 1.0459E-07 78966 66387 84.1% " 12,580 " 20 21 37 22 18 26 34 29 17 13 18 18 22 19 22 31 18 7 21 8 27 9 15 26 11 13 30 C57BL/6JAx1 replicate 0.00001 011795D "67,568" "±3,861" 5.7% "102,374" 66.00 Replicate of a different thin section none May-5-95 20 "Box 39, Slot G-1" "10,895 x" 206 x RW RW and RW "4,361 mm^2" "5,270 mm^2" 44.4 µm^2 1.00% 2306 23 674 29.3 ±7.85 ±1.67 33 36 26 19 29 41 22 28 31 33 25 36 19 29 23 27 25 35 23 15 45 30 44 C57BL/6JAx1 M 1 1 091994A "69,497" "±3,394" "69,497" "±3,394" 4.9% "82,052" 84.70 0.752 JR000664 from Annex 1 ? 940612 940917 97 24.0 462 0 1 "JAX, room AX-1" "On diet 911A, 9% fat with slightly more calcium." none Dec-1-94 18 "Box 20, Slot S-2" 4 "13,023 x" 193 x RCS RW and RW "3,066 mm^2" "5,418 mm^2" 31.9 µm^2 0.66% 2568 17 460 27.1 ±5.29 ±1.32 6.4117E-03 9.2258E-08 72554 66776 92.0% " 5,779 " 32 38 29 23 27 24 21 22 20 28 35 29 24 34 22 28 24 C57BL/6JAx1 F 1 1 010695C "59,267" "±3,438" "59,267" "±3,438" 5.8% "101,402" 58.45 0.948 ? 940713 950106 177 23.0 436 1 5 "Breeder #2. From Jax Sept 1994. Used to generate F1 with BALB/c. Also mated to C57BL/6JAx1 (RCS case numbers 186, 187, 188). Probably from Annex 1. Get pedigree expansion number" Very large axons. This case was initially mislabeled CAST/Ei originally 013095C. Two errors. 1 necrotic Feb-21-95 5 "Box 39, Slot C-1" 4 "13,069 x" 193 x TK RW and RW "3,789 mm^2" "5,418 mm^2" 31.7 µm^2 0.75% 3196 24 445 18.5 ±5.16 ±1.08 5.2324E-03 8.8285E-08 60730 57800 95.2% " 2,930 " 19 13 16 7 17 21 23 15 12 23 18 14 23 24 18 19 15 25 22 27 26 10 19 19 C57BL/6JAx1 Get body weight F 1 1 041195A "64,245" "±4,987" "64,245" "±4,987" 7.8% "79,145" 81.17 1.903 ? 940820 950409 232 GET See notes 1 5 Breeder #1 from Jax September 1994. Used to generate F1 with BALB/c. Probably from Annex 1. Get pedigree expansion number. 390 mg brain. Age effect on brain weight. "Inadvertently photographed at 15,000X nominal mag." 2 necrotic fibers May-22-95 3 "16,112 x" 192 x RCS RW and RW "2,916 mm^2" "5,270 mm^2" 20.3 µm^2 0.59% 3899 23 379 16.5 ±6.00 ±1.28 2.7012E-03 4.2045E-08 13 11 17 21 11 10 20 24 22 13 14 21 16 9 18 25 21 13 20 2 25 11 22 C57BL/6JAx1 F 041195B 950309 950409 31 14.5 428 5 "Progeny of Breeder 1 (041195A) by 3 unanalyzed C57BL/6JAx1 males from Jax production colony received Jan 12, 1995. Other C57BL/6Js received with these males gave high counts (C57BL/6JAx1). Born in our colony." C57BL/6JAx1 F 041195C 950309 950409 31 15.5 478 5 "Progeny of Breeder 1 (041195A) by 3 unanalyzed C57BL/6JAx1 males from Jax production colony received Jan 12, 1995. Other C57BL/6Js received with these males gave high counts (C57BL/6JAx1). Born in our colony." C57BL/6JAx1 M 041195D 950309 950409 31 18.3 447 5 "Progeny of Breeder 1 (041195A) by 3 unanalyzed C57BL/6JAx1 males from Jax production colony received Jan 12, 1995. Other C57BL/6Js received with these males gave high counts (C57BL/6JAx1). Born in our colony." C57BL/6JAx1 041195E C57BL/6JAx1 M 1 1 091994B "71,770" "±4,971" "71,770" "±4,971" 6.9% "92,149" 77.88 1.253 JR000664 from Annex 1 ? 940612 940917 97 28.0 475 0 1 "JAX, room AX-1" "On diet 911A, 9% fat with slightly more calcium." none Dec-1-94 20 "Box 33, Slot A-1" 4 "12,946 x" 193 x RCS RW and RW "3,429 mm^2" "5,418 mm^2" 32.3 µm^2 0.60% 2851 17 428 25.2 ±6.98 ±1.74 3.0858E-03 4.2996E-08 59863 85162 142.3% " 25,299 " 21 28 16 23 16 35 28 26 16 36 26 21 24 32 23 38 19 C57BL/6JAx1 "right or left, Rob, check the count" M 1 1 091994C "66,923" "±2,665" "67,452" "±3,741" 5.5% "97,139" 69.44 0.185 JR000664 from Annex 1 R or L 940612 940917 97 22.0 456 0 1 "JAX, room AX-1" "On diet 911A, 9% fat with slightly more calcium." none Dec-7-94 21 "Box 34, Slot F-2" 3 "13,100 x" 193 x RW RW "3,618 mm^2" "5,418 mm^2" 31.6 µm^2 0.85% 3077 26 570 21.9 ±6.08 ±1.22 9.8032E-03 1.4649E-07 77154 57747 74.8% " 19,407 " 25 29 11 22 18 24 23 26 18 20 21 22 12 36 22 24 19 25 25 23 23 27 23 24 4 24 C57BL/6JAx1 left or right 0.00001 091994C "66,377" "±3,796" 5.7% "102,191" 64.95 JR000664 L or R 940612 940917 dup 1 "JAX, room AX-1" Dec-8-94 1 "Box 33, Slot P-1" "12,976 x" 193 x RCS RW and RW "3,803 mm^2" "5,418 mm^2" 32.2 µm^2 0.63% 3176 20 418 20.9 ±5.21 ±1.20 68916 63834 92.6% " 5,081 " 26 21 22 22 19 17 17 22 17 18 23 22 26 30 11 10 22 27 18 28 C57BL/6J F 1 1 122192E "54,320" "±1,347" "±5,216" 16 "58,072" "±3,097" "58,072" "±3,097" 5.3% "74,354" 78.10 0.719 "56,848 " JR000664 All of these 1992 C57BL/6 are from Dan's breeding colony. The foundation mice received from JAX (Dan's numbers 1759-1762 from Dan's lab book) between 12/4/89 and 2/20/90. L 444 921114 921222 38 442 14.1 1 1 "Black mouse strain. Origin from Little 1921. Black 6 substrain separated prior to 1937. Substrains 6 and 10 differe at H-9, Igh-2, and Lv loci" "All of these 1992 C57BL/6 are from Dan's breeding colony. The foundation mice received from JAX (Dan's numbers 1759-1762) between 12/4/89 and 2/20/90. Weird myelination, huge axons" Jan-12-93 D2 "Box 21, Slot PÐ1" 3 "13,379 x" 202 x DR DR and RW "3,034 mm^2" "5,208 mm^2" 29.1 µm^2 1.13% 2556 29 659 22.7 ±6.41 ±1.21 6.2689E-03 1.0795E-07 57865 58266 100.7% 402 28 23 16 12 23 26 24 32 23 8 17 32 23 34 18 24 19 21 26 17 25 14 32 22 25 29 30 20 16 C57BL/6J F 1 1 122192C "53,651" "±2,045" "±4,574" 6 "56,008" "±3,230" "56,008" "±3,230" 5.8% "57,361" "5,972" "1,148" 97.64 0.324 "49,321 " JR000664 All of these 1992 C57BL/6 are from Dan's breeding colony. The foundation mice received from JAX (Dan's numbers 1759-1762 from Dan's lab book) between 12/4/89 and 2/20/90. R 921114 921223 39 19.0 426 14.2 1 1 6-8 weeks old. 10% unmyelinated Direct determination of sample area. All of these 1992 C57BL/6 are from Dan's breeding colony. The foundation mice received from JAX (numbers 1759-1762) between 12/4/89 and 2/20/90. "3 necrotic, 0016 and 0011" Jan-9-93 C5 "Box 21, Slot OÐ2" 4 "13,322 x" 199 x DR DR and RW "2,272 mm^2" "5,208 mm^2" 29.3 µm^2 1.17% 1958 23 658 28.6 ±7.74 ±1.65 5.6125E-03 1.0021E-07 55349 62244 112.5% " 6,895 " 29 32 21 41 32 28 30 35 32 32 15 20 29 33 24 27 38 34 33 38 12 14 29 C57BL/6J original F 1 1 091092A "54,274" "±2,425" "56,612" "±4,262" 7.5% "98,219" 57.64 -0.009 "45,895 " JR000664 All of these 1992 C57BL/6 are from Dan's breeding colony. The foundation mice received from JAX (Dan's numbers 1759-1762 from Dan's lab book) between 12/4/89 and 2/20/90. ? 920503 921109 190 see notes 14.9 1 2 very thick myelin. Brain weight of 0.398 gm does not include part of cerebellum. Fixed with 4% paraformaldehyde and then postfixed overnight in 1/4 strength Karnovsky fix. All of these 1992 C57BL/6 are from Dan's breeding colony. The foundation mice received from JAX (numbers 1759-1762) between 12/4/89 and 2/20/90. "Redone by RW Sept 22, 1994, 12/4/1992" A4 "Box 20, Slot N-1" 3 "12,992 x" 192 x RW RW and RW "3,619 mm^2" "5,418 mm^2" 32.1 µm^2 0.72% 3060 22 407 18.5 ±6.38 ±1.39 9.6704E-03 1.7818E-07 46772 68023 145.4% " 21,251 " 9 18 18 24 15 20 16 18 26 22 18 19 16 37 16 21 8 23 8 21 13 21 C57BL/6J replicate 0.00001 091092A "53,156" "±2,948" 5.5% "92,835" 57.26 "45,895 " JR000664 All of these 1992 C57BL/6 are from Dan's breeding colony. The foundation mice received from JAX (Dan's numbers 1759-1762 from Dan's lab book) between 12/4/89 and 2/20/90. ? 920503 921109 190 15.6 dup 2 very thick myelin "Redone by DR Sept 27, 1994, 12/4/1992" A4 "Box 20, Slot N-1" "12,992 x" 192 x DR DR and RW "3,425 mm^2" "5,418 mm^2" 32.1 µm^2 1.00% 2892 29 533 18.4 ±5.39 ±1.02 53455 57769 108.1% " 4,314 " 21 7 21 18 13 10 22 12 21 21 13 20 19 19 18 29 23 16 18 15 30 21 20 14 20 20 8 25 19 C57BL/6J original M 1 1 091092B "47,070" "±1,945" "48,404" "±3,264" 6.7% "89,113" "1,975" 501 54.32 -1.390 "38,596 " JR000664 All of these 1992 C57BL/6 are from Dan's breeding colony. The foundation mice received from JAX (Dan's numbers 1759-1762 from Dan's lab book) between 12/4/89 and 2/20/90. R 920503 921109 190 see notes 0 2 "First set miscalibrated. The high mag calibration was out of focus and was shot after the low mag montage and calibration. It is wrong. Mag probably about 13,000. Redone by Rob. Very thick myelin, low contrast stain." 4.5% unmyelinated. May be a slight underestimate. May have failed to count a small number in the sampling micrographs. Brain weight of 0.358 does not include cerebellum. none "Redone by RW Sept 22, 1994. 12/23/1992" A6 "Box 21, Slot J-2" 3 "13,053 x" 192 x RW RW and RW "3,302 mm^2" "5,418 mm^2" 31.8 µm^2 0.79% 2802 22 380 17.3 ±5.34 ±1.16 1.3036E-02 2.7694E-07 44880 52020 115.9% " 7,140 " 17 16 20 25 16 15 21 6 20 10 25 22 13 17 13 13 27 15 13 24 19 13 C57BL/6J replicate 0.00001 091092B "46,337" "±2,422" 5.2% "89,862" "1,975" 501 51.56 "38,596 " JR000664 All of these 1992 C57BL/6 are from Dan's breeding colony. The foundation mice received from JAX (Dan's numbers 1759-1762 from Dan's lab book) between 12/4/89 and 2/20/90. R 920503 921109 190 dup 2 This is a 2nd repeat by Denny. Fixed with 4% paraformaldehyde and then postfixed overnight in 1/4 strength Karnovsky fix. Sep-27-94 A6 "Box 21, Slot J-4" "13,085 x" 193 x DR DR and RW "3,363 mm^2" "5,418 mm^2" 31.6 µm^2 0.77% 2840 22 359 16.3 ±3.91 ±0.85 48593 50936 104.8% " 2,343 " 11 16 21 18 18 17 6 18 16 16 22 17 11 19 19 18 19 13 21 18 11 14 C57BL/6J M 1 1 011994G "57,450" "±3,599" "57,450" "±3,599" 6.3% "95,543" 60.13 0.600 ? NA 941109 100 25.0 463 0 4 3 M (7-8 weeks upon arrival) and 8 F (4-5 weeks upon arrival) received from Harlan shipped 12/93. This is one of these Harlan animals. 1 necrotic in negative 024 Apr-4-94 6 "Box 26, Slot C-1" 3 "12,924 x" 195 x TK TK and RW "3,627 mm^2" "5,432 mm^2" 32.5 µm^2 0.92% 2938 27 528 19.6 ±6.25 ±1.23 4.6053E-03 8.0162E-08 52653 61902 117.6% " 9,249 " 6 12 13 10 18 13 15 23 24 16 19 25 28 23 18 21 26 23 23 23 25 11 24 16 33 17 23 C57BL/6J F 1 1 011994H "49,028" "±4,163" "49,028" "±4,163" 8.5% "74,663" 65.67 -1.015 ? NA 941109 100 22.8 446 1 4 3 M (7-8 weeks upon arrival) and 8 F (4-5 weeks upon arrival) received from Harlan shipped 12/93. This is one of these Harlan animals. No necrosis Apr-4-94 8 "Box 26, Slot D-2" 3 "12,931 x" 195 x TK TK and RW "2,833 mm^2" "5,441 mm^2" 32.5 µm^2 0.83% 2294 19 406 21.4 ±7.70 ±1.81 2.9867E-03 6.0918E-08 50731 47493 93.6% " 3,238 " 17 14 5 29 24 26 24 19 34 22 24 24 21 28 26 22 28 15 4 C57BL/6J M 062292B 5569 All of these 1992 C57BL/6 are from Dan's breeding colony. The foundation mice received from JAX (Dan's numbers 1759-1762 from Dan's lab book) between 12/4/89 and 2/20/90. R 920503 920622 50 25.0 NA 2 A wild type from the naked and splotch colony. N36. Has a splotch heterozygote littermate. All of these 1992 C57BL/6 are from Dan's breeding colony. The foundation mice received from JAX (JAX numbers 1759-1762) between 12/4/89 and 2/20/90. B1 "Box 16, Slot" C57BL/6JAx10 F 1 1 070695A "56,026" "±2,928" "±6,548" 6 "47,803" "±4,863" "47,803" "±4,863" 10.2% "74,562" 64.11 -1.256 ? 950706 40 17.5 479 1 5 From JAX colony Annex 10 These mice were shipped my mistake as C57BLKS/J. none Aug-14-95 3 "Box 50, Slot O-2" 3 "12,979 x" 192 x RCS RW and RW "2,763 mm^2" "5,270 mm^2" 31.3 µm^2 0.76% 2384 18 361 20.1 ±8.41 ±2.04 2.1404E-03 4.4775E-08 11 4 16 27 31 24 6 17 20 36 21 17 16 22 26 16 31 20 C57BL/6JAx10 please redo F 1 1 070695B "65,653" "±5,464" "65,653" "±5,464" 8.3% "82,127" 79.94 1.470 ? 950706 40 18.0 482 1 5 From JAX colony Annex 10 These mice were shipped my mistake as C57BLKS/J. none Aug-28-95 4 "Box 48, Slot P-1" 3 "13,121 x" 191 x RCS RW and RW "3,005 mm^2" "5,418 mm^2" 31.5 µm^2 0.73% 2610 19 478 25.2 ±8.88 ±2.09 2.3210E-03 3.5352E-08 41 32 29 25 15 29 28 40 12 27 21 32 6 25 26 19 21 18 32 C57BL/6JAx10 F 1 1 070695C "51,113" "±3,488" "51,113" "±3,488" 6.8% "77,594" 65.87 -0.750 ? 950706 40 18.3 483 1 5 From JAX colony Annex 10 These mice were shipped my mistake as C57BLKS/J. none Aug-14-95 6 "Box 50, Slot Q-2" 3 "12,718 x" 194 x RCS RW and RW "2,908 mm^2" "5,270 mm^2" 32.6 µm^2 1.09% 2382 26 558 21.5 ±7.32 ±1.46 4.3697E-03 8.5492E-08 13 9 10 23 15 26 29 32 21 21 17 29 22 27 18 28 31 6 20 27 32 15 20 19 29 19 C57BL/6JAx10 F 1 1 070695D "53,551" "±4,167" "53,551" "±4,167" 7.8% "63,172" 84.77 -0.378 ? 950706 40 17.8 496 1 5 From JAX colony Annex 10 These mice were shipped my mistake as C57BLKS/J. none Aug-18-95 8 "Box 50, Slot R-3" 3 "12,999 x" 250 x RCS RCS and RW "3,940 mm^2" "5,270 mm^2" 31.2 µm^2 1.23% 2025 25 661 26.4 ±10.08 ±2.06 3.2125E-03 5.9991E-08 31 10 46 25 36 32 19 28 20 10 19 14 30 13 24 43 30 23 33 35 24 19 26 46 25 C57BL/6JAx10 F 1 1 070695E "60,738" "±3,574" "60,738" "±3,574" 5.9% "88,111" 68.93 0.720 ? 950706 40 18.3 482 1 5 From JAX colony Annex 10 These mice were shipped my mistake as C57BLKS/J. none Aug-18-95 10 "Box 50, Slot S-2" 3 "13,120 x" 194 x RCS RCS and RW "3,329 mm^2" "5,270 mm^2" 30.6 µm^2 0.66% 2878 19 401 21.1 ±5.27 ±1.24 5.0183E-03 8.2622E-08 30 22 26 21 19 24 22 20 28 18 23 14 14 12 31 20 21 21 15 C57BL/6JAx10 F 1 1 070695F "57,297" "±5,589" "57,297" "±5,589" 9.8% "85,789" 66.79 0.194 ? 950706 40 18.5 484 1 5 From JAX colony Annex 10 These mice were shipped my mistake as C57BLKS/J. none Aug-24-95 12 "Box 48, Slot T-1" 3 "13,118 x" 193 x RCS RW and RW "3,201 mm^2" "5,270 mm^2" 30.6 µm^2 0.79% 2801 22 450 20.5 ±9.14 ±2.00 1.9218E-03 3.3541E-08 9 31 39 11 13 19 7 24 28 25 41 16 26 15 25 17 13 24 22 13 11 21 C57BL/6JAx10 poor fix F 070695G 950706 40 18.6 506 5 From JAX colony Annex 10 Fixation is way too bad to count unmyelinated fibers. These mice were shipped my mistake as C57BLKS/J. Aug-28-95 14 "Box 52, Slot J-2" 1 "13,116 x" 193 x RCS RW and RW C57BL/6JAx10 F 070695H 950706 40 18.6 488 5 From JAX colony Annex 10 These mice were shipped my mistake as C57BLKS/J. C57BL/6J -c<2J>/c<2J> please reshoot low of nerve at 200 M 1 1 112292F "52,765" "±2,567" "±4,446" 4 "59,022" "±3,755" "59,022" "±3,755" 6.4% "75,035" 78.66 0.901 "58,699 " JR0058 R 434 920916 921122 67 26.0 467 14.9 0 1 Beautiful fix A few unmyelimated Jan-8-93 D5 "Box 21, Slot B-4" 4 "13,331 x" 254 x DR DR and RW "4,841 mm^2" "5,324 mm^2" 30.0 µm^2 1.28% 2505 32 754 23.6 ±8.35 ±1.50 4.3203E-03 7.3199E-08 57943 60136 103.8% " 2,192 " 16 27 25 19 21 28 28 23 19 27 26 27 25 13 16 14 22 21 29 38 18 27 29 12 43 43 13 12 24 10 30 29 C57BL/6J -c<2J>/c<2J> M 1 1 112292G "52,848" "±3,319" "52,848" "±3,319" 6.3% "55,025" 96.04 -0.282 "56,029 " JR0058 L 920916 921122 67 16.0 425 14.4 0 1 "Data initially screwed up. High mag shots taken at 12,000 nomimal. No calibration for this mag. Cannot verify that negatives taken from the thin section for which nerve area was determined. Have Denny reshoot. Now taken care of by Rob." Rob's estimate of nerve area is 28752 µm^2. Mismatch between high mag samples and the montage. Is this the same nerve? Sep-28-93 B5 "Box 21, Slot A-1" 3 "13,036 x" 244 x DR DR and RW recounted by TK "3,273 mm^2" "5,392 mm^2" 31.7 µm^2 1.10% 1734 19 579 30.5 ±8.12 ±1.91 5.0640E-03 9.5822E-08 50471 55500 110.0% " 5,030 " 42 32 20 34 24 28 28 23 35 31 23 33 34 34 38 35 8 38 39 C57BL/6J -c<2J>/c<2J> F 1 1 062892G "49,422" "±3,358" "49,422" "±3,358" 6.8% "55,149" 89.62 -0.939 "53,392 " JR0058 JAX1079 R 920430 920628 59 18.0 409 14.8 1 2 "Denny used the area of ipsi retina to estimate labeled cells in contra. Formvar is bad, count is fine" Dec-21-92 A5 "Box 17, Slot C-1" 3 "13,312 x" 254 x DR and RW RW and DR "3,556 mm^2" "5,324 mm^2" 30.0 µm^2 1.42% 1836 26 700 26.9 ±9.15 ±1.83 4.5573E-03 9.2212E-08 53364 54598 102.3% " 1,234 " 47 27 24 29 31 20 9 11 43 43 26 33 23 28 29 18 29 39 28 32 18 20 25 26 22 20 C57BL/6J -c<2J>/c<2J> F 1 1 061892D "49,770" "±3,589" "49,770" "±3,589" 7.2% "47,673" 104.40 -0.872 JR0058 JAX1078 R 920430 920618 49 15.0 NA 1 2 Formvar not great. Count is accurate. F48 + F16. Brain was sectioned. 15K nominal mag 1 or 2 in series Sep-21-93 A4 "Box 17, Slot E-1" 2 "14,067 x" 251 x DR and RW DR and RW "2,999 mm^2" "5,324 mm^2" 26.9 µm^2 1.30% 1772 23 646 28.1 ±9.50 ±2.03 4.0388E-03 8.1151E-08 52354 47400 90.5% " 4,953 " 46 46 30 39 30 24 37 31 34 36 19 10 14 17 20 25 21 35 24 28 25 34 21 C57BL/6J -c<2J>/c<2J> M 072795D-2 950614 950727 43 21.2 461 3 "From Room MP-22, Section 14. Perfused by Dan at JAX." C57BL/6J -c<2J>/c<2J> M 072795E-2 950614 950727 43 22.7 481 3 "From Room MP-22, Section 14. Perfused by Dan at JAX." C57BL/6J -c<2J>/c<2J> M 072795F-2 950614 950727 43 22.0 466 3 "From Room MP-22, Section 14. Perfused by Dan at JAX." C57BL/6J -c<2J>/c<2J> M 072795G-2 950614 950727 43 19.3 449 3 "From Room MP-22, Section 14. Perfused by Dan at JAX." C57BL/6J MGL 375 M 072595I-2 "64,694" "±3,763" "64,694" "±3,763" 5.8% "82,921" 78.02 1.989 950615 950725 40 22.0 459 0 7 "C57BL/6J from the JAX MMGR colony, Room MGL 375, Section 4, perfused by Dan at JAX. (250x251)-2" none Sep-19-95 18 "Box 54, Slot E-1" 4 "13,161 x" 192 x RCS RCS and RW "3,070 mm^2" "5,392 mm^2" 31.1 µm^2 0.79% 2664 21 510 24.3 ±6.32 ±1.41 4.7965E-03 7.4142E-08 23 33 25 21 24 17 20 26 29 27 16 27 16 28 25 15 37 24 20 37 20 16+1 C57BL/6J MGL 375 M 072595J-2 950615 950725 40 23.2 485 7 "C57BL/6J from the JAX MMGR colony, Room MGL 375, Section 4, perfused by Dan at JAX. (250x251)-2" C57BL/6J MGL 375 M 072595K-2 950615 950725 40 22.5 450 7 "C57BL/6J from the JAX MMGR colony, Room MGL 375, Section 4, perfused by Dan at JAX. (250x251)-2" C57BL/6J MGL 371 F 072595L-2 950525 950725 61 20.2 490 8 "F182+12. C57BL/6J from the JAX MMSC colony, Room MGL 371, perfused by Dan at JAX. JAX # 6822" C57BL/6J MGL 371 F 072595M-2 950525 950725 61 21.2 485 8 "F182+12. C57BL/6J from the JAX MMSC colony, Room MGL 371, perfused by Dan at JAX. JAX # 6823" C57BL/6J MGL 371 F 072595N-2 950525 950725 61 NA 491 8 "F182+12. C57BL/6J from the JAX MMSC colony, Room MGL 371, perfused by Dan at JAX. JAX #6824" C57BL/6J RDFS F 091895G 950404 950918 167 27.3 509 10 Get information on RDFS colony C57BL/6J RDFS M 091895H 950404 950918 167 29.0 506 10 Get information on RDFS colony C57BL/6J RDFS M 121995E 950101 951219 352 36.0 497 11 "JAX #64, non-agouti." C57BL/6J Ei M 072795A-2 950623 950727 34 22.7 480 9 "F129+48 from MMGR Room MGL 375, Section 4." C57BL/6J Ei M 072795B-2 950623 950727 34 21.8 469 9 "F129+48 from MMGR Room MGL 375, Section 4." C57BL/6J Ei M 072795C-2 950623 950727 34 21.9 476 9 "F129+48 from MMGR Room MGL 375, Section 4." C57BL/6J-cht/cht F 5 062692G "69,244" "±2,925" "69,244" "±2,925" 4.2% "146,236" 47.35 JR0976 ? 920523 920626 34 18.0 438 1 "chocholate homozygote, cht/cht, pigmented" Check the size of the nerve. Calibration looks ok but montage donw at 150X. "less than 10 necrotic axons in series, see negatives 12,25,36,41" Mar-5-93 C4 "Box 20A, Slot G-3" "10,756 x" 143 x DR RW "2,972 mm^2" "5,094 mm^2" 44.2 µm^2 1.42% 3311 47 983 20.9 ±5.99 ±0.88 8.2692E-03 1.1942E-07 71973 66629 92.6% " 5,344 " 12 21 31 23 21 26 23 21 17 17 11 32 16 29 23 18 15 32 18 18 17 12 14 7 16 24 16 18 22 23 11 22 27 22 20 24 20 19 23 19 21 18 30 27 32 28 27 C57BL/6J-sea/sea F 5 062892D "68,393" "±3,436" "69,372" "±3,436" 5.0% "72,744" 95.36 JR0108 JAX6105 R 920418 920629 72 21.2 456 1 "Sepia mutant, sea/sea. F93. HRP injection backfilled only a part of the contralateral retina" many unmyelinated(25%) No degenerating axons at all Feb-4-93 D6 "Box ?, Slot RÐ1" "13,079 x" 194 x DR DR and RW "2,738 mm^2" "5,239 mm^2" 30.6 µm^2 1.22% 2375 29 847 29.2 ±7.65 ±1.45 5.9997E-03 8.7724E-08 64130 74264 115.8% " 10,134 " 28 31 40 25 33 25 15 27 24 30 27 31 25 26 22 32 45 31 36 8 32 22 33 30 38 37 41 23 30 C57BL/6J-sea/sea 062892D "67,415" "±3,708" 5.5% "70,092" "12,864" "1,805" 96.18 JR0108 JAX6105 L 920629 dup "Sepia mutant, sea/sea. F93. HRP injection backfilled only a part of the contralateral retina" many unmyelinated(25%) Feb-10-93 A1 "Box ?, Ask Denny, Slot SÐ2" "12,960 x" 194 x DR DR and DR "2,638 mm^2" "5,239 mm^2" 31.2 µm^2 1.16% 2247 26 780 30.0 ±8.25 ±1.65 68797 66032 96.0% " 2,766 " 22 31 38 24 32 22 41 28 48 37 27 38 9 28 31 34 29 24 32 30 26 27 16 32 31 43 C57BLKS/J M 1 1 052793H "64,238" "±2,572" "±6,301" 7 "67,309" "±4,715" "67,309" "±4,715" 7.0% "93,486" 72.00 0.487 JR000662 ? 445 930420 930527 37 18.0 444 0 1 "Animal from JAX Park, not from Bst colony" none Nov-18-93 A4 "Box 23, Slot O-2" 4 "12,911 x" 247 x TK TK and RW "5,696 mm^2" "5,423 mm^2" 32.5 µm^2 0.90% 2874 26 609 23.4 ±8.20 ±1.64 3.1493E-03 4.6789E-08 71865 68861 95.8% " 3,004 " to right to right to right to right to right to right to right to right to right to right to right to right to right to right to right to right to right to right to right 23 8 19 7 26 29 10 25 34 38 24 38 26 17 32 26 30 15 12 22 26 27 24 23 25 23 C57BLKS/J F 1 1 052793I "69,608" "±3,635" 3 "61,951" "±3,793" "61,951" "±3,793" 6.1% "86,279" 71.80 -0.363 JR000662 ? 930420 930527 37 16.0 440 1 1 "Animal from JAX Park, not from Bst colony" none Nov-7-93 A6 "Box 23, Slot P-1" 4 "12,975 x" 247 x TK TK and RW "5,244 mm^2" "5,439 mm^2" 32.3 µm^2 0.94% 2670 25 580 23.2 ±6.96 ±1.42 4.4861E-03 7.2415E-08 72834 60200 82.7% " 12,633 " 12 17 12 31 13 31 19 26 26 28 22 13 21 27 11 26 27 35 33 26 25 22 23 24 30 C57BLKS/J F 1 1 052793J "60,210" "±1,942" 4 "58,625" "±4,679" "58,625" "±4,679" 8.0% "85,185" 68.82 -0.891 JR000662 ? 930420 930527 37 16.0 443 1 1 "Animal from JAX Park, not from Bst colony" none Nov-17-93 B4 "Box 23, Slot Q-2" 4 "13,142 x" 242 x TK TK and RW "4,997 mm^2" "5,401 mm^2" 31.3 µm^2 0.84% 2724 23 495 21.5 ±8.06 ±1.72 2.8001E-03 4.7762E-08 65528 63308 96.6% " 2,221 " to right to right to right to right to right to right to right to right to right to right to right to right to right to right to right to right to right to right to right to right to right to right 18 21 28 25 24 24 15 15 12 33 21 17 37 25 23 0 16 24 10 30 23 27 27 C57BLKS/J F 1 1 082094K "56,365" "±3,997" "56,365" "±3,997" 7.1% "70,142" 80.36 -1.250 JR000662 ? 940715 940820 36 17.0 425 1 2 Animal from JAX Park colony From Park colony. Marginal fixation. Count is ok. none Nov-21-94 21 "Box 20, Slot F-2" 2 "13,185 x" 192 x DR RW and RW "2,577 mm^2" "5,418 mm^2" 31.2 µm^2 0.98% 2251 22 551 25.0 ±8.14 ±1.78 3.6964E-03 6.5580E-08 55374 56799 102.6% " 1,425 " 31 31 12 32 43 34 30 21 14 36 24 22 28 18 15 20 31 17 21 31 23 17 C57BLKS/J M 1 1 021795L "75,497" "±4,590" "75,497" "±4,590" 6.1% "114,405" 65.99 1.787 JR000662 ? 941201 950217 78 22.5 463 0 3 Obtained from the Park colony. Unrelated to Bst colony. none May-23-95 7 "Box 44, Slot P-1" 4 "13,048 x" 193 x RCS DR and RW "4,275 mm^2" "5,270 mm^2" 31.0 µm^2 0.57% 3696 21 429 20.4 ±5.55 ±1.24 3.7619E-03 4.9829E-08 17 18 24 16 24 21 17 24 27 10 23 26 10 14 22 22 32 25 17 23 17 C57BLKS/J M 1 1 021795M "66,019" "±3,384" "66,019" "±3,384" 5.1% "77,237" 85.48 0.283 JR000662 ? 941201 950217 78 20.5 435 0 3 Obtained from the Park colony. Unrelated to Bst colony. none May-23-95 9 "Box 44, Slot O-2" 3 "13,058 x" 194 x RCS DR and RW "2,898 mm^2" "5,270 mm^2" 30.9 µm^2 0.96% 2499 24 634 26.4 ±6.49 ±1.35 6.0163E-03 9.1130E-08 38 35 21 25 27 24 31 26 25 7 37 35 27 27 25 19 23 24 30 28 23 27 21 29 C57BLKS/J L or R F 1 1 072893A "63,899" "±2,809" "65,158" "±3,573" 5.5% "95,937" 67.92 1.088 L or R 930526 930727 62 20.0 NA 1 4 "No pupillary response in left eye, normal in right" Jun-19-95 B3 "Box 45, Slot K-2" "12,794 x" 194 x DR DR "3,602 mm^2" "5,380 mm^2" 32.9 µm^2 1.16% 2919 34 759 22.3 ±7.03 ±1.22 8.3425E-03 1.3056E-07 25 26 16 20 12 26 17 17 14 22 21 28 13 17 18 37 24 21 25 20 28 31 24 25 40 29 26 29 18 7 16 30 20 17 C57BLKS/J R or L 072893A "61,861" "±4,547" 7.4% "85,152" R or L Other side of this case. Side unknown. 6/24/95 B4 "Box 45, Slot S-2" "12,943 x" 193 x DR DR "3,167 mm^2" "5,380 mm^2" 32.1 µm^2 0.79% 2651 21 490 23.3 ±7.67 ±1.72 25 16 28 32 30 17 14 23 21 8 16 32 38 22 25 33 16 32 24 18 20 C57BLKS/J "poor fix, not countable" M 082094J 940715 940820 36 20.0 455 2 Poor perfusion. Not countable. Keep brain weight data. C57BLKS/J "poor fix, not countable" M 082094I 940715 940820 36 18.0 450 2 Poor perfusion. Not countable. Keep brain weight data. C57BLKS/J please photograph M 040695L 941201 950406 126 25.5 468 4 Park animal. Denny's #19. Perfused by Rob and Richelle. Perfusion rated good. C57BLKS/J "poor fix, not countable" F 082094L 940715 940820 36 14.0 429 2 Poor perfusion. Not countable. Keep brain weight data. C57BLKS/J-Bst/+ R M 5 072993D "36,232" "±6,283" "±24,701" 23 "12,325" "±1,170" 9.5% "53,544" 23.02 JAX1273 R 921216 930730 226 NA affected eye of Belly spot and tail (Bst/+) mutant F35+2 N1F51. Derived from C57BL/6 in 1948 Oct-24-93 B2 "Box 23, Slot B-4" "12,998 x" 189 x DR DR "1,913 mm^2" "5,408 mm^2" 32.0 µm^2 1.14% 1673 19 140 7.4 ±2.97 ±0.70 11895 12713 106.9% 818 8 10 6 5 5 9 3 7 10 9 9 8 6 8 0 7 14 9 7 C57BLKS/J-Bst/+ "L, replicate" 072993D area of nerve "59,105" "±3,723" 6.3% "128,311" 46.06 JAX1273 L 921216 930730 226 NA dup more normal eye of mutant F35+2 N1F51. Derived from C57BL/6 in 1948 Oct-24-93 B1 "Box 23, Slot A-3" "13,164 x" 248 x DR DR "7,916 mm^2" "5,381 mm^2" 31.1 µm^2 0.56% 4132 23 329 14.3 ±4.23 ±0.90 27940 32033 114.6% " 4,093 " 15 10 19 12 20 14 19 19 12 15 14 14 8 19 18 18 15 19 13 7 14 9 6 C57BLKS/J-Bst/+ "R, poor count and calibrations-do not use" F 5 062292E "67,271" "±9,090" "±42,638" 23 "2,151" ±323 15.0% "18,293" 11.76 JR0516 JAX1203 R 920514 920623 40 17.0 NA 1 mutant eye of mutant F35+2 N1F51. Derived from C57BL/6 in 1948 Nov-25-92 C3 "Box 14, Slot P" "13,229 x" 324 x DR DR and RW "1,920 mm^2" "5,250 mm^2" 30.0 µm^2 5.90% 610 36 127 3.5 ±3.13 ±0.53 2134 2150 100.8% 16 3 0 0 5 10 5 3 3 6 4 0 7 6 1 0 6 9 7 2 5 0 1 0 5 2 4 5 0 12 6 3 1 2 4 0 0 C57BLKS/J-Bst/+ "L, replicate " 062292E only the normal nerve "65,981" "65,981" "±4,456" 6.8% "69,135" 95.44 JR0516 JAX1203 L 920514 920623 40 17.0 dup normal eye of mutant <15% unmyelinated Nov-12-92 C4 "Box 14, Slot Q" "13,152 x" 253 x DR DR "4,436 mm^2" "5,250 mm^2" 30.4 µm^2 1.36% 2278 31 898 29.0 ±10.72 ±1.96 62654 72329 115.4% " 9,675 " 36 30 35 17 31 21 32 35 35 11 38 19 22 16 25 23 38 27 21 51 37 38 15 19 6 34 41 34 23 46 42 C57BLKS/J-Bst/+ R F 5 062392A only the normal nerve "57,224" "57,224" "±4,177" 7.3% "52,178" 109.67 JR0516 R 920514 920624 41 17.0 NA 1 normal eye of mutant. Brain was sectioned. No weight data. "<10% unmyelinated, strange axon configurations" Dec-23-92 C5 "Box 21, Slot H-2" "13,478 x" 257 x DR DR and RW "3,433 mm^2" "5,250 mm^2" 28.9 µm^2 1.27% 1805 23 729 31.7 ±10.85 ±2.31 59322 57824 97.5% " 1,499 " 27 34 36 15 40 32 42 29 26 37 39 26 54 44 26 6 29 20 37 38 36 41 15 C57BLKS/J-Bst/+ L 062392A "5,079" "±1,611" 31.7% "22,796" 22.28 JR0516 L 920514 920624 41 17.0 dup "affected eye of mutant. Tail very kinked, spots along tail but no belly spot" "Few axons, web of thin glial processes. Myelin unusually thin." Apr-19-93 C6 "Box 21, Slot GÐ3" "10,923 x" 224 x DR DR "1,144 mm^2" "5,355 mm^2" 44.9 µm^2 2.17% 508 11 110 10.0 ±10.03 ±3.17 2540 7195 283.3% " 4,656 " to right 30 7 1 6 28 3 5 5 6 4 15 C57BLKS/J-Bst/+ L M 5 122993A "69,247" "69,247" "±2,923" 4.2% "80,342" 86.19 JR0516 L 931018 931229 72 25.0 460 0 "Many unmyelinated fibers, perhaps 35-40%. Brain also weighed by Denny = 0.4945 gm." Oct-6-94 9 "Box 26, Slot K-2" "12,851 x" 193 x DR DR and RW "2,988 mm^2" "5,464 mm^2" 33.1 µm^2 1.28% 2428 31 884 28.5 ±6.59 ±1.20 74914 73151 97.6% " 1,763 " 37 40 24 38 29 32 28 30 30 29 38 25 35 29 27 32 36 34 17 29 22 24 21 16 35 29 32 21 25 25 15 C57BLKS/J-Bst/+ R 122993A "29,965" "29,965" "±1,494" 5.0% "47,378" 63.25 "Jax 1506, from 1423 x 1421" Oct-6-94 10 "Box 26, Slot L-1" "13,026 x" 195 x DR DR "1,793 mm^2" "5,380 mm^2" 31.7 µm^2 1.20% 1494 18 361 20.1 ±4.12 ±1.00 24 20 25 19 19 19 22 24 26 19 22 17 12 19 17 27 16 14 C57BLKS/J-Bst/+ R F 5 122993F "18,938" "18,938" "±1,220" 6.4% "45,079" 42.01 R 930408 931229 265 28.0 "Jax #1299 used in cross to Cast/Ei, no pupil response in R eye, normal in Left" Jun-19-95 16 "Box 45, Slot Q-2" "12,897 x" 190 x DR DR "1,626 mm^2" "5,380 mm^2" 32.3 µm^2 1.22% 1394 17 231 13.6 ±3.50 ±0.88 14 20 9 8 11 17 15 8 13 14 12 12 15 18 12 18 15 C57BLKS/J-Bst/+ L 122993F "61,426" "61,426" "±4,414" 7.2% "142,786" 43.02 L "Jax #1299 used in cross to Cast/Ei, no pupil response in R eye, normal in Left" Jun-19-95 15 "Box 45, Slot P-1" "12,876 x" 189 x DR DR "5,125 mm^2" "5,380 mm^2" 32.5 µm^2 0.59% 4400 26 363 14.0 ±5.02 ±1.00 11 8 10 8 15 14 15 13 15 1 10 15 22 11 18 13 24 11 21 11 10 17 15 17 19 19 C57BLKS/J-Bst/+ R F 5 062892A "9,826" "9,826" "±2,275" 23.2% "17,560" 55.96 R 920514 920628 45 18.0 1 two very small optic nerves RON: more normal nerve Nov-5-92 "Box 16, Slot J" "18,932 x" 370 x DR DR "2,407 mm^2" "5,380 mm^2" 15.0 µm^2 0.85% 1170 10 84 8.4 ±5.83 ±1.94 38198 40765 106.7% " 2,567 " 10 12 13 11 11 0 2 17 0 8 C57BLKS/J-Bst/+ R F 5 072993E "39,549" "39,549" "±2,445" 6.2% "58,045" 68.14 930601 930729 58 1 The left side was affected RON: more normal nerve Nov-2-93 B4 "Box 23, Slot D-4" "13,215 x" 252 x TK TK and RW "3,689 mm^2" "5,396 mm^2" 30.9 µm^2 1.01% 1879 19 400 21.1 ±5.52 ±1.30 38198 40765 106.7% " 2,567 " 18 19 31 20 19 21 22 24 22 15 33 23 8 18 16 19 25 24 23 C57BLKS/J-Bst/+ L F 5 111692D right/left average "44,383" "±1,596" "43,421" "±2,191" 5.0% "99,541" 43.62 JR0516 L 911224 921117 329 30.5 NA 1 "Normal pupillary light reflex, myelin unraveling" <5% unmyelinated "necrotic in #19,11,14,21,43,4" Feb-15-93 C3 "Box 21, Slot SÐ4" "13,086 x" 194 x DR DR "3,762 mm^2" "5,396 mm^2" 31.5 µm^2 1.36% 3159 43 591 13.7 ±4.49 ±0.69 45129 43813 97.1% " 1,316 " 20 15 8 26 14 12 10 19 9 11 14 11 20 16 12 18 17 12 10 4 21 8 14 11 13 13 14 15 6 13 16 13 18 19 11 17 10 8 13 8 19 15 18 C57BLKS/J-Bst/+ R 111692D "45,472" "±2,331" 5.1% "99,657" 45.63 JR0516 R 911224 921117 329 dup myelin unraveling <5% unmyelinated "necrotic sites in 36,37,44,50,5,10,13,15,17,19,20." Feb-15-93 C4 "Box 21, Slot TÐ3" "13,073 x" 194 x DR DR "3,766 mm^2" "5,396 mm^2" 31.6 µm^2 1.17% 3157 37 533 14.4 ±4.43 ±0.74 45415 47759 105.2% " 2,344 " 24 15 16 6 10 16 11 20 23 18 19 15 18 13 10 11 12 11 12 14 10 12 11 14 16 12 20 20 18 12 19 13 6 21 12 9 14 C57BLKS/J-Bst/+ L 5 120694E "33,492" "±4,733" 14.1% "57,472" 58.27 L 941206 Marginal fixation. Denny notes that count may be low. Rob reviewed case and the count looks solid to me. Good contrast in tissue and even unmyelinated fibers show up well. Lots of necrotic axons Aug-2-95 6 "Box 51, Slot G-1" 2 "13,018 x" 191 x DR DR and RW "2,096 mm^2" "5,380 mm^2" 31.7 µm^2 0.88% 1810 16 296 18.5 ±10.13 ±2.61 27 39 31 28 15 9 11 16 17 15 0 9 10 26 26 17 C57BLKS/J-Bst/+ R 120694E "8,396" "±1,474" 17.6% "35,008" 23.98 R 941206 Also marginal fixation Lots of necrotic axons Aug-2-95 7 "Box 51, Slot H-2" 2 "10,914 x" 245 x DR DR and RW "2,109 mm^2" "5,380 mm^2" 45.2 µm^2 1.55% 775 12 130 10.8 ±6.31 ±1.90 16 23 7 5 7 12 0 9 14 16 6 15 C57BLKS/J-Bst/+ L F 5 090993B "65,573" "±2,888" "64,633" "±3,951" 6.1% "114,246" L 930216 930909 205 29.0 Normal pupillary response Jun-22-95 C4 "Box 45, Slot N-1" "12,942 x" 141 x DR DR "2,262 mm^2" "5,380 mm^2" 32.1 µm^2 0.65% 3556 23 418 18.2 ±5.21 ±1.11 14 14 14 12 19 20 19 14 20 14 14 24 23 24 19 27 11 12 17 23 12 25 27 C57BLKS/J-Bst/+ R 090993B "66,652" "±4,231" 6.3% "120,133" R Jun-22-95 C5 "Box 45, Slot O-2" "12,947 x" 140 x DR DR "2,368 mm^2" "5,380 mm^2" 32.1 µm^2 0.56% 3742 21 374 17.8 ±5.06 ±1.13 12 13 7 16 13 26 23 22 23 17 12 21 18 16 18 23 16 12 20 22 24 C57BLKS/J-Bst/+ R M 5 011794G "63,321" "63,321" "±3,405" 5.4% "154,751" 40.92 R 930408 940117 284 28.0 "JAX #1307 R2, L+; pupil response does not agree with ON phenotype" Jun-19-95 17 "Box 26, Slot P-1" "12,837 x" 196 x DR DR "5,922 mm^2" "5,380 mm^2" 32.7 µm^2 1.05% 4740 50 668 13.4 ±5.03 ±0.72 6 6 12 15 14 15 11 12 18 16 9 20 22 9 6 10 20 27 16 16 5 16 9 19 12 9 12 17 14 13 17 11 19 12 14 6 7 17 11 12 15 6 19 11 20 18 10 21 7 9 15 8 15 13 15 10 24 C57BLKS/J-Bst/+ 011794G "1,923" ±631 32.8% "11,722" 1570 18.4% L Jul-8-95 16 "Box 26, slot O-4" "12,668 x" 187 x DR DR 412 mm^2 "5,380 mm^2" 33.5 µm^2 1.72% 350 6 33 5.5 ±4.04 ±1.81 13 4 2 3 7 4 C57BLKS/J-Bst/+ L F 5 011594A "52,834" "52,834" "±4,343" 8.2% "64,326" 82.13 931214 940115 32 13.0 from 1290 x 1297 or 1288) Apr-5-94 19 "Box 26, SlotM-2" "12,940 x" 194 x DR DR "2,423 mm^2" "5,380 mm^2" 32.1 µm^2 1.15% 2002 23 607 26.4 ±10.17 ±2.17 44 24 37 27 27 34 23 36 21 38 8 31 12 41 29 31 27 24 1 23 20 23 26 C57BLKS/J-Bst/+ R 011594A "13,057" "13,057" "±1,656" 12.7% "25,173" "12,940 x" 250 x DR DR "1,570 mm^2" "5,380 mm^2" 32.1 µm^2 1.15% 783 9 150 16.7 ±5.98 ±2.11 20 21 20 15 10 17 27 9 11 C57BLKS/J-Bst/+ F 5 052093C "9,319" "9,319" ±914 9.8% "29,754" ? 930520 both ONs from this case are abnormal Jul-8-95 A-2 "Box 23, Slot F-2" "12,668 x" 187 x DR DR "1,045 mm^2" "5,380 mm^2" 33.5 µm^2 0.90% 888 8 84 10.5 ±2.73 ±1.03 13 14 13 10 7 7 9 11 C57BLKS/J-+/+ R F 030894D "70,847" "±5,064" "±11,323" 6 "67,201" "±2,070" "69,446" "±3,116" 4.5% "120,574" 57.60 930818 940308 202 28.0 "UT#( from 1280 x 1356). Animal #9. HRP injection case. Listed as female in Denny's notes of injection. She was mated and had litter of 3 progeny, all Bst/+ on 1/2/94." Jun-22-95 6 "Box 45, Slot, M-2" "13,023 x" 141 x DR DR "2,408 mm^2" "5,380 mm^2" 31.7 µm^2 0.58% 3801 22 402 18.3 ±3.76 ±0.82 1.6436E-02 2.4458E-07 20 14 19 17 25 22 20 18 16 24 15 25 20 11 22 15 17 18 17 18 16 13 C57BLKS/J-+/+ L 030894D area of nerve "65,427" "±2,769" 4.2% "106,381" 61.50 Jun-24-95 5 "Box 45, Slot T-1" "12,894 x" 192 x DR DR "3,912 mm^2" "5,380 mm^2" 32.4 µm^2 0.64% 3287 21 418 19.9 ±3.77 ±0.84 11 20 19 21 20 18 22 27 19 18 20 23 20 20 18 16 29 23 20 16 18 C57BLKS/J-+/+ R or L M 072993G "90,080" "±6,521" "±18,443" 9 "74,547" "±3,918" "73,835" "±4,878" 6.6% "80,720" 91.47 L or R 930626 930729 33 23.0 "Jax #1372 (normal pupillary response), many unmyelinated axons in both optic nerves" Jun-19-95 C2 "Box 45, Slot L-1" "12,896 x" 190 x DR DR "2,921 mm^2" "5,380 mm^2" 32.4 µm^2 0.88% 2495 22 651 29.6 ±8.96 ±1.95 5.0883E-03 6.8257E-08 30 22 20 31 21 21 17 25 25 15 28 29 39 34 38 38 43 43 32 42 19 39 C57BLKS/J-+/+ L or R 072993G "75,840" "±6,576" 8.7% "88,635" R or L Other nerve Jun-24-95 C1 "Box 45, Slot R-1" "12,877 x" 193 x DR DR "3,296 mm^2" "5,380 mm^2" 32.4 µm^2 0.77% 2732 21 583 27.8 ±10.77 ±2.41 14 29 20 24 19 15 19 27 27 28 42 30 18 17 28 19 43 52 47 31 34 C57BLKS/J-+/+ M 062692E "82,621" "±4,641" "82,621" "±4,641" 5.6% "102,341" 80.73 GET Count JR0516 ? unknown 920627 100 26.0 NA 0 "Strain origin from C57BL/6J: from Biesle in 1947 to Kaliss in 1948, back to Jackson in 1948. Carries H-2 due to DBA/2J genetic contamination " Feb-3-93 A1 "Box 21, Slot IÐ3" "12,931 x" 192 x DR DR "3,757 mm^2" "5,283 mm^2" 27.3 µm^2 1.12% 3747 42 926 22.0 ±7.93 ±1.24 3.9301E-03 4.7567E-08 78280 85187 108.8% " 6,907 " 24 33 22 15 17 10 14 14 19 22 21 23 13 9 43 17 15 22 19 22 36 28 20 19 22 34 37 30 24 23 29 17 17 25 38 21 10 27 13 18 24 20 C57BLKS/J-+/+ M 062392C "69,906" "±4,348" "69,906" "±4,348" 6.2% "77,451" 90.26 JR0516 L 920514 920624 41 23.0 NA 0 Counted long ago by Denny. The original count did not allow unmyelinated axons to be resolve adequately. This is a new count by Denny. Rob counted unmyelinated and got same values as Denny (on average). Jax 1209. "This is a supposedly normal male littermate to numerous mutant females. Injected by Denny at JAX. Denny's notes say that the fixation was poor, but looks just fine. <10% unmyelinated" 1 necrotic on 0026 Jul-8-95 D3 "Box 16, Slot R-1" 4 "12,821 x" 188 x DR DR "2,723 mm^2" "5,406 mm^2" 32.9 µm^2 0.93% 2355 22 653 29.7 ±8.46 ±1.85 3.8745E-03 5.5424E-08 49562 51927 104.8% " 2,366 " 36 36 37 44 23 28 38 43 41 10 19 30 25 27 25 30 23 24 23 27 37 27 C57BLKS/J-+/+ L F 012693H right/left average "50,993" "±1,840" "53,065" "±3,250" 6.1% "82,697" 64.17 JR0516 L 930126 NA 1 This is the mother of the exencephalic cases. Did not have belly spot or kinky tail. Mating was Bst/+ male to this +/+ female. Myelin looks like it is unraveling in this case. <5% unmyelinated. Brain cut into pieces by Denny. Apr-14-93 C3 "Box 20A, Slot K-2" "13,004 x" 250 x DR DR "5,169 mm^2" "5,418 mm^2" 32.0 µm^2 1.32% 2581 34 699 20.6 ±7.23 ±1.26 1.5524E-02 3.0444E-07 56114 57578 102.6% " 1,464 " 30 3 26 11 23 17 19 13 14 18 19 17 17 19 9 29 26 24 24 30 7 19 18 21 25 24 29 26 13 29 27 31 28 14 C57BLKS/J-+/+ R 012693H "50,016" "±2,232" 4.5% "57,619" 86.80 JR0516 R 930126 dup <5% unmyelinated Apr-14-93 C4 "Box 20A, Slot L-1" "13,142 x" 249 x DR DR "3,566 mm^2" "5,418 mm^2" 31.4 µm^2 1.42% 1837 26 708 27.2 ±6.07 ±1.21 47907 59429 124.1% " 11,522 " 32 26 26 27 29 28 35 31 28 39 29 19 30 21 32 16 34 18 24 19 38 28 28 26 27 18 C57BLKS/J-+/+ R M 091092D "79,812" "±5,429" "79,812" "±5,429" 6.8% "94,299" 84.64 "47,085 " JR0516 ? 911215 921109 330 443 16.2 0 <10% unmyelinated. Fixed with 4% paraformaldehyde only. Optic nerves then postfixed in 1/4 Karnovsky fix. Small piece of cerebellum missing. This animal is probably from the Park colony A1 "Box 21, Slot R-3" "13,589 x" 199 x DR DR and RW "3,734 mm^2" "5,043 mm^2" 27.3 µm^2 1.01% 3453 35 809 23.1 ±9.17 ±1.57 2.7877E-03 3.4929E-08 79599 83779 105.3% " 4,180 " 14 22 24 25 18 32 35 21 20 11 18 17 15 30 37 30 40 29 37 24 0 36 29 18 23 10 9 19 20 35 26 23 29 17 16 C57BLKS/J-mea m +/+ db (meandertail-J) original F 5 900308-127 "43,860" "±1,243" "44,596" "±1,685" 3.8% "48,117" 92.68 JR1192 127 L 900308 GET NA 1 Case 127 Mar-1-91 C1 "Box 4, Slot I-1" "11,061 x" 386 x DT 2-18-91 DT and DT "7,169 mm^2" "5,105 mm^2" 41.7 µm^2 2.51% 1153 29 1121 38.7 ±7.73 ±1.46 2.9394E-02 6.7018E-07 46392 43286 93.3% " 3,106 " 45 40 37.4 26 41 35 38 54 48 46 34 37 38 27 37 50 54 47 34 36 34 36 42 33 42 24 37 27 42 C57BLKS/J-mea m +/+ db (meandertail-J) replicate 900308-127 "42,980" "±1,842" 4.3% "48,101" 89.35 JR1192 127 R 900308 GET dup Case 127 Mar-1-91 C2 "Box 4, Slot O-2" "9,863 x" 367 x DT 2-20-91 DT and DT "6,479 mm^2" "5,151 mm^2" 52.9 µm^2 3.19% 908 29 1372 47.3 ±10.73 ±2.03 44212 43182 97.7% " 1,030 " 71 44 30 38 53 33 44 50 54 56 52 57 50 53 65 52 54 44 61 41 40 42 44 46 52 52 40 34 20 C57BLKS/J-mea m +/+ db (meandertail-J) 5 900308-128 "58,354" "±2,932" "58,354" "±2,932" 5.0% "63,372" 92.08 JR1192 128 L 900308 GET NA dup Case 128 Mar-1-91 C3 "Box 4, Slot Q-2" "11,076 x" 386 x DT 2-20-91 DT and RW "9,442 mm^2" "5,163 mm^2" 42.1 µm^2 1.86% 1506 28 1085 38.8 ±10.12 ±1.95 7.0401E-03 1.2065E-07 59591 57117 95.8% " 2,474 " 22 36 26 36 15 20 36 38 40 35 43 61 28 43 46 40 38 43 43 42 54 41 57 43 43 36 40 40 C57BLKS/J-mea m +/+ db (meandertail-J) F 5 900927G-134 "39,268" "±1,436" "39,268" "±1,436" 3.7% "55,673" 70.53 JR1192 134 R 900927 GET NA Case 134. Mar-1-91 A6 "Box 8, Slot I-1" "8,099 x" 377 x DT 10-12-90 JO and DT "7,913 mm^2" "5,250 mm^2" 80.0 µm^2 3.16% 696 22 1242 56.5 ±9.46 ±2.06 1.9951E-02 5.0807E-07 37371 41165 110.2% " 3,794 " 67 66 65 70 55 52 38 67 63 54 61 50 52 63 48 63 41 65 54 41 47 60 C57BLKS/J-mea m +/+ db (meandertail-J) F 5 900206 "54,738" "±2,772" "54,738" "±2,772" 5.1% "78,985" 69.30 JR1192 62 L 900206 NA 1 Mar-1-91 D3 "Box 11, Slot E-1" "11,339 x" 394 x DT 2-15-91 DT and DT and RW "12,261 mm^2" "5,179 mm^2" 40.3 µm^2 1.78% 1961 35 977 27.9 ±8.24 ±1.41 7.3310E-03 1.3393E-07 54329 55124 101.5% 795 6 19 28 33 28 33 30 27 27 30 21 23 32 33 42 30 39 27 13 24 40 25 38 31 18 20 16 30 42 32 21 24 40 30 25 CAF1/J M 1 3 041493K "56,147" "±1,794" "±4,010" 6 "55,577" "±3,057" "55,577" "±3,057" 5.5% "75,092" 74.01 -0.142 JR100003 ? 468 930308 930413 36 21.3 see notes 0 1 Cross of BALB/cJ mother and A/J male. Brain weight recorded as 0.309 gm. "none noted, checked twice" Jun-2-93 C6 "Box 21.5, Slot T-3" 2 "13,039 x" 189 x DR RW and RW "2,674 mm^2" "5,371 mm^2" 31.6 µm^2 1.22% 2377 29 678 23.4 ±6.81 ±1.29 6.1581E-03 1.1080E-07 59638 58009 97.3% " 1,629 " 25 33 29 20 18 22 17 23 15 16 39 17 18 27 28 More to right 22 25 17 23 26 29 33 27 27 37 13 15 19 18 CAF1/J original F 1 3 041393K "51,815" "±2,435" "48,397" "±2,987" 6.2% "65,007" 74.45 -1.080 JR100003 ? 930308 930413 36 19.0 see notes 1 1 Cross of BALB/cJ and A/J. Brain weight (mis)recorded as 0.287 gm. May-27-93 B2 "Box 21.5, Slot Q-6" 2 "13,043 x" 185 x DR RW and RW "2,233 mm^2" "5,402 mm^2" 31.8 µm^2 1.37% 2047 28 662 23.6 ±7.58 ±1.46 9.0603E-03 1.7486E-07 26 30 16 9 22 19 23 28 29 30 19 26 27 22 5 26 26 37 26 22 6 30 28 35 28 21 25 21 CAF1/J replicate F 041393K "58,588" "±4,205" 7.2% "64,947" 90.21 Cross of BALB/cJ and A/J. Brain weight recorded as 0.287 gm. Reshot by Denny Rice to confirm initial estimate. 1 necrotic fiber Dec-12-94 B2 "Box 21.5, Slot Q-6" "16,194 x" 195 x DR RW and RW "2,457 mm^2" "5,418 mm^2" 20.7 µm^2 0.70% 3144 22 410 18.6 ±6.13 ±1.34 59730 57444 96.2% " 2,286 " 20 26 20 25 23 22 22 22 22 6 21 13 21 21 11 18 5 29 20 15 16 12 CAF1/J original M 1 3 012094C "62,614" "±3,222" "62,785" "±4,186" 6.7% "63,979" 98.13 1.612 JR100003 ? 931215 940120 36 22.0 473 0 2 Cross of BALB/cJ and A/J none Apr-8-94 14 "Box 26, Slot G-1" 4 "15,999 x" 194 x TK TK and RW "2,400 mm^2" "5,418 mm^2" 21.2 µm^2 0.73% 3022 22 457 20.8 ±6.35 ±1.38 6.3183E-03 1.0091E-07 65450 57639 88.1% " 7,811 " 16 17 19 33 29 21 12 22 24 22 22 32 21 10 20 17 25 22 19 26 7 21 CAF1/J replicate 0.00001 012094C "62,365" "±5,048" 8.1% "62,063" 100.49 Replicate of same section. Rob interleaved the second sample. Apr-17-95 14 "Box 26, Slot G-1" "13,039 x" 192 x RW RW and RW "2,300 mm^2" "5,270 mm^2" 31.0 µm^2 1.00% 2002 20 623 31.2 ±10.99 ±2.52 28 23 43 39 40 34 28 50 26 35 30 35 47 40 16 28 13 7 34 27 CAF1/J M 1 3 012094D "56,374" "±3,798" "56,374" "±3,798" 6.7% "78,820" 71.52 0.057 JR100003 ? 931215 940120 36 23.3 477 0 2 Cross of BALB/cJ and A/J none Apr-11-94 16 "Box 26, Slot H-2" 4 "12,944 x" 194 x TK TK and RW "2,957 mm^2" "5,440 mm^2" 32.5 µm^2 1.11% 2428 27 627 23.2 ±7.98 ±1.56 4.0584E-03 7.1991E-08 54051 62243 115.2% " 8,192 " 22 13 30 13 30 15 19 16 17 22 33 25 13 36 14 30 14 35 26 20 22 42 22 30 25 18 25 CAF1/J F 1 3 012094E "58,238" "±3,626" "58,238" "±3,626" 6.2% "85,000" 68.52 0.522 JR100003 ? 931215 940120 36 17.5 468 1 2 Cross of BALB/cJ and A/J none Apr-12-94 17 "Box 26, Slot I-1" 4 "12,954 x" 193 x TK TK and RW "3,169 mm^2" "5,440 mm^2" 32.4 µm^2 1.07% 2622 28 622 22.2 ±7.19 ±1.38 4.5930E-03 7.8865E-08 59155 62279 105.3% " 3,124 " 17 23 22 0 17 24 17 22 15 28 25 23 30 21 25 11 37 22 19 26 24 30 24 21 33 27 14 25 CAF1/J F 1 3 012094F "52,263" "±2,875" "52,263" "±2,875" 5.5% "74,091" 70.54 -0.968 JR100003 ? 931215 940120 36 17.3 454 1 2 Cross of BALB/cJ and A/J 1 necrotic Apr-12-94 20 "Box 26, Slot J-2" 4 "12,951 x" 193 x TK TK and RW "2,762 mm^2" "5,440 mm^2" 32.4 µm^2 1.09% 2284 25 572 22.9 ±6.17 ±1.26 6.5852E-03 1.2600E-07 56056 53913 96.2% " 2,143 " 35 19 21 18 27 29 17 30 26 23 25 33 25 21 24 11 19 16 31 22 17 20 28 23 12 CARL/RpGo original F 1 4 900927H "51,263" "±2,214" "±7,000" 11 "62,844" "±2,100" "64,146" "±2,481" 3.9% "66,726" 96.13 1.654 "Mus caroli established from breeders caught in Thailand by JT Marshall and sent to V. Chapman at Roswell Park Memmorial, then to R. Mullen, then to D. Goldowitz. Maintained by random mating." R 425 900927 100 NA 1 1 All calculations redone by Rob Sept 1993 M. caroli outbred from D. Goldowitz May-9-91 B4 "Box 12, Slot M-2" 3 "10,997 x" 377 x DT DT and DT "9,494 mm^2" "5,366 mm^2" 44.4 µm^2 1.93% 1504 29 1237 42.7 ±8.73 ±1.65 1.4764E-02 2.3493E-07 61557 68374 111.1% " 6,817 " 59 48 62 43 36 55 39 47 34 47 33 39 49 47 28 44 35 38 33 37 32 54 43 28 39 49 47 47 45 CARL/RpGo replicate 0.00001 900927H "59,562" "±3,940" 6.6% "71,020" 83.87 "Replicate by Toya on different thin section.March 22, 1995" none Mar-22-95 B4 "Box 12, Slot M-4" "10,897 x" 192 x TK RW and RW "2,630 mm^2" "5,418 mm^2" 45.6 µm^2 1.22% 1557 19 727 38.3 ±10.74 ±2.53 wrong mag 50 43 50 32 18 19 31 38 29 39 25 40 47 47 28 50 49 44 48 CARL/RpGo F 1 4 900607 "54,854" "±2,469" "54,854" "±2,469" 4.5% "97,814" 56.08 0.513 "Mus caroli established from breeders caught in Thailand by JT Marshall and sent to V. Chapman at Roswell Park Memmorial, then to R. Mullen, then to D. Goldowitz. Maintained by random mating." 5 R 900607 100 NA 1 2 All calculations by RW M. caroli outbred from D. Goldowitz Apr-24-91 A1 "Box 11, Slot Q-2" 3 "10,867 x" 363 x DT DT and RW "12,888 mm^2" "5,324 mm^2" 45.1 µm^2 1.80% 2170 39 986 25.3 ±7.01 ±1.14 9.2352E-03 1.6836E-07 60309 56372 93.5% " 3,938 " 25 14 24 26 9 27 19 25 17 22 32 27 23 25 9 26 22 33 25 34 38 31 27 26 31 31 32 27 33 29 32 18 24 20 9 31 34 25 24 CARL/RpGo original F 1 4 "900927I, 092790I" "47,288" "±2,294" "45,776" "±2,800" 6.1% "95,616" 47.87 -0.568 "Mus caroli established from breeders caught in Thailand by JT Marshall and sent to V. Chapman at Roswell Park Memmorial, then to R. Mullen, then to D. Goldowitz. Maintained by random mating." L 900927 100 NA 1 3 This original samples taken at relatively low magnification. Montage redone. All mags double checked. M. caroli outbred from D. Goldowitz Oct-12-90 C4 "Box 8, Slot G-9" 3 "7,352 x" 359 x DT DT and RW "12,323 mm^2" "5,250 mm^2" 97.1 µm^2 2.23% 984 22 1023 46.5 ±13.03 ±2.84 9.4135E-03 1.9907E-07 50271 48627 96.7% " 1,643 " 45 75 55 45 40 48 35 28 76 57 45 52 40 39 29 48 57 39 36 35 62 37 CARL/RpGo replicate 0.00001 "900927I, 092790I" "50,376" "±4,001" 7.9% "104,302" 48.30 900927 dup "First count used too low a sample magnifcation. Count was 49,449 in area of 103,289 microns square. " M. caroli outbred from D. Goldowitz Oct-30-94 C4 "Box 8, Slot G-9" "12,833 x" 192 x DR RW and RW "3,832 mm^2" "5,418 mm^2" 32.9 µm^2 0.85% 3171 27 429 15.9 ±6.44 ±1.26 52922 48010 90.7% " 4,912 " 15 7 6 22 27 16 8 29 18 24 14 25 19 15 19 19 3 9 22 11 17 15 17 11 14 14 13 CARL/RpGo M 1 4 910115D "54,796" "±3,559" "54,796" "±3,559" 6.5% "45,232" 121.14 0.505 "Mus caroli established from breeders caught in Thailand by JT Marshall and sent to V. Chapman at Roswell Park Memmorial, then to R. Mullen, then to D. Goldowitz. Maintained by random mating." 647 L 911124 920115 52 NA 0 4 "First count used too low a sample magnifcation. Count was 46,534 in area of 38,257 microns square. Looks like low count due mostly to miscalibration of low mag montage. Area of nerve now estimated to be 47110 microns square." M. caroli outbred from D. Goldowitz One or two necrotic fibers Oct-30-94 A5 "Box 9, Slot K-2" 3 "16,116 x" 193 x DR RW and RW "1,683 mm^2" "5,418 mm^2" 20.9 µm^2 1.01% 2168 22 556 25.3 ±7.52 ±1.64 4.5316E-03 8.2700E-08 63445 50346 79.4% " 13,098 " 30 45 17 25 14 19 17 35 23 22 21 29 30 31 27 29 15 18 29 23 23 34 CARL/RpGo original M 1 4 910115C "39,546" "±1,589" "38,945" "±1,778" 4.6% "44,302" 87.91 -1.674 "Mus caroli established from breeders caught in Thailand by JT Marshall and sent to V. Chapman at Roswell Park Memmorial, then to R. Mullen, then to D. Goldowitz. Maintained by random mating." 648 R 911124 920115 52 NA 0 4 Magnification of samples is low. Fixation of unmyelinated fibers is not good. Susequently rephotographed at 16 K. M. caroli outbred from D. Goldowitz Jan-25-91 A3 "Box 9, Slot M-2" 3 "7,694 x" 370 x DT DT and DT and RW "6,065 mm^2" "5,292 mm^2" 89.4 µm^2 5.85% 496 29 2279 78.6 ±18.99 ±3.59 1.6230E-02 4.1040E-07 36530 39924 109.3% " 3,394 " 42 83 94 63 77 95 85 58 98 61 65 87 81 72 73 98 134 83 89 65 38 82 72 76 74 66 99 71 98 CARL/RpGo replicate 0.00001 910115C "41,921" "±3,534" 8.4% "44,460" 94.29 911124 920115 52 dup M. caroli outbred from D. Goldowitz Oct-30-94 A3 "Box 9, Slot M-2" "16,186 x" 193 x DR RW and RW "1,654 mm^2" "5,418 mm^2" 20.7 µm^2 1.02% 2150 22 429 19.5 ±7.53 ±1.64 41545 42130 101.4% 585 39 28 17 22 23 12 18 17 6 29 12 18 14 17 18 23 28 19 22 22 19 6 CARL/RpGo original M 1 4 910115E "43,549" "±1,277" "43,564" "±1,418" 3.3% "43,257" 100.71 -1.102 "Mus caroli established from breeders caught in Thailand by JT Marshall and sent to V. Chapman at Roswell Park Memmorial, then to R. Mullen, then to D. Goldowitz. Maintained by random mating." 651 L 911124 920115 52 NA 0 4 Negatives mislabeled 900115E. Fixed with a cacodylic acid Karnovsky fix by Richard. Stained 40 min in UA and 3 min in Pb. M. caroli outbred from D. Goldowitz Jan-28-91 B3 "Box 9, Slot O-2" 3 "8,099 x" 373 x DT DT and RW and RW "6,005 mm^2" "5,292 mm^2" 80.7 µm^2 5.22% 536 28 2275 81.3 ±13.74 ±2.64 2.7698E-02 6.3603E-07 40678 46724 114.9% " 6,046 " 83 87 92 80 86 71 88 96 72 95 78 96 103 93 58 82 67 91 71 76 81 84 71 100 59 43 86 86 CARL/RpGo replicate 0.00001 910115E "43,483" "±2,938" 6.8% "43,052" 101.00 911124 920115 52 dup 4 Reshot at higher magnification. M. caroli outbred from D. Goldowitz Oct-30-94 B3 "Box 9, Slot O-2" "16,195 x" 193 x DR RW and RW "1,598 mm^2" "5,418 mm^2" 20.7 µm^2 1.06% 2084 22 459 20.9 ±6.46 ±1.41 45850 41113 89.7% " 4,737 " 34 12 25 21 31 24 14 16 26 30 20 18 20 27 15 13 21 16 17 28 19 12 CARL/RpGo F 1 4 010695I "57,460" "±2,861" "57,460" "±2,861" 5.0% "99,769" 57.59 0.885 "Mus caroli established from breeders caught in Thailand by JT Marshall and sent to V. Chapman at Roswell Park Memmorial, then to R. Mullen, then to D. Goldowitz. Maintained by random mating." 940329 950106 283 16.3 438 5 M. caroli outbred from D. Goldowitz Apr-12-95 17 "Box 42, Slot F-4" 2 "13,107 x" 193 x RW RW and RW "3,709 mm^2" "5,270 mm^2" 30.7 µm^2 0.74% 3252 24 424 17.7 ±4.22 ±0.88 7.3250E-03 1.2748E-07 23 25 16 15 22 16 17 19 20 17 19 13 27 17 20 10 15 17 19 19 18 8 17 15 CARL/RpGo F 1 4 010695K "57,455" "±4,216" "57,455" "±4,216" 7.3% "122,604" 46.86 0.885 "Mus caroli established from breeders caught in Thailand by JT Marshall and sent to V. Chapman at Roswell Park Memmorial, then to R. Mullen, then to D. Goldowitz. Maintained by random mating." 940329 950106 283 15.0 409 5 M. caroli outbred from D. Goldowitz Apr-12-95 21 "Box 42, Slot H-2" 2 "13,107 x" 193 x RW RW and RW "4,558 mm^2" "5,270 mm^2" 30.7 µm^2 0.80% 3997 32 460 14.4 ±5.87 ±1.05 3.3360E-03 5.8062E-08 3 12 15 16 2 16 15 15 16 10 12 5 25 12 9 11 14 9 7 18 17 15 15 20 19 20 28 15 20 21 19 9 CARL/RpGo M 1 4 012795A "46,936" "±3,649" "46,936" "±3,649" 7.8% "55,788" 84.13 -0.618 "Mus caroli established from breeders caught in Thailand by JT Marshall and sent to V. Chapman at Roswell Park Memmorial, then to R. Mullen, then to D. Goldowitz. Maintained by random mating." GET 18.3 381 6 M. caroli outbred from D. Goldowitz 1 necrotic fiber May-23-95 1 "Box 44, Slot Q-2" 3 "16,016 x" 248 x RCS RW and RW "3,434 mm^2" "5,270 mm^2" 20.5 µm^2 0.77% 2715 21 363 17.3 ±6.01 ±1.34 3.7018E-03 7.8868E-08 27 18 13 23 10 8 12 14 15 16 26 9 20 20 23 25 20 22 7 16 19 CARL/RpGo M 1 4 012795B "46,686" "±3,476" "46,686" "±3,476" 7.4% "70,022" 66.67 -0.654 "Mus caroli established from breeders caught in Thailand by JT Marshall and sent to V. Chapman at Roswell Park Memmorial, then to R. Mullen, then to D. Goldowitz. Maintained by random mating." GET 24.5 439 6 M. caroli outbred from D. Goldowitz 2 necrotic fibers May-23-95 4 "Box 44, Slot R-1" 3 "12,879 x" 247 x RCS RW and RW "4,278 mm^2" "5,270 mm^2" 31.8 µm^2 1.23% 2204 27 572 21.2 ±8.04 ±1.58 4.0121E-03 8.5938E-08 17 23 25 23 22 0 25 26 34 11 35 36 27 17 14 28 23 19 22 10 15 11 25 23 24 21 16 CARL/RpGo M 1 4 012795C "52,482" "±4,408" "52,482" "±4,408" 8.4% "118,132" 44.43 0.174 "Mus caroli established from breeders caught in Thailand by JT Marshall and sent to V. Chapman at Roswell Park Memmorial, then to R. Mullen, then to D. Goldowitz. Maintained by random mating." GET 26.3 423 6 M. caroli outbred from D. Goldowitz none May-23-95 5 "Box 44, Slot S-2" 3 "13,063 x" 193 x RCS and RW RW and RW "4,402 mm^2" "5,270 mm^2" 30.9 µm^2 0.65% 3825 25 343 13.7 ±5.65 ±1.15 2.8130E-03 5.3599E-08 9 23 14 15 20 17 7 9 17 11 21 20 14 3 19 13 8 6 7 23 10 12 13 20 12 CARL/RpGo bad fix F 010695J "Mus caroli established from breeders caught in Thailand by JT Marshall and sent to V. Chapman at Roswell Park Memmorial, then to R. Mullen, then to D. Goldowitz. Maintained by random mating." 940329 950106 283 18.8 457 5 M. caroli outbred from D. Goldowitz CAS32F1 F 081595Y 458 950707 950815 39 15.0 433 1 Cross between CAST/Ei and BXD32 Non-agouti coat color Tissue for DNA BXD32 #298 CAST/Ei #285 CAS32F1 F 081595Z 950707 950815 39 16.0 493 1 Cross between CAST/Ei and BXD32 Non-agouti coat color Tissue for DNA BXD32 #298 CAST/Ei #285 CAS32F1 F 081595AA 950707 950815 39 17.0 440 1 Cross between CAST/Ei and BXD32 Non-agouti coat color no BXD32 #298 CAST/Ei #285 CAS32F1 M 081595BB 950707 950815 39 16.0 455 1 Cross between CAST/Ei and BXD32 Non-agouti coat color no BXD32 #298 CAST/Ei #285 CAS32F1 M 081595CC 950707 950815 39 20.0 454 1 Cross between CAST/Ei and BXD32 Non-agouti coat color no BXD32 #298 CAST/Ei #285 CAS32F1 M 010496A 950705 960104 183 26.0 481 2 Breeder #326 Non-agouti coat color yes CAS32F1 M 010496B 950705 960104 183 26.5 483 2 Breeder #325 Non-agouti coat color yes CAS32F1 M 081595DD 950707 950815 39 13.0 424 1 Cross between CAST/Ei and BXD32 Non-agouti coat color no BXD32 #298 CAST/Ei #285 CAS32F2 M 101395A Case 377 non-agouti 424 950908 951013 35 18.0 430 1 #323 and #337 #325 CAS32F2 M 101395B Case 378 non-agouti 950908 951013 35 17.0 465 1 #323 and #337 #325 CAS32F2 M 101395C Case 362 non-agouti 950902 951013 41 19.8 447 1 #323 and #337 #325 CAS32F2 M 101395D Case 365 non-agouti 950902 951013 41 18.0 433 1 #323 and #337 #325 CAS32F2 F 101395E Case 379 Agouti 950908 951013 35 15.5 445 1 #323 and #337 #325 CAS32F2 M 101395F Case 380 Agouti 950908 951013 35 18.0 431 1 #323 and #337 #325 CAS32F2 F 101395G Case 363 Agouti 950902 951013 41 18.5 420 1 #323 and #337 #325 CAS32F2 M 101395H Case 364 Agouti 950902 951013 41 18.5 466 1 #323 and #337 #325 CAS32F2 F 101395I Case 381 Agouti 950908 951013 35 17.0 439 1 #323 and #337 #325 CAS32F2 M 101395J Case 382 Agouti 950908 951013 35 16.0 380 1 #323 and #337 #325 CAS32F2 M 112295A Case 415 Agouti 951013 951122 40 18.0 402 2 #318 or 319 #326 CAS32F2 M 112295B Case 416 Agouti 951013 951122 40 21.0 466 2 #318 or 319 #326 CAS32F2 F 112295C Case 417 Agouti 951013 951122 40 15.0 450 2 #318 or 319 #326 CAS32F2 F 112295D Case 418 non-agouti 951013 951122 40 14.8 407 2 #318 or 319 #326 CAS32F2 F 112295E Case 419 non-agouti 951013 951122 40 14.0 402 2 #318 or 319 #326 CAS32F2 F 112795A Case 420 non-agouti 951020 951127 38 11.0 420 3 #337 #328 CAS32F2 F 112795B Case 421 non-agouti 951020 951127 38 16.0 453 3 #337 #328 CAS32F2 F 112795C Case 422 Agouti 951020 951127 38 14.3 439 3 #337 #328 CAS32F2 F 112795D Case 423 Agouti 951020 951127 38 15.3 406 3 #337 #328 CAS32F2 F 112795E Case 424 Agouti 951020 951127 38 11.5 402 3 #337 #328 CAS32F2 M 121495A 951030 951214 45 18.5 409 4 Case 377 non-agouti #323 #325 CAS32F2 M 121495B 951030 951214 45 17.0 412 4 Case 378 non-agouti #323 #325 CAS32F2 M 121495C 951030 951214 45 19.0 466 4 Case 362 non-agouti #323 #325 CAS32F2 F 121495D 951030 951214 45 21.8 461 4 Case 365 non-agouti #323 #325 CAS32F2 F 121495E 951030 951214 45 15.5 445 4 Case 379 Agouti #323 #325 CAS32F2 F 121495F 951107 951214 37 10.8 382 5 Case 380 Agouti #318 #326 CAS32F2 F 121495G 951107 951214 37 16.3 401 5 Case 363 Agouti #318 #326 CAS32F2 F 121495H 951107 951214 37 13.5 412 5 Case 364 Agouti #318 #326 CAS32F2 M 121495I 951107 951214 37 17.5 496 5 Case 381 Agouti #318 #326 CAS32F2 F 121495J 951107 951214 37 16.5 429 5 Case 382 Agouti #318 #326 CAS32F2 F 121495K 951107 951214 37 14.5 432 5 Case 415 Agouti #318 #326 CAS32F2 M 121495L 951107 951214 37 16.5 436 5 Case 416 Agouti #318 #326 CAS32F2 M 121495M 951111 951214 33 18.5 388 6 Case 417 Agouti #337 #325 CAS32F2 M 121495N 951111 951214 33 17.0 444 6 Case 418 non-agouti #337 #325 CAS32F2 M 121495O 951111 951214 33 15.5 431 6 Case 419 non-agouti #337 #325 CAS32F2 M 121495P 951111 951214 33 14.5 407 6 Case 420 non-agouti #337 #325 CAS32F2 F 121495Q 951111 951214 33 18.0 416 6 Case 421 non-agouti #337 #325 CAS32F2 F 121495R 951111 951214 33 12.0 359 6 Case 422 Agouti #337 #325 CAS32F2 M 010496G 951123 960104 42 19.0 417 7 Case 452 non-agouti #319 #326 CAS32F2 F 010496H 951123 960104 42 14.5 390 7 Case 453 Agouti #319 #326 CAS32F2 M 010496I 951123 960104 42 18.5 411 7 Case 454 Agouti #319 #326 CAS32F2 M 010496J 951123 960104 42 18.5 435 7 Case 455 Agouti #319 #326 CAS32F2 M 010496K 951123 960104 42 20.0 428 7 Case 456 Agouti #319 #326 CAS32F2 M 010496L 951123 960104 42 16.0 434 7 Case 457 Agouti #319 #326 CAS32F2 F 010496M 951123 960104 42 18.5 403 7 Case 458 Agouti #319 #326 CAS32F2 F 010496N 951204 960104 31 14.5 445 8 Case 467 Agouti "#323,337" #325 CAS32F2 F 010496O 951204 960104 31 12.0 397 8 Case 468 Agouti "#323,337" #325 CAS32F2 M 010496P 951204 960104 31 15.5 444 8 Case 469 Agouti "#323,337" #325 CAS32F2 F 010496Q 951204 960104 31 13.5 420 8 Case 470 Agouti "#323,337" #325 CAS32F2 M 010496R 951204 960104 31 16.0 431 8 Case 471 Agouti "#323,337" #325 CAS32F2 F 010496S 951204 960104 31 15.0 442 8 Case 472 Agouti "#323,337" #325 CAS32F2 F 010496T 951204 960104 31 12.0 393 8 Case 473 non-agouti "#323,337" #325 CAS32F2 F 010496U 951204 960104 31 10.5 388 8 Case 474 Agouti "#323,337" #325 CAS32F2 M 010496V 951204 960104 31 17.0 440 8 Case 475 non-agouti "#323,337" #325 CAS32F2 M 010496W 951204 960104 31 13.0 468 8 Case 476 Agouti "#323,337" #325 CAS32F2 F 010496X 951204 960104 31 9.0 389 8 Case 477 Agouti "#323,337" #325 CAS32F2 F 010496Y 951204 960104 31 12.0 388 8 Case 478 non-agouti "#323,337" #325 CAS32F2 F 010496Z 951204 960104 31 10.0 448 8 Case 479 Agouti "#323,337" #325 CAS32F2 F 010496AA 951204 960104 31 11.0 424 8 Case 480 Agouti "#323,337" #325 CAS32F2 F 010496BB 951204 960104 31 10.0 387 8 Case 481 Agouti "#323,337" #325 CAS32F2 F 010496CC 951204 960104 31 10.0 426 8 Case 482 Agouti "#323,337" #325 CAS32F2 M 011296A 951203 960112 40 19.0 442 9 Case 460 Agouti #318 #326 CAS32F2 M 011296B 951203 960112 40 17.0 416 9 Case 461 Agouti #318 #326 CAS32F2 F 011296C 951203 960112 40 17.0 408 9 Case 462 non-agouti #318 #326 CAS32F2 M 011296D 951203 960112 40 18.0 412 9 Case 463 Agouti #318 #326 CAS32F2 F 011296E 951203 960112 40 18.0 417 9 Case 464 Agouti #318 #326 CAS32F2 F 011296F 951203 960112 40 18.0 454 9 Case 465 non-agouti #318 #326 CAS32F2 F 011296G 951203 960112 40 14.0 384 9 Case 466 non-agouti #318 #326 Case 423 Agouti Case 424 Agouti CAS32F2 CASA/Rk M 1 4 051293A "48,917" "±2,958" "±5,123" 4 "56,171" "±3,552" "56,171" "±3,552" 6.3% "71,097" 79.01 1.416 JR0735 JAX 4269 ? 342 930408 930512 34 10.0 342 0 1 none noted Jun-2-93 A1 "Box 25.5, Slot I-1" 2 "13,178 x" 188 x DR RW and RW "2,518 mm^2" "5,381 mm^2" 31.0 µm^2 1.26% 2294 29 710 24.5 ±8.19 ±1.55 4.6108E-03 8.2085E-08 55417 61971 111.8% " 6,554 " 32 31 35 12 22 23 28 15 12 31 24 16 41 28 28 20 37 32 32 31 25 27 20 16 15 30 15 11 21 CASA/Rk please reshoot 1E-08 051293A CASA/Rk F 1 4 051293B "45,131" "±2,335" "45,131" "±2,335" 5.2% "42,005" 107.44 -0.739 JR0735 JAX 4268 ? 930408 930512 34 9.5 317 1 1 Low mag calibration for other set photographed the same day none noted Jun-2-93 A4 "Box 25.5, Slot J-2" 2 "13,178 x" 188 x DR RW and RW "1,487 mm^2" "5,381 mm^2" 31.0 µm^2 1.25% 1356 17 566 33.3 ±6.89 ±1.72 8.7961E-03 1.9490E-07 47253 46047 97.4% " 1,206 " see to right 41 40 34 36 25 30 44 46 27 31 26 21 37 33 28 33 34 CASA/Rk F 1 4 051394M "48,878" "±3,624" "48,878" "±3,624" 7.4% "59,332" 82.38 -0.008 JR1145 ? 940307 940513 67 9.8 361 1 2 This is almost certainly a CASA/Rk. Mix up at JAX or here. Cases were mixed up with 072994F and G. Note body weight and brain weights revealed error. none Aug-11-94 16 "Box 27, Slot Q-2" 4 "13,073 x" 250 x RW RW and RW "3,712 mm^2" "5,443 mm^2" 31.9 µm^2 1.13% 1863 21 551 26.2 ±8.70 ±1.95 3.9078E-03 7.9949E-08 53117 53265 100.3% 147 20 11 28 12 32 27 14 24 23 30 22 29 24 38 21 26 26 26 32 39 47 CASA/Rk F 1 4 051394N "45,487" "±2,935" "45,487" "±2,935" 6.5% "48,819" 93.17 -0.670 JR1145 ? 940307 940513 67 9.5 348 1 2 This is almost certainly a CASA/Rk. Mix up at JAX or here. Cases were mixed up with 072994F and G. Note body weight and brain weights revealed error. Note large difference in brain weight. Identity of this mouse not certain. none Aug-11-94 18 "Box 27, Slot R-1" 2 "13,065 x" 249 x RW RW and RW "3,038 mm^2" "5,443 mm^2" 31.9 µm^2 1.37% 1531 21 624 29.7 ±8.57 ±1.92 5.5449E-03 1.2190E-07 48149 48727 101.2% 578 30 28 31 27 35 27 26 16 3 37 38 33 43 32 33 37 32 38 25 24 29 CAST/Ei F 1 4 091093A "45,484" ±999 "±3,160" 11 "44,911" "±4,374" "44,911" "±4,374" 9.7% "77,344" 58.07 -0.182 JAX 4436 ? 373 930604 931009 127 13.8 361 1 1 Poor fixation according to notes. Listed once as weighing 23 gms. Seems much to high. Check weight. Sort of difficult to count none Mar-8-94 D2 "Box 25, Slot G-1" 2 "12,911 x" 248 x TK TK and RW "4,772 mm^2" "5,454 mm^2" 32.7 µm^2 1.06% 2364 25 475 19.0 ±9.06 ±1.85 2.4457E-03 5.4456E-08 51957 38332 73.8% " 13,625 " 37 16 18 16 20 23 15 27 14 23 8 18 27 25 13 16 17 23 33 13 30 4 8 0 31 CAST/Ei M 1 4 120893A "43,970" "±2,551" "43,970" "±2,551" 5.8% "79,227" 55.50 -0.479 JAX 4471 ? 930611 931208 180 362 0 2 half of brain missing. Half brain weights 0.181 gm Fine tissue none Mar-2-94 C1 "Box 25, Slot E-1" 4 "12,897 x" 250 x TK TK and RW "4,936 mm^2" "5,453 mm^2" 32.8 µm^2 1.08% 2417 26 473 18.2 ±5.28 ±1.06 7.0291E-03 1.5986E-07 44017 44579 101.3% 562 16 21 26 23 21 9 17 20 21 19 27 23 13 18 24 13 7 19 16 26 20 11 19 17 11 16 CAST/Ei F 1 4 120893B "41,341" "±3,274" "41,341" "±3,274" 7.9% "83,558" 49.48 -1.311 JAX 4075 ? 930124 931208 318 NA 1 3 Myelin unraveled. Count is ok despite poor fixation. Difficult to count one or two necrotic Mar-3-94 C2 "Box 25, Slot F-2" 2 "12,909 x" 250 x TK TK and RW "5,228 mm^2" "5,439 mm^2" 32.6 µm^2 1.05% 2560 27 436 16.1 ±6.52 ±1.28 4.0057E-03 9.6895E-08 40133 42434 105.7% " 2,301 " 22 14 14 24 14 3 19 17 22 13 16 29 17 20 8 17 19 10 9 13 27 23 1 14 18 19 14 CAST/Ei F 1 4 010695A "44,918" "±3,174" "44,918" "±3,174" 7.1% "92,114" 48.76 -0.179 ? 940713 950106 177 14.8 409 1 4 Breeder #13 from our colony none Apr-12-95 1 "Box 42, Slot I-1" 4 "13,107 x" 193 x RW RW and RW "3,424 mm^2" "5,270 mm^2" 30.7 µm^2 0.80% 3003 24 359 15.0 ±5.07 ±1.06 4.6529E-03 1.0359E-07 28 8 9 17 17 16 13 19 21 14 14 5 9 17 17 15 20 13 13 8 17 13 15 21 CAST/Ei F 1 4 010695B "50,045" "±4,762" "50,045" "±4,762" 9.5% "98,305" 50.91 1.443 ? 940713 950106 177 15.5 410 1 5 Breeder #14 from our colony Very large axons none Feb-21-95 3 "Box 39, Slot B-2" 4 "13,069 x" 194 x TK RW and RW "3,712 mm^2" "5,418 mm^2" 31.7 µm^2 0.65% 3099 20 323 16.2 ±6.70 ±1.54 2.3230E-03 4.6417E-08 46480 53607 115.3% " 7,127 " 7 16 20 12 15 14 15 11 18 13 20 19 27 22 32 7 16 18 3 18 CAST/Ei M 1 4 011795E "50,160" "±3,361" "50,160" "±3,361" 6.7% "77,341" 64.86 1.480 ? 941213 950117 35 11.0 357 0 6 "Direct from JAX, from colony maintained by Wendy Palateri." none Feb-28-95 21 Box 39 Slot H-2 3 "13,189 x" 193 x TK TK and TK "2,893 mm^2" "5,418 mm^2" 31.1 µm^2 0.81% 2483 20 404 20.2 ±5.90 ±1.35 4.6751E-03 9.3204E-08 51400 48917 95.2% " 2,483 " 16 14 10 21 9 20 24 19 16 30 28 17 17 22 28 20 21 18 28 26 CAST/Ei M 1 4 011795F "41,633" "±3,063" "41,633" "±3,063" 7.4% "71,093" 58.56 -1.219 ? 941213 950117 35 10.8 342 0 6 "Direct from JAX, from colony maintained by Wendy Palateri." Light contrast negatives. Accurate count. Large myelinated fibers. Very few unmyelinated fibers. 1 necrotic Mar-27-95 3 "Box 39, Slot I-1" 2 "12,986 x" 246 x RW RW and RW "4,303 mm^2" "5,270 mm^2" 31.2 µm^2 0.88% 2275 20 366 18.3 ±5.87 ±1.35 4.6724E-03 1.1223E-07 13 21 15 24 10 4 11 19 21 23 18 28 19 13 22 20 16 25 23 21 CAST/Ei F 1 4 011795G "44,528" "±4,104" "44,528" "±4,104" 9.2% "66,124" 67.34 -0.303 ? 941213 950117 35 9.3 330 1 6 "Direct from JAX, from colony maintained by Wendy Palateri." Light contrast negatives. Accurate count. Large myelinated fibers. Very few unmyelinated fibers. none Mar-27-95 5 "Box 39, Slot J-2" 2 "12,986 x" 246 x RW RW and RW "4,002 mm^2" "5,270 mm^2" 31.2 µm^2 1.09% 2116 23 484 21.0 ±9.10 ±1.94 2.7635E-03 6.2062E-08 6 16 17 33 22 23 22 33 19 31 21 17 21 12 22 13 10 23 18 13 24 49 19 CAST/Ei M 1 4 011795H "46,282" "±4,309" "46,282" "±4,309" 9.3% "93,031" 49.75 0.252 ? 941213 950117 35 7.0 321 0 6 "Direct from JAX, from colony maintained by Wendy Palateri." "3 necrotic (photos 8, 21, and 24)" Mar-29-95 7 "Box 39, Slot K-1" 3 "13,112 x" 191 x RW RW and RW "3,380 mm^2" "5,270 mm^2" 30.7 µm^2 0.79% 3035 24 366 15.3 ±6.81 ±1.42 2.6008E-03 5.6196E-08 30 20 16 16 14 26 16 15 18 19 14 1 15 17 8 4 13 9 21 3 12 21 19 19 CAST/Ei in progress M 081595FF 950413 950815 124 15.5 399 Breeder #285. CAST/Ei F 121995H 950415 951219 248 17.0 409 CAST/Ei F 121995I 950415 951219 248 16.5 398 CAST/Ei M 1 4 013095A "49,391" "±3,504" "49,391" "±3,504" 7.1% "77,978" 63.34 1.236 ? 940713 950130 201 16.0 393 0 5 Breeder #3 none Apr-19-95 11 "Box 42, Slot L-1" 4 "13,040 x" 192 x RW RW and RW "2,885 mm^2" "5,270 mm^2" 31.0 µm^2 0.76% 2516 19 373 19.6 ±5.91 ±1.39 4.2466E-03 8.5978E-08 19 18 25 19 25 17 30 25 18 25 14 18 19 25 9 14 24 22 7 CAST/Ei M 1 4 013095B "43,151" "±4,669" "43,151" "±4,669" 10.8% "92,618" 46.59 -0.739 ? 940713 950130 201 13.8 361 0 5 Breeder #15 Many huge axons. none Apr-19-95 13 "Box 42, Slot M-2" 4 "13,040 x" 192 x RW RW and RW "3,426 mm^2" "5,270 mm^2" 31.0 µm^2 0.84% 2988 25 361 14.4 ±7.65 ±1.56 2.0619E-03 4.7784E-08 18 15 25 16 20 11 24 15 21 5 27 15 7 19 23 10 16 4 12 10 7 28 5 3 5 CB6F1/J F 1 3 041393J "66,688" "±1,094" "±2,446" 6 "63,427" "±4,229" "63,427" "±4,229" 6.7% "72,295" "9,165" 87.73 -1.333 JR100007 ? 517 930223 930413 49 18.0 see notes 1 1 agouti with white spots on tail. Brain weight recorded as .287 gm. Must be error? Cross BALB/cJ female by C57BL/6J male. It is possible that the male was actually a C57BL/6JAx1 type. May-26-93 A5 "Box 21.5, Slot PÐ1" 4 "13,203 x" 240 x DR RW and RW "4,165 mm^2" "5,387 mm^2" 31.1 µm^2 1.37% 2328 32 872 27.3 ±10.12 ±1.82 3.6613E-03 5.7725E-08 68264 62851 92.1% " 5,413 " 26 26 29 22 35 31 48 46 26 42 40 19 34 39 12 34 25 30 32 20 16 26 29 11 8 25 11 21 27 39 20 23 CB6F1/J please reshoot 1E-08 041393J CB6F1/J F 1 3 041393I "69,002" "±3,899" "69,002" "±3,899" 5.7% "80,370" "6,273" 85.86 0.946 JR100007 ? 930223 930413 49 18.0 see notes 1 1 agouti with white spots on tail. Brain weight recorded as .284 gm. Must be error? Cross BALB/cJ female by C57BL/6J male. It is possible that the male was actually a C57BL/6JAx1 type. May-26-93 A2 "Box 21. 5, Slot O-2" 4 "13,110 x" 240 x DR RW and RW "4,614 mm^2" "5,387 mm^2" 31.1 µm^2 1.28% 2588 33 880 26.7 ±8.52 ±1.51 4.6801E-03 6.7826E-08 68362 68258 99.8% 104 42 35 28 33 36 16 29 29 30 43 13 6 24 32 38 25 30 26 21 29 25 32 31 25 17 33 34 27 18 18 13 18 24 CB6F1/J original M 1 3 041493J "66,026" "±2,800" "61,111" "±4,346" 7.1% "79,114" "7,258" 77.24 -0.271 JR100007 ? 930222 930414 51 24.5 see notes 0 2 agouti with white spots on tail. Brain weight recorded as .335 gm. Must be error? Cross BALB/cJ female by C57BL/6J male. It is possible that the male was actually a C57BL/6JAx1 type. May-27-93 C2 "Box 21. 5, Slot S-4" 3 "13,038 x" 187 x DR RW and RW "2,773 mm^2" "5,387 mm^2" 31.7 µm^2 1.28% 2497 32 783 24.5 ±9.69 ±1.74 8.6963E-03 1.3171E-07 61963 65045 105.0% " 3,082 " 26 9 39 37 19 14 24 15 21 21 29 27 34 35 28 28 16 11 17 17 22 0 26 44 21 35 38 31 21 32 20 26 CB6F1/J replicate 0.00001 041493J "69,512" "±3,660" 5.3% "79,078" "11,051" 87.90 replicate of a different thin section none May-5-95 C2 "Box 21. 5, Slot S-4" "13,036 x" 193 x RCS EGW and RW "2,932 mm^2" "5,270 mm^2" 31.7 µm^2 0.84% 2495 21 585 27.9 ±6.56 ±1.47 21 21 26 32 25 28 23 17 24 36 30 36 36 32 39 21 26 19 33 24 36 CB6F1/J original M 1 3 041493I "70,088" "±2,614" "71,217" "±3,678" 5.2% "87,408" "7,905" 81.48 1.390 JR100007 ? 930222 930414 51 23.8 see notes 0 2 agouti with white spots on tail. Brain weight recorded as .351 gm. Must be error? Cross BALB/cJ female by C57BL/6J male. It is possible that the male was actually a C57BL/6JAx1 type. May-26-93 B6 "Box 21. 5, Slot R-5" 4 "13,140 x" 187 x DR RW and RW "3,053 mm^2" "5,387 mm^2" 31.2 µm^2 1.39% 2803 39 991 25.4 ±8.09 ±1.31 1.0525E-02 1.5016E-07 81609 68879 84.4% " 12,729 " 22 33 14 21 29 26 17 26 28 28 12 40 29 17 15 13 6 24 23 26 36 33 41 23 34 21 25 21 27 15 27 41 29 36 26 26 30 23 28 CB6F1/J replicate 0.00001 041493I "68,936" "±3,716" 5.4% "89,885" "13,565" 76.69 replicate of a different thin section none May-5-95 B6 "Box 21. 5, Slot R-5" "13,036 x" 193 x RCS EGW and RW "3,333 mm^2" "5,270 mm^2" 31.7 µm^2 0.81% 2836 23 559 24.3 ±6.15 ±1.31 15 27 27 22 18 18 15 22 33 25 33 33 23 17 29 29 21 23 27 17 32 33 20 CB6F1/J M 1 3 012094A "65,382" "±3,782" "65,382" "±3,782" 5.8% "105,563" "10,545" 61.94 -0.534 JR100007 ? 931201 940120 50 28.0 516 0 3 agouti. Reweighed by Toya Jan 95 at 0.4244 gm Cross BALB/cJ female by C57BL/6J male. It is possible that the male was actually a C57BL/6JAx1 type. No necrosis noted Apr-5-94 9 "Box 26, Slot E-1" 3 "12,957 x" 193 x TK TK and RW "3,951 mm^2" "5,437 mm^2" 32.4 µm^2 1.04% 3259 34 682 20.1 ±6.66 ±1.16 4.7103E-03 7.2043E-08 68120 64616 94.9% " 3,503 " 25 26 13 23 19 15 19 20 12 12 27 31 26 22 26 33 27 5 23 14 10 19 20 24 23 18 11 24 16 32 21 15 14 17 CB6F1/J M 1 3 012094B "66,204" "±3,685" "66,204" "±3,685" 5.6% "84,328" "11,397" 78.51 -0.198 JR100007 ? 931201 940120 50 24.5 518 0 3 agouti. Brain reweighed by Toya Jan 95 0.5162 gm. Cross BALB/cJ female by C57BL/6J male. It is possible that the male was actually a C57BL/6JAx1 type. none Apr-8-94 11 "Box 26, Slot F-2" 3 "12,924 x" 193 x TK TK and RW "3,146 mm^2" "5,438 mm^2" 32.6 µm^2 0.97% 2590 25 639 25.6 ±6.97 ±1.42 5.0773E-03 7.6692E-08 68628 70249 102.4% " 1,620 " 22 19 35 32 37 14 31 31 27 27 30 34 30 32 25 27 17 15 19 30 29 26 17 16 17 CBA/CaJ original M 1 1 041493C "56,028" "±1,145" "±2,561" 6 "54,168" "±3,219" "48,231" "±4,472" 9.3% "66,021" "6,890" 73.05 -0.726 JR000654 ? 440 930222 930414 51 18.8 see notes 0 1 There is no retinal degeneration (rd) in this CBA substrain. Brain weight mismeasured at 0.265 gm. none Oct-12-93 C5 "Box 22, Slot D-2" 3 "12,766 x" 247 x TK TK and RW "4,013 mm^2" "5,323 mm^2" 32.8 µm^2 1.29% 2013 26 623 24.0 ±11.11 ±2.22 5.4357E-03 1.0035E-07 53347 51166 95.9% " 2,181 " 35 28 21 33 24 35 26 29 34 33 18 8 34 38 36 14 18 32 3 31 35 19 3 13 18 5 CBA/CaJ replicate 0.00001 041493C "60,555" "±4,638" 7.7% "63,412" 95.49 Repeat by Rob. Low contrast tissue and negatives. Apr-19-95 C5 "Box 22, Slot D-2" "16,032 x" 192 x RW RW and RW "2,346 mm^2" "5,270 mm^2" 20.5 µm^2 0.61% 3093 19 372 19.6 ±6.36 ±1.50 18 17 13 24 12 19 11 21 25 17 26 24 16 31 25 20 17 7 29 CBA/CaJ M 1 1 041493D "55,749" "±3,064" "55,749" "±3,064" 5.5% "66,265" 84.13 -0.109 JR000654 ? 930222 930414 51 18.3 see notes 0 1 There is no retinal degeneration (rd) in this CBA substrain. Brain weight mismeasured at 0.255 gm. 1 necrotic thing Oct-12-93 C5 "Box 22, Slot G-1" 4 "12,715 x" 249 x TK TK and RW "4,113 mm^2" "5,391 mm^2" 33.3 µm^2 1.41% 1987 28 786 28.1 ±8.01 ±1.54 6.1577E-03 1.1045E-07 56239 63804 113.5% " 7,565 " 29 34 34 28 12.5 26 30 22 3 31 32 21 33 25 24 29 38 34 41 24 28 27 40 21 38 27 25 29 CBA/CaJ original F 1 1 041393C "52,809" "±2,551" "47,699" "±4,032" 8.5% "61,624" 77.40 -1.257 JR000654 ? 930223 930413 49 14.3 see notes 1 2 There is no retinal degeneration (rd) in this CBA substrain. Brain weight mismeasured at 0.245 gm. Low calibration double-checked by Rob. none Nov-17-93 B2 "Box 23, Slot G-1" 4 "13,199 x" 254 x TK TK and RW "3,981 mm^2" "5,372 mm^2" 30.8 µm^2 1.05% 1999 21 501 23.9 ±9.02 ±2.02 8.5195E-03 1.6133E-07 50755 51849 102.2% " 1,095 " 25 21 13 26 18 20 37 13 28 27 29 35 21 7 19 43 12.2 18 33 26 30 CBA/CaJ replicate 0.00001 041393C "56,221" "±3,295" 5.9% "65,701" 85.57 Repeat by Toya on different thin section. none Mar-23-95 B2 "Box 23, Slot G-1" "13,056 x" 193 x TK RW and RW "2,436 mm^2" "5,418 mm^2" 31.8 µm^2 0.97% 2067 20 544 27.2 ±6.95 ±1.59 32 36 35 38 30 20 28 19 17 22 27 29 36 29 16 26 23 23 21 37 CBA/CaJ F 1 1 041393D "56,279" "±3,118" "56,279" "±3,118" 5.5% "66,240" 84.96 0.098 JR000654 ? 930223 930413 49 14.0 see notes 1 2 There is no retinal degeneration (rd) in this CBA substrain. Brain weight mismeasured at 0.258 gm. none Nov-17-93 B6 "Box 23, Slot H-2" 4 "13,041 x" 247 x TK TK and RW "4,056 mm^2" "5,373 mm^2" 31.6 µm^2 0.91% 2097 19 510 26.8 ±6.31 ±1.49 6.1105E-03 1.0857E-07 59466 59737 100.5% 271 29 32 27 28 45 27 34 29 20 19 18 30 20 23 23 29 24 24 29 CBA/CaJ original F 1 1 112194B "60,281" "±3,227" "62,428" "±4,852" 7.8% "61,934" 100.80 1.661 JR000654 ? 941017 941121 35 16.5 451 1 3 Agouti none Feb-2-95 3 "Box 37, Slot K-2" 4 "13,136 x" 196 x TK RCS and RW "2,374 mm^2" "5,418 mm^2" 31.4 µm^2 1.01% 1972 20 633 31.7 ±10.72 ±2.46 6.0951E-03 1.0111E-07 65483 59369 90.7% " 6,114 " 38 12 28 27 22 39 35 29 23 24 37 49 38 44 12 40 30 48 38 20 CBA/CaJ replicate 0.00001 112194B "58,579" "±4,320" 7.4% "63,748" 91.89 Repeat by Toya on different thin section. none Mar-23-95 3 "Box 37, Slot K-2" "13,091 x" 193 x TK RW and RW "2,364 mm^2" "5,418 mm^2" 31.6 µm^2 0.99% 2016 20 581 29.1 ±9.34 ±2.14 28 35 42 36 20 25 32 15 26 35 17 39 34 36 46 35 18 18 28 16 CBA/CaJ M 1 1 112194C "56,885" "±4,552" "56,885" "±4,552" 8.0% "60,208" 94.48 0.335 JR000654 ? 941017 941121 35 18.0 428 0 3 Agouti none Feb-2-95 6 "Box 37, Slot L-1" 4 "13,136 x" 196 x TK RCS and RW "2,308 mm^2" "5,418 mm^2" 31.4 µm^2 1.10% 1917 21 623 29.7 ±10.62 ±2.37 2.8821E-03 5.0665E-08 56651 57139 100.9% 488 26 30 49 22 23 38 11 35 39 39 25 24 11 37 39 16 39 41 33 16 30 CCASF1 original M 1 3 032394A "59,705" "±1,278" "±3,615" 9 "63,671" "±3,075" "68,537" "±5,422" 7.9% "74,131" 92.45 1.097 ? 489 940127 940323 55 23.3 484 0 1 "Father was a CAST/Ei, mother a BALB/c" 1 necrotic Jun-17-94 19 "Box 27, Slot E-1" 3 "12,982 x" 194 x TK TK and RW "2,792 mm^2" "5,408 mm^2" 32.1 µm^2 0.78% 2310 18 534 29.7 ±9.68 ±2.35 7.1281E-03 1.1195E-07 58782 78291 133.2% " 19,509 " 32 25 40 32 41 4 31 20 31 27 46 35 27 41 29 19 28 26 CCASF1 replicate 0.00001 032394A "61,364" "±3,734" 6.1% "70,370" 87.20 Duplicate analysis of a different thin section. May-15-95 19 "Box 27, Slot E-1" "13,072 x" 247 x RW RW and RW "4,290 mm^2" "5,270 mm^2" 30.8 µm^2 0.83% 2282 19 511 26.9 ±6.94 ±1.64 26 25 20 24 27 28 26 23 43 26 34 40 33 22 24 29 25 24 12 CCASF1 M 1 3 032394B "64,436" "±3,255" "64,436" "±3,255" 5.1% "100,218" 64.30 1.308 ? 940121 940323 61 26.5 496 0 1 "Father was a CAST/Ei, mother a BALB/c" none Jun-17-94 1 "Box 27, Slot C-1" 4 "12,994 x" 195 x TK TK and RW "3,811 mm^2" "5,438 mm^2" 32.2 µm^2 1.09% 3112 34 704 20.7 ±6.01 ±1.05 6.2679E-03 9.7274E-08 61141 67731 110.8% " 6,590 " 26 12 15 17 19 19 26 24 16 8 33 19 26 12 23 19 28 16 15 22 16 17 19 31 26 29 23 20 23 31 16 14 20 24 CCASF1 27.9 M 1 3 032394C "56,117" "±4,009" "56,117" "±4,009" 7.1% "99,637" 56.32 -0.992 ? 940121 940323 61 23.8 492 0 1 "Father was a CAST/Ei, mother a BALB/c" none Jun-17-94 3 "Box 27, Slot D-2" 2 "13,045 x" 195 x TK TK and RW "3,801 mm^2" "5,428 mm^2" 31.9 µm^2 0.90% 3124 28 503 18.0 ±6.67 ±1.28 3.6200E-03 6.4508E-08 48866 63369 129.7% " 14,504 " 36 14 17 4 12 4 23 22 22 14 26 18 20 18 25 21 15 17 18 19 25 9 9 19 18 21 18 19 CCASF1 M 1 3 042694E "61,796" "±4,665" "61,796" "±4,665" 7.5% "76,019" 81.29 0.578 ? 940125 940426 91 25.0 see notes 0 2 Excess F1 male not needed for breeding. Half missingÑBrain weight recorded as 0.213 gm Dec-22-94 3 "Box 35, Slot C-1" 3 "16,122 x" 193.17 x TK RW and RW "2,837 mm^2" "5,418 mm^2" 20.8 µm^2 0.49% 3647 18 305 16.9 ±5.27 ±1.28 3.0061E-03 4.8646E-08 54703 68885 125.9% " 14,182 " 21 13 14 17 15 17 18 14 12 22 23 13 28 20 25 10 14 9 CCASF1 F 1 3 090894K "57,790" "±5,615" "57,790" "±5,615" 9.7% "112,367" 51.43 -0.530 ? 940121 940908 230 27.5 473 1 1 half of brain missing Dec-7-94 3 "Box 30, Slot O-2" 4 "13,067 x" 193 x TK RCS and RW "4,186 mm^2" "5,418 mm^2" 31.7 µm^2 0.62% 3541 22 359 16.3 ±7.27 ±1.59 1.9200E-03 3.3223E-08 58271 57305 98.3% 966 11 11 15 19 25 18 19 20 26 7 2 4 6 18 20 16 21 21 12 15 24 29 CCASF1 R or L F 1 3 090894L "61,279" "±4,304" "60,510" "±6,123" 10.1% "119,948" 50.45 0.435 Right or left 940121 940908 230 28.5 516 1 1 Right or left nerve Brain weighed at 0.5162 gm by Toya. Original weight by Richard Cushing was 539 mg. Dec-7-94 6 "Box 34, Slot G-1" 4 "13,142 x" 193 x TK RCS and RW "4,468 mm^2" "5,418 mm^2" 31.4 µm^2 0.60% 3823 23 364 15.8 ±7.51 ±1.60 3.4579E-03 5.6429E-08 71369 49066 68.8% " 22,303 " 14 6 28 14 13 1 24 5 23 20 19 8 29 20 7 15 12 10 13 23 22 18 20 CCASF1 L or R 0.00001 090894L "62,030" "±6,052" 9.8% "120,861" 51.32 Left or right 940121 940908 dup 1 Other side Dec-7-94 5 "Box 33, Slot S-2" 4 "13,042 x" 193 x RCS RCS and RW "4,502 mm^2" "5,418 mm^2" 31.9 µm^2 0.61% 3794 23 376 16.3 ±7.48 ±1.59 73040 50016 68.5% " 23,024 " 22 3 27 15 30 3 20 21 10 9 20 14 22 14 13 14 14 20 20 7 8 25 25 CCASF1 original M 1 3 112194T "55,238" "±3,107" "54,460" "±5,769" 10.6% "51,444" 105.86 -1.236 ? 940405 941121 230 15.5 449 0 3 "Father was a CAST/Ei, mother a BALB/c. Agouti" none Dec-22-94 19 "Box 35, Slot J-2" 3 "16,122 x" 193.17 x TK RW and RW "1,920 mm^2" "5,418 mm^2" 20.8 µm^2 0.61% 2468 15 331 22.1 ±8.75 ±2.34 6.1319E-03 1.1101E-07 54294 54603 100.6% 308 20 28 26 9 14 18 7 25 11 24 30 27 36 23 33 CCASF1 replicate 0.00001 112194T "55,555" "±3,687" 6.6% "49,860" 111.42 Apr-21-95 19 "Box 35, Slot J-2" "12,907 x" 248 x RW RW and RW "3,076 mm^2" "5,270 mm^2" 31.6 µm^2 1.27% 1576 20 705 35.3 ±10.20 ±2.34 33 44 26 19 60 36 33 40 25 34 44 33 33 34 16 27 40 38 40 50 CCASF1 M 1 3 112194U "61,693" "±5,695" "61,693" "±5,695" 9.2% "75,879" 81.30 0.550 ? 940405 941121 230 15.5 454 0 3 "Father was a CAST/Ei, mother a BALB/c. Agouti" none Dec-22-94 21 "Box 35, Slot K-2" 4 "16,122 x" 193.17 x TK RW and RW "2,831 mm^2" "5,418 mm^2" 20.8 µm^2 0.52% 3640 19 322 16.9 ±6.64 ±1.56 2.0079E-03 3.2547E-08 68354 55694 81.5% " 12,660 " 13 25 16 20 23 29 7 22 25 17 12 23 15 17 8 20 6 14 10 CCASF1 in progress M 081595II 941024 950815 295 28.5 see notes: Massive brain #34. Father of many F2s. Brain weighed 560 mg. Extremely high and perhaps age related. Tissue for DNA CCASF1 F 091895I 941024 950918 329 27.5 541 #35 female breeder CCASF1 F 091895J 941111 950918 311 28.0 521 #59 female breeder CCASF1 M 1 3 010695O "55,328" "±4,912" "55,328" "±4,912" 8.9% "71,973" 76.87 -1.211 941122 950106 45 23.0 488 0 4 BALB/cJ females #4 and #5 by CAST/Ei male #3 Apr-21-95 9 "Box 42, Slot R-1" 2 "15,867 x" 249 x RW RW and RW "4,474 mm^2" "5,270 mm^2" 20.9 µm^2 0.64% 3438 22 354 16.1 ±6.55 ±1.43 2.4027E-03 4.3427E-08 16 5 26 11 12 22 28 18 16 25 25 15 12 9 17 13 9 12 26 11 15 11 CCASF1 Box 42 do it M 010695P 941122 950106 45 24.1 490 4 BALB/cJ females #4 and #5 by CAST/Ei male #3 CCASF1 "Box 42, do it" M 010695Q 941122 950106 45 23.0 469 4 BALB/cJ females #4 and #5 by CAST/Ei male #3 CCASF2/JGo F 1 6 072994E2 "56,617" "±1,447" "±8,683" 75 "61,956" "±5,669" "61,956" "±5,669" 9.2% "48,826" 126.89 0.615 ? 443 940630 940729 29 17.0 420 1 1 agouti (A/-). Progeny of intercross between F1 female #1 and F1 male #4. Weird fixation but good count. none Oct-5-94 9 "Box 30, Slot A-3" 2 "13,067 x" 249 x RC RC and RW "3,015 mm^2" "5,418 mm^2" 31.7 µm^2 0.97% 1539 15 604 40.3 ±13.79 ±3.68 2.0654E-03 3.3336E-08 65720 58659 89.3% " 7,061 " 47 42 30 79 32 51 35 43 45 34 40 14 40 36 36 CCASF2/JGo original M 1 6 072994D2 "53,611" "±3,223" "63,213" "±6,414" 10.1% "79,613" 79.40 -0.346 ? 940630 940729 29 16.3 477 0 1 Albino. Progeny of intercross between F1 female #1 and F1 male #4. none Nov-29-94 8 "Box 30, Slot B-4" 2 "13,021 x" 191 x RCS RW and RW "2,913 mm^2" "5,418 mm^2" 32.0 µm^2 0.64% 2491 16 406 25.4 ±9.97 ±2.57 5.5035E-03 1.0266E-07 64871 55955 86.3% " 8,916 " 22 28 24 32 32 9 15 43 17 18 15 25 43 37 21 25 CCASF2/JGo replicate 0.00001 072994D2 "50,366" "±3,728" 7.4% "76,591" 65.76 Replicate of different thin section. Poor preservation and contrast. This count may be an undercount. Mar-24-95 8 "Box 30, Slot B-4" "13,104 x" 193 x TK RW and RW "2,856 mm^2" "5,418 mm^2" 31.6 µm^2 0.82% 2427 20 415 20.8 ±6.70 ±1.54 25 28 21 30 23 2 19 26 25 21 16 19 29 21 25 20 8 18 18 21 CCASF2/JGo F 1 6 072994C2 "58,167" "±1,804" "58,167" "±1,804" 3.1% "53,164" 109.41 0.179 ? 940630 940729 29 16.0 459 1 1 Albino. Progeny of intercross between F1 female #1 and F1 male #4. none Nov-29-94 5 "Box 30, Slot C-5" 4 "13,051 x" 192 x RCS RW and RW "1,970 mm^2" "5,418 mm^2" 31.8 µm^2 0.90% 1671 15 522 34.8 ±4.04 ±1.08 1.9143E-02 3.2910E-07 58739 57664 98.2% " 1,074 " 34 31 35 35 28 31 36 43 32 37 35 35 33 34 43 CCASF2/JGo F 1 6 072994A2 "53,009" "±5,037" "53,009" "±5,037" 9.5% "70,617" 75.07 -0.415 ? 940630 940729 29 16.0 488 1 1 Albino F2 Female 103 or 105 from Cage 2 none Dec-22-94 2 "Box 35, Slot D-2" 3 "16,122 x" 193 x THK RW and RW "2,635 mm^2" "5,418 mm^2" 20.8 µm^2 0.50% 3388 17 266 15.6 ±5.95 ±1.49 2.2200E-03 4.1880E-08 49545 56085 113.2% " 6,540 " 19 21 21 9 10 11 21 17 25 15 15 22 13 12 9 4 22 CCASF2/JGo original M 1 6 072994B2 "51,192" "±2,881" "44,377" "±5,149" 11.6% "75,683" 58.64 -0.625 ? 940630 940729 29 12.3 444 0 1 Albino F2. Progeny of intercross between F1 female #1 and F1 male #4. Male 104 or 106 from Cage 2 1 necrotic on 0034 none Dec-22-94 4 "Box 35, Slot E-1" 3 "16,122 x" 193 x THK RW and RW "2,824 mm^2" "5,418 mm^2" 20.8 µm^2 0.50% 3631 18 220 12.2 ±5.85 ±1.42 6.5319E-03 1.2760E-07 37114 51637 139.1% " 14,523 " 14 25 8 18 10 14 9 5 13 1 9 11 11 16 6 17 12 21 CCASF2/JGo replicate 0.00001 072994B2 "54,297" "±3,476" 6.4% "74,821" 72.57 Replicate of different thin section. Mar-22-95 4 "Box 35, Slot E-1" "10,866 x" 194 x TK RW and RW "2,815 mm^2" "5,418 mm^2" 45.9 µm^2 1.23% 1631 20 666 33.3 ±9.29 ±2.13 37 38 30 33 21 24 32 27 21 43 34 29 30 34 35 32 31 38 32 65 CCASF2/JGo original M 1 6 062894A "65,693" "±3,813" "77,124" "±5,866" 7.6% "73,631" 104.74 1.045 ? 940605 940628 23 9.5 415 0 3 agouti (A/-) F2 From Cage 2 case 55 none Dec-20-94 8 "Box 34, Slot M-2" 4 "13,051 x" 193 x TK RW and RW "2,730 mm^2" "5,418 mm^2" 31.8 µm^2 0.82% 2315 19 633 33.3 ±10.75 ±2.53 4.7698E-03 7.2608E-08 81715 72018 88.1% " 9,697 " 37 19 35 48 44 34 23 21 30 29 58 26 27 39 38 22 19 42 42 CCASF2/JGo replicate 0.00001 062894A "57,772" "±5,018" 8.7% "72,344" 79.86 Replicate of different thin section. Large difference is genuine sampling variation. I have confirmed that this is the same nerve. 1 necrotic Mar-23-95 8 "Box 34, Slot M-2" "13,051 x" 193 x TK RW and RW "2,698 mm^2" "5,418 mm^2" 31.8 µm^2 0.88% 2274 20 508 25.4 ±9.62 ±2.21 19 20 31 33 33 43 21 18 26 19 18 17 27 40 32 42 9 27 13 20 CCASF2/JGo replicate 2 0.00001 062894A "65,193" "±5,320" 8.2% "73,165" 89.10 Second replicate of a different section to obtain more accurate estimate May-7-95 8 "Box 34, Slot M-2" "13,103 x" 193 x RCS EGW and RW "2,732 mm^2" "5,270 mm^2" 30.7 µm^2 0.84% 2384 20 547 27.4 ±9.73 ±2.23 22 21 28 35 19 17 25 20 37 18 39 33 39 31 40 32 20 9 18 44 CCASF2/JGo F 1 6 062894B "70,075" "±5,037" "70,075" "±5,037" 7.2% "60,370" 116.08 1.550 ? 940605 940628 23 10.0 443 1 3 agouti (A/-) F2 From Cage 2 case 56 none Dec-20-94 11 "Box 34, Slot N-1" 4 "13,051 x" 193 x THK RW and RW "2,239 mm^2" "5,418 mm^2" 31.8 µm^2 1.32% 1898 25 923 36.9 ±13.00 ±2.65 2.8776E-03 4.1064E-08 68327 71965 105.3% " 3,638 " 56 46 35 37 50 42 50 47 45 41 16 22 42 42 37 40 12 13 27 50 31 38 22 24 58 CCASF2/JGo F 1 6 062894C "73,720" "±5,639" "73,720" "±5,639" 7.6% "73,806" 99.88 1.970 ? 940605 940628 23 11.0 429 1 3 agouti (A/-) F2 From Cage 2 case 57 none none Dec-20-94 13 "Box 34, Slot O-2" 2 "16,258 x" 193 x RW RW and RW "2,758 mm^2" "5,418 mm^2" 20.5 µm^2 0.53% 3601 19 389 20.5 ±6.64 ±1.57 2.4468E-03 3.3191E-08 72413 74893 103.4% " 2,480 " 28 21 19 13 17 29 17 5 13 17 30 18 24 26 22 31 22 21 16 CCASF2/JGo F 1 6 062894D "54,563" "±5,570" "54,563" "±5,570" 10.2% "54,556" 100.01 -0.237 ? 940605 940628 23 10.8 411 1 3 agouti (A/-) F2 From Cage 2 case 58 1 necrotic none Dec-20-94 15 "Box 34, Slot P-3" 2 "16,258 x" 193 x RW RW and RW "2,039 mm^2" "5,418 mm^2" 20.5 µm^2 0.83% 2662 22 451 20.5 ±9.59 ±2.09 1.8423E-03 3.3765E-08 55892 53230 95.2% " 2,662 " 23 30 26 11 10 31 13 22 12 23 29 12 25 17 31 15 31 38 0 7 20 25 CCASF2/JGo F 1 6 062894E "54,948" "±5,782" "54,948" "±5,782" 10.5% "64,527" 85.15 -0.192 ? 940605 940628 23 11.8 440 1 3 agouti (A/-) F2 From Cage 2 case 59 none none Dec-23-94 3 "Box 35, Slot C-1" 2 "16,122 x" 193 x THK RW and RW "2,408 mm^2" "5,418 mm^2" 20.8 µm^2 0.52% 3096 16 284 17.8 ±7.23 ±1.87 1.7530E-03 3.1904E-08 53785 56107 104.3% " 2,322 " 26 16 4 23 22 30 22 23 21 6 19 10 13 20 18 11 CCASF2/JGo M 1 6 062894F "48,250" "±4,564" "48,250" "±4,564" 9.5% "45,646" 105.70 -0.964 ? 940605 940628 23 9.5 384 0 3 agouti (A/-) F2 From Cage 2 case 60 2 necrotic none Dec-20-94 17 "Box 34, Slot R-1" 2 "16,258 x" 247 x RCS RW and RW "2,782 mm^2" "5,418 mm^2" 20.5 µm^2 0.81% 2227 18 390 21.7 ±8.45 ±2.05 2.4524E-03 5.0827E-08 43795 52702 120.3% " 8,907 " 15 23 22 15 10 22 16 14 34 7 18 17 35 30 34 28 29 21 CCASF2/JGo original M 1 6 062894G "77,548" "±3,862" "82,752" "±5,097" 6.2% "78,778" 105.04 2.411 ? 940605 940628 23 9.8 412 0 3 Albino F2 From Cage 2 case 61 1 necrotic none Dec-20-94 21 "Box 34, Slot S-2" 3 "16,258 x" 195 x RCS RW and RW "2,985 mm^2" "5,418 mm^2" 20.5 µm^2 0.83% 3843 32 689 21.5 ±7.38 ±1.33 5.3682E-03 6.9224E-08 70859 94639 133.6% " 23,780 " 12 18 17 12 27 15 22 16 25 13 35 14 27 17 25 26 33 4 26 22 29 28 35 11 18 30 24 25 18 23 21 21 CCASF2/JGo replicate 0.00001 062894G "70,535" "±5,917" 8.4% "77,478" 91.04 Replicate of different thin section. Mar-22-95 21 "Box 34, Slot S-2" "13,010 x" 194 x TK RW and RW "2,915 mm^2" "5,418 mm^2" 32.0 µm^2 0.87% 2420 21 612 29.1 ±10.93 ±2.44 30 27 28 32 19 21 43 20 34 31 19 30 39 43 41 2 18 44 44 22 25 CCASF2/JGo big brain M 1 6 080394X "60,896" "±3,898" "60,896" "±3,898" 6.4% "125,172" 48.65 0.493 ? 940512 940803 83 31.0 547 H 0 4 "agouti (A/-). These are actually F2s, not F1s. Richelle's case number 29. No DNA taken. Mother was a F1 #1, father was F1 case #4. " Brain weighed at 0.5007 gm (547 mg listed in book) none May-12-95 2 "Box 43, Slot G-1" 3 "10,795 x" 192 x RW RW and RW "4,612 mm^2" "5,270 mm^2" 45.2 µm^2 0.83% 2768 23 506 22.0 ±6.61 ±1.41 4.1892E-03 6.8792E-08 17 37 17 18 30 21 26 21 30 29 21 34 19 20 28 16 14 15 18 18 13 19 25 CCASF2/JGo big brain M 1 6 080394Y "77,412" "±5,266" "77,412" "±5,266" 6.8% "133,403" 58.03 2.395 ? 940512 940803 83 29.3 522 H 0 4 "agouti (A/-). These are actually F2s, not F1s. Richelle's case number 30. No DNA taken. Mother was a F1 #1, father was F1 case #4. " Brain weighed at 0.4828 gm (522 mg listed). none May-12-95 4 "Box 43, Slot H-4" 3 "13,021 x" 194 x RCS RW and RW "5,020 mm^2" "5,270 mm^2" 31.1 µm^2 0.61% 4292 26 469 18.0 ±6.14 ±1.23 2.9036E-03 3.7508E-08 27 21 13 19 6 35 19 15 25 17 20 15 17 15 11 16 13 11 23 23 15 21 19 18 10 25 CCASF2/JGo big brain M 1 6 080394Z "52,720" "±4,028" "52,720" "±4,028" 7.6% "60,559" 87.05 -0.449 ? 940512 940803 83 30.0 538 H 0 4 "Albino. These are actually F2s, not F1s. Richelle's case number 31. No DNA taken. Mother was a F1 #1, father was F1 case #4. " Brain weighed at 0.2481 gm but was cut in half. (Listed at .538 gm) none May-12-95 6 "Box 43, Slot I-1" 3 "12,951 x" 247 x RW RW and RW "3,708 mm^2" "5,270 mm^2" 31.4 µm^2 0.88% 1927 17 465 27.4 ±8.36 ±2.09 3.4531E-03 6.5499E-08 31 35 28 24 32 38 29 33 30 15 15 25 23 28 45 18 16 CCASF2/JGo M 1 6 090894E "57,222" "±4,178" "57,222" "±4,178" 7.3% "69,623" 82.19 0.070 ? 940722 940908 48 16.0 458 0 2.2 Albino. Intercross progeny from mating of female F1 #1 or two other females in cage (090894K and L) and male F1 #4. Some confusion re litter. This animal apparent born 7/22/94. "Tissue, not extracted" Dec-5-94 13 "Box 30, Slot D-2" 4 "12,990 x" 193 x RCS RCS and RW "2,603 mm^2" "5,418 mm^2" 32.1 µm^2 1.06% 2168 23 607 26.4 ±9.04 ±1.93 3.4265E-03 5.9881E-08 54203 59985 110.7% " 5,782 " 13 27 35 28 40 28 34 14 30 35 27 33 22 12 40 32 28 8 27 29 12 29 24 CCASF2/JGo original M 1 6 090894F "45,367" "±2,065" "44,920" "±2,737" 6.1% "45,391" 98.96 -1.296 ? 940809 940908 30 14.5 443 0 2.1 Albino. Intercross progeny from mating of female F1 #1 or two other females in cage (090894K and L) and male F1 #4. This animal apparently born 8/9/94. "Tissue, not extracted" Dec-5-94 14 "Box 30, Slot E-1" 3 "12,990 x" 193 x RCS RCS and RW "1,697 mm^2" "5,418 mm^2" 32.1 µm^2 1.27% 1414 18 572 31.8 ±7.98 ±1.94 1.1262E-02 2.4825E-07 41934 47903 114.2% " 5,968 " 38 39 31 15 27 35 21 31 40 34 32 43 35 31 25 35 18 42 CCASF2/JGo replicate 0.00001 090894F "45,958" "±3,148" 6.8% "45,385" 101.26 Replicate by Denny of different grid. Note that the high mag series is shot at a slightly higher than typical value. Mar-29-95 14 "Box 30, Slot E-3" "13,853 x" 245 x DR RW and RW "2,724 mm^2" "5,270 mm^2" 27.5 µm^2 1.27% 1653 21 584 27.8 ±8.52 ±1.90 35 39 25 31 25 26 21 32 10 15 42 36 21 23 22 23 25 38 41 30 24 CCASF2/JGo M 1 6 090894G "65,395" "±2,717" "65,395" "±2,717" 4.2% "77,138" 84.78 1.011 ? 940804 940908 35 18.5 447 H 0 2 agouti (A/-). Intercross progeny from mating of female F1 #1 or two other females in cage (090894K and L) and male F1 #4. Litter of 8.04.94 none "Tissue, not extracted" Dec-5-94 16 "Box 30, Slot G-3" 3 "12,990 x" 193 x RCS RCS and RW "2,884 mm^2" "5,418 mm^2" 32.1 µm^2 1.12% 2402 27 735 27.2 ±5.77 ±1.13 9.2001E-03 1.4069E-07 63143 67815 107.4% " 4,672 " 36 38 25 37 32 28 28 26 28 24 15 24 34 27 22 35 21 26 29 30 34 25 22 25 20 22 22 CCASF2/JGo F 1 6 090894H "50,792" "±4,150" "50,792" "±4,150" 8.2% "63,192" 80.38 -0.671 ? 940804 940908 35 15.0 408 B 1 2 agouti (A/-). Intercross progeny from mating of female F1 #1 or two other females in cage (090894K and L) and male F1 #4. Litter of 8.04.94 Dec-5-94 18 "Box 30, Slot F-2" 4 "12,990 x" 193 x RCS TK and RW "2,363 mm^2" "5,418 mm^2" 32.1 µm^2 1.07% 1968 21 542 25.8 ±9.43 ±2.11 3.0959E-03 6.0952E-08 51755 49913 96.4% " 1,843 " 22 21 40 14 21 17 11 30 25 35 26 13 11 29 22 37 31 41 32 38 26 CCASF2/JGo original F 1 6 090894I "47,227" "±3,621" "51,905" "±6,141" 11.8% "73,786" 70.34 -1.081 ? 940804 940908 35 18.0 420 C 1 2 agouti (A/-). Intercross progeny from mating of female F1 #1 or two other females in cage (090894K and L) and male F1 #4. Litter of 8.04.94 "Tissue, not extracted" Dec-5-94 21 "Box 30, Slot H-2" 4 "12,990 x" 193 x RCS TK and RW "2,759 mm^2" "5,418 mm^2" 32.1 µm^2 0.74% 2298 17 384 22.6 ±10.69 ±2.67 3.8273E-03 8.1041E-08 53615 49977 93.2% " 3,638 " 19 9 16 9 21 15 20 32 23 11 25 27 38 51 20 20 28 CCASF2/JGo replicate 0.00001 090894I "44,733" "±4,483" 10.0% "73,581" 60.79 Replicate analysis by Toya on different thin section 1 necrotic fiber on 034 Mar-22-95 21 "Box 30, Slot H-2" "10,897 x" 192 x TK RW and RW "2,725 mm^2" "5,418 mm^2" 45.6 µm^2 1.18% 1613 19 527 27.7 ±11.79 ±2.78 30 36 40 40 28 35 18 7 5 29 20 28 36 21 27 31 21 55 20 CCASF2/JGo M 1 6 040695M "48,459" "±2,576" "48,459" "±2,576" 5.3% "63,208" 76.67 -0.940 950223 950406 42 20.0 477 H 0 5 Case 189 agouti (A/-) none "Tissue, not extracted" Jun-16-95 14 "Box 47, Slot G-1" 4 "12,923 x" 192 x SS RW and RW "2,326 mm^2" "5,380 mm^2" 32.2 µm^2 1.02% 1962 20 494 24.7 ±5.72 ±1.31 7.6891E-03 1.5867E-07 26 18 19 24 31 31 22 26 33 34 24 25 16 30 25 17 17 32 20 24 CCASF2/JGo F 1 6 040695N "66,567" "±2,962" "66,567" "±2,962" 4.4% "53,077" 125.42 1.146 950223 950406 42 19.6 456 B 1 5 Case 190 agouti (A/-) none "Tissue, not extracted" Jun-16-95 16 "Box 47, Slot F-2" 4 "12,877 x" 192 x SS RW and RW "1,961 mm^2" "5,380 mm^2" 32.4 µm^2 1.41% 1636 23 936 40.7 ±8.49 ±1.81 7.9331E-03 1.1917E-07 54 39 44 41 45 29 42 56 46 29 27 55 31 34 45 38 37 49 37 49 36 31 42 CCASF2/JGo M 1 6 040695O "61,304" "±3,078" "61,304" "±3,078" 5.0% "56,541" 108.43 0.540 950223 950406 42 21.2 444 C 0 5 Case 191 agouti (A/-) none "Tissue, not extracted" Jun-14-95 17 "Box 47, Slot A-1" 3 "12,867 x" 192 x RCS "SS, RCS, and RW" "2,077 mm^2" "5,380 mm^2" 32.5 µm^2 1.21% 1740 21 740 35.2 ±7.91 ±1.77 6.7959E-03 1.1085E-07 33 31 29 33 23 37 35 42 31 47 43 28 48 55 27 36 33 33 38 29 29 CCASF2/JGo M 1 6 040695P "50,451" "±2,725" "50,451" "±2,725" 5.4% "46,712" 108.00 -0.710 950223 950406 42 19.5 411 C 0 5 Case 192 agouti (A/-) "Tissue, not extracted" Jun-8-95 2 "Box 45, Slot I-1" 4 "12,960 x" 247 x RCS RW and RW "2,847 mm^2" "5,380 mm^2" 32.0 µm^2 1.37% 1458 20 692 34.6 ±8.15 ±1.87 7.1518E-03 1.4176E-07 35 48 37 35 37 47 46 34 39 22 15 39 28 36 33 39 25 34 35 28 CCASF2/JGo M 1 6 040695Q "70,988" "±3,288" "70,988" "±3,288" 4.6% "68,472" 103.67 1.655 950223 950406 42 16.4 457 H 0 5 Case 193 agouti (A/-) 1 or 2 necrotic "Tissue, not extracted" Sep-8-95 1 "Box 47, Slot J-2" 4 "12,867 x" 191 x RCS RW and RW "2,509 mm^2" "5,270 mm^2" 31.8 µm^2 1.16% 2151 25 825 33.0 ±7.49 ±1.53 6.8383E-03 9.6331E-08 39 27 35 25 21 27 31 27 23 21 37 35 36 33 26 47 33 38 31 28 45 41 46 39 34 CCASF2/JGo F 1 6 040695R "70,234" "±2,281" "70,234" "±2,281" 3.2% "55,324" 126.95 1.568 950223 950406 42 17.0 448 H 1 5 Case 194 agouti (A/-) none "Tissue, not extracted" Jun-8-95 2 "Box 45, Slot J-2" 4 "12,922 x" 247 x RCS EGW and RW "3,372 mm^2" "5,380 mm^2" 32.2 µm^2 1.22% 1717 21 859 40.9 ±5.94 ±1.33 1.4176E-02 2.0184E-07 36 39 41 35 39 49 37 46 40 39 31 35 35 49 36 43 50 49 37 42 51 CCASF2/JGo M 1 6 040695S "63,626" "±3,203" "63,626" "±3,203" 5.0% "50,170" 126.82 0.807 950223 950406 42 16.5 434 C 0 5 Case 195 albino Perfused by Rob and Richelle. Perfusion rated great. "Tissue, not extracted" Sep-8-95 5 "Box 50, Slot N-1" 4 "12,999 x" 191 x RCS EGW and RW "1,833 mm^2" "5,270 mm^2" 31.2 µm^2 1.24% 1609 20 791 39.6 ±8.68 ±1.99 6.5286E-03 1.0261E-07 43 37 58 39 39 29 34 31 44 33 32 53 50 43 20 39 38 44 40 45 CCASF2/JGo M 1 6 041195F "54,565" "±3,002" "54,565" "±3,002" 5.5% "47,887" 113.95 -0.236 950225 950411 45 16.5 438 C 6 or 7 Case 196 agouti (A/-) "Tissue, not extracted" Jan-10-96 16 "Box 47, Slot H-2" 3 "12,970 x" 190 x RCS RW and RW "1,727 mm^2" "5,263 mm^2" 31.3 µm^2 1.31% 1531 20 713 35.7 ±8.55 ±1.96 6.3742E-03 1.1682E-07 41 36 46 40 17 39 36 33 25 50 26 39 35 46 27 41 24 30 39 43 CCASF2/JGo F 1 6 041195G "47,560" "±3,340" "47,560" "±3,340" 7.0% "61,460" 77.38 -1.043 950225 950411 45 16.3 425 C 1 6 or 7 Case 197 agouti (A/-) none "Tissue, not extracted" Sep-8-95 18 "Box 47, Slot I-1" 3 "12,841 x" 246 x RCS RW and RW "3,722 mm^2" "5,309 mm^2" 32.2 µm^2 1.20% 1909 23 573 24.9 ±8.21 ±1.75 4.4562E-03 9.3697E-08 31 37 9 29 25 7 35 20 18 26 22 32 32 28 26 34 15 27 27 26 33 13 21 CCASF2/JGo runt of litter F 1 6 041195H "40,595" "±3,783" "40,595" "±3,783" 9.3% "47,812" 84.91 -1.845 950225 950411 45 11.5 372 B 1 6 or 7 Case 198. runt of litter. agouti (A/-) none "Tissue, not extracted" Jun-7-95 4 "Box 45, Slot C-1" 4 "12,960 x" 193 x RCS RW and RW "1,774 mm^2" "5,380 mm^2" 32.0 µm^2 1.34% 1492 20 544 27.2 ±11.05 ±2.53 2.9862E-03 7.3561E-08 44 20 20 18 34 18 34 35 37 21 24 28 48 6 28 32 14 43 24 16 CCASF2/JGo M 1 6 041195I "50,763" "±3,202" "50,763" "±3,202" 6.3% "49,320" 102.93 -0.674 950225 950411 45 13.0 414 B 0 6 or 7 Case 199 agouti (A/-) none "Tissue, not extracted" Jun-14-95 1 "Box 47, Slot B-2" 4 "12,915 x" 247 x RCS RW and RW "3,013 mm^2" "5,380 mm^2" 32.3 µm^2 1.31% 1529 20 664 33.2 ±9.13 ±2.09 5.2116E-03 1.0266E-07 45 30 26 30 34 24 30 38 25 28 31 27 40 17 29 31 42 52 52 33 CCASF2/JGo F 1 6 041195J "63,979" "±4,028" "63,979" "±4,028" 6.3% "78,448" 81.56 0.848 950225 950411 45 15.5 437 H 1 6 or 7 Case 200 agouti (A/-) none "Tissue, not extracted" Jun-15-95 3 "Box 47, Slot E-1" 3 "12,626 x" 191 x RCS SS and RCS "2,858 mm^2" "5,380 mm^2" 33.7 µm^2 0.90% 2324 21 578 27.5 ±7.75 ±1.73 4.1400E-03 6.4709E-08 38 28 27 14 20 36 37 35 18 30 16 30 18 33 25 32 26 22 36 37 20 CCASF2/JGo M 1 6 041195K "48,056" "±3,132" "48,056" "±3,132" 6.5% "48,681" 98.72 -0.986 950225 950411 45 15.5 443 H 0 6 or 7 Case 201 agouti (A/-) none "Tissue, not extracted" Jun-14-95 6 "Box 47, Slot C-1" 4 "12,874 x" 248 x RCS RW and RW "2,984 mm^2" "5,380 mm^2" 32.5 µm^2 1.40% 1500 21 673 32.0 ±9.34 ±2.09 5.1441E-03 1.0705E-07 48 44 35 38 36 34 20 32 19 17 28 44 23 22 19 31 38 43 29 33 40 CCASF2/JGo M 1 6 041195L "68,041" "±4,059" "68,041" "±4,059" 6.0% "58,597" 116.12 1.316 950225 950411 45 17.8 458 B 0 6 or 7 Case 202 agouti (A/-) none "Tissue, not extracted" Jun-8-95 4 "Box 45, Slot D" 4 "12,873 x" 248 x RCS RW and RW "3,603 mm^2" "5,380 mm^2" 32.5 µm^2 1.11% 1805 20 754 37.7 ±9.80 ±2.25 4.3466E-03 6.3882E-08 32 27 37 20 41 34 51 47 40 44 30 48 54 32 39 51 26 40 39 22 CCASF2/JGo F 1 6 041195M "64,723" "±3,208" "64,723" "±3,208" 5.0% "59,894" 108.06 0.934 950225 950411 45 15.5 400 H 1 6 or 7 Case 203 agouti (A/-) 1 necrotic "Tissue, not extracted" Sep-8-95 10 "Box 47, Slot K-4" 4 "13,017 x" 245 x RCS EGW and RW "3,603 mm^2" "5,270 mm^2" 31.1 µm^2 0.93% 1926 18 605 33.6 ±6.87 ±1.67 6.6574E-03 1.0286E-07 42 35 39 33 32 38 37 26 33 34 36 28 19 41 25 39 44 24 CCASF2/JGo F 1 6 041195N "60,421" "±4,083" "60,421" "±4,083" 6.8% "50,216" 120.32 0.438 950225 950411 45 15.3 444 H 1 6 or 7 Case 204 albino none "Tissue, not extracted" Jun-6-95 ? "Box 45, Slot A-1" 4 "12,884 x" 242 x RCS RW and RW "2,942 mm^2" "5,380 mm^2" 32.4 µm^2 1.23% 1549 19 741 39.0 ±11.18 ±2.64 3.8263E-03 6.3328E-08 25 37 39 45 45 41 36 44 16 40 19 38 51 50 45 56 34 26 54 CCASF2/JGo M 1 6 041195O "52,738" "±3,464" "52,738" "±3,464" 6.6% "46,433" 113.58 -0.447 950225 950411 45 16.3 424 C 0 6 or 7 Case 205 albino none "Tissue, not extracted" Jun-7-95 ? "Box 45, Slot B-2" 4 "12,935 x" 248 x RCS RW and RW "2,847 mm^2" "5,380 mm^2" 32.2 µm^2 1.32% 1444 19 694 36.5 ±10.18 ±2.40 4.6397E-03 8.7977E-08 50 38 32 29 22 38 38 31 26 20 56 20 45 38 37 49 39 42 44 CCASF2/JGo F 1 6 051295A "49,893" "±2,416" "49,893" "±2,416" 4.8% "42,137" 118.41 -0.774 950403 950512 39 12.8 407 B 1 8 Case 216 agouti (A/-) light coffee none "Tissue, not extracted" F1 #60 #34 Sep-19-95 4 "Box 54, Slot K-2" 3 "12,890 x" 192 x RCS RCS and RW "1,555 mm^2" "5,360 mm^2" 32.3 µm^2 1.53% 1306 20 764 38.2 ±8.06 ±1.85 8.9977E-03 1.8034E-07 43 60 38 38 38 52 35 39 36 42 25 39 31 45 34 25 36 40 32 36 CCASF2/JGo R or L M 1 6 051295B "58,257" "±3,770" "53,409" "±5,047" 9.5% "71,255" 74.96 0.189 R or L 950403 950512 39 18.1 447 H 0 8 agouti (A/-) light coffee none "Tissue, not extracted" F1 #60 #34 Jun-29-95 5 "Box 49, Slot B-2" 3 "12,902 x" 193 x SS RCS and RCS "2,655 mm^2" "5,380 mm^2" 32.3 µm^2 1.00% 2205 22 533 24.2 ±10.49 ±2.29 4.2937E-03 7.3702E-08 16 15 9 9 14 14 20 27 22 27 17 31 31 51 36 19 30 18 23 37 36 31 CCASF2/JGo L or R 0.00001 051295B "64,374" "±5,670" 8.8% "72,014" 89.39 L of R Other side of this case none Jun-30-95 6 "Box 49, Slot F-4" 3 "12,909 x" 193 x SS RCS and RCS "2,683 mm^2" "5,380 mm^2" 32.3 µm^2 0.99% 2230 22 635 28.9 ±11.65 ±2.54 30 39 33 30 39 21 27 19 5 18 27 55 46 44 18 25 42 28 16 22 29 22 CCASF2/JGo F 1 6 051295C "60,678" "±4,164" "60,678" "±4,164" 6.9% "61,164" 99.21 0.468 950403 950512 39 14.5 442 H 1 8 agouti (A/-) none none F1 #60 #34 Jun-30-95 8 "Box 49, Slot D-4" 4 "12,934 x" 248 x SS RCS and RCS "3,748 mm^2" "5,380 mm^2" 32.2 µm^2 1.10% 1902 21 670 31.9 ±9.79 ±2.19 3.6739E-03 6.0547E-08 32 14 32 32 37 44 42 31 25 24 23 48 38 14 36 42 21 20 38 36 41 CCASF2/JGo M 051295D 950403 950512 39 18.5 441 8 agouti (A/-) "Tissue, not extracted" F1 #60 #34 RCS and RCS "3,748 mm^2" "5,380 mm^2" #DIV/0! #DIV/0! #DIV/0! 0 0 #DIV/0! #DIV/0! #DIV/0! CCASF2/JGo F 1 6 051295E "47,135" "±3,638" "47,135" "±3,638" 7.7% "67,847" 69.47 -1.092 950403 950512 39 16.5 423 C 1 8 agouti (A/-) none "Tissue, not extracted" F1 #60 #34 Jun-30-95 11 "Box 49, Slot G-3" 4 "12,915 x" 193 x SS SS and SS "2,518 mm^2" "5,380 mm^2" 32.3 µm^2 1.05% 2103 22 493 22.4 ±7.93 ±1.73 3.7308E-03 7.9150E-08 33 22 21 16 18 32 22 13 19 2 33 25 37 24 31 22 16 21 18 18 29 21 CCASF2/JGo poor fixation M 051295F 950403 950512 39 14.0 424 8 albino Poor fixation "Tissue, not extracted" F1 #60 #34 Jun-29-95 13 "Box 49, Slot C-1" 4 "12,934 x" 248 x SS RCS and RCS "3,748 mm^2" "5,380 mm^2" 32.2 µm^2 #DIV/0! 0 0 0 #DIV/0! #DIV/0! #DIV/0! CCASF2/JGo M 1 6 051295G "46,551" "±3,047" "46,551" "±3,047" 6.5% "48,714" 95.56 -1.159 950403 950512 39 15.3 414 C 0 8 albino none "Tissue, not extracted" F1 #60 #34 Jun-29-95 15 "Box 49, Slot A-1" 3 "12,918 x" 248 x SS RCS and RCS "2,991 mm^2" "5,380 mm^2" 32.2 µm^2 1.39% 1511 21 647 30.8 ±9.02 ±2.02 5.2631E-03 1.1306E-07 24 26 28 32 21 47 32 41 27 43 27 18 34 32 35 21 37 14 42 24 42 CCASF2/JGo F 051295H 950403 950512 39 17.0 471 8 albino "Tissue, not extracted" F1 #60 #34 RCS and RCS "3,748 mm^2" "5,380 mm^2" #DIV/0! #DIV/0! #DIV/0! 0 0 #DIV/0! #DIV/0! #DIV/0! CCASF2/JGo F 1 6 051295I "62,632" "±2,641" "62,632" "±2,641" 4.2% "70,145" 89.29 0.693 950403 950512 39 17.5 452 H 1 8 albino none "Tissue, not extracted" F1 #60 #34 Jun-30-95 20 "Box 49, Slot E-3" 3 "12,871 x" 193 x SS RCS and RCS "2,606 mm^2" "5,380 mm^2" 32.5 µm^2 0.97% 2160 21 609 29.0 ±5.47 ±1.22 9.4308E-03 1.5058E-07 36 25 27 25 31 33 34 21 21 25 25 22 35 29 34 34 38 26 22 32 34 CCASF2/JGo F 051295J 950403 950512 39 12.5 418 8 Case 227 agouti (A/-). "Tissue, not extracted" F1 #60 #34 "3,748 mm^2" "5,380 mm^2" #DIV/0! #DIV/0! #DIV/0! 0 0 #DIV/0! #DIV/0! #DIV/0! CCASF2/JGo extreme runt F 061495A "42,513" "±2,469" "42,513" "±2,469" 5.8% "30,711" 138.43 -1.624 950507 950614 38 see notes see notes C 9 or 10 Case 239. Runt with a 323 mg brain. Body weight only 9 gm. Two litters in same box. Cases 239 to 247 either born 5/2/95 or 5/12/95 albino "Tissue, not extracted" #59 or 35 #34 Dec-8-95 18 "Box 52, Slot M-2" 3 "12,806 x" 246 x RCS RCS and RW "1,862 mm^2" "5,225 mm^2" 31.9 µm^2 2.07% 964 20 882 44.1 ±11.16 ±2.56 33 42 38 28 37 22 32 55 44 50 51 28 46 45 54 51 61 54 57 54 CCASF2/JGo F 1 6 061495B "68,133" "±6,170" "68,133" "±6,170" 9.1% "92,082" 73.99 1.326 950507 950614 38 15.0 460 B 1 9 or 10 Case 240 agouti (A/-) none "Tissue, not extracted" #59 or 35 #34 Dec-8-95 20 "Box 52, Slot N-1" 3 "12,936 x" 192 x RCS RCS and RW "3,393 mm^2" "5,225 mm^2" 31.2 µm^2 0.68% 2949 20 462 23.1 ±9.12 ±2.09 33 13 6 31 25 33 28 11 18 6 26 26 25 27 35 29 15 17 34 24 CCASF2/JGo F 1 6 061495C "45,121" "±1,976" "45,121" "±1,976" 4.4% "42,095" 107.19 -1.324 950507 950614 38 12.0 344 C 1 9 or 10 Case 242 agouti (A/-) maybe 1 necrotic "Tissue, not extracted" #59 or 35 #34 Dec-8-95 1 "Box 52, Slot O-2" 4 "12,937 x" 192 x RCS RCS and RW "1,545 mm^2" "5,225 mm^2" 31.2 µm^2 1.93% 1348 26 870 33.5 ±7.33 ±1.47 38 30 38 29 28 31 27 45 18 23 42 41 43 31 30 30 40 30 26 41 24 38 46 34 37 30 CCASF2/JGo F 1 6 061495D "49,114" "±3,961" "49,114" "±3,961" 8.1% "58,292" 84.25 -0.864 ? 950507 950614 38 11.0 391 C 1 9 or 10 Case 243 agouti (A/-) none "Tissue, not extracted" #59 or 35 #34 Dec-8-95 2 "Box 52, Slot P-1" 4 "13,036 x" 192 x RCS RCS and RW "2,139 mm^2" "5,225 mm^2" 30.7 µm^2 1.11% 1896 21 544 25.9 ±9.34 ±2.09 39 30 37 13 34 30 21 9 22 32 15 31 17 40 18 20 19 17 32 32 36 39 30 37 13 34 30 21 CCASF2/JGo F 061495E 950507 950614 38 14.3 381 9 or 10 Case 244 agouti (A/-) "Tissue, not extracted" #59 or 35 #34 CCASF2/JGo poor fixation F 061495F poor fixation ? 950507 950614 38 15.8 456 1 9 or 10 Case 245 agouti (A/-) light coffee "Tissue, not extracted" #59 or 35 #34 Dec-7-96 6 "Box 52, Slot R-1" 2 "12,999 x" 191 x RCS RCS and RW "1,837 mm^2" "5,263 mm^2" 31.1 µm^2 #DIV/0! 0 0 0 #DIV/0! #DIV/0! #DIV/0! #VALUE! #DIV/0! CCASF2/JGo F 1 6 061495G "53,492" "±4,027" "53,492" "±4,027" 7.5% "92,520" 57.82 -0.360 ? 950507 950614 38 15.5 419 1 9 or 10 Case 246 agouti (A/-) light coffee none "Tissue, not extracted" #59 or 35 #34 Jan-7-96 8 "Box 52, Slot S-2" 4 "12,879 x" 191 x RCS RCS and RW "3,361 mm^2" "5,263 mm^2" 31.7 µm^2 0.79% 2915 23 422 18.3 ±6.48 ±1.38 3.4492E-03 6.4481E-08 25 11 5 25 16 13 16 31 17 24 11 23 25 22 14 11 24 10 19 14 21 23 22 CCASF2/JGo F 061495H 950507 950614 38 13.5 392 9 or 10 Case 247 agouti (A/-) light coffee "Tissue, not extracted" #59 or 35 #34 CCASF2/JGo F 1 6 071095A "49,531" "±3,180" "49,531" "±3,180" 6.4% "49,539" 99.98 -0.816 950604 950710 36 13.0 413 1 12 Case 273 albino #36 #34 Dec-15-95 18 "Box 52, Slot B-2" 3 "12,967 x" 244 x RCS RCS and RW "2,957 mm^2" "5,263 mm^2" 31.3 µm^2 1.26% 1582 20 626 31.3 ±8.76 ±2.01 5.1544E-03 1.0406E-07 31 25 32 22 33 45 34 34 13 28 33 13 29 27 37 43 38 44 37 28 CCASF2/JGo F 1 6 071095B "53,948" "±3,817" "53,948" "±3,817" 7.1% "58,782" 91.78 -0.307 ? 950604 950710 36 12.0 401 1 12 Case 274 agouti (A/-) none #36 #34 Oct-8-95 21 "Box 52, Slot C-1" 3 "13,029 x" 190 x RCS RCS and RW "2,127 mm^2" "5,263 mm^2" 31.0 µm^2 1.16% 1896 22 626 28.5 ±9.23 ±2.01 3.8792E-03 7.1906E-08 32 8 30 17 34 42 28 28 30 37 18 29 23 35 35 22 18 15 46 32 35 32 CCASF2/JGo F 1 6 071095C "56,194" "±4,978" "56,194" "±4,978" 8.9% "56,489" 99.48 -0.049 ? 950604 950710 36 16.0 427 1 12 Case 275 agouti (A/-) 2 necrotic #36 #34 Dec-15-95 1 "Box 52, Slot D-2" 2 "12,893 x" 245 x RCS RCS and RW "3,391 mm^2" "5,263 mm^2" 31.7 µm^2 1.12% 1784 20 630 31.5 ±12.16 ±2.79 2.3870E-03 4.2477E-08 40 20 31 44 43 45 47 30 30 41 18 31 45 12 42 32 6 19 34 20 CCASF2/JGo M 1 6 071095D "56,569" "±5,023" "56,569" "±5,023" 8.9% "48,394" 116.89 -0.006 ? 950604 950710 36 15.8 388 0 12 Case 276 light coffee agouti none #36 #34 Oct-8-95 3 "Box 52, Slot E-1" 3 "12,963 x" 191 x RCS RCS and RW "1,768 mm^2" "5,263 mm^2" 31.3 µm^2 1.16% 1545 18 659 36.6 ±13.40 ±3.25 2.3739E-03 4.1965E-08 49 42 51 50 45 61 47 43 31 19 41 29 38 21 11 24 34 23 CCASF2/JGo M 1 6 071095E "60,553" "±3,369" "60,553" "±3,369" 5.6% "46,326" 130.71 0.453 ? 950604 950710 36 15.5 402 0 12 Case 277 albino none #36 #34 Dec-15-95 6 "Box 52, Slot F-2" 3 "12,938 x" 244 x RCS RCS and RW "2,762 mm^2" "5,263 mm^2" 31.4 µm^2 1.36% 1473 20 822 41.1 ±9.97 ±2.29 5.6153E-03 9.2734E-08 47 59 44 28 12 47 50 48 41 40 36 54 36 39 37 39 33 44 42 46 CCASF2/JGo F 1 6 071095F "59,605" "±3,539" "59,605" "±3,539" 5.9% "54,663" 109.04 0.344 ? 950604 950710 36 11.3 392 1 12 Case 278 agouti (A/-) none #36 #34 Dec-15-95 7 "Box 52, Slot G-1" 2 "13,021 x" 191 x RCS RCS and RW "1,992 mm^2" "5,263 mm^2" 31.0 µm^2 1.14% 1761 20 677 33.9 ±8.76 ±2.01 5.0083E-03 8.4025E-08 31 28 31 58 45 41 38 16 27 31 37 31 32 36 41 21 34 34 35 30 CCASF2/JGo F 071095G -6.520 ? 950604 950710 36 14.0 420 12 Case 279 agouti (A/-) #36 #34 CCASF2/JGo M 1 6 071095H "71,030" "±5,066" "71,030" "±5,066" 7.1% "64,299" 110.47 1.660 ? 950604 950710 36 15.5 423 0 12 Case 280 agouti (A/-) none #36 #34 Dec-20-95 11 "Box 52, Slot I-1" 3 "12,958 x" 190 x RCS RCS and RW "2,333 mm^2" "5,263 mm^2" 31.3 µm^2 1.17% 2051 24 831 34.6 ±11.84 ±2.47 2.8884E-03 4.0665E-08 38 33 28 43 48 65 40 36 32 41 17 25 48 46 39 28 30 47 31 16 23 18 38 21 CCASF2/JGo F 1 6 071095I "50,969" "±2,702" "50,969" "±2,702" 5.3% "44,867" 113.60 -0.651 ? 950604 950710 36 12.5 373 1 12 Case 281 agouti (A/-) 1 necrotic #36 #34 Dec-20-95 14 "Box 52, Slot J-2" 3 "12,970 x" 191 x RCS RCS and RW "1,639 mm^2" "5,263 mm^2" 31.3 µm^2 1.67% 1434 24 853 35.5 ±9.04 ±1.88 7.2849E-03 1.4293E-07 41 31 29 30 36 36 20 23 30 53 39 27 36 35 48 36 51 40 22 35 34 40 52 29 CCASF2/JGo M 1 6 071095J "58,095" "±3,633" "58,095" "±3,633" 6.3% "57,397" 101.22 0.170 ? 950604 950710 36 14.0 393 0 12 Case 282 agouti (A/-) none #36 #34 Jan-7-96 16 "Box 52, Slot K-2" 3 "12,816 x" 190 x RCS RW and RW "2,073 mm^2" "5,263 mm^2" 32.0 µm^2 1.28% 1791 23 746 32.4 ±9.51 ±2.03 4.6007E-03 7.9192E-08 29 32 28 35 44 33 49 44 38 15 24 41 34 21 33 26 39 19 18 46 37 38 23 CCASF2/JGo F 1 6 071095K "57,319" "±2,733" "57,319" "±2,733" 4.8% "65,889" 86.99 0.081 ? 950604 950710 36 15.0 428 1 12 Case 283 agouti (A/-) none #36 #34 Oct-8-95 18 "Box 52, Slot L-1" 3 "13,155 x" 191 x RCS RCS and RW "2,407 mm^2" "5,263 mm^2" 30.4 µm^2 1.11% 2166 24 635 26.5 ±6.05 ±1.26 8.0071E-03 1.3969E-07 32 20 25 24 25 39 19 18 30 31 26 24 26 41 27 21 29 34 31 24 24 20 19 26 CCASF2/JGo F 1 6 072195F "51,879" "±3,428" "51,879" "±3,428" 6.6% "62,979" 82.37 -0.546 ? 950614 950721 37 14.0 404 11 Case 287 albino #59 #34 Jan-10-96 18 "Box 56, Slot L-1" 4 "13,016 x" 191 x RW RW and RW "2,304 mm^2" "5,263 mm^2" 31.1 µm^2 1.09% 2027 22 563 25.6 ±7.75 ±1.69 4.6238E-03 8.9125E-08 31 31 27 32 23 26 42 25 25 23 37 21 17 4 22 26 28 20 31 31 24 17 CCASF2/JGo F 1 6 072195G "58,698" "±3,804" "58,698" "±3,804" 6.5% "72,295" 81.19 0.240 ? 950614 950721 37 13.0 409 1 11 Case 288 agouti (A/-) none #59 #34 Jan-9-96 17 "Box 56, Slot K-1" 4 "13,012 x" 190 x RCS RW and RW "2,619 mm^2" "5,263 mm^2" 31.1 µm^2 0.90% 2326 21 530 25.2 ±7.31 ±1.64 4.2602E-03 7.2578E-08 33 30 23 23 26 21 39 29 31 17 9 23 25 36 19 29 14 29 30 18 26 CCASF2/JGo F 1 6 072195H "51,287" "±1,841" "51,287" "±1,841" 3.6% "48,845" 105.00 -0.614 ? 950614 950721 37 12.5 403 1 11 Case 289 agouti (A/-) none #59 #34 Jan-9-96 16 "Box 56, Slot J-2" 3 "13,112 x" 191 x RCS RW and RW "1,787 mm^2" "5,263 mm^2" 30.6 µm^2 1.32% 1596 21 675 32.1 ±5.16 ±1.15 1.5887E-02 3.0977E-07 34 29 28 34 24 42 38 24 40 33 33 36 35 23 28 37 35 30 29 31 32 CCASF2/JGo F 1 6 072195I "58,263" "±3,854" "58,263" "±3,854" 6.6% "61,900" 94.12 0.190 ? 950614 950721 37 12.5 400 1 11 Case 290 agouti (A/-) none #59 #34 Jan-7-96 15 "Box 56, Slot I-1" 4 "12,959 x" 190 x RCS RW and RW "2,242 mm^2" "5,263 mm^2" 31.3 µm^2 1.11% 1975 22 649 29.5 ±8.94 ±1.95 4.1088E-03 7.0522E-08 36 25 23 28 36 6 31 15 16 26 26 28 31 41 34 29 31 36 39 40 42 30 CCASF2/JGo F 1 6 072195J "47,485" "±3,134" "47,485" "±3,134" 6.6% "58,675" 80.93 -1.052 ? 950614 950721 37 10.3 345 1 11 Case 291 agouti (A/-) none #59 #34 Dec-11-95 14 "Box 56, Slot H-2" 4 "13,052 x" 192 x RCS RCS "2,156 mm^2" "5,225 mm^2" 30.7 µm^2 1.15% 1913 22 546 24.8 ±7.51 ±1.64 5.0646E-03 1.0666E-07 15 24 31 39 19 17 17 10 20 38 25 34 30 27 28 24 20 31 23 22 20 32 CCASF2/JGo F 1 6 072195K "48,251" "±2,511" "48,251" "±2,511" 5.2% "55,587" 86.80 -0.964 ? 950614 950721 37 12.5 403 1 11 Case 292 agouti (A/-) none #59 #34 Dec-11-95 13 "Box 56, Slot G-1" 4 "12,996 x" 193 x RCS RCS and RW "2,080 mm^2" "5,225 mm^2" 30.9 µm^2 1.11% 1797 20 537 26.9 ±6.09 ±1.40 8.0586E-03 1.6702E-07 18 24 31 24 10 30 25 31 31 27 19 33 34 27 27 33 24 34 25 30 CCASF2/JGo F 1 6 072195L "47,169" "±2,708" "47,169" "±2,708" 5.7% "56,507" 83.47 -1.088 ? 950614 950721 37 12.3 382 1 11 Case 293 "agouti, light coffee (A/-)" 1 necrotic #59 #34 Dec-7-95 12 "Box 56, Slot F-2" 4 "13,012 x" 192 x RCS RCS and RW "2,076 mm^2" "5,263 mm^2" 31.1 µm^2 1.05% 1818 19 493 25.9 ±6.32 ±1.49 6.7898E-03 1.4395E-07 29 29 19 32 17 26 29 12 24 23 35 27 24 21 22 32 25 38 29 CCASF2/JGo F 1 6 072195M "52,263" "±3,533" "52,263" "±3,533" 6.8% "60,705" 86.09 -0.501 ? 950614 950721 37 13.5 416 1 11 Case 294 "agouti, light coffee (A/-)" none #59 #34 Dec-7-95 11 "Box 56, Slot E-1" 4 "12,997 x" 192 x RCS RCS and RW "2,227 mm^2" "5,263 mm^2" 31.2 µm^2 1.18% 1948 23 617 26.8 ±8.50 ±1.81 4.3784E-03 8.3776E-08 21 26 30 36 45 25 33 33 26 29 29 27 21 40 32 12 18 16 14 15 25 36 28 CCASF2/JGo M 1 6 072195N "70,165" "±4,363" "70,165" "±4,363" 6.2% "76,052" 92.26 1.560 ? 950614 950721 37 12.3 397 0 11 Case 295 "agouti, light coffee (A/-)" 2 necrotic #59 #34 Dec-7-95 10 "Box 56, Slot D-2" 4 "13,032 x" 192 x RCS RCS and RW "2,802 mm^2" "5,263 mm^2" 31.0 µm^2 1.10% 2454 27 772 28.6 ±9.06 ±1.78 3.8284E-03 5.4564E-08 36 21 36 28 25 21 20 33 28 23 34 51 28 31 10 5 28 34 34 31 23 25 37 40 33 30 27 CCASF2/JGo F 1 6 072195O "56,069" "±4,876" "56,069" "±4,876" 8.7% "69,737" 80.40 -0.063 ? 950614 950721 37 15.0 441 1 11 Case 296 "agouti, light coffee (A/-)" none #59 #34 Dec-7-95 9 "Box 56, Slot C1" 4 "12,844 x" 193 x RCS RCS and RW "2,591 mm^2" "5,263 mm^2" 31.9 µm^2 0.91% 2186 20 513 25.7 ±9.72 ±2.23 2.4820E-03 4.4266E-08 18 16 20 40 35 32 37 12 15 15 22 37 30 39 31 38 21 17 23 15 CCASF2/JGo get block number M 1 6 081595A "58,622" "±4,120" "58,622" "±4,120" 7.0% "78,739" 74.45 0.231 ? 950702 950815 44 19.0 444 0 Case 310 agouti (A/-) 1 necrotic 35 or 36 #34 Jan-25-96 "Box 59, Slot A-1" 4 "12,907 x" 191 x RCS RW and RW "2,881 mm^2" "5,263 mm^2" 31.6 µm^2 0.84% 2492 21 494 23.5 ±7.39 ±1.65 3.6265E-03 6.1863E-08 32 21 5 20 22 15 23 28 40 36 24 23 31 22 19 23 21 20 18 25 26 CCASF2/JGo get block number M 1 6 081595B "57,523" "±3,513" "57,523" "±3,513" 6.1% "64,495" 89.19 0.104 ? 950702 950815 44 21.0 430 0 Case 308 agouti (A/-) none 35 or 36 #34 Jan-25-96 "Box 59, Slot B-2" 4 "12,985 x" 192 x RCS RW and RW "2,376 mm^2" "5,263 mm^2" 31.2 µm^2 0.92% 2066 19 529 27.8 ±7.21 ±1.70 4.9211E-03 8.5550E-08 36 35 30 32 28 23 34 33 27 27 31 10 31 27 30 35 20 12 28 CCASF2/JGo F 1 6 081595C "59,843" "±3,158" "59,843" "±3,158" 5.3% "90,847" 65.87 0.372 ? 950702 950815 44 12.5 397 1 Case 314 agouti (A/-) none 35 or 36 #34 Jan-28-96 3 "Box 59, Slot C-1" 4 "12,997 x" 192 x RCS RW and RW "3,343 mm^2" "5,263 mm^2" 31.2 µm^2 0.72% 2916 21 431 20.5 ±4.84 ±1.08 6.3002E-03 1.0528E-07 25 20 21 23 16 33 23 24 16 28 14 18 16 17 25 20 19 15 17 24 17 CCASF2/JGo F 1 6 081595D "58,164" "±2,555" "58,164" "±2,555" 4.4% "74,117" 78.48 0.178 ? 950702 950815 44 17.0 432 1 Case 305 agouti (A/-) none 35 or 36 #34 Jan-28-96 4 "Box 59, Slot D-2" 4 "13,021 x" 192 x RCS RW and RW "2,727 mm^2" "5,263 mm^2" 31.0 µm^2 0.92% 2387 22 536 24.4 ±4.90 ±1.07 9.3347E-03 1.6049E-07 21 31 21 18 32 21 29 22 28 26 29 18 28 28 21 20 32 21 22 20 30 18 CCASF2/JGo F 1 6 081595E "54,634" "±3,313" "54,634" "±3,313" 6.1% "76,061" 71.83 -0.228 ? 950702 950815 44 15.5 426 1 Case 312 agouti (A/-) none 35 or 36 #34 Jan-28-96 5 "Box 59, Slot E-1" 4 "12,923 x" 192 x RCS RW and RW "2,799 mm^2" "5,263 mm^2" 31.5 µm^2 0.91% 2414 22 498 22.6 ±6.29 ±1.37 5.2137E-03 9.5431E-08 19 13 24 14 27 26 30 29 21 20 28 27 26 16 24 13 26 37 18 18 26 16 CCASF2/JGo poor fix F 081595F ? 950702 950815 44 14.5 443 1 Case 306. Poor fixation. Unmyelinated fibers not visible. agouti (A/-) 35 or 36 #34 Jan-28-96 6 "Box 59, Slot F-2" 1 "12,953 x" 191 x RCS RW and RW "2,703 mm^2" "5,263 mm^2" 31.4 µm^2 #DIV/0! 0 0 0 #DIV/0! #DIV/0! #DIV/0! #DIV/0! #DIV/0! CCASF2/JGo M 1 6 081595G "62,271" "±5,086" "62,271" "±5,086" 8.2% "80,091" 77.75 0.651 ? 950702 950815 44 19.5 450 0 Case 311 agouti (A/-) none 35 or 36 #34 Jan-28-96 7 "Box 59, Slot G-1" 4 "12,929 x" 192 x RCS RW and RW "2,939 mm^2" "5,263 mm^2" 31.5 µm^2 0.98% 2544 25 612 24.5 ±9.79 ±2.00 2.5081E-03 4.0276E-08 10 28 32 23 34 23 7 31 10 45 29 41 32 31 18 21 13 26 12 20 26 33 26 27 14 CCASF2/JGo M 1 6 081595H "33,515" "±1,926" "33,515" "±1,926" 5.7% "36,284" 92.37 -2.661 ? 950702 950815 44 15.3 434 0 Case 315 agouti (A/-) perhaps 1 35 or 36 #34 Jan-28-96 8 "Box 59, Slot H-2" 3 "13,046 x" 245 x RCS RW and RW "2,171 mm^2" "5,263 mm^2" 30.9 µm^2 1.96% 1173 23 657 28.6 ±7.70 ±1.64 9.4498E-03 2.8195E-07 33 32 25 41 33 38 36 33 17 37 23 29 31 32 28 31 8 17 32 27 23 21 30 CCASF2/JGo F 081595I 950702 950815 44 16.3 421 Case 313 agouti (A/-) 35 or 36 #34 CCASF2/JGo M 081595J 950702 950815 44 17.0 393 Case 304 light coffee agouti 35 or 36 #34 CCASF2/JGo F 081595K 950702 950815 44 14.0 455 Case 316 light coffee agouti 35 or 36 #34 CCASF2/JGo M 081595L 950702 950815 44 18.5 417 Case 309 light coffee agouti 35 or 36 #34 CCASF2/JGo F 081595M 950702 950815 44 14.0 407 Case 303 albino 35 or 36 #34 CCASF2/JGo F 081595N 950702 950815 44 15.5 447 Case 307 albino 35 or 36 #34 CCASF2/JGo F 081595O 950702 950815 44 8.5 371 Case 317 albino 35 or 36 #34 CCASF2/JGo M 081595P 950702 950815 44 21.5 462 Case 335 agouti (A/-) 35 or 36 #34 CCASF2/JGo F 081595Q 950702 950815 44 13.5 424 Case 331 agouti (A/-) 35 or 36 #34 CCASF2/JGo F 081595R 950702 950815 44 18.5 487 Case 328 agouti (A/-) 35 or 36 #34 CCASF2/JGo M 081595S 950702 950815 44 15.0 421 Case 332 agouti (A/-) 35 or 36 #34 CCASF2/JGo M 081595T 950702 950815 44 18.0 459 Case 333 agouti (A/-) 35 or 36 #34 CCASF2/JGo F 081595U 950702 950815 44 15.0 421 Case 336 agouti (A/-) 35 or 36 #34 CCASF2/JGo F 081595V 950702 950815 44 17.0 491 Case 329 albino 35 or 36 #34 CCASF2/JGo F 081595W 950702 950815 44 17.5 465 Case 327 albino 35 or 36 #34 CCASF2/JGo M 081595X 950702 950815 44 19.0 461 Case 330 albino 35 or 36 #34 CCASF2/JGo F 101295I 950906 951012 36 15.0 421 Case 367 albino #59 #34 CCASF2/JGo M 101295J 950906 951012 36 16.0 404 Case 368 albino #59 #34 CCASF2/JGo F 101295K 950906 951012 36 15.0 421 Case 369 Agouti #59 #34 CCASF2/JGo F 101295L 950906 951012 36 17.0 411 Case 370 Agouti #59 #34 CCASF2/JGo F 101295M 950906 951012 36 15.0 420 Case 371 Agouti #59 #34 CCASF2/JGo F 101295N 950906 951012 36 14.5 414 Case 372 Agouti #59 #34 CCASF2/JGo M 101295O 950906 951012 36 15.5 413 Case 373 Agouti #59 #34 CCASF2/JGo F 101295P 950906 951012 36 12.5 389 Case 374 Agouti #59 #34 CCASF2/JGo M 101295R 950906 951012 36 14.8 391 Case 376 Light coffee agouti #59 #34 CCASF2/JGo F 091895K 950725 950918 55 20.0 489 Case 348 Light coffee agouti #35 #34 CCASF2/JGo M 091895L 950725 950918 55 18.8 434 Case 347 Light coffee agouti #35 #34 CCASF2/JGo M 091895M 950725 950918 55 19.1 417 Case 349 Agouti #35 #34 CCASF2/JGo M 091895N 950813 950918 36 20.8 455 Case 354 Agouti #35 #34 CCASF2/JGo F 091895O 950813 950918 36 18.5 448 Case 355 Agouti #35 #34 CCASF2/JGo M 091895P 950813 950918 36 17.0 452 Case 356 Agouti #35 #34 CCASF2/JGo GET 091895Q 950813 950918 36 12.5 383 Case 357 #35 #34 CCASCN2 M 1 8 090894A "60,679" "±1,571" "±3,847" 7 "62,174" "±6,910" "62,174" "±6,910" 11.1% "80,441" 77.29 0.388 108 ? 472 940804 940908 35 22.0 483 0 1 Backcross progeny (BALB/c x CAST/Ei) x BALB/c. Agouti "From Cage 4, Case 108. Hard to count unmyelinated fibers in some photos, due to oblique angle." "Tissue, not extracted" Dec-4-94 4 "Box 20, Slot G-1" 4 "13,055 x" 193 x TK RW and RW "2,996 mm^2" "5,418 mm^2" 31.8 µm^2 0.83% 2530 21 516 24.6 ±12.21 ±2.73 1.3672E-03 2.1990E-08 64866 59208 91.3% " 5,659 " 28 28 32 33 43 17 43 33 30 15 0 13 21 27 39 39 13 5 10 24 23 CCASCN2 F 1 8 090894B "57,849" "±4,032" "57,849" "±4,032" 7.0% "71,146" 81.31 -0.736 109 ? 940804 940908 35 18.3 512 1 1 Backcross progeny (BALB/c x CAST/Ei) x BALB/c. Agouti "Cage 4, case 109" "Tissue, not extracted" Dec-7-94 6 "Box 30, Slot N-1" 4 "12,962 x" 193 x TK RCS and RW "2,650 mm^2" "5,418 mm^2" 32.2 µm^2 0.82% 2206 18 472 26.2 ±7.53 ±1.83 3.7687E-03 6.5146E-08 56377 59318 105.2% " 2,941 " 28 24 15 30 26 20 not in nerve 32 18 20 23 28 28 39 27 16 42 21 35 CCASCN2 original M 1 8 090894C "53,093" "±2,015" "47,088" "±4,899" 10.4% "53,355" 88.26 -1.972 110 ? 940804 940908 35 21.8 512 0 1 Backcross progeny (BALB/c x CAST/Ei) x BALB/c. Agouti. No problem with tissue or ID of animal type; just a small population. "Cage 4, case 110" "Tissue, not extracted" Dec-7-94 8 "Box 33, Slot Q-2" 2 "13,068 x" 193 x RCS RCS and RW "1,987 mm^2" "5,418 mm^2" 31.7 µm^2 0.77% 1682 13 364 28.0 ±10.09 ±2.91 1.4167E-02 2.6683E-07 51291 43483 84.8% " 7,808 " 21 24 44 39 26 38 5 25 32 30 34 27 19 CCASCN2 replicate 0.00001 090894C "54,315" "±2,211" 4.1% "51,032" 106.43 Replicate of different thin section. Essentially no unmyelinated fibers. Either invisble or not present. May-7-95 8 "Box 33, Slot Q-2" "13,075 x" 193 x RCS RW and RW "1,906 mm^2" "5,270 mm^2" 30.8 µm^2 1.27% 1655 21 689 32.8 ±5.97 ±1.34 42 36 32 39 30 36 30 38 32 29 21 35 40 30 33 43 33 22 34 30 24 CCASCN2 M 1 8 090894D "62,927" "±6,506" "62,927" "±6,506" 10.3% "44,060" 142.82 0.584 111 ? 940804 940908 35 19.5 500 0 1 "Backcross progeny (BALB/c x CAST/Ei) x BALB/c. Body weight 19.5 gm, not 9.5 gm. Albino" "Cage 4, case 111" "Tissue, not extracted" Dec-7-94 10 "Box 33, Slot R-1" 3 "13,037 x" 246 x RCS RCS and RW "2,674 mm^2" "5,418 mm^2" 31.9 µm^2 1.23% 1382 17 774 45.5 ±18.83 ±4.71 1.5797E-03 2.5103E-08 74459 52672 70.7% " 21,787 " 42 41 51 105 45 57 42 51 38 49 51 31 21 45 16 52 37 CCASCN2 F 1 8 062194K "63,409" "±5,056" "63,409" "±5,056" 8.0% "51,194" 123.86 0.710 ? 940517 940621 35 18.0 446 1 5 Agouti N2 ON29 1 necrotic on 0010 "Tissue, not extracted" Dec-23-94 1 "Box 35, Slot T" 4 "16,122 x" 193.17 x THK RW and RW "1,910 mm^2" "5,418 mm^2" 20.8 µm^2 0.90% 2456 22 568 25.8 ±9.43 ±2.06 2.5982E-03 4.0975E-08 64301 62514 97.2% " 1,786 " 28 24 29 29 28 27 25 46 33 13 35 31 30 31 24 29 26 30 8 26 14 2 CCASCN2 F 1 8 062194L "63,252" "±3,161" "63,252" "±3,161" 5.0% "44,194" 143.12 0.669 ? 940516 940621 36 15.0 467 1 5 Albino N2 ON30 none "Tissue, not extracted" Dec-23-94 2 "Box 35, Slot A-1" 4 "16,122 x" 193.17 x THK RW and RW "1,649 mm^2" "5,418 mm^2" 20.8 µm^2 0.85% 2120 18 537 29.8 ±6.15 ±1.49 6.7016E-03 1.0595E-07 61719 64781 105.0% " 3,062 " 35 23 25 30 35 27 38 31 29 34 34 39 37 32 26 20 21 21 CCASCN2 F 1 8 062194N "62,052" "±5,646" "62,052" "±5,646" 9.1% "66,520" 93.28 0.357 ? 940516 940621 36 15.0 448 1 5 Albino N2 ON32 none "Tissue, not extracted" Dec-23-94 4 "Box 35, Slot B-4" 4 "16,122 x" 193.17 x THK RW and RW "2,482 mm^2" "5,418 mm^2" 20.8 µm^2 0.56% 3191 18 350 19.4 ±7.29 ±1.77 2.0614E-03 3.3221E-08 67369 56732 84.2% " 10,637 " 18 13 13 12 13 20 16 18 20 29 8 13 23 26 23 35 31 19 CCASCN2 F 042694F 940331 940426 26 14.0 476 2 First N2 from Cage 2 (p265) "Tissue, not extracted" CCASCN2 M 052094E 5 940420 940520 30 15.8 479 3 Albino N2. Raised by foster mother. "From Cage 2, case 5" "Tissue, not extracted" CCASCN2 F 052094F 6 940420 940520 30 15.0 473 3 Albino N2. Raised by foster mother. "From Cage 2, case 6" "Tissue, not extracted" #NUM! CCASCN2 M 052094G 7 940420 940520 30 17.5 503 3 Albino N2. Raised by foster mother. "From Cage 2, case 7" "Tissue, not extracted" #NUM! CCASCN2 F 052094H 11 940420 940520 30 13.0 454 3 Agouti N2. Raised by foster mother. "From Cage 2, case 11" "Tissue, not extracted" #NUM! CCASCN2 M 052094I 9 940420 940520 30 16.8 475 3 Agouti N2. Raised by foster mother. "From Cage 2, case 9" "Tissue, not extracted" #NUM! CCASCN2 M 052094J 10 940420 940520 30 15.3 452 3 Agouti N2. Raised by foster mother. "From Cage 2, case 10" "Tissue, not extracted" #NUM! CCASCN2 M 052094K 940420 940520 30 15.5 459 4 Agouti N2 "Cage 2, case 8" "Tissue, not extracted" #NUM! CCASCN2 M 052094L 940420 940520 30 17.3 493 4 Agouti N2 "Cage 2, case 12" "Tissue, not extracted" #NUM! CCASCN2 M 052094M 940420 940520 30 11.5 434 4 Agouti N2 "Cage 2, case 13" "Tissue, not extracted" #NUM! CCASCN2 M 052094N 940420 940520 30 11.0 423 4 Agouti N2 "Cage 2, case 14" "Tissue, not extracted" #NUM! CCASCN2 M 052094O 940420 940520 30 14.0 441 "Tissue, not extracted" CCASCN2 M 052094P 940420 940520 30 15.8 439 "Tissue, not extracted" CCASCN2 F 052094Q 940420 940520 30 12.0 457 "Tissue, not extracted" CCASCN2 F 052094R 940420 940520 30 14.0 440 "Tissue, not extracted" CCASCASN2 M 1 8 021795N "56,673" "±2,970" "±6,642" 6 "60,018" "±2,746" "60,018" "±2,746" 4.6% "64,801" 92.62 0.504 417 950111 950217 37 20.0 475 0 1 Case 138. CCASF1 females #35 and 36 were mated to CAST/Ei male #3 to produce these four progeny. Agouti color Jun-16-95 10 "Box 46, Slot O-2" 3 "12,846 x" 247 x RCS RW and RW "3,946 mm^2" "5,380 mm^2" 32.6 µm^2 1.26% 1987 25 755 30.2 ±6.77 ±1.38 8.2888E-03 1.3810E-07 32 33 24 28 28 32 24 24 25 33 28 24 26 25 37 35 40 30 36 18 28 51 28 29 37 CCASCASN2 F 1 8 021795O "51,462" "±2,564" "51,462" "±2,564" 5.0% "61,622" 83.51 -0.785 950111 950217 37 14.0 415 1 1 Case 139. Agouti color Jun-16-95 13 "Box 46, Slot P-1" 3 "12,951 x" 246 x RCS RW and RW "3,736 mm^2" "5,380 mm^2" 32.1 µm^2 0.99% 1921 19 509 26.8 ±5.66 ±1.33 8.2637E-03 1.6058E-07 31 30 34 30 26 24 28 32 25 20 22 26 25 23 40 24 32 21 16 CCASCASN2 M 1 8 021795P "64,895" "±3,339" "64,895" "±3,339" 5.1% "69,499" 93.37 1.238 950111 950217 37 20.0 456 0 1 Case 140. Agouti color Jun-19-95 15 "Box 46, Slot Q-2" "12,992 x" 191 x RCS RCS and RCS "2,543 mm^2" "5,380 mm^2" 31.9 µm^2 0.96% 2180 21 625 29.8 ±6.85 ±1.53 6.1129E-03 9.4197E-08 49 32 26 32 22 31 30 24 21 23 20 20 31 30 29 35 32 33 37 35 33 CCASCASN2 F 1 8 021795Q "50,583" "±2,598" "50,583" "±2,598" 5.1% "43,110" 117.33 -0.917 950111 950217 37 13.5 385 1 1 Case 141. CCASF1 females #35 and 36 were mated to CAST/Ei male #3 to produce these four progeny. Agouti color none Jun-19-95 17 "Box 46, Slot R-1" 3 "12,941 x" 248 x RCS RCS and RCS "2,645 mm^2" "5,380 mm^2" 32.1 µm^2 1.71% 1342 23 867 37.7 ±9.08 ±1.94 7.8322E-03 1.5484E-07 51 41 51 35 30 25 44 50 31 42 44 28 33 35 23 39 41 43 52 33 24 28 44 CCASCASN2 F 1 8 022795A "50,317" "±2,738" "50,317" "±2,738" 5.4% "49,125" 102.43 -0.957 950118 950227 40 12.5 406 1 2 Case 143. CCASF1 females #35 and 36 were mated to CAST/Ei male #3 to produce these four progeny. Agouti color Jun-19-95 "12,855 x" 247 x RCS RCS and RCS "3,008 mm^2" "5,380 mm^2" 32.6 µm^2 1.33% 1509 20 667 33.4 ±7.91 ±1.81 7.0671E-03 1.4045E-07 50 45 38 42 34 30 40 43 23 30 31 32 23 27 33 32 21 37 24 32 CCASCASN2 M 1 8 022795B "62,766" "±3,705" "62,766" "±3,705" 5.9% "79,592" 78.86 0.917 950118 950227 40 17.5 445 0 2 Case 144. CCASF1 females #35 and 36 were mated to CAST/Ei male #3 to produce these progeny. Agouti color Jun-19-95 "12,984 x" 246 x RCS RCS and RCS "4,820 mm^2" "5,380 mm^2" 31.9 µm^2 0.96% 2494 24 604 25.2 ±7.12 ±1.49 4.7717E-03 7.6024E-08 25 24 18 20 12 38 37 37 22 34 25 23 34 21 20 16 27 34 20 20 20 24 28 25 CCASCASN2 M 022795C 950118 950227 40 17.5 443 2 Case 145. CCASF1 females #35 and 36 were mated to CAST/Ei male #3 to produce these four progeny. Agouti color CCASCASN2 M 022795D 950118 950227 40 15.5 415 2 Case 146. CCASF1 females #35 and 36 were mated to CAST/Ei male #3 to produce these four progeny. Agouti color CCASCASN2 M 022795E 950118 950227 40 18.3 412 2 Case 147. CCASF1 females #35 and 36 were mated to CAST/Ei male #3 to produce these four progeny. Agouti color CCASCASN2 M 022795F 950118 950227 40 14.0 385 2 Case 148. CCASF1 females #35 and 36 were mated to CAST/Ei male #3 to produce these four progeny. Agouti color CCASCASN2 M 022795G 950118 950227 40 14.5 390 2 Case 149. CCASF1 females #35 and 36 were mated to CAST/Ei male #3 to produce these four progeny. Agouti color CCASCASN2 M 022795H 950118 950227 40 15.5 421 2 Case 150. CCASF1 females #35 and 36 were mated to CAST/Ei male #3 to produce these four progeny. Agouti color CCASCASN2 F 022795I 950118 950227 40 11.8 387 2 Case 151. CCASF1 females #35 and 36 were mated to CAST/Ei male #3 to produce these four progeny. Agouti color CCASCASN2 F 022795J 950118 950227 40 12.5 392 2 Case 152. CCASF1 females #35 and 36 were mated to CAST/Ei male #3 to produce these four progeny. Agouti color CCASCASN2 M 022795K 950118 950227 40 12.3 368 2 Case 153. CCASF1 females #35 and 36 were mated to CAST/Ei male #3 to produce these four progeny. Agouti color CCASCASN2 M 022795L 950118 950227 40 14.1 402 2 Case 154. CCASF1 females #35 and 36 were mated to CAST/Ei male #3 to produce these four progeny. Agouti color CCASCASN2 M 022795M 950111 950227 47 21.5 484 3 Case 135 Agouti color CCASCASN2 M 022795N 950111 950227 47 22.5 440 4 Case 136 Agouti color CCASCASN2 M 022795O 950111 950227 47 16.5 427 4 Case 137 Agouti color CCASCASN2 M 022795P 950122 950227 36 16.1 440 4 Case 164 Agouti color CCASCASN2 M 022795Q 950122 950227 36 16.5 433 4 Case 165 Agouti color CCASCASN2 F 022795R 950122 950227 36 15.3 436 4 Case 166 Agouti color CCASCASN2 M 022795S 950122 950227 36 16.5 419 4 Case 167 Agouti color CCASCASN2 F 022795T 950122 950227 36 11.5 372 4 Case 168 Agouti color CCASCASN2 F 022795U 950122 950227 36 14.0 422 4 Case 169 Agouti color CCASCASN2 F 022795V 950122 950227 36 13.0 383 4 Case 170 Agouti color CD1 M 1 9 910311A "68,338" "±2,163" "±7,720" 14 "65,877" "±3,069" "65,877" "±3,069" 4.7% "68,014" 96.86 -0.319 Dan Goldowitz colony L 910311 100 NA 0 Reshot by Denny at higher magnification. Age only approximate. Adult from Dan's colony. none Dec-29-94 A4 "Box 11, Slot I-1" 3 "13,236 x" 192 x DR RW and RW "2,517 mm^2" "5,418 mm^2" 30.9 µm^2 1.00% 2199 22 659 30.0 ±6.40 ±1.40 7.3259E-03 1.1121E-07 65375 66375 101.5% " 1,000 " 34 34 36 25 34 29 31 28 33 26 20 41 36 24 24 32 28 28 15 26 41 34 CD1 M 1 9 910311B "69,171" "±2,430" "69,171" "±2,430" 3.5% "77,461" 89.30 0.108 Dan Goldowitz colony R 910311 100 NA 0 Age only approximate. Adult from Dan's colony. May-2-91 A6 "Box 12, Slot H-8" 4 "11,117 x" 387 x DT DT and DT and RW "11,601 mm^2" "5,366 mm^2" 43.4 µm^2 1.96% 1784 35 1357 38.8 ±7.94 ±1.36 1.2061E-02 1.7437E-07 70626 67323 95.3% " 3,303 " 33 44 39 47 37 44 47 42 38 55 48 36 24 35 44 32 33 38 34 53 28 31 30 42 23 37 50 35 26 36 42 47 45 45 37 CD1 M 1 9 910311C "65,408" "±2,831" "65,408" "±2,831" 4.3% "62,727" 104.27 -0.380 Dan Goldowitz colony R 910311 100 NA 0 Age only approximate. Adult from Dan's colony. May-14-91 B4 "Box 13, Slot A-1" 4 "11,237 x" 372 x DT DT and DT and RW "8,679 mm^2" "5,324 mm^2" 42.2 µm^2 1.81% 1488 27 1187 44.0 ±9.70 ±1.90 8.4772E-03 1.2960E-07 64717 66099 102.1% " 1,381 " 44 44 38 54 47 50 51 41 44 52 54 27 35 38 54 53 51 43 43 36 29 41 55 55 49 44 15 CD1 F 1 9 910507A "77,735" "±2,159" "77,735" "±2,159" 2.8% "99,483" 78.14 1.217 Dan Goldowitz colony R 910507 100 NA 1 Age only approximate. Adult from Dan's colony. May-22-91 A2 "Box 13, Slot P-2" 4 "11,231 x" 378 x DT JO and JO and RW "14,215 mm^2" "5,324 mm^2" 42.2 µm^2 1.95% 2357 46 1517 33.0 ±6.14 ±0.92 1.7046E-02 2.1928E-07 76762 78709 102.5% " 1,947 " 38 37 33 21 44 33 37 30 30 30 32 38 21 35 44 40 36 30 34 42 43 40 34 36 27 30 24 35 28 37 31 30 34 48 25 23 29 30 29 31 36 26 25 32 35 34 CD1 F 1 9 910507C "69,075" "±2,360" "69,075" "±2,360" 3.4% "95,772" 72.12 0.095 Dan Goldowitz colony ? 910507 100 NA 1 Age only approximate. Adult from Dan's colony. May-17-91 B4 "Box 13, Slot G-1" 4 "10,950 x" 380 x DT JO and JO and RW "13,859 mm^2" "5,324 mm^2" 44.4 µm^2 2.09% 2157 45 1441 32.0 ±7.26 ±1.09 70901 67162 94.7% " 3,739 " 33 40 28 37 15 32 49 40 23 33 40 35 37 32 28 43 36 29 32 41 32 34 37 31 33 34 24 21 12 38 34 30 28 34 29 29 37 26 20 40 37 38 31 25 24 CD1 original F 1 9 910507B "87,242" "±2,510" "88,794" "±3,341" 3.8% "82,616" 107.48 2.449 Dan Goldowitz colony #150 R 910507 100 NA 1 Age only approximate. Adult from Dan's colony. May-17-91 A6 "Box 13, Slot K-2" 4 "11,178 x" 378 x DT JO and JO and RW "11,805 mm^2" "5,324 mm^2" 42.6 µm^2 1.24% 1939 24 1099 45.8 ±8.26 ±1.72 1.4454E-02 1.6568E-07 88751 88846 100.1% 95 53 35 39 55 52 40 37 48 38 50 53 57 54 45 63 48 44 30 49 37 47 41 49 35 CD1 replicate 0.00001 910507B "85,230" "±3,802" 4.5% "90,234" 94.45 Dan Goldowitz colony #150 R 910507 NA dup Redone Oct 1994 to confirm outlier status. Different thin section examined. Low mag calibration is ok. none Oct-24-94 A6 "Box 13, Slot K-2" "13,347 x" 173 x RW RW and RW "2,686 mm^2" "5,418 mm^2" 30.4 µm^2 0.74% 2967 22 632 28.7 ±5.87 ±1.28 84958 85497 100.6% 539 30 31 30 20 20 18 30 28 21 37 37 34 32 28 23 21 28 37 30 33 34 30 CD1 original F 1 9 031493A "61,754" "±2,583" "58,631" "±3,532" 6.0% "78,786" 74.42 -0.853 "74,500 " Dan Goldowitz colony L 930315 100 481 15.4 1 Age only approximate. Adult from Dan's colony. none Oct-1-93 B6 "Box 21.5, Slot M-4" 4 "13,109 x" 192 x DR DR and RW "2,905 mm^2" "5,383 mm^2" 31.3 µm^2 1.27% 2515 32 746 23.3 ±7.82 ±1.40 9.5561E-03 1.5475E-07 56900 60358 106.1% " 3,458 " 24 22 22 26 20 24 9 33 30 39 36 26 31 10 24 32 24 7 24 17 28 8 33 20 18 25 23 27 18 28 20 18 CD1 replicate 0.00001 031493A "65,345" "±3,787" 5.8% "81,109" 80.56 Duplicate analysis of a different thin section none May-15-95 B6 "Box 21.5, Slot M-6" "13,032 x" 248 x RW RW and RW "4,993 mm^2" "5,270 mm^2" 31.0 µm^2 0.88% 2614 23 575 25.0 ±6.80 ±1.45 22 31 19 23 21 31 21 35 32 10 27 14 23 17 27 36 36 27 22 29 24 27 21 CD1 F 1 9 031493C "65,662" "±3,248" "65,662" "±3,248" 4.9% "91,332" 71.89 -0.347 "65,675 " Dan Goldowitz colony L 930315 100 NA 15.0 1 Age only approximate. Adult from Dan's colony. none May-13-93 C4 "Box 21.5, Slot N-1" 4 "13,181 x" 191 x DR DR and RW "3,337 mm^2" "5,332 mm^2" 30.7 µm^2 1.04% 2976 31 684 22.1 ±5.98 ±1.09 6.4330E-03 9.7971E-08 68072 63088 92.7% " 4,985 " 17 22 24 25 22 30 19 25 27 19 24 27 17 25 31 31 15 23 20 24 24 18 32 8 17 19 10 21 16 32 20 CD1 F 1 9 030992D "62,580" "±4,458" "62,580" "±4,458" 7.1% "82,153" 76.18 -0.746 Dan Goldowitz colony ? 920309 100 NA 1 A case cut by Lan on both glass and diamond knifes. Age only approximate. Adult from Dan's colony. Low mag cal is 598.932 lines per mm (.8835 mu per line) "All calibrations by Rob. Used grating not grid, for calibration." none Sep-2-93 B5 "Box 15, Slot K thru R" 2 "10,955 x" 473 x LN? JO and RW "18,370 mm^2" "5,105 mm^2" 42.5 µm^2 1.55% 1931 30 972 32.4 ±12.43 ±2.31 3.2581E-03 5.2063E-08 60263 64898 107.7% " 4,636 " 40 41 34 34 44 38 blank 52 40 46 47 42 14 15 11 30 16 32 36 39 53 37 42 44 31 15 24 blank 21 20 15 edge edge 19 CD1 M 1 9 013092B "62,449" "±2,548" "62,449" "±2,548" 4.1% "68,945" 90.58 -0.763 Dan Goldowitz colony ? 920130 26 24.3 NA 0 "Young male used to show Denny HRP injection protocol. Marginal tissue, but count is probably pretty good (if anything a bit low)." Sample area is 28.4 square micrometers. Direct determination. Hard to count unmyelinated fibers. none Sep-21-93 A2 "Box 20, Slot T-1" 2 "13,596 x" 197 x DR DR and RW "2,677 mm^2" "5,324 mm^2" 28.4 µm^2 1.19% 2428 29 746 25.7 ±5.55 ±1.05 9.9610E-03 1.5951E-07 56643 68666 121.2% " 12,022 " 28 29 32 33 17 30 25 23 21 31 20 25 27 28 10 24 25 30 33 34 17 28 19 23 23 31 26 27 27 CD1 F 1 9 900308.#10 "73,600" "±3,698" "73,600" "±3,698" 5.0% "84,605" 86.99 0.682 Dan Goldowitz colony 10 L 900308 100 1 Also known as ICR#10. Age only approximate. Adult from Dan's colony. Redone by Rob Sept 94. Count is fine. "Low contrast negatives, but shot at higher magnification. " Sep-19-94 B5 "Box 4, Slot A" 3 "16,160 x" 246 x RW RW and RW "5,118 mm^2" "5,418 mm^2" 20.7 µm^2 0.51% 4078 21 379 18.0 ±4.06 ±0.91 5.6504E-03 7.6773E-08 67694 78964 116.6% " 11,270 " 24 27 18 15 20 14 18 20 20 9 22 17 20 16 19 15 20 20 13 13 19 CD1 F 1 9 901018C "64,917" "±2,965" "64,917" "±2,965" 4.6% "73,565" 88.24 -0.443 Dan Goldowitz colony R 901018 100 1 Adult female Very low contrast micrographs photographed by Denny Dec-29-94 "Box 9, Slot I-3" 2 "16,156 x" 192 x DR RW and RW "2,722 mm^2" "5,418 mm^2" 20.8 µm^2 0.62% 3544 22 403 18.3 ±3.83 ±0.84 65076 64754 99.5% 322 23 21 24 18 26 19 10 18 13 21 20 20 18 21 13 14 16 17 19 15 20 17 CD1 original F 1 9 900208.1 "57,243" "±2,731" "59,878" "±3,578" 6.0% "54,463" 109.94 -1.437 Dan Goldowitz colony 1 L 900208 100 1 "First analysis by Daphne. Box 10 slot Q2. Possibly a slight undercount due to low power of sample. All calibrations modernized by Rob, Jan 1995." 1 necrotic on 0007 Dec-13-91 C5 "Box 10, Slot Q-2" 3 "10,986 x" 385 x DT 2-13-91 "DT, DT and RW" "8,078 mm^2" "5,324 mm^2" 41.7 µm^2 1.99% 1306 26 1192 45.8 ±13.70 ±2.74 7.9829E-03 1.3946E-07 59575 62782 105.4% " 3,208 " 74 57 39 43 57 53 55 56 41 47 47 46 78 34 39 42 33 56 40 53 40 28 13 52 37 32 CD1 replicate 0.00001 900208.1 "53,564" "±4,227" 7.9% "54,874" 97.61 Replicate of a different thin section. none May-5-95 C5 "Box 10, Slot Q-6" 2 "13,143 x" 206 x RW RW and RW "2,329 mm^2" "5,270 mm^2" 30.5 µm^2 1.28% 1799 23 685 29.8 ±11.02 ±2.35 9 17 29 29 25 26 41 35 34 33 26 25 30 35 29 55 15 38 38 9 48 34 25 CD1 original F 1 9 900208.2 "74,025" "±2,703" "74,517" "±3,065" 4.1% "63,743" 116.90 0.737 Dan Goldowitz colony 3 R mid orbital 900208 100 1 Feb-12-91 A5 "Box 10, Slot M-2" 3 "10,967 x" 385 x DT DT and DT "9,468 mm^2" "5,033 mm^2" 41.8 µm^2 1.58% 1523 24 1174 48.9 ±9.65 ±2.01 1.0574E-02 1.4285E-07 76546 72484 94.7% " 4,062 " 50 50 59 60 61 26 46 28 45 59 55 49 52 48 33 40 49 48 43 48 58 54 52 61 CD1 replicate 0.00001 9 900208.2 "74,833" "±5,730" 7.7% "67,594" 110.71 Dan Goldowitz colony 3 R mid orbital 900208 dup Redone by Toya at standard magnificiations none Mar-6-95 A5 "Box 10, Slot M-4" "12,999 x" 193 x TK RW and RW "2,515 mm^2" "5,418 mm^2" 32.1 µm^2 0.95% 2108 20 710 35.5 ±11.85 ±2.72 85863 62105 72.3% " 23,758 " 28 29 35 40 27 32 37 58 30 23 29 38 37 32 41 50 18 52 16 58 CD1 replicate 2 0.00001 9 900208.2 "73,592" "±2,371" 3.2% "59,821" 123.02 Dan Goldowitz colony 3 R mid orbital 900208 dup Mar-7-91 A5 "Box 10, Slot M-6" "11,571 x" 391 x DT DT and DT and RW "9,145 mm^2" "5,033 mm^2" 37.6 µm^2 1.57% 1592 25 1156 46.2 ±7.30 ±1.49 74309 72810 98.0% " 1,500 " edge 56 46 40 48 38 39 46 46 43 46 54 42 44 66 40 45 41 39 edge 33 44 57 50 54 53 46 CE/J original M 1 1 041493E "63,593" "±2,384" "±4,767" 5 "55,233" "±2,388" "59,156" "±4,407" 7.4% "66,124" "15,227" 89.46 -1.753 JR000657 ? 464 930223 930414 50 18.8 see notes 0 1 "Careful with ID number. A black-eyed, greyish white inbred strain derived from wild mice trapped by J.E. Knight in 1920. Probably crossed to lab mice at some point. Brain weight recorded as 0.261 gm." This individual had a kinked tail. none Oct-1-93 D6 "Box 22, Slot A-1" 4 "13,307 x" 192 x DR and THK RW and RW "2,432 mm^2" "5,352 mm^2" 30.2 µm^2 1.14% 2188 25 676 27.0 ±9.87 ±2.01 1.0090E-02 1.8267E-07 68700 55243 80.4% " 13,457 " 9 34 34 22 14 40 43 25 26 27 34 29 39 22 47 30 31 32 28 15 21 21 23 8 22 CE/J replicate 0.00001 041493E "57,469" "±2,823" 4.9% "66,400" 86.55 Replicate count of a different thin section 2 necrotic fibers May-3-95 D6 "Box 22, Slot A-1" "12,873 x" 247 x RW RW and RW "4,065 mm^2" "5,270 mm^2" 31.8 µm^2 1.01% 2088 21 578 27.5 ±6.05 ±1.35 19 36 27 37 17 28 38 24 22 37 28 24 30 26 23 32 20 25 30 25 30 CE/J other side 0.00001 041493E "52,506" "±4,892" 9.3% "72,773" 72.15 D5 "Box 49, Slot N-1" "12,395 x" 193 x SS SS and RW "2,700 mm^2" "5,270 mm^2" 34.3 µm^2 0.94% 2121 20 495 24.8 ±10.05 ±2.31 29 19 24 37 41 19 45 15 30 14 13 36 25 28 22 16 25 6 31 20 CE/J M 1 1 041493F "66,888" "±3,394" "63,040" "±4,559" 7.2% "75,647" "12,562" 83.33 0.691 JR000657 ? 930223 930414 50 20.3 see notes 0 1 "A black-eyed, greyish white inbred strain derived from wild mice trapped by J.E. Knight in 1920. Probably crossed to lab mice at some point. Brain weight recorded as 0.265 gm." "cauliflower right ear. Montage from Grid B3, sample photos from Grid B2. Checked area of sections and they are the same. Good data" none Oct-1-93 A3 "Box 22, Slots B-2 and B-3" 4 "12,819 x" 249 x TK TK and RW "4,673 mm^2" "5,393 mm^2" 32.8 µm^2 0.87% 2305 20 547 27.4 ±8.62 ±1.98 6.1132E-03 9.1396E-08 52255 76564 146.5% " 24,310 " 25 24 33 7 41 28 22 not available 34 19 33 22 26 not in nerve 30 25 49 26 32 24 26 21 CE/J other side 041493F "71,669" "±5,082" 7.1% "74,115" "18,779" 96.70 "16,039 x" 191 x SS SS and RW "2,693 mm^2" "5,270 mm^2" 20.5 µm^2 0.58% 3618 21 416 19.8 ±6.28 ±1.40 25 16 11 26 22 30 16 18 14 13 25 18 24 17 28 33 20 12 18 12 18 CE/J F 1 1 041393E "64,678" "±4,016" "64,678" "±4,016" 6.2% "79,893" 80.96 0.228 JR000657 ? 930223 930414 50 19.5 461 1 1 "Careful with ID number. A black-eyed, greyish white inbred strain derived from wild mice trapped by J.E. Knight in 1920. Probably crossed to lab mice at some point." A second set of sample photos taken (4 to 11) to finish covering the nerve. Low contrast negatives. none Oct-13-92 C3 "Box 22, Slot I-1" 4 "12,919 x" 249 x TK TK and RW "4,972 mm^2" "5,409 mm^2" 32.4 µm^2 1.38% 2465 34 892 26.2 ±9.36 ±1.63 4.1313E-03 6.3875E-08 64913 63822 98.3% " 1,091 " 41 22 27 39 22 not in nerve 9 23 12 29 38 31 26 34 22 10 31 8 22 14 30 34 36 30 17 29 15 28 34 31 16 25 39 29 39 CE/J original M 1 1 082094A "66,316" "±2,202" "68,940" "±3,885" 5.6% "91,842" 75.06 0.571 JR000657 R or L 940620 940820 61 29.0 470 0 2 Dark eyes and chinchilla coat color. Negatives used on 1994 Neuroscience poster. Cannot find now. A few necrotic Oct-30-94 1 "Box 20, Slot B-2" 4 "12,979 x" 193 x DR RW and RW "3,408 mm^2" "5,418 mm^2" 32.2 µm^2 0.74% 2855 21 507 24.1 ±6.09 ±1.36 1.4363E-02 2.1659E-07 69196 71741 103.7% " 2,545 " 21 14 25 20 26 20 18 23 14 28 32 21 34 30 27 27 21 36 17 27 26 CE/J replicate 0.00001 082094A "68,875" "±4,109" 6.0% "87,386" 78.82 R or L Replicate of a different section 1 necrotic May-5-95 1 "Box 20, Slot B-2" "10,792 x" 206 x RW RW and RW "3,724 mm^2" "5,270 mm^2" 45.3 µm^2 1.09% 1931 21 749 35.7 ±9.51 ±2.13 54 33 32 42 29 30 62 39 40 24 27 22 35 34 30 35 36 33 27 41 44 CE/J other side 082094A "63,723" "±3,414" 5.4% "86,259" 73.87 L or R Other side by Stacie none Jul-19-95 2 "Box 50, Slot K-2" "12,906 x" 193 x SS SS and RW "3,210 mm^2" "5,270 mm^2" 31.6 µm^2 0.88% 2726 24 561 23.4 ±6.01 ±1.25 23 12 23 28 23 19 29 17 24 33 21 19 17 23 26 18 22 28 29 34 21 12 31 29 CE/J M 121995N 951219 200 25.0 439 CE/J F 121994O 951219 200 28.5 457 CE/J M 1 1 082094B "64,848" "±3,745" "64,848" "±3,745" 5.8% "83,106" 78.03 0.263 JR000657 940620 940820 61 26.0 485 0 2 "Poor fixation according to perfusion notes, but curiously the tissue is great." 1 necrotic on 0007 Oct-30-94 3 "Box 20, Slot C-1" 4 "13,143 x" 192 x DR RW and RW "3,066 mm^2" "5,418 mm^2" 31.4 µm^2 0.79% 2649 21 514 24.5 ±6.32 ±1.41 4.8547E-03 7.4863E-08 62076 66999 107.9% " 4,923 " 32 11 22 18 17 26 17 32 26 27 29 33 25 26 34 23 29 23 19 16 29 CE/J poor fixation M 082094C 940620 940820 61 26.0 474 2 Listed as OK fixation. Not good enough to count. CZECHII/Ei M 1 4 052793C "59,207" "±3,341" "±8,183" 7 "49,312" "±3,556" "49,312" "±3,556" 7.2% "79,344" 62.15 -1.209 JR1144 ? 350 NA 930527 200 19.0 366 0 1 Mus musculus musculus. Retired breeder Original calibration is somewhat out of focus. So is the montage. No compenstation is appropriate. none noted Aug-31-93 B6 "Box 25.5, Slot M-1" 2 "12,935 x" 199 x RW RW and RW "3,129 mm^2" "5,410 mm^2" 32.3 µm^2 0.86% 2454 21 422 20.1 ±6.48 ±1.45 4.0955E-03 8.3053E-08 53428 54949 102.8% " 1,521 " 25 12 22 21 10 13 20 15 33 27 17 29 20 21 11 17 18 20 16 23 32 CZECHII/Ei please reshoot 1E-08 052793C CZECHII/Ei F 1 4 052793D "60,961" "±3,389" "60,961" "±3,389" 5.6% "127,932" 47.65 0.214 JR1144 ? NA 930527 200 18.0 372 1 1 Mus musculus musculus. Retired breeder 1 necrotic Aug-31-93 C4 "Box 25.5, Slot N-1" 3 "12,943 x" 195 x RW RW and RW "4,841 mm^2" "5,410 mm^2" 32.3 µm^2 0.91% 3961 36 554 15.4 ±5.06 ±0.86 5.4596E-03 8.9559E-08 61189 62079 101.5% 890 19 18 22 21 16 20 18 18 17 16 16 27 14 22 8 4 23 12 18 13 6 11 8 15 12 22 18 17 8 14 14 15 13 15 13 11 CZECHII/Ei F 1 4 051394K "54,036" "±4,047" "54,036" "±4,047" 7.5% "77,210" 69.99 -0.632 JR1144 ? 940309 940513 65 8.5 353 1 2 Mus musculus musculus. none Aug-11-94 13 "Box 27, Slot S-2" 3 "13,074 x" 250 x RW RW and RW "4,807 mm^2" "5,443 mm^2" 31.8 µm^2 0.87% 2425 21 468 22.3 ±7.46 ±1.67 3.4643E-03 6.4111E-08 54870 60234 109.8% " 5,365 " 16 29 31 19 29 18 8 16 33 31 24 12 19 20 24 25 29 11 16 31 27 CZECHII/Ei F 1 4 051394L "56,846" "±5,555" "56,846" "±5,555" 9.8% "68,514" 82.97 -0.288 JR1144 ? 940309 940513 65 8.5 328 1 2 Mus musculus musculus. none Aug-11-94 15 "Box 27, Slot T-1" 3 "13,055 x" 248 x RW RW and RW "4,218 mm^2" "5,443 mm^2" 31.9 µm^2 0.93% 2145 20 530 26.5 ±11.29 ±2.59 1.9390E-03 3.4110E-08 61089 62018 101.5% 929 13 14 10 13 34 24 30 50 43 30 32 15 10 31 25 28 34 36 36 22 CZECHII/Ei F 1 4 020795A "54,381" "±3,778" "54,381" "±3,778" 6.9% "64,752" 83.98 -0.590 JR1144 ? 941221 950207 48 9.0 340 1 3 This case was labeled correctly. 1 necrotic May-16-95 1 "Box 44, Slot F-2" 3 "12,929 x" 247 x RW RW and RW "3,951 mm^2" "5,270 mm^2" 31.5 µm^2 1.02% 2054 21 556 26.5 ±8.23 ±1.84 4.0007E-03 7.3569E-08 17 37 20 5 24 29 19 30 20 32 16 28 22 36 29 33 30 34 33 37 25 CZECHII/Ei M 1 4 020795H "73,647" "±3,680" "73,647" "±3,680" 5.0% "79,893" 92.18 1.765 JR1144 ? 941221 950207 48 12.6 351 0 3 Once listed at PANCEVO/Ei but actually CZECHII none May-16-95 16 "Box 44, Slot M-2" 3 "12,943 x" 193 x RW RW and RW "2,990 mm^2" "5,270 mm^2" 31.5 µm^2 0.87% 2540 22 638 29.0 ±6.64 ±1.45 5.6974E-03 7.7361E-08 31 30 33 26 31 21 20 23 39 28 10 27 34 29 33 24 29 33 40 30 33 34 CZECHII/Ei please reshoot 1E-08 020795H CZECHII/Ei M 1 4 020795I "65,265" "±3,553" "65,265" "±3,553" 5.4% "79,376" 82.22 0.740 JR1144 ? 941221 950207 48 11.3 338 0 3 Once listed at PANCEVO/Ei but actually CZECHII May-17-95 18 "Box 44, Slot N-1" 4 "13,004 x" 194 x RW RW and RW "2,995 mm^2" "5,270 mm^2" 31.2 µm^2 0.94% 2547 24 615 25.6 ±6.69 ±1.40 5.3940E-03 8.2647E-08 11 29 17 15 26 21 19 24 28 26 21 33 34 30 26 31 13 29 33 32 28 28 27 34 DBA/2J M 1 1 061193A "63,351" "±1,137" "±4,100" 13 "62,006" "±4,230" "62,006" "±4,230" 6.8% "85,536" 72.49 -0.328 JR000671 ? 394 930421 930611 51 15.3 360 1 "Second count. Reshot by Denny Dec 1994 to confirm initial count of 56,373. Fine count." Fixation is not great 1 necrotic fiber Dec-13-94 A2 "Box 22, Slot Q-2" 2 "13,146 x" 196 x DR RW and RW "3,286 mm^2" "5,418 mm^2" 31.4 µm^2 0.81% 2728 22 500 22.7 ±7.11 ±1.55 3.6295E-03 5.8536E-08 64465 58925 91.4% " 5,540 " 24 29 26 35 29 17 20 29 26 22 18 0 28 17 22 16 23 28 21 28 24 18 DBA/2J M 1 1 061193B "57,883" "±4,009" "57,883" "±4,009" 6.9% "85,539" 67.67 -1.334 JR000671 ? 930421 930611 51 19.5 395 0 1 All calibrations redone by Rob March 1995. none Oct-28-93 A5 "Box 22, Slot R-1" 3 "12,966 x" 251 x TK TK and RW and RW "5,379 mm^2" "5,380 mm^2" 32.0 µm^2 0.97% 2673 26 563 21.7 ±7.50 ±1.50 3.7453E-03 6.4704E-08 55435 67476 121.7% " 12,041 " 22 30 21 8 23 35 33 22 13 25 17 21 25 28 27 21 3 22 13 21 12 31 22 21 19 28 DBA/2J F 1 1 061193C "64,731" "±3,250" "64,731" "±3,250" 5.0% "90,146" 71.81 0.337 JR000671 ? 930421 930611 51 16.3 392 1 1 All calibrations redone by Rob March 1995. none Oct-28-93 B2 "Box 22, Slot S-2" 2 "12,966 x" 251 x TK TK and RW and RW "5,669 mm^2" "5,376 mm^2" 32.0 µm^2 0.99% 2819 28 643 23.0 ±5.99 ±1.15 6.3563E-03 9.8195E-08 65831 64766 98.4% " 1,065 " see to the right 15 27 33 28 18 15 22 22 23 28 32 15 17 12 27 28 13 24 26 32 22 26 29 16 22 22 22 27 DBA/2J original F 1 1 061193D "65,496" "±3,472" "68,722" "±4,651" 6.8% "102,454" 67.08 0.523 JR000671 ? 930421 930611 51 16.8 367 1 1 All calibrations redone by Rob March 1995. none Nov-1-93 B5 "Box 22, Slot T-1" 3 "12,959 x" 248 x TK TK and RW and RW "6,305 mm^2" "5,373 mm^2" 32.0 µm^2 0.81% 3202 26 558 21.5 ±7.26 ±1.45 5.6489E-03 8.6248E-08 77801 62468 80.3% " 15,333 " 34 19 18 31 26 28 28 34 19 16 24 23 8 23 4 22 10 29 25 20 22 21 17 20 16 21 DBA/2J replicate 0.00001 061193D "61,433" "±5,220" 8.5% "103,149" 59.56 Replicate of a different thin section May-3-95 B5 "Box 22, Slot T-1" "12,837 x" 192 x RW RW and RW "3,795 mm^2" "5,270 mm^2" 32.0 µm^2 0.68% 3226 22 419 19.0 ±7.42 ±1.62 6 21 19 22 22 12 17 20 7 12 23 29 7 17 26 23 28 23 16 14 35 20 DBA/2J original F 1 1 071593A "66,919" "±3,861" "64,282" "±4,838" 7.5% "139,870" 45.96 0.870 JR000671 ? 930511 930715 65 Not perfused here 1 2 Perfused at Jackson. No brain weight data none Mar-3-94 C4 "Box 25, Slot A-1" 3 "12,945 x" 247 x TK TK and RW "8,511 mm^2" "5,449 mm^2" 32.5 µm^2 0.81% 4302 35 523 14.9 ±6.56 ±1.12 4.6201E-03 6.9040E-08 59065 74213 125.6% " 15,147 " 23 17 18 9 7 12 17 6 27 19 13 9 23 19 19 13 9 6 13 28 18 9 20 9 8 16 11 24 29 13 12 15 6 18 8 DBA/2J replicate 0.00001 071593A "71,549" "±6,410" 9.0% "131,461" 54.43 Duplicate analysis of the original thin section. none May-15-95 C4 "Box 25, Slot A-1" 3 "13,124 x" 192 x RW RW and RW "4,836 mm^2" "5,270 mm^2" 30.6 µm^2 0.54% 4297 23 383 16.7 ±7.00 ±1.49 18 23 31 22 6 4 20 14 2 18 16 13 13 17 26 12 13 19 17 13 19 27 20 DBA/2J F 1 1 071593B "58,996" "±3,709" "58,996" "±3,709" 6.3% "112,293" 52.54 -1.062 JR000671 ? 930511 930715 65 Not perfused here 1 2 Perfused at Jackson. No brain weight data All calibrations redone by Rob March 1995. none Mar-4-94 D1 "Box 25, Slot B-2" 3 "12,925 x" 248 x TK TK and RW "6,928 mm^2" "5,447 mm^2" 32.6 µm^2 0.90% 3444 31 531 17.1 ±5.90 ±1.08 4.4324E-03 7.5131E-08 62230 66231 106.4% " 4,001 " 23 9 19 19 12 18 14 13 24 14 11 18 10 23 20 8 24 20 23 20 11 19 25 2 13 16 13 21 24 26 19 DBA/2J F 1 1 071593C "65,955" "±5,827" "65,955" "±5,827" 8.8% "107,708" 61.23 0.635 JR000671 ? 930511 930715 65 Not perfused here 1 2 Perfused at Jackson. No brain weight data none Mar-4-94 D4 "Box 25, Slot C-1" 3 "12,985 x" 247 x TK TK and RW "6,546 mm^2" "5,443 mm^2" 32.3 µm^2 0.78% 3336 26 514 19.8 ±8.73 ±1.75 2.0202E-03 3.0629E-08 69729 74790 107.3% " 5,061 " 27 6 16 8 10 25 15 18 33 42 7 14 24 23 25 22 25 29 11 17 23 11 19 18 31 15 DBA/2J original F 1 1 071593D "57,625" "±2,480" "56,785" "±3,310" 5.8% "77,720" 73.06 -1.397 JR000671 ? 930511 930715 65 Not perfused here 1 2 1 necrotic Mar-7-94 D6 "Box 25, Slot D-2" 2 "12,977 x" 247 x TK TK and RW "4,759 mm^2" "5,466 mm^2" 32.5 µm^2 0.88% 2395 21 498 23.7 ±6.18 ±1.38 9.8350E-03 1.7067E-07 59392 60196 101.4% 804 32 17 30 26 31 32 19 28 20 13 16 28 24 16 29 25 29 23 18 27 15 DBA/2J replicate 0.00001 071593D "58,701" "±3,746" 6.4% "76,775" 76.46 Replicate of a different thin section none May-5-95 D6 "Box 25, Slot D-2" "12,930 x" 206 x RW RW and RW "3,249 mm^2" "5,270 mm^2" 31.5 µm^2 0.82% 2436 20 482 24.1 ±6.70 ±1.54 23 26 25 blank 31 18 35 6 20 26 21 26 20 29 20 27 27 16 36 25 25 DBA/2J M 1 1 010695F "63,026" "±3,768" "63,026" "±3,768" 6.0% "89,294" 70.58 -0.079 JR000671 ? 941128 950106 39 17.0 425 0 3 none Apr-12-95 11 "Box 42, Slot J-2" 2 "10,917 x" 193 x RCS RW and RW "3,323 mm^2" "5,270 mm^2" 44.2 µm^2 1.19% 2020 24 749 31.2 ±8.95 ±1.87 4.6311E-03 7.3479E-08 36 32 25 4 31 35 26 34 31 40 23 35 19 36 45 42 29 23 28 34 43 31 41 26 DBA/2J M 1 1 010695G "60,004" "±4,658" "60,004" "±4,658" 7.8% "70,285" 85.37 -0.816 JR000671 ? 941128 950106 39 15.0 426 0 3 none Apr-6-95 14 "Box 42, Slot D-2" 2 "16,245 x" 192 x RW RW and RW "2,579 mm^2" "5,466 mm^2" 20.7 µm^2 0.65% 3394 22 389 17.7 ±6.29 ±1.37 2.8976E-03 4.8290E-08 27 21 17 10 15 14 23 23 16 4 23 18 15 13 19 21 27 25 4 16 20 18 DBA/2J original F 1 1 010695H "61,712" "±2,209" "60,984" "±2,564" 4.2% "68,194" 89.43 -0.400 Section is torn in one corner. none Apr-12-95 16 "Box 42, Slot E-1" 2 "10,900 x" 146 x RCS RW and RW "1,445 mm^2" "5,270 mm^2" 44.4 µm^2 0.98% 1537 15 595 39.7 ±6.24 ±1.67 1.3549E-02 2.1955E-07 46 34 38 44 42 35 51 38 51 39 29 38 38 33 39 DBA/2J replicate 010695H "63,805" "±4,350" 6.8% "60,051" 106.25 JR000671 ? 941128 950106 39 14.0 401 3 Original photographs of a torn section. Block was recut and this is data from the complete section. none Apr-21-95 16 "Box 42, Slot E-1" "15,954 x" 193 x RW EGW and RW "2,244 mm^2" "5,270 mm^2" 20.7 µm^2 0.69% 2900 20 440 22.0 ±6.54 ±1.50 20 38 20 12 27 28 21 24 25 28 20 25 19 19 7 20 16 20 28 23 DBA/2J poor fixation M 013095U "78,176" JR000671 ? 941212 950130 49 17.3 370 4 Ugly tissue "ID numbers 2257, B425, order 456194" none May-24-95 10 "Box 44, Slot T-1" 1 "13,055 x" 194 x RW RW and RW "2,950 mm^2" "5,270 mm^2" 30.9 µm^2 0.87% 2528 22 480 21.8 ±6.18 ±1.35 27 25 6 24 24 22 30 23 14 6 23 22 21 26 27 25 26 21 20 21 19 28 DBA/2J M 1 1 013095V "69,429" "±4,840" "69,429" "±4,840" 7.0% "90,142" 77.02 1.483 JR000671 ? 941212 950130 49 20.5 402 0 4 "ID numbers 2257, B425, order 456194" none May-25-95 11 "Box 46, Slot A-1" 3 "12,968 x" 193 x RW RW and RW "3,354 mm^2" "5,270 mm^2" 31.3 µm^2 0.76% 2877 22 531 24.1 ±7.71 ±1.68 3.1047E-03 4.4718E-08 13 24 16 13 23 20 18 25 25 36 30 27 31 22 31 29 14 25 45 22 23 19 DBA/2J M 1 1 013095W "69,775" "±2,261" "69,775" "±2,261" 3.2% "72,952" 95.65 1.567 JR000671 ? 941212 950130 49 18.5 404 0 4 "ID numbers 2257, B425, order 456194" none May-25-95 13 "Box 46, Slot B-2" 4 "12,972 x" 248 x RW RW and RW "4,481 mm^2" "5,270 mm^2" 31.3 µm^2 0.90% 2330 21 629 30.0 ±4.34 ±0.97 1.4326E-02 2.0532E-07 27 34 33 32 27 30 27 29 30 33 30 28 37 29 40 21 35 27 25 26 29 DBA/2J-sdy/sdy M 5 062292C "78,854" "±3,296" "78,854" "±3,296" 4.2% "136,902" 57.60 JR1594 362 ? 910921 920622 275 46.0 423 17.8 0 1 Grey mouse strain. Origin from Little 1909 stock. Oldest of all inbred strains. DBA2 substain established in 1929. Has been inbred for 150 generations+ "Huge 9 month-old retired breeder mouse. Block B4=RON, Block B5=LON" Aug-12-94 B5 "Box 20A, Slot F-4" 3 "12,939 x" 176 x DR RW "4,234 mm^2" "5,387 mm^2" 32.2 µm^2 1.10% 4255 47 871 18.5 ±5.25 ±0.77 7.4160E-03 9.4047E-08 77146 80491 104.3% " 3,345 " 17 23 14 15 22 22 25 22 19 18 20 14 14 9 8 24 17 32 20 15 18 17 23 17 23 19 28 15 12 missing missing missing 21 11 12 19 18 15 13 16 21 12 21 16 30 27 14 20 24 19 DBA/2J-sdy/+ F 062292D JR1594 363 910921 920622 275 37.3 " NA, brain cut " 18.1 1 1 Marginal perfusion according to Denny's notes. Check tissue anyway. C1 "Box 20A, Slot G" DC/J-scm/scm F 121995 F 951219 300 25.5 417 1 Case 102. scrambler mutant on the DC inbred background. Agouti coat. "Date of birth may be 1/11/95, but not certain." DC/J-scm/scm F 121995G 951219 300 23.5 407 1 Case 79. scrambler mutant on the DC inbred background. Agouti coat. "Date of birth may be 1/11/95, but not certain." LP/J M 1 1 041493H "52,225" "±1,919" "±5,078" 8 "50,350" "±2,634" "50,350" "±2,634" 5.2% "73,057" 68.92 -0.369 JR000676 ? 365 930223 930414 50 20.0 see notes 0 2 Agouti with white spots. Brain weight recorded as 0.266 gm. Negatives are low contrast. Some negatives damaged a bit. Brain weight in perfusion book is incorrect. none Oct-11-93 B2 "Box 22, Slot F-1" 3 "12,598 x" 247 x TK TK and RW "4,468 mm^2" "5,398 mm^2" 34.0 µm^2 1.16% 2148 25 586 23.4 ±6.01 ±1.23 7.5591E-03 1.5013E-07 50074 46539 92.9% " 3,535 " 38 27 24 34 21 17 18 24 15 32 25 27 24 19 26 20 10 23 25 20 28 27 20 22 20 LP/J M 1 1 041493G "46,158" "±2,810" "46,158" "±2,810" 6.1% "64,682" "6,502" 71.36 -1.195 JR000676 ? 930223 930414 50 19.0 see notes 0 2 Agouti with white spots. Brain weight recorded as 0.222 gm. Agouti with white spots. Brain weight in perfusion book is incorrect. none Oct-7-93 A5 "Box 22, Slot C-3" 3 "12,936 x" 249 x TK TK and RW "4,020 mm^2" "5,372 mm^2" 32.1 µm^2 1.09% 2015 22 504 22.9 ±6.39 ±1.39 6.1237E-03 1.3267E-07 46297 53848 116.3% " 7,551 " 21 12 30 24 11 21 31 30 18 31 27 17 25 18 27 23 24 24 27 23 30 10 LP/J M 1 1 051394C "55,301" "±3,534" "55,301" "±3,534" 6.4% "63,103" 87.64 0.606 JR000676 ? 940324 940513 50 18.0 382 0 3 none Aug-19-94 18 "Box 27, Slot I-1" 4 "13,004 x" 245 x RW RW and RW "3,782 mm^2" "5,443 mm^2" 32.2 µm^2 0.97% 1960 19 536 28.2 ±7.65 ±1.80 4.6733E-03 8.4505E-08 63860 53740 84.2% " 10,121 " 22 33 25 40 26 33 28 22 18 15 26 43 36 31 33 37 25 23 20 LP/J F 1 1 051394D "51,688" "±3,699" "51,688" "±3,699" 7.2% "52,961" 97.60 -0.106 JR000676 ? 940324 940513 50 14.5 375 1 3 none Aug-19-94 19 "Box 27, Slot J-2" 4 "13,001 x" 245 x RW RW and RW "3,174 mm^2" "5,443 mm^2" 32.2 µm^2 1.28% 1645 21 660 31.4 ±10.06 ±2.25 3.9672E-03 7.6753E-08 65485 49308 75.3% " 16,178 " 41 40 33 42 24 51 25 19 31 32 24 43 36 37 27 28 6 39 20 30 32 LP/J F 1 1 091394C "50,229" "±3,557" "50,229" "±3,557" 7.1% "71,739" 70.02 -0.393 JR000676 ? 940401 940914 166 28.0 370 1 1 "Large fibers around periphery. Date perfused listed as Sept 14, 1994, but case referred to as 091394C." none Oct-13-94 8 "Box 29, Slot J-2" 3 "16,208 x" 193 x RW RW and RW "2,665 mm^2" "5,418 mm^2" 20.6 µm^2 0.72% 3478 25 361 14.4 ±5.01 ±1.02 4.1364E-03 8.2351E-08 49297 50561 102.6% " 1,264 " 18 13 18 13 10 9 22 9 17 14 18 23 10 15 16 16 20 11 15 23 10 18 4 7 12 LP/J original M 1 1 091394D "59,057" "±4,842" "54,728" "±6,817" 12.5% "46,646" 117.33 1.345 JR000676 ? 940401 940914 166 28.0 360 0 1 "Great majority of unmyelinated fibers made it hard to count. Date perfused listed as Sept 14, 1994, but case referred to as 091394D." none Oct-16-94 9 "Box 29, Slot S-2" 2 "13,026 x" 193 x DR RW and RW "1,745 mm^2" "5,418 mm^2" 31.9 µm^2 0.89% 1461 13 487 37.5 ±16.16 ±4.67 2.7289E-03 4.6208E-08 49263 64878 131.7% " 15,615 " 54 26 27 22 36 20 61 30 19 40 66 54 32 LP/J replicate 0.00001 091394D "63,465" "±6,879" 10.8% "48,536" 130.76 "Repeat analysis of the same thin section. Good fixation, but again lots of very large unmyelinated fibers. Must be section close to lamina cribrosa." Mar-29-95 9 "Box 29, Slot S-2" "16,250 x" 191 x DR RW and RW "1,763 mm^2" "5,270 mm^2" 20.0 µm^2 0.86% 2432 21 548 26.1 ±12.65 ±2.83 37 27 6 37 5 33 46 32 14 24 33 21 24 52 23 25 21 19 10 16 double exposure 43 LP/J F 1 1 091394E "46,200" "±2,603" "46,200" "±2,603" 5.6% "66,684" 69.28 -1.186 JR000676 ? 940401 940914 166 26.0 330 1 1 "Date perfused listed as Sept 14, 1994, but case referred to as 091394E." none Oct-16-94 12 "Box 29, Slot T-1" 3 "13,024 x" 193 x DR RW and RW "2,489 mm^2" "5,418 mm^2" 31.9 µm^2 1.10% 2088 23 509 22.1 ±5.85 ±1.25 7.1271E-03 1.5427E-07 44515 47577 106.9% " 3,062 " 27 22 22 25 26 25 26 31 26 28 19 23 28 16 26 6 19 20 18 15 25 24 12 LP/J F 1 1 091394F "58,818" "±3,255" "58,818" "±3,255" 5.5% "89,071" 66.04 1.298 JR000676 ? 940401 940914 166 20.0 370 1 1 "Date perfused listed as Sept 14, 1994, but case referred to as 091394F." Low calibration lower than expected. Double-checked by Richelle April 1995. Area of original within 1.5% of second measurement of the same thin section. none Oct-24-94 14 "Box 30, Slot K-2" 3 "13,138 x" 193 x DR RW and RW "3,328 mm^2" "5,418 mm^2" 31.4 µm^2 0.78% 2838 22 456 20.7 ±5.26 ±1.15 5.8145E-03 9.8856E-08 59911 59911 100.0% 27 19 13 26 26 16 20 30 28 21 26 17 21 27 13 21 19 19 22 18 13 14 MOLD/Rk Get brain weight M 1 4 910507D "43,758" "±4,207" "±8,414" 5 "52,400" "±3,235" "52,400" "±3,235" 6.2% "62,506" 83.83 1.027 Dan Goldowitz colony #13 L 316 901019 910507 200 NA embedded 0 1 Older than 100 days M. molossiunus. Double checked by Rob "Many necrotic axons, large and small, about 1 per micrograph average" May-17-91 C4 "Box 13, Slot C-3" 3 "11,204 x" 373 x DT JO and JO and RW "8,689 mm^2" "5,292 mm^2" 42.2 µm^2 1.89% 1483 28 990 35.3 ±11.34 ±2.18 5.1928E-03 9.9101E-08 47656 57140 119.9% " 9,484 " 36 35 37 35 30 44 55 24 16 31 27 22 37 34 25 48 35 50 34 46 16 35 24 39 20 50 58 46.55 MOLD/Rk original F 1 4 910507E "32,348" "±2,241" "35,504" "±2,961" 8.3% "51,629" 68.77 Dan Goldowitz colony #16 L 901019 910507 200 NA embedded 1 Older than 100 days M. molossiunus. Unstained thin sections. Low contrast Many necrotic fibers. On average at least one per negative. May-17-91 D2 "Box 13, Slot M-2" "11,094 x" 373 x DT JO and JO and RW "7,166 mm^2" "5,292 mm^2" 43.0 µm^2 1.83% 1201 22 651 29.6 ±11.30 ±2.47 6.7465E-03 2.0856E-07 39514 31491 79.7% " 8,023 " 31.5 30 10 27 27 45 33 45 30 37 35 31 29 47 34 29 23 10 19 49 17 12 MOLD/Rk replicate 0.00001 910507E "32,066" "±3,430" 10.7% "51,754" 61.96 1 Very low contrast again. May have missed a few unmyelinated fibers. Necrotic fibers. Oct-26-94 D2 "Box 13, Slot M-2" "16,258 x" 192 x DR DR and RW "1,912 mm^2" "5,418 mm^2" 20.5 µm^2 0.79% 2525 20 254 12.7 ±5.92 ±1.36 33460 30441 91.0% " 3,019 " 18 22 7 21 13 7 12 20 6 6 13 12 13 20 10 11 19 0 10 14 MOLD/Rk replicate 2 0.00001 910507E "31,523" "±1,567" 5.0% "48,708" 64.72 Restained section and repeated again. Much better images. "Tons of necrotic fibers, 1-2 per micrograph" May-24-95 D2 "Box 13, Slot M-4" 3 "13,038 x" 247 x RW RW and RW "2,963 mm^2" "5,270 mm^2" 31.0 µm^2 2.04% 1571 32 642 20.1 ±5.55 ±1.00 18 15 22 23 17 21 20 19 8 23 18 15 18 24 21 17 25 23 25 12 14 34 25 15 14 17 20 23 24 16 22 34 MOLD/Rk original F 1 4 910507F "37,668" "±1,776" "40,295" "±2,480" 6.2% "52,617" 76.58 -0.724 Dan Goldowitz colony #17 R 901019 910507 200 NA embedded 1 1 Older than 100 days M. molossiunus. Brain weight data Many necrotic fibers. On average at least one per negative. May-17-91 D4 "Box 13, Slot I-1" 4 "11,159 x" 376 x DT JO and JO and RW "7,442 mm^2" "5,292 mm^2" 42.5 µm^2 1.78% 1238 22 716 32.5 ±9.18 ±2.00 1.2508E-02 3.3207E-07 42207 38380 90.9% " 3,827 " 48 45 22 30 21 41 34 38 23 36 31 31 40 23 33 31 38 41 26 13 25 46 MOLD/Rk replicate 0.00001 910507F "34,901" "±2,545" 7.3% "54,340" 64.23 Restained. Replicate by Rob May-24-95 D4 "Box 13, Slot I-1" "13,082 x" 247 x RW RW and RW "3,309 mm^2" "5,270 mm^2" 30.8 µm^2 1.02% 1765 18 356 19.8 ±5.95 ±1.44 21 14 27 12 21 22 27 15 10 29 31 16 18 19 20 20 13 21 MOLD/Rk M 1 4 090894M "49,185" "±3,141" "49,185" "±3,141" 6.4% "72,691" 67.66 0.645 Bought from JAX ? 940719 940908 51 13.5 316 0 2 Agouti. From F70+4. Male 105 M. molossiunus. Low contrast negatives A few necrotic fibers Nov-21-94 8 "Box 20 , Slot J-2" 3 "12,943 x" 193 x THK RW and RW "2,701 mm^2" "5,418 mm^2" 32.3 µm^2 0.76% 2248 17 372 21.9 ±5.59 ±1.40 5.2985E-03 1.0773E-07 45816 51092 111.5% " 5,276 " 30 13 18 24 32 27 22 19 18 22 15 21 18 16 23 23 31 MOLD/Rk Check other block F 1 4 090894N "47,191" "±2,286" "47,191" "±2,286" 4.8% "64,607" "3,310" 541 73.04 0.408 ? 940719 940908 51 13.0 329 1 Agouti MOLD/Rk. From F70+4. Female 104. Tissue switched with BALB/c 090894O. Negatives still labeled 090894O. "M. molossinus. Brain weight data is definitely corrected. Block number 9 apparently switched with BALB/cJ, thus block 12 is probably this MOLD/Rk. Data from case labeled BALB/cJ 090894O entered in micrograph columns. Reexamine companion blocks." 3 necrotic Dec-2-94 12 "Box 20, Slot O-2" 3 "12,997 x" 193 x TK RW and RW "2,411 mm^2" "5,418 mm^2" 32.1 µm^2 1.04% 2014 21 492 23.4 ±5.08 ±1.13 9.4832E-03 2.0095E-07 45396 46059 101.5% 663 25 19 19 23 24 22 17 15 34 33 23 27 26 24 23 21 30 27 18 17 25 NZB/BinJ right or left M 1 1 041593E "61,135" "±1,226" "±2,742" 6 "62,570" "±2,150" "61,370" "±2,449" 4.0% "95,052" 64.56 0.523 JR000684 ? CHECK BRAIN NA 930415 200 38.0 see notes 0 1 Retired breeder. Brain weight recorded as 0.357 gm. "Beautiful tissue, very few unmyelinated fibers" 1 necrotic fiber Oct-21-93 L6 "Box 22, Slot K-2" 4 "12,926 x" 250 x TK TK and RW "5,952 mm^2" "5,396 mm^2" 32.3 µm^2 1.16% 2943 34 709 20.9 ±4.78 ±0.83 1.3940E-02 2.2280E-07 65461 68677 104.9% " 3,216 " 24 21 17 19 25 21 27 22 24 20 14 14 18 18 31 16 21 18 22 22 29 20 20 23 18 15 10 15 18 26 22 29 23 27 NZB/BinJ other side 041593E simple average of two nerves "66,607" "±4,493" 6.7% "94,057" 70.82 none Jul-20-95 C5 "Box 49, Slot M-2" "12,850 x" 192 x SS SS and RW "3,451 mm^2" "5,270 mm^2" 31.9 µm^2 0.68% 2947 20 452 22.6 ±6.64 ±1.52 27 23 23 36 19 29 24 29 5 23 15 24 15 23 18 27 19 30 23 20 NZB/BinJ original F 1 1 041593G simple average of two nerves "60,652" "±2,277" "55,295" "±3,021" 5.5% "102,013" 54.20 -0.176 JR000684 R or L NA 930415 200 36.0 see notes 1 1 Retired breeder. Brain weight recorded as 0.361 gm. "Beautiful tissue, very few unmyelinated fibers" 2 necrotic Oct-21-93 D6 "Box 22, Slot M-2" 4 "12,823 x" 247 x TK TK and RW "6,216 mm^2" "5,419 mm^2" 33.0 µm^2 1.16% 3096 36 643 17.9 ±5.77 ±0.98 1.2033E-02 1.9839E-07 54972 57546 104.7% " 2,574 " 17 14 18 7 9 24 14 16 15 16 25 28 12 21 27 21 14 18 20 13 21 15 15 14 18 23 30 8 26 20 17 14 10 16 20 27 NZB/BinJ replicate 0.00001 041593G "59,259" "±3,465" 5.8% "104,402" 56.76 R or L Repeat analysis of a different thin section. May-3-95 D6 "Box 22, Slot M-2" "10,754 x" 246 x RW RW and RW "6,302 mm^2" "5,270 mm^2" 45.6 µm^2 0.96% 2291 22 569 25.9 ±6.93 ±1.51 17 14 37 32 30 31 21 28 32 29 33 21 20 31 35 23 13 20 33 21 24 24 NZB/BinJ other side 041593G "64,297" "±4,444" 6.9% "95,755" 67.15 L or R Othe side by Stacie none Jul-19-95 D5 "Box 50, Slot L-1" "12,697 x" 192 x SS SS and RW "3,523 mm^2" "5,270 mm^2" 32.7 µm^2 0.68% 2929 20 439 22.0 ±6.61 ±1.52 26 29 5 20 26 23 21 23 16 32 20 27 28 24 32 15 17 23 17 15 NZB/BinJ original M 1 1 041593F "56,090" "±2,497" "56,090" "±2,497" 4.5% "93,188" 60.19 -1.840 JR000684 ? NA 930415 200 35.8 see notes 0 1 Retired breeder. Brain weight recorded as 0.363 gm. Good tissue none Oct-26-93 D2 "Box 22, Slot L-1" 4 "13,028 x" 249 x TK TK and RW "5,770 mm^2" "5,342 mm^2" 31.5 µm^2 1.25% 2961 37 701 18.9 ±5.06 ±0.84 9.2464E-03 1.6485E-07 57548 64171 111.5% " 6,622 " 51 to 78 entered as 1-28 20 20 20 21 29 18 19 9 12 24 25 20 23 shifted by mistake 21 31 24 19 22 21 20 16 13 8 16 23 12 14 20 18 21 17 20 15 21 19 21 9 NZB/BinJ please reshoot 0.00001 041593F NZB/BinJ M 1 1 112194G "62,481" "±5,079" "62,481" "±5,079" 8.1% "69,151" 90.36 0.491 JR000684 ? 941016 941121 36 19.3 470 0 2 non-agouti 0.4281 gm 2 necrotic (on 11 and 19) Jan-31-95 14 "Box 37, Slot H-2" 4 "13,092 x" 194 x RW RW and RW "2,597 mm^2" "5,418 mm^2" 31.6 µm^2 1.14% 2188 25 714 28.6 ±11.37 ±2.32 2.5233E-03 4.0385E-08 67271 58057 86.3% " 9,213 " 36 20 25 24 36 24 25 46 27 40 30 33 6 37 41 39 26 44 37 20 6 21 9 21 41 NZB/BinJ right or left F 1 1 112194H simple average of two nerves "61,047" "±3,077" "65,378" "±4,101" 6.3% "64,418" 101.49 -0.032 JR000684 R or L 941016 941121 36 16.8 460 1 2 non-agouti 0.4173 gm 1 necrotic on 44 Jan-31-95 16 "Box 37, Slot I-1" 4 "13,075 x" 194 x RW RW and RW "2,419 mm^2" "5,418 mm^2" 31.7 µm^2 1.18% 2032 24 772 32.2 ±9.68 ±2.02 6.7302E-03 1.1025E-07 60464 70287 116.2% " 9,823 " 35 32 35 28 34 32 24 34 22 38 2 52 36 35 32 38 36 28 36 36 30 15 45 37 NZB/BinJ other side 112194H "55,470" "±4,653" 8.4% "69,213" 80.14 L or R none Jul-24-95 "15,989 x" 245 x SS SS and RW "4,155 mm^2" "5,270 mm^2" 20.6 µm^2 0.69% 3357 23 380 16.5 ±6.50 ±1.39 15 4 17 18 19 23 22 23 18 20 19 7 17 21 11 13 5 20 25 11 27 19 6 NZB/BinJ F 1 1 112194I "63,967" "±3,889" "63,967" "±3,889" 6.1% "67,948" 94.14 1.033 JR000684 ? 941016 941121 36 19.5 482 1 2 non-agouti 0.4440 gm none Jan-31-95 18 "Box 37, Slot J-4" 4 "13,099 x" 194 x TK RW and RW "2,551 mm^2" "5,418 mm^2" 31.6 µm^2 1.02% 2152 22 654 29.7 ±8.28 ±1.81 4.4312E-03 6.9273E-08 68660 59271 86.3% " 9,389 " 28 34 31 25 17 14 42 30 37 20 34 34 36 30 32 37 33 25 22 15 33 45 NZW/LacJ F 1 1 041593A "64,253" "±1,444" "±2,501" 4 "62,889" "±3,559" "62,889" "±3,559" 5.7% "127,631" 49.27 -0.545 JR001058 ? NA 930415 200 35.8 see notes 1 1 Retired breeder. Brain weight recorded as 0.313 gm. Very few unmyelinated fibers 3-5 necrotic Oct-21-93 D3 "Box 22, Slot N-1" 3 "12,833 x" 248 x TK TK and RW "7,874 mm^2" "5,429 mm^2" 33.0 µm^2 1.16% 3871 45 731 16.2 ±6.10 ±0.92 5.0773E-03 8.0735E-08 70229 61036 86.9% " 9,192 " 20 21 7 17 6 16 13 14 21 22 17 15 23 8 8 22 26 22 24 17 17 16 12 25 8 33 11 10 13 9 12 14 26 18 17 19 19 15 17 23 8 12 7 15 16 NZW/LacJ M 1 1 041593C "64,240" "±3,567" "64,240" "±3,567" 5.6% "119,621" 53.70 -0.005 JR001058 ? NA 930415 200 36.5 see notes 0 1 Retired breeder. Brain weight recorded as 0.355 gm. Negatives are labeled case B but this is actually the male case C. none Oct-26-93 D6 "Box 22, Slot O-2" 3 "12,833 x" 248 x TK TK and RW "7,380 mm^2" "5,429 mm^2" 33.0 µm^2 1.21% 3628 44 779 17.7 ±6.45 ±0.98 5.1656E-03 8.0411E-08 71101 69121 97.2% " 1,980 " 6 9 18 9 20 15 22 24 18 23 30 23 17 12 18 21 19 17 22 22 18 19 29 29 11 16 15 4 7 17 27 13 10 25 26 14 10 28 18 11 17 15 17 18 NZW/LacJ M 1 1 041593D "62,117" "±3,112" "62,117" "±3,112" 5.0% "91,074" 68.21 -0.854 JR001058 ? NA 930415 200 34.8 see notes 0 1 Retired breeder. Brain weight recorded as 0.336 gm. none Oct-26-93 C2 "Box 22, Slot P-1" 4 "12,833 x" 248 x TK TK and RW "5,619 mm^2" "5,429 mm^2" 33.0 µm^2 1.27% 2763 35 787 22.5 ±6.57 ±1.13 6.6038E-03 1.0631E-07 65215 66643 102.2% " 1,428 " 13 17 9 31 21 16 27 29 27 33 35 28 29 20 28 27 28 23 28 23 17 27 12 16 27 17 26 16 24 23 21 19 24 13 13 NZW/LacJ F 1 1 112194A "67,765" "±5,307" "67,765" "±5,307" 7.8% "71,814" 94.36 1.405 JR001058 ? 941121 36 20.0 459 1 2 Albino. Note large difference in brain weight with other NZW/LacJ 0.4138 gm none Jan-27-95 2 "Box 37, Slot D-2" 4 "13,073 x" 194 x TK RW and RW "2,702 mm^2" "5,418 mm^2" 31.7 µm^2 1.02% 2265 23 688 29.9 ±10.99 ±2.34 2.5157E-03 3.7124E-08 58333 78052 133.8% " 19,719 " 22 20 37 22 31 29 21 27 7 39 20 55 26 36 36 47 28 45 34 33 34 13 26 PANCEVO/Ei F 1 4 052793B "64,300" "±1,316" "±2,943" 6 "62,348" "±6,410" "62,348" "±6,410" 10.3% "201,790" 30.90 -0.663 JR1384 ? 436 921108 930527 200 17.0 447 1 1 "Retired breeder, probably from about F15." "M. hortulanus = M. spicilegus from Pancevo, Yugoslavia" none Aug-31-93 B2 or B3 "Box 25.5, Slot P" 3 "10,799 x" 143 x RW RC and RW "4,155 mm^2" "5,410 mm^2" 46.4 µm^2 0.48% 4350 21 301 14.3 ±6.59 ±1.47 1.5932E-03 2.5553E-08 62636 62083 99.1% 554 8 7 10 13 5 24 21 10 12 19 21 18 12 20 11 15 13 15 14 3 30 PANCEVO/Ei M 1 4 052793A "61,473" "±7,418" "61,473" "±7,418" 12.1% "89,590" 68.62 -0.960 JR1384 ? NA 930527 200 18.0 446 0 1 "Retired breeder, probably from about F15." "M. hortulanus = M. spicilegus from Pancevo, Yugoslavia" none Aug-31-93 B1 "Box 25.5, Slot O-2" 3 "13,164 x" 193 x RW RW and RW "3,352 mm^2" "5,410 mm^2" 31.2 µm^2 0.66% 2870 19 407 21.4 ±10.97 ±2.58 1.1793E-03 1.9185E-08 52292 67724 129.5% " 15,432 " 27 13 14 7 17 10 33 18 18 8 32 12 22 37 11 18 32 42 36 PANCEVO/Ei M 1 4 012194A "67,279" "±5,821" "67,279" "±5,821" 8.7% "111,959" 60.09 1.012 JR1384 ? 931216 940121 36 11.0 420 0 2 Lovely tissue. Negatives are mislabeled CB6F1 "M. hortulanus = M. spicilegus from Pancevo, Yugoslavia. Agouti coat" none Mar-24-94 9 "Box 25, Slot L-1" 4 "12,979 x" 247 x TK TK and RW "6,813 mm^2" "5,472 mm^2" 32.5 µm^2 0.84% 3447 29 566 19.5 ±8.93 ±1.69 2.0568E-03 3.0571E-08 67103 67464 100.5% 361 6 18 17 8 30 25 11 22 23 21 7 11 25 33 21 24 33 8 16 36 16 16 17 17 37 29 7 15 17 PANCEVO/Ei F 1 4 012194B "65,681" "±4,473" "65,681" "±4,473" 6.8% "66,766" 98.37 0.469 JR1384 ? 931216 940121 36 9.5 422 1 2 "Tissue from blocks 11 and 13 got switched. This is the PANCEVO/Ei, not the PLSJF1." Agouti coat Mar-25-94 11 "Box 25, Slot N-1" 4 "13,001 x" 248 x TK TK and RW "4,120 mm^2" "5,441 mm^2" 32.2 µm^2 1.01% 2074 21 665 31.7 ±9.65 ±2.16 3.4462E-03 5.2469E-08 70007 65639 93.8% " 4,368 " 38 41 19 39 33 32 25 41 26 26 34 34 32 17 40 10 31 38 37 51 21 PANCEVO/Ei M 1 4 020795B "67,721" "±5,327" "67,721" "±5,327" 7.9% "115,252" 58.76 1.162 ? 940922 950207 138 12.5 439 0 3 "Listed at CZECHII, but actually a PANCEVO/Ei" none May-17-95 3 "Box 44, Slot G-1" 4 "13,001 x" 193 x RW RW and RW "4,313 mm^2" "5,270 mm^2" 31.2 µm^2 0.76% 3696 28 513 18.3 ±7.49 ±1.44 2.4749E-03 3.6546E-08 29 21 14 16 17 11 17 18 5 13 17 22 12 31 21 16 19 31 12 20 13 7 22 7 31 32 23 16 PANCEVO/Ei M 1 4 020795C "61,296" "±3,378" "61,296" "±3,378" 5.5% "71,116" 86.19 -1.021 ? 940922 950207 138 13.5 444 0 3 "Listed at CZECHII, but actually PANCEVO/Ei" none May-17-95 5 "Box 44, Slot H-2" 3 "13,028 x" 194 x RW RW and RW "2,665 mm^2" "5,270 mm^2" 31.0 µm^2 0.92% 2290 21 562 26.8 ±6.59 ±1.47 5.6419E-03 9.2044E-08 51 and 52 entered as 1 and 2 36 40 25 27 23 27 12 17 27 34 26 25 16 26 27 33 24 32 28 25 32 PL/J M 1 1 041493A "55,976" "±1,263" "±3,341" 8 "60,287" "±3,072" "60,287" "±3,072" 5.1% "61,820" 97.52 1.290 JR000680 ? 446 930223 930414 50 21.3 see note 0 1 rd/rd and albino with kinked tail. Brain weight mismeasured at 0.267. 6 to 7 week old animal from JAX. Negatives light none Oct-14-93 B5 "Box 22, Slot G-1" 3 "12,848 x" 248 x TK TK and RW "3,790 mm^2" "5,318 mm^2" 32.2 µm^2 1.25% 1919 24 754 31.4 ±7.68 ±1.60 6.6654E-03 1.1056E-07 67593 67593 100.0% 15 26 38 36 21 41 45 32 41 28 28 37 29 17 27 34 36 33 23 27 35 37 39 29 PL/J original M 1 1 041493B simple average of two nerves "57,194" "±1,835" "63,723" "±3,390" 5.3% "62,129" 102.56 0.365 JR000680 ? 930223 930414 50 19.5 see note 0 1 rd/rd and albino with kinked tail. Brain weight mismeasured at 0.267 Negatives not enough contrast. Good count anyway. 6 to 7 week old animal from JAX. none Oct-14-93 C2 "Box 22, Slot H-2" 3 "12,892 x" 247 x TK TK and RW "3,790 mm^2" "5,376 mm^2" 32.3 µm^2 1.20% 1921 23 763 33.2 ±8.28 ±1.76 1.7748E-02 3.1032E-07 70993 68821 96.9% " 2,173 " 35 22 33 27 38 19 29 36 43 34 32 34 40 37 37 41 37 28 10 41 44 40 26 PL/J replicate 0.00001 041493B "62,678" "±3,379" 5.4% "63,238" 99.11 "Repeat on different thin section. Gorgeous tissue, suiable for framing." 1 necrotic on 28 Mar-29-95 C2 "Box 22, Slot H-2" "13,097 x" 191 x RW RW and RW "2,304 mm^2" "5,270 mm^2" 30.7 µm^2 0.97% 2058 20 609 30.5 ±7.16 ±1.64 36 27 26 26 14 29 32 42 38 28 34 25 25 30 34 34 30 47 24 28 PL/J other side 041493B "51,190" "±2,722" 5.3% "61,880" 82.72 Jul-20-95 C3 "Box 49, Slot K-2" "12,401 x" 246 x SS SS and RW "3,735 mm^2" "5,270 mm^2" 34.3 µm^2 1.11% 1806 20 567 28.4 ±6.57 ±1.51 23 27 26 17 25 32 16 31 23 34 26 37 37 22 29 25 31 36 30 40 PL/J M 1 1 041393A simple average of two nerves "56,216" "±2,101" "55,262" "±2,616" 4.7% "62,664" 88.19 0.072 JR000680 R or L 930223 930414 50 see note 0 1 rd/rd albino without kinked tail. Brain weight recorded as 0.277 gm. Great negatives. 6 to 7 week old animal from JAX. none Nov-4-93 A2 "Box 23, Slot M-2" 4 "12,994 x" 250 x TK TK and RW "3,904 mm^2" "5,353 mm^2" 31.7 µm^2 1.21% 1977 24 671 28.0 ±6.35 ±1.32 1.3291E-02 2.3643E-07 61315 56910 92.8% " 4,405 " 33 40 28 32 38 21 24 29 28 29 34 29 26 29 20 30 30 26 26 28 22 38 14 17 PL/J other side 041393A "57,947" "±3,524" 6.1% "47,342" 122.40 L or R Jul-24-95 "12,445 x" 246 x SS SS and RW "2,857 mm^2" "5,270 mm^2" 34.0 µm^2 1.44% 1391 20 833 41.7 ±11.04 ±2.53 39 44 39 44 37 36 50 48 blank 47 33 70 42 14 42 52 49 34 46 40 27 PL/J F 1 1 112194M "56,818" "±4,203" "56,818" "±4,203" 7.4% "62,042" 91.58 0.252 JR000680 ? 941016 941121 36 16.5 466 1 2 albino with kinked tail. rd/rd No problem with count. Just a very small nerve. none Jan-27-95 5 "Box 37, Slot E-1" 4 "13,169 x" 194 x TK TK and RW "2,334 mm^2" "5,418 mm^2" 31.2 µm^2 0.91% 1986 18 515 28.6 ±8.73 ±2.12 3.4063E-03 5.9951E-08 51410 62222 121.0% " 10,812 " 26 23 19 28 40 24 36 21 12 30 47 30 31 17 31 31 40 29 PL/J F 1 1 112194N "54,647" "±3,636" "54,647" "±3,636" 6.7% "49,734" 109.88 -0.398 JR000680 ? 941016 941121 36 16.0 444 1 2 albino with rd/rd none Jan-27-95 6 "Box 37, Slot F-2" 4 "13,145 x" 194 x TK TK and RW "1,871 mm^2" "5,418 mm^2" 31.4 µm^2 1.39% 1586 22 758 34.5 ±10.50 ±2.29 4.3307E-03 7.9249E-08 49887 59403 119.1% " 9,516 " 31 58 31 37 32 51 27 34 28 39 20 31 32 45 25 33 38 48 46 22 36 14 PL/J original M 1 1 112194O simple average of two nerves "50,498" "±1,991" "52,746" "±3,066" 5.8% "51,808" 101.81 -1.640 JR000680 ? 941016 941121 36 18.0 426 0 2 albino with rd/rd none Jan-31-95 8 "Box 37, Slot G-1" 4 "13,048 x" 194 x TK RW and RW "1,944 mm^2" "5,418 mm^2" 31.8 µm^2 1.23% 1628 20 648 32.4 ±8.21 ±1.88 1.3415E-02 2.6566E-07 49129 56287 114.6% " 7,158 " 31 30 37 35 24 31 35 10 42 39 26 30 45 40 26 35 41 29 39 23 PL/J replicate 0.00001 112194O "49,355" "±3,624" 7.3% "52,530" 93.96 Replicate of different thin section none Mar-23-95 8 "Box 37, Slot G-3" "13,048 x" 193 x TK RW and RW "1,949 mm^2" "5,418 mm^2" 31.8 µm^2 1.21% 1651 20 598 29.9 ±9.57 ±2.20 15 24 31 27 48 39 34 19 41 35 22 20 19 26 23 28 35 46 41 25 PL/J other side 112194O "49,664" "±3,623" 7.3% "43,818" 113.34 Jul-24-95 9 "Box 49, Slot Q-2" "15,916 x" 245 x SS SS and RW "2,622 mm^2" "5,270 mm^2" 20.8 µm^2 0.90% 2106 19 448 23.6 ±7.30 ±1.72 17 22 14 28 28 18 20 19 17 23 18 40 27 41 24 29 23 21 19 PL/J F 1 1 080593A "59,645" "±4,439" "59,645" "±4,439" 7.4% "69,800" 85.45 1.098 JR000680 JAX 4066 ? 930715 930805 21 Not perfused here. 1 albino. "New mutation 1839. Bilaterally affected iris. Right eye swollen with hazy cornea, left eye iris is abnormal" none Feb-14-94 D3 "Box 24, Slot Q-2" 3 "13,096 x" 248 x TK TK and RW "4,306 mm^2" "5,447 mm^2" 31.8 µm^2 0.96% 2197 21 570 27.1 ±9.03 ±2.02 3.1777E-03 5.3276E-08 65825 65167 99.0% 658 26 26 21 19 25 38 40 29 32 9 25 24 44 28 31 34 24 28 26 35 6 PL/J M 1 1 080593B "52,503" "±3,423" "52,503" "±3,423" 6.5% "83,821" 62.64 -1.039 JR000680 ? 930602 930805 64 Not perfused here. 2 albino. May have been right nerve. This eye did not have pupillary light reflex. "Right eye has a bad iris, white, will not dilate. Left eye normal? Good count, negatives a little fuzzy" none Mar-3-94 D5 "Box 24, Slot S-2" 3 "12,952 x" 248 x TK TK and RW "5,165 mm^2" "5,470 mm^2" 32.6 µm^2 1.01% 2571 26 531 20.4 ±6.66 ±1.33 4.6590E-03 8.8737E-08 59047 49820 84.4% " 9,226 " 13 19 11 23 15 11 16 18 27 28 14 11 21 32 28 36 21 17 21 25 12 19 21 24 23 25 PLSJF1 original F 1 3 012194C "56,096" "±1,279" "±3,134" 7 "50,030" "±2,828" "49,989" "±3,987" 8.0% "81,711" 61.18 -1.936 100299 ? 438 931202 940121 50 18.0 449 1 2 Lovely tissue. Negatives mislabeled CB6F1. Blocks 11 and 13 got switched. This is thefemale PLSJF1 none Mar-24-94 13 "Box 25, Slot M-2" 4 "12,799 x" 247 x TK TK and RW "4,997 mm^2" "5,445 mm^2" 33.2 µm^2 0.98% 2458 24 488 20.3 ±7.78 ±1.62 6.5298E-03 1.3052E-07 48349 51626 106.8% " 3,278 " see to right 5 20 12 30 29 17 14 16 22 2 25 26 29 23 16 24 17 17 34 14 28 26 21 21 PLSJF1 replicate 0.00001 012194C "50,071" "±4,010" 8.0% "67,118" 74.60 Replicate by Toya on different thin section to affirm low count. none Mar-24-95 13 "Box 25, Slot M-2" "12,977 x" 194 x TK RW and RW "2,536 mm^2" "5,418 mm^2" 32.2 µm^2 0.96% 2086 20 480 24.0 ±8.38 ±1.92 26 24 12 28 25 18 35 29 30 12 36 21 39 19 19 11 26 17 35 18 PLSJF1 F 1 3 012194D "58,973" "±4,135" "58,973" "±4,135" 7.0% "70,411" 83.76 0.918 100299 ? 931202 940121 50 16.0 430 1 1 white F1 of Pl/J female x SJL/J male 1 necrotic fiber on 0015 Mar-25-94 15 "Box 25, Slot O-2" 3 "13,003 x" 194 x TK TK and RW "2,644 mm^2" "5,508 mm^2" 32.6 µm^2 0.97% 2161 21 573 27.3 ±8.56 ±1.91 3.6211E-03 6.1402E-08 60304 58174 96.5% " 2,130 " 43 23 31 28 17 21 14 37 24 34 31 18 11 29 32 22 22 28 39 38 31 PLSJF1 M 1 3 012194E "57,883" "±3,363" "57,883" "±3,363" 5.8% "65,886" 87.85 0.570 100299 ? 931202 940121 50 21.5 447 0 1 white F1 of Pl/J female x SJL/J male maybe 1 necrotic Mar-25-94 17 "Box 25, Slot P-1" 4 "12,986 x" 195 x TK TK and RW "2,505 mm^2" "5,432 mm^2" 32.2 µm^2 0.98% 2045 20 566 28.3 ±7.17 ±1.64 5.3860E-03 9.3049E-08 58440 55619 95.2% " 2,821 " 15 26 26 24 23 29 28 29 32 18 33 27 19 32 32 47 31 34 37 24 PLSJF1 M 1 3 012194F "57,469" "±2,452" "57,469" "±2,452" 4.3% "62,523" 91.92 0.438 100299 ? 931202 940121 50 21.3 439 0 1 white F1 of Pl/J female x SJL/J male Mar-25-94 19 "Box 25, Slot Q-2" 4 "12,960 x" 193 x TK TK and RW "2,329 mm^2" "5,436 mm^2" 32.4 µm^2 1.04% 1932 20 595 29.8 ±5.53 ±1.27 1.0060E-02 1.7505E-07 53741 61696 114.8% " 7,954 " 39 30 31 25 20 35 26 25 31 29 37 26 30 25 38 32 29 20 37 30 PLSJF1 M 1 3 013194A "58,182" "±2,888" "58,182" "±2,888" 5.0% "65,109" 89.36 0.666 100299 ? 931202 940131 60 23.0 447 0 1 white F1 of Pl/J female x SJL/J male none Jun-16-94 7 "Box 27, Slot F-2" 3 "12,946 x" 194 x TK TK and RW "2,448 mm^2" "5,411 mm^2" 32.3 µm^2 0.99% 2017 20 577 28.9 ±6.24 ±1.43 7.3406E-03 1.2616E-07 60212 62553 103.9% " 2,340 " 28 35 27 40 21 28 26 33 30 28 32 31 18 30 33 24 30 14 38 31 PLSJF1 original M 1 3 013194B "56,133" "±2,493" "51,034" "±3,269" 6.4% "67,278" 75.86 0.012 100299 ? 931202 940131 60 25.0 448 0 1 white F1 of Pl/J female x SJL/J male Jun-16-94 8 "Box 27, Slot G-1" 3 "13,015 x" 192 x TK TK and RW "2,489 mm^2" "5,404 mm^2" 31.9 µm^2 0.95% 2109 20 484 24.2 ±6.76 ±1.55 9.5051E-03 1.6933E-07 50890 54369 106.8% " 3,480 " 21 13 17 20 10 25 30 25 25 27 29 30 26 23 21 31 36 18 35 22 PLSJF1 replicate 0.00001 013194B "63,228" "±3,856" 6.1% "67,228" 94.05 May-15-95 8 "Box 27, Slot G-1" "13,062 x" 248 x RW EGW and RW "4,138 mm^2" "5,270 mm^2" 30.9 µm^2 0.92% 2177 20 581 29.1 ±7.72 ±1.77 25 12 25 30 32 21 25 35 49 36 19 37 32 32 26 35 28 27 28 27 PLSJF1 M 1 3 013194C "53,998" "±3,118" "53,998" "±3,118" 5.8% "64,881" 83.23 -0.669 100299 ? 931202 940131 60 22.5 414 0 1 white F1 of Pl/J female x SJL/J male 1 necrotic Jun-16-94 11 "Box 27, Slot H-2" 3 "12,794 x" 192 x TK TK and RW "2,381 mm^2" "5,435 mm^2" 33.2 µm^2 1.13% 1954 22 608 27.6 ±7.31 ±1.60 5.8185E-03 1.0775E-07 57184 61884 108.2% " 4,700 " 30 24 34 22 24 36 13 34 30 35 38 30 39 29 29 17 24 29 31 23 24 13 PLSJF1 "Please shoot Box 28, Slot K" 013194D 940131 432 dup white F1 of Pl/J female x SJL/J male "Box 28, Slot K" SJL/J F 1 1 012094G "52,473" "±1,684" "±3,766" 6 "51,788" "±3,018" "51,788" "±3,018" 5.8% "70,451" 73.51 -0.182 100299 ? 400 931201 940120 50 14.3 404 1 1 "All SJL/Js carry rd/rd mutation. Negatives are mislabeled 011994F on negative, but case is not BALB/cJ" "2-4 necrotic axons, negative 20?" Mar-22-94 4 "Box 25, Slot H-2" 4 "13,000 x" 248 x TK TK and RW "4,319 mm^2" "5,456 mm^2" 32.3 µm^2 1.19% 2182 26 617 23.7 ±6.91 ±1.38 5.9143E-03 1.1420E-07 54483 59857 109.9% " 5,374 " 21 24 7 21 25 34 20 19 14 28 19 18 29 30 dup 37 30 21 30 27 19 25 23 20 24 36 16 SJL/J M 1 1 012094H "52,418" "±2,857" "52,418" "±2,857" 5.5% "72,502" 72.30 -0.015 100299 ? 931201 940120 50 18.0 428 0 1 All SJL/Js carry rd/rd mutation. Mislabeled 011994E on negative but not BALB/cJ "2 necrotic on neg 39, 1 necrotic on 42" Mar-22-94 2 "Box 25, Slot I-1" 4 "13,000 x" 248 x TK TK and RW "4,445 mm^2" "5,448 mm^2" 32.2 µm^2 1.02% 2249 23 536 23.3 ±5.96 ±1.27 6.7138E-03 1.2808E-07 54266 57335 105.7% " 3,069 " 23 18 21 31 16 9 23 34 21 27 18 21 24 21 28 14 30 22 28 23 25 29 30 SJL/J F 1 1 112194J "53,921" "±2,712" "53,921" "±2,712" 5.0% "51,920" 103.85 0.384 JR000689 ? 941016 941121 36 14.3 376 1 4 Albino. All SJL/Js carry rd/rd mutation. 1 necrotic Jan-28-95 19 "Slot 37, Slot A-1" "13,118 x" 194 x TK RW and RW "1,954 mm^2" "5,418 mm^2" 31.5 µm^2 1.82% 1649 30 981 32.7 ±8.86 ±1.64 7.5859E-03 1.4068E-07 54085 53755 99.4% 330 32 43 27 39 30 38 36 38 31 37 34 46 45 14 32 28 36 31 32 35 26 38 44 32 40 30 38 18 6 25 SJL/J F 1 1 112194K "56,439" "±3,653" "56,439" "±3,653" 6.5% "62,574" 90.19 1.053 JR000689 ? 941016 941121 36 15.8 398 1 4 Albino. All SJL/Js carry rd/rd mutation. 2 necrotic Jan-28-95 21 "Box 37, Slot B-4" 4 "12,981 x" 194 x TK RW and RW "2,355 mm^2" "5,418 mm^2" 32.2 µm^2 1.23% 1946 24 696 29.0 ±9.00 ±1.88 4.4128E-03 7.8187E-08 59880 52368 87.5% " 7,512 " 16 28 22 49 29 29 36 11 35 25 26 not in nerve 25 19 29 33 39 29 24 23 49 39 28 25 28 SJL/J F 1 1 112194L "54,705" "±4,225" "54,705" "±4,225" 7.7% "50,214" 108.94 0.592 JR000689 ? 941016 941121 36 16.5 394 1 4 Albino. All SJL/Js carry rd/rd mutation. none Jan-28-95 3 "Box 37, Slot C-1" 4 "13,076 x" 194 x TK RW and RW "1,889 mm^2" "5,418 mm^2" 31.7 µm^2 1.33% 1585 21 725 34.5 ±11.92 ±2.67 3.2184E-03 5.8833E-08 51281 58468 114.0% " 7,188 " 31 36 48 46 47 34 43 44 23 41 37 51 17 28 50 35 12 33 35 21 13 SJL/J please reshoot M 1E-08 062092A poor focus "46,795" "±2,438" 5.2% "61,802" 75.72 "47,801 " JR000689 R 911204 920621 200 32.0 NA 14.5 3 "Carry retinal degeneration mutation. Retired breeder. Negatives out of focus, or drift during photography" 5-10 necrotic fibers in series Sep-17-92 B4 "Box 18?, Ask Denny, Slot J-1 or J-4" 2 "11,147 x" 202 x DR DR and RW "2,522 mm^2" "5,033 mm^2" 40.5 µm^2 1.77% 1526 27 828 30.7 ±8.15 ±1.60 44461 50739 114.1% " 6,279 " 28 30 42 16 off of nerve 39 30 25 38 33 31 31 26 34 17 38 36 24 31 13 36 32 34 44 34 20 43 23 SJL/J M 1 1 120292B "45,570" "±3,237" "45,570" "±3,237" 7.1% "55,349" 82.33 -1.833 "41,504 " JR000689 L 920916 921203 78 29.0 459 12.7 0 2 "Carry retinal degeneration mutation. Retired breeder. Negatives blurred in first session of Denny's due to drift. Listed as a male in Denny's injection notes. First estimate was 38,924 +/- 1,979. Area estimate was only 40,061 square microns." "No problems with this new count. Denny's original count was from block C6, Box 21, Slot C-3 (Dec 16, 1992). The current data set is from a repeat analysis of newly cut section by Rob." 3 or more necrotic Apr-19-95 4 "13,112 x" 246 x RW RW and RW "3,344 mm^2" "5,270 mm^2" 30.7 µm^2 1.16% 1806 21 530 25.2 ±8.02 ±1.79 4.5657E-03 1.0019E-07 30 34 35 29 24 13 30 22 21 33 22 9 28 18 34 9 25 26 26 25 37 SPRET/Ei F 1 4 012094I "59,049" "±1,804" "±4,035" 6 "64,447" "±3,616" "64,447" "±3,616" 5.6% "87,826" 73.38 1.338 JR1146 ? 332 931216 940120 35 9.5 356 1 1 mislabeled 011994G on negative but not C57BL/6J M. spretus from Spain 1 necrotic on neg 21 Mar-22-94 5 "Box 25, Slot J-2" 4 "13,017 x" 249 x TK TK and RW "5,432 mm^2" "5,448 mm^2" 32.2 µm^2 0.99% 2732 27 637 23.6 ±6.75 ±1.32 5.1186E-03 7.9423E-08 74385 55218 74.2% " 19,167 " 19 32 28 27 20 31 22 28 22 39 27 33 20 29 10 24 24 27 11 19 26 27 13 16 22 22 19 SPRET/Ei M 1 4 012094J "53,323" "±3,800" "53,323" "±3,800" 7.1% "79,406" 67.15 -1.419 JR1146 ? 931216 940120 35 11.3 350 0 1 wonderful fixation M. spretus from Spain none Mar-24-94 8 "Box 25, Slot K-2" 4 "12,961 x" 248 x TK TK and RW "4,893 mm^2" "5,474 mm^2" 32.6 µm^2 1.03% 2437 25 547 21.9 ±7.64 ±1.56 3.8460E-03 7.2126E-08 53817 52864 98.2% 953 25 19 36 20 25 24 24 30 18 21 19 28 30 27 35 23 25 18 15 10 13 21 1 24 16 SPRET/Ei please reshoot 1E-08 012094J SPRET/Ei M 1 4 042693D "58,243" "±3,191" "58,243" "±3,191" 5.5% "97,490" 59.74 -0.200 JR1146 ? 930407 930512 35 362 0 2 M. spretus from Spain none Jun-14-93 B6 "Box 25.5, Slot K-2" 3 "13,097 x" 195 x DR RW and RW "3,689 mm^2" "5,410 mm^2" 31.5 µm^2 1.23% 3091 38 716 18.8 ±6.28 ±1.03 5.8739E-03 1.0085E-07 56452 60031 106.3% " 3,579 " 20 13 20 21 12 11 24 22 13 13 34 26 10 19 22 16 3 13 23 22 20 18 17 15 29 23 30 26 20 12 17 25 14 17 18 12 23 23 SPRET/Ei original F 1 4 042693C "55,783" "±2,519" "54,624" "±2,746" 5.0% "91,164" 59.92 -0.810 JR1146 ? 930407 930512 35 336 1 2 "High mag recalibrated by Denny and Rob on Oct 26, 1994 by rephotographing one field at 12K nominal." M. spretus from Spain none Jun-14-93 B3 "Box 25.5, Slot L-3" 3 "13,809 x" 197 x DR RW and RW "3,535 mm^2" "5,410 mm^2" 28.4 µm^2 1.09% 3213 35 595 17.0 ±4.98 ±0.85 9.0474E-03 1.6219E-07 53111 56051 105.5% " 2,940 " 16 28 18 15 19 12 10 16 11 5 15 19 13 18 32 15 20 13 18 12 25 19 12 17 17 17 16 21 19 22 20 17 17 16 15 SPRET/Ei replicate 0.00001 042693C "61,959" "±6,338" 10.2% "89,652" 69.11 JR1146 ? 930512 Redone Oct 1994 by Rob. Initial low calibration of 173.2 was screwed up. Low power shots of this thin section retaken by Toya with new low calibration. M. spretus from Spain none Oct-24-94 B3 "Box 25.5, Slot L-3" "16,271 x" 194 x RW RW and RW "3,374 mm^2" "5,418 mm^2" 20.5 µm^2 0.32% 4381 14 198 14.1 ±5.22 ±1.45 48891 55200 112.9% " 6,309 " 24 18 12 17 17 10 18 11 14 3 11 16 9 18 SPRET/Ei F 1 4 091494B "57,579" "±3,547" "57,579" "±3,547" 6.2% "44,432" 129.59 -0.364 JR1146 ? 940815 940914 30 8.0 300 1 3 Tissue quality is OK. May also be listed as 091394B. JAX order number 785003. M. spretus from Spain none Oct-9-94 5 or 6 "Box 29, Slot I-3" 2 "12,968 x" 191 x DR DR and RW "1,621 mm^2" "5,418 mm^2" 32.2 µm^2 1.45% 1379 20 835 41.8 ±11.21 ±2.57 4.8177E-03 8.3672E-08 57095 58060 101.7% 965 46 47 39 9 44 51 56 40 26 34 38 48 39 41 50 51 48 59 35 34 SPRET/Ei original F 1 4 091494A "61,808" "±2,139" "63,171" "±3,101" 4.9% "46,136" 136.92 0.684 JR1146 2938 ? 940816 940914 29 12.0 310 1 4 F38. May also be listed as 091394A. M. spretus from Spain none Oct-12-94 4 "Box 29, Slot H-2" 3 "16,052 x" 190 x RW RW and RW "1,671 mm^2" "5,418 mm^2" 21.0 µm^2 0.87% 2194 19 547 28.8 ±6.00 ±1.41 1.4255E-02 2.3064E-07 60949 65167 106.9% " 4,218 " 27 23 36 25 21 39 36 27 26 28 35 40 31 23 24 21 31 24 30 SPRET/Ei replicate F 0.00001 4 091494A "60,570" "±2,955" 4.9% "45,651" 132.68 M. spretus from Spain Replicate of different thin section. By error thought to be a BXD9 (H-2 confused with M-2). All negatives relabeled. none Mar-12-95 4 "Box 29, Slot H-2" "13,138 x" 193 x RW RW and RCS "1,696 mm^2" "5,418 mm^2" 31.4 µm^2 1.38% 1454 20 833 41.7 ±8.86 ±2.03 40 38 46 30 25 44 30 33 51 55 60 47 47 47 40 38 42 48 33 39 TgN(Hbb-b1)83/LoGo replicate M 1 9 910311D "56,912" "±1,969" "±4,404" 6 "57,994" "±2,291" "62,941" "±3,342" 5.3% "79,746" 78.93 0.246 Dan Goldowitz colony R NA 910311 100 0 1 An SJL/L by C57BL/6J partially inbred (10-15 full sib matings) transgenic mouse. "Stud mouse 330. First count by Daphne was only 41,724. Some error, most in count from sampling micrographs and poor calibration." None Oct-26-94 C2 "Box 11, Slot M-2" 4 "13,118 x" 192 x DR DR ad RW "2,940 mm^2" "5,418 mm^2" 31.5 µm^2 0.79% 2533 20 497 24.9 ±5.75 ±1.32 1.1628E-02 2.0051E-07 66865 59013 88.3% " 7,852 " 16 27 30 24 18 20 14 27 32 33 22 28 30 31 32 26 17 23 22 25 TgN(Hbb-b1)83/LoGo original 0.00001 910311D "53,607" "±3,147" 5.9% "72,491" 73.95 Daphne's oiginal. The high mag calibration is from a torn region and is way off. Apr-10-91 C2 "Box 11, Slot M-2" "11,300 x" 361 x DT DT and DT "9,466 mm^2" "5,366 mm^2" 42.0 µm^2 1.57% 1725 27 839 31.1 ±9.30 ±1.82 25 21 22 41 28 19 26 40 39 47 42 35 24 26 32 30 30 38 37 39 31 11 49 31 20 36 20 TgN(Hbb-b1)83/LoGo M 1 9 910311E "59,295" "±2,517" "59,295" "±2,517" 4.2% "95,229" 62.27 0.541 Dan Goldowitz colony R NA 910311 100 0 1 All calibrations redone by Rob. Known in lab as GTO strain. An SJL/L by C57BL6J partially inbred transgenic mouse. Stud mouse 331. Get age from GTO book May-2-91 C6 "Box 12, Slot K-4" 3 "11,088 x" 393 x DT DT and DT and RW "14,708 mm^2" "5,366 mm^2" 43.6 µm^2 1.83% 2182 40 1087 27.2 ±7.20 ±1.15 9.6003E-03 1.6191E-07 59460 60545 101.8% " 1,084 " 17 25 20 27 28 29 39 26 15 19 25 edge 28 27 39 18 29 30 45 28 26 19 24 22 22 25 41 36 28 42 34 34 29 30 23 24 20 27 24 27 16 TgN(Hbb-b1)83/LoGo original F 1 9 900208.3 "62,615" "±1,897" "64,987" "±2,593" 4.0% "52,228" 124.43 1.295 Dan Goldowitz colony L mid orbital NA 900208 100 1 2 An SJL/L by C57BL/6J partially inbred (10-15 full sib matings) transgenic mouse. "Fine, good tissue" 1 necrotic May-2-91 A3 "Box 11, Slot K-2" 3 "11,300 x" 386 x DT DT and RW "7,801 mm^2" "5,366 mm^2" 42.0 µm^2 1.69% 1243 21 1098 52.3 ±9.33 ±2.09 1.8276E-02 2.9188E-07 64860 68385 105.4% " 3,525 " 40 70 63 62 53 50 47 55 65 49 58 41 47 41 46 50 43 64 58 58 38 TgN(Hbb-b1)83/LoGo replicate 0.00001 900208.3 "60,606" "±2,782" 4.6% "68,300" 88.73 L near chiasm dup May-9-91 A4 "Box 12, Slot O-2" "11,094 x" 375 x DT DT and RW "9,619 mm^2" "5,366 mm^2" 43.6 µm^2 2.23% 1567 35 1354 38.7 ±10.35 ±1.78 59371 62787 105.8% " 3,416 " 40 29 31 35 50 37 43 40 30 17 42 11 41 37 25 41 40 55 32 38 47 59 56 35 42 48 39 41 40 50 30 22 46 39 46 TgN(Hbb-b1)83/LoGo replicate 2 0.00001 900208.3 "60,178" "±5,108" 8.5% "77,409" 77.74 ? dup "Terrible Formvar. Photographed at 20,000X. Accurate count. No problem in counting." Oct-24-94 A2 "Box 2, Slot K-2" "21,544 x" 174 x RW RW and RW "2,336 mm^2" "5,250 mm^2" 11.3 µm^2 0.42% 6844 29 255 8.8 ±3.95 ±0.75 58659 61592 105.0% " 2,933 " 16 12 8 9 11 16 9 1 11 11 10 12 9 0 8 15 6 4 12 12 8 7 9 8 8 8 8 5 2 TgN(Hbb-b1)83/LoGo F 1 9 231 "58,163" "±4,812" "58,163" "±4,812" 8.3% "63,809" 91.15 0.284 Dan Goldowitz colony 231 R NA NA 100 1 An SJL/L by C57BL/6J partially inbred (10-15 full sib matings) transgenic mouse. "First count of 48,000 screwed up, apparently due to miscalibartion of montage (area or 51, 776 microns squared) or low mag of sample (x7,500). " 1 necrotic on 0002 Dec-23-94 B6 "Box 4, Slot H-4" 4 "16,122 x" 193 x THK THK and RW "2,381 mm^2" "5,418 mm^2" 20.8 µm^2 0.52% 3061 16 304 19.0 ±6.09 ±1.57 2.6791E-03 4.6063E-08 61222 55100 90.0% " 6,122 " 23 21 20 23 26 17 1 23 18 18 22 24 11 16 23 18 TgN(Hbb-b1)83/LoGo F 1 9 900927B "52,646" "±2,528" "52,646" "±2,528" 4.8% "61,532" 85.56 -0.969 289 L NA 900927 100 1 3 Feb-13-91 B4 "Box 10, Slot I-1" 4 "11,010 x" 389 x DT DT and DT "9,311 mm^2" "5,292 mm^2" 43.7 µm^2 2.13% 1410 30 1121 37.4 ±9.66 ±1.79 8.5239E-03 1.6191E-07 50804 57437 113.1% " 6,633 " 37 36 35 18 50 45 29 17 27 42 27 48 37 43 30 47 44 36 44 33 57 28 52 28 37 39 33.5 31 49 41 TgN(Hbb-b1)83/LoGo F 1 9 900927A "50,762" "±2,917" "49,217" "±3,911" 7.9% "51,876" 94.87 -1.397 Dan Goldowitz colony 294 L NA 900927 100 1 3 An SJL/L by C57BL/6J partially inbred (10-15 full sib matings) transgenic mouse. triple checked calibrations Feb-13-91 B2 "Box 10, Slot J-2" 4 "11,178 x" 389 x DT DT and DT and RW "7,850 mm^2" "5,250 mm^2" 42.0 µm^2 1.78% 1235 22 877 39.9 ±14.52 ±3.17 6.2478E-03 1.2308E-07 46916 48712 103.8% " 1,796 " 13 38 39 42 68 29 47 28 54 48 30 57 17 40 37 51 54 56 38 14 30 47 TgN(Hbb-b1)83/LoGo 0.00001 900927A "52,700" "±4,381" 8.3% "54,789" 96.19 ? dup none Dec-23-94 B2 "Box 10, Slot J-2" "16,122 x" 193 x THK THK and RW "2,044 mm^2" "5,418 mm^2" 20.8 µm^2 0.76% 2628 20 401 20.1 ±7.27 ±1.67 51779 53619 103.6% " 1,840 " 23 7 34 25 19 23 28 32 8 19 20 29 14 18 20 17 19 10 19 17 TgR(ROSA26)26Sor<Ñ>BALB/c Left M 121594A "56,741" "±4,089" "56,741" "±4,089" 7.2% "71,053" 79.86 Left 941018 941215 58 29.7 496 19.7 1 "HRP injected into right thalamus, but backfill in right retina not perfect. Only 810 cells labeled. Approximate ROSA26 genotype in VTC is 20%." Fixed with fresh 4% paraformaldehyde by Denny. Brain taken by Dan and not weighed. PRetty good for paraformaldehyde tissue. 1 necrotic? May-15-95 19 "Box 44, Slot C-1" 3 "13,100 x" 194 x DR RW and RW "2,674 mm^2" "5,270 mm^2" 30.7 µm^2 0.91% 2314 21 515 24.5 ±7.90 ±1.77 3.5635E-03 6.2803E-08 24 24 22 19 27 26 4 27 28 20 22 29 23 18 double exposure 30 27 20 34 14 42 35 TgR(ROSA26)26Sor<Ñ>BALB/c Right 121594A 18.5 TgR(ROSA26)26Sor<Ñ>BALB/c Right M 121594B "63,731" "±3,789" "63,731" "±3,789" 5.9% "80,906" 78.77 "1,234 " Right 941018 941215 58 28.7 459 17.6 1 "HRP injected into right thalamus of this chimera. Total count of uncrossed RGCs in the right retina is 1,234. 1.96% uncrossed. Approximate ROSA26 genotype in VTC is also 20%." Fixed with fresh 4% paraformaldehyde by Denny. Brain taken by Dan and not weighed. none May-15-95 20 "Box 44, Slot D-2" 3 "13,100 x" 248 x DR DR and DR and RW "4,980 mm^2" "5,270 mm^2" 30.7 µm^2 0.80% 2635 21 508 24.2 ±6.43 ±1.44 4.6619E-03 7.3149E-08 27 19 37 25 20 23 21 26 18 22 38 25 29 11 22 26 15 21 31 28 24 TgR(ROSA26)26Sor<Ñ>BALB/c Left 121594B 22.2 TgR(ROSA26)26Sor<Ñ>BALB/c Right F 011895B 941122 950118 57 26.0 2 "HRP injected into right thalamus of this chimera. About 1,600 ipsilateral cells in large BALB/c dominated VTC." 9=RON TgR(ROSA26)26Sor<Ñ>BALB/c Left 011895B 8=LON TgR(ROSA26)26Sor<Ñ>BALB/c M 012395A 941122 950118 57 25.5 2 "HRP injected into right thalamus of this chimera. About 1,600 ipsilateral cells in large BALB/c dominated VTC." TgR(ROSA26)26Sor<Ñ>BALB/c 012395A TgR(ROSA26)26Sor<Ñ>BALB/c M 012395B 941122 950118 57 25.9 2 HRP injected into right thalamus of this chimera. TgR(ROSA26)26Sor<Ñ>BALB/c 012395B TgR(ROSA26)26Sor<Ñ>BALB/c Right M 051995C 3 HRP injected into right thalamus of this chimera. Retina is predominantly ROSA26 genotype. 19=RON TgR(ROSA26)26Sor<Ñ>BALB/c Left 051995C 18=LON TgR(ROSA26)26Sor<Ñ>BALB/c Right F 051995D 3 HRP injected into right thalamus of this chimera. Retina is predominantly ROSA26 genotype. 21=RON TgR(ROSA26)26Sor<Ñ>BALB/c Left 051995D 20=LON TgR(ROSA26)26Sor<Ñ>BALB/c Right F 051995E 3 HRP injected into right thalamus of this chimera. Retina is predominantly ROSA26 genotype. 1=RON TgR(ROSA26)26Sor<Ñ>BALB/c Left 051995E 2=LON TgR(ROSA26)26Sor M 051995A 950315 950519 65 19.7 Not a chimera. No data on brain weight. Optic tract injected with HRP. Get retina data. 14 or 15 TgR(ROSA26)26Sor F 051995B 950315 950519 65 15.6 Not a chimera. No data on brain weight. Optic tract injected with HRP. Get retina data. 16 or 17 TgR(ROSA26)26Sor M 071095P 950710 100 26.0 427 Not a chimera. TgR(ROSA26)26Sor M 071095Q 950710 100 27.0 427 Not a chimera. TgR(ROSA26)26Sor M 071195A 950711 100 25.0 430 Not a chimera TgR(ROSA26)26Sor F 071195B 950711 100 27.0 512 Not a chimera TgR(ROSA26)26Sor F 071195C 950711 100 29.5 504 Not a chimera TgR(ROSA26)26Sor F 071195D 950711 100 32.0 496 Not a chimera WSB/Ei M 1 4 052793E "57,380" "±1,698" "±4,492" 8 "58,568" "±4,665" "58,568" "±4,665" 8.0% "132,347" 44.25 0.265 JR1145 ? 423 051108 060527 200 21.0 463 0 1 Mus domesticus (WSB from Maryland). White spot blaze on agouti. Retired breeder 1 nec on 0033? Aug-31-93 C6 "Box 25.5, Slot Q-4" 3 "13,138 x" 194 x RW RW and RW "4,959 mm^2" "5,410 mm^2" 31.3 µm^2 0.54% 4223 23 319 13.9 ±5.18 ±1.10 2.6916E-03 4.4947E-08 53132 62102 116.9% " 8,970 " 14 21 6 18 13 19 11 10 15 12 21 14 4 11 14 13 21 10 4 15 18 22 13 WSB/Ei F 1 4 052793F "63,021" "±2,902" "63,021" "±2,902" 4.6% "118,935" 52.99 1.256 JR1145 ? 051108 060527 200 20.0 436 1 1 Mus domesticus (WSB from Maryland). White spot blaze on agouti. Retired breeder. High mag calibration negative given to Richard. Aug-31-93 D4 "Box 25.5, Slot R-1" 2 "12,891 x" 193 x RW RW and RW "4,450 mm^2" "5,410 mm^2" 32.6 µm^2 0.55% 3653 20 345 17.3 ±3.46 ±0.79 7.4842E-03 1.1575E-07 66766 61917 92.7% " 4,849 " 15 18 22 19 20 13 16 16 11 15 25 20 20 19 21 15 14 14 15 17 WSB/Ei F 1 4 072994G-2 "51,246" "±4,062" "51,246" "±4,062" 7.9% "78,230" 65.51 -1.366 JR1145 ? 940420 940729 100 14.5 421 1 2 White spot blaze on agouti. Mislabeled CASA/Rk due to Nash Facility screw up. This is actually a WSB/Ei strain none Nov-23-94 13 "Box 20, Slot H-2" 4 "12,944 x" 193 x THK RW and RW "2,903 mm^2" "5,418 mm^2" 32.3 µm^2 0.91% 2419 22 466 21.2 ±7.69 ±1.68 3.1056E-03 5.9208E-08 54593 49670 91.0% " 4,922 " 19 19 24 23 19 33 27 33 30 31 17 18 9 18 29 28 17 5 20 22 11 14 WSB/Ei F 1 4 072994F-2 "59,140" "±5,133" "59,140" "±5,133" 8.7% "118,770" 49.79 0.392 JR1145 ? 940420 940729 100 14.3 423 1 2 White spot blaze. Agouti mouse. Mislabeled CASA/Rk due to Nash Facility screw up. This is actually a WSB/Ei strain none Nov-23-94 12 "Box 20, Slot I-1" 2 "13,002 x" 194 x THK RW and RW "4,450 mm^2" "5,418 mm^2" 32.0 µm^2 0.65% 3706 24 383 16.0 ±6.64 ±1.39 2.2444E-03 3.7151E-08 14 10 20 14 8 15 20 9 12 17 15 32 27 29 17 13 25 9 6 16 14 14 14 13 WSB/Ei M 1 4 020795D "62,277" "±3,866" "62,277" "±3,866" 6.2% "94,205" 66.11 1.090 JR1145 ? 941207 950207 62 15.5 405 0 3 none May-17-95 8 "Box 44, Slot I-1" 4 "13,017 x" 193 x RCS RW and RW "3,515 mm^2" "5,270 mm^2" 31.1 µm^2 0.83% 3029 25 514 20.6 ±6.25 ±1.28 4.1674E-03 6.5565E-08 21 12 22 19 17 16 27 21 30 20 24 8 21 24 20 23 21 15 11 11 28 17 26 33 27 WSB/Ei M 1 4 020795E "50,826" "±2,699" "50,826" "±2,699" 5.3% "49,479" 102.72 -1.459 JR1145 ? 941207 950207 62 15.8 426 0 3 none May-18-95 10 "Box 44, Slot J-2" 4 "12,943 x" 247 x RCS RW and RW "3,026 mm^2" "5,270 mm^2" 31.5 µm^2 1.21% 1573 19 614 32.3 ±7.28 ±1.72 6.9774E-03 1.3362E-07 34 50 40 31 29 26 29 41 29 41 31 29 26 28 41 32 31 19 27 WSB/Ei F 1 4 020795F "56,875" "±3,322" "56,875" "±3,322" 5.8% "82,719" 68.76 -0.112 JR1145 ? 941207 950207 62 12.3 402 1 3 none May-18-95 GET "Box 44, Slot K-1" 4 "12,989 x" 194 x RCS RW and RW "3,113 mm^2" "5,270 mm^2" 31.2 µm^2 0.79% 2648 21 451 21.5 ±5.61 ±1.25 5.1551E-03 8.8456E-08 30 24 25 29 20 26 22 19 23 25 22 23 26 16 5 21 20 11 21 21 22 WSB/Ei F 1 4 020795G "57,086" "±5,031" "57,086" "±5,031" 8.8% "63,467" 89.95 -0.065 JR1145 ? 941207 950207 62 13.5 407 1 3 none May-17-95 13 "Box 44, Slot L-1" 4 "12,998 x" 248 x RW RW and RW "3,911 mm^2" "5,270 mm^2" 31.2 µm^2 0.88% 2035 18 505 28.1 ±10.19 ±2.47 2.2552E-03 3.8392E-08 40 34 26 29 30 27 12 26 11 20 30 36 21 29 33 35 52 14 AKXD28 M 072194B "59,524" "±3,585" "59,524" "±3,585" 6.0% "95,693" 62.20 ? 940420 940721 92 464 1 This AKXD RI line develops cataracts. one necrotic axon in #36 Oct-17-94 A1 "Box 29, Slot E-1 " "12,935 x" 194 x DR DR and RW "3,602 mm^2" "5,418 mm^2" 32.4 µm^2 0.71% 2955 21 423 20.1 ±5.42 ±1.21 4.6324E-03 7.5948E-08 57328 61518 107.3% " 4,191 " 15 10 22 9 27 23 19 21 22 30 24 15 16 15 20 19 24 25 19 27 21 AKXD28 M 072194A "64,435" "±4,740" "64,435" "±4,740" 7.4% "111,037" 58.03 ? 930704 940721 382 482 2 FN4F7 generation Oct-17-94 D6 "Box 29, Slot D-2 " "13,004 x" 194 x DR DR and RW "4,179 mm^2" "5,418 mm^2" 32.0 µm^2 0.78% 3466 27 502 18.6 ±6.97 ±1.37 2.8680E-03 4.3678E-08 65045 63865 98.2% " 1,181 " 25 14 17 26 27 27 6 14 28 15 12 23 13 28 27 17 16 17 19 13 13 24 22 2 21 24 12 AKXD28 M 072194C 921119 940721 609 523 3 "Right side cataract, pupil dilated. Left side partly dilated and also has a cataract."