Mouse Neuron Population Database Retinal Ganglion Cell Estimates "Version of Feb 2, 1996 (last version posted on WWW was Oct 10, 1995, n=450)" "Robert W. Williams, Richelle C. Strom, Dennis S. Rice, and Dan Goldowitz" Address questions and comments to rwilliam@nb.utmem.edu "With thanks to R. Cushing, S. Smith, B. Taylor, M. Davisson, and T. K. Hurt" number of independent samples 763 2460 number of individuals counted 573 "55,178" CV =SD/Mean "number cases, category 1" 540.00146 "2,449" 2.400 number cases included in first set of studies 451.00000 "Submitted Dec 13, 1995" number of types of mice 62 Dec 12 F2 Stats N = 54 cases total number of standard inbred strains 17 Criterion N mean SD SEM number of F1 cross types 5 ">65,000" 12 70331 "4,094" "1,234" number of BXD strains 26 "<50,000" 15 47082 "2,191" 585 number of BXH strains 12 number of wild inbred strains 7 number of non-inbred strains 3 number of N2 types 2 number of other strains 2 number of duplicate counts 146 number of R vs L nerve comparisons in study 19 "Multipliers of the range to get unbiased SD: n=2: x0.886, 3: 0.591, 4: 0.486, 5: 0.430, 6: .395, 7: 0.370, 8: 0.351, 9: 0.337, 10: 0.325, 11: 0.315, 12: 0.307, 13: 0.300, 14: 0.294, 15: 0.288, 16: 0.283, 17: 0.279, 18: 0.275, 19: 0.271, 20: 0.268 " average #DIV/0! ALL INBRED non-wild "t dist, 2-tailed, p=0.025 per tail)" See Table A6 of Snedecor and Cochran (1980) For Bartlett test of homogeneity of variance For Bartlett test of homogeneity of variance For Bartlett test of homogeneity of variance For Bartlett test of homogeneity of variance For Bartlett test of homogeneity of variance Strain or Species Neurons SEM SD CV N 95%CI- 95%CI+ Lowest Highest Range SD_Range Z Age Body Brain BrainRGC Corr Retina H_cells ±HC RGC/HC H or L type note Rank_RGC Z_Fract Z_Exp RGCs Rank_Brain Z_Fract_Brain RGC-Brain Correl Z_Exp_Brain Brain VAR LN VAR df df*LN(VAR) 1/df 129/J "63,772" "±1,699" "±4,163" 6.5% 7 "60,441" "67,103" "57,185" "68,409" "11,223" "±4,153" 0.40 76 20.0 407 16.5 ? inbred c/c 3 in prog 31 0.622 0.312 "63,772" 13 0.255 -0.674 -0.659 407 " 17,328,934 " 16.7 6 100 0.167 A/J "51,238" "±1,362" "±3,602" 7.0% 8 "48,569" "53,906" "46,312" "56,720" "10,408" "±3,653" -1.63 111 22.2 395 14.0 "7,873" ±114 6.5 L inbred done 1 0.010 -2.319 "51,238" 6 0.112 0.023 -1.215 395 " 12,976,709 " 16.4 7 115 0.143 AKR/J "62,788" ±890 "±1,990" 3.2% 6 "61,044" "64,532" "60,063" "65,143" "5,081" "±2,007" 0.24 71 - 449 14.7 "11,398" ±510 5.5 H inbred c/c done 26 0.520 0.051 "62,788" 37 0.745 0.210 0.659 449 " 3,959,370 " 15.2 5 76 0.200 BALB/cBy "57,558" "±1,768" "±4,330" 7.5% 7 "54,094" "61,023" "51,320" "63,924" "12,604" "±4,663" -0.61 30 14.4 403 - ? inbred c/c 3 in progress 17 0.337 -0.421 "57,558" 11 0.214 -0.629 -0.792 403 " 18,745,774 " 16.7 6 100 0.167 BALB/cJ "63,393" "±2,210" "±5,846" 9.2% 8 "59,062" "67,724" "53,252" "68,788" "15,536" "±5,453" 0.34 96 23.9 477 15.3 H inbred c/c done 28 0.561 0.154 "63,393" 46 0.929 0.208 1.465 477 " 34,174,206 " 17.3 7 121 0.143 BXD1 "61,996" "±1,077" "±3,231" 5.2% 10 "59,885" "64,107" "59,552" "67,167" "7,615" "±2,566" 0.11 51 20.5 448 22 0.439 -0.154 "61,996" 35 0.704 0.120 0.536 448 " 10,441,458 " 16.2 9 145 0.111 BXD2 "66,063" "±1,644" "±3,677" 5.6% 6 "62,840" "69,286" "60,417" "70,774" "10,358" "±4,091" 0.77 53 23.1 427 40 0.806 0.864 "66,063" 22 0.439 0.763 -0.154 427 " 13,521,443 " 16.4 5 82 0.200 BXD5 "75,548" "±1,273" "±2,846" 3.8% 6 "73,053" "78,043" "72,566" "79,857" "7,291" "±2,880" 2.31 63 18.9 514 48 0.969 1.872 "75,548" 49 0.990 0.080 2.319 514 " 8,101,344 " 15.9 5 80 0.200 BXD6 "62,695" ±801 "±1,961" 3.1% 7 "61,126" "64,264" "59,911" "64,712" "4,801" "±1,776" 0.23 163 19.8 392 25 0.500 0.000 "62,695" 5 0.092 0.159 -1.330 392 " 3,844,884 " 15.2 6 91 0.167 BXD8 "60,153" "±1,433" "±2,026" 3.4% 3 "57,344" "62,961" "57,856" "61,691" "3,834" "±1,419" -0.19 259 21.1 430 19 0.378 -0.312 "60,153" 26 0.520 #DIV/0! 0.051 430 " 4,106,221 " 15.2 2 30 0.500 BXD9 "66,053" "±1,611" "±3,602" 5.5% 6 "62,896" "69,210" "61,325" "69,342" "8,017" "±3,167" 0.77 185 22.8 439 39 0.786 0.792 "66,053" 30 0.602 -0.984 0.259 439 " 12,973,224 " 16.4 5 82 0.200 BXD11 "60,819" "±1,451" "±4,104" 6.7% 9 "57,975" "63,663" "56,124" "66,474" "10,351" "±3,488" -0.08 66 19.7 430 20 0.398 -0.259 "60,819" 24 0.480 0.418 -0.051 430 " 16,839,909 " 16.6 8 133 0.125 BXD12 "54,788" ±943 "±1,886" 3.4% 5 "52,940" "56,636" "53,045" "57,857" "4,813" "±2,069" -1.06 37 18.1 408 13 0.255 -0.659 "54,788" 14 0.276 -0.596 408 " 3,555,164 " 15.1 4 60 0.250 BXD13 "54,101" "±2,086" "±4,171" 7.7% 5 "50,014" "58,189" "56,328" "58,053" "1,725" "±1,019" -1.17 27 18.6 402 7 0.133 -1.114 "54,101" 10 0.194 0.204 -0.864 402 " 17,397,856 " 16.7 4 67 0.250 BXD14 "65,283" "±1,628" "±3,987" 6.1% 7 "62,093" "68,473" "59,564" "70,267" "10,704" "±3,960" 0.64 68 21.8 436 37 0.745 0.659 "65,283" 29 0.582 0.824 0.206 436 " 15,896,278 " 16.6 6 99 0.167 BXD15 "64,235" ±989 "±2,211" 3.4% 6 "62,298" "66,173" "61,004" "67,213" "6,208" "±2,669" 0.47 100 23.4 442 32 0.643 0.366 "64,235" 33 0.663 -0.265 0.421 442 " 4,887,563 " 15.4 5 77 0.200 BXD16 "64,255" "±2,517" "±6,166" 9.6% 7 "58,303" "70,208" "55,934" "75,614" "19,680" "±7,773" 0.48 64 21 465 35 0.704 0.536 "64,255" 43 0.867 0.211 1.114 465 " 38,021,869 " 17.5 6 105 0.167 BXD18 "54,266" "±1,147" "±2,565" 4.7% 6 "52,018" "56,514" "50,627" "58,177" "7,551" "±2,983" -1.14 113 20.3 413 9 0.173 -0.941 "54,266" 17 0.337 0.053 -0.421 413 " 6,577,362 " 15.7 5 78 0.200 BXD19 "67,558" "±1,581" "±4,337" 6.4% 9 "64,458" "70,657" "61,653" "74,741" "13,089" "±4,411" 1.01 108 19.5 423 44 0.888 1.215 "67,558" 21 0.418 0.023 -0.206 423 " 18,809,642 " 16.7 8 134 0.125 BXD20 "62,184" "±1,382" "±3,909" 6.3% 9 "59,475" "64,892" "55,879" "68,289" "12,410" "±4,182" 0.14 71 17.7 380 23 0.459 -0.102 "62,184" 4 0.071 0.325 -1.465 380 " 15,277,059 " 16.5 8 132 0.125 BXD21 "59,968" "±1,422" "±3,763" 6.3% 8 "57,180" "62,756" "54,618" "64,885" "10,267" "±3,799" -0.22 114 24.8 449 18 0.357 -0.366 "59,968" 36 0.724 0.025 0.596 449 " 14,160,186 " 16.5 7 115 0.143 BXD22 "64,802" "±1,869" "±3,738" 5.8% 5 "61,138" "68,466" "60,994" "70,785" "9,790" "±3,622" 0.57 38 17 430 36 0.724 0.596 "64,802" 25 0.500 -0.086 0.000 430 " 13,975,969 " 16.5 4 66 0.250 BXD23 "54,535" "±1,981" "±4,429" 8.1% 6 "50,653" "58,417" "50,750" "60,873" "10,123" "±3,999" -1.10 86 19.6 421 11 0.214 -0.792 "54,535" 20 0.398 0.009 -0.259 421 " 19,614,654 " 16.8 5 84 0.200 BXD24 "62,395" ±992 "±2,429" 3.9% 7 "60,451" "64,338" "58,714" "65,631" "6,917" "±2,559" 0.18 267 23.5 413 24 0.480 -0.051 "62,395" 16 0.316 0.311 -0.478 413 " 5,898,667 " 15.6 6 94 0.167 BXD25 "54,759" "±1,418" "±3,171" 5.8% 6 "51,979" "57,539" "50,469" "58,753" "8,283" "±3,562" -1.06 60 17.8 409 12 0.235 -0.723 "54,759" 15 0.296 0.262 -0.536 409 " 10,058,151 " 16.1 5 81 0.200 BXD27 "51,568" "±1,391" "±3,110" 6.0% 6 "48,842" "54,294" "48,194" "55,662" "7,468" "±3,211" -1.58 38 15.3 369 2 0.031 -1.872 "51,568" 2 0.031 0.995 -1.872 369 " 9,672,541 " 16.1 5 80 0.200 BXD28 "53,864" "±3,624" "±7,249" 13.5% 5 "46,761" "60,968" "44,367" "60,534" "16,167" "±7,857" -1.21 56 18.9 405 6 0.112 -1.215 "53,864" 12 0.235 -0.031 -0.723 405 " 52,542,002 " 17.8 4 71 0.250 BXD29 "63,539" "±1,859" "±4,156" 6.5% 6 "59,896" "67,182" "58,132" "69,862" "11,729" "±4,633" 0.36 65 19.7 398 29 0.582 0.206 "63,539" 7 0.133 0.508 -1.114 398 " 17,273,269 " 16.7 5 83 0.200 BXD30 "68,079" "±2,154" "±4,816" 7.1% 6 "63,858" "72,300" "58,132" "66,877" "8,745" "±4,250" 1.10 55 18.4 377 45 0.908 1.330 "68,079" 3 0.051 0.133 -1.635 377 " 23,192,379 " 17.0 5 85 0.200 BXD31 "66,833" "±2,038" "±4,556" 6.8% 6 "62,840" "70,827" "59,184" "71,842" "12,659" "±5,000" 0.90 51 21.3 415 42 0.847 1.023 "66,833" 18 0.357 -0.130 -0.366 415 " 20,757,645 " 16.8 5 84 0.200 BXD32 "76,836" "±3,355" "±7,502" 9.8% 6 "70,260" "83,412" "67,181" "85,632" "18,451" "±7,934" 2.52 47 19.6 429 49 0.990 2.319 "76,836" 23 0.459 0.817 -0.102 429 " 56,283,124 " 17.8 5 89 0.200 BXH4 "68,666" "±2,282" "±3,227" 4.7% 3 "64,194" "73,139" "49,213" "72,115" "22,902" "±9,848" 1.19 60 21.2 479 46 0.929 1.465 "68,666" 47 0.949 0.753 1.635 479 " 10,415,034 " 16.2 2 32 0.500 BXH6 "51,926" "±1,627" "±2,818" 5.4% 4 "48,737" "55,114" "65,719" "55,689" "-10,030" "-±4,313" -1.52 68 18 450 3 0.051 -1.635 "51,926" 38 0.765 0.861 0.723 450 " 7,940,112 " 15.9 3 48 0.333 BXH9 "56,967" "±2,238" "±3,876" 0.0% 4 "52,581" "61,353" "53,323" "62,366" "9,043" "±3,888" -0.70 72 24 477 16 0.316 -0.478 "56,967" 45 0.908 0.080 1.330 477 " 15,022,705 " 16.5 3 50 0.333 BXH12 "69,674" ±791 "±1,369" 2.0% 4 "67,479" "71,869" "68,179" "71,498" "3,319" "±1,613" 1.36 58 19 432 47 0.949 1.635 "69,674" 28 0.561 0.753 0.154 432 " 1,875,437 " 14.4 3 43 0.333 BXH14 "66,023" "±3,297" "±7,372" 11.2% 6 "57,956" "74,091" "54,231" "72,980" "18,749" "±8,062" 0.76 51 19 421 38 0.765 0.723 "66,023" 19 0.378 0.861 -0.312 421 " 54,350,801 " 17.8 5 89 0.200 BXH19 "54,136" "±2,660" "±4,608" 8.5% 4 "46,749" "61,522" "48,761" "59,761" "11,000" "±5,346" -1.16 44 18 432 8 0.153 -1.023 "54,136" 27 0.541 0.080 0.102 432 " 21,232,982 " 16.9 3 51 0.333 C3H/HeJ "67,039" "±1,625" "±3,634" 5.4% 6 "63,854" "70,224" "60,818" "71,259" "10,441" "±4,124" 0.93 85 29.8 510 14.1 H inbred c/c-rd/rd done 43 0.867 1.114 "67,039" 48 0.969 1.872 510 " 13,204,312 " 16.4 5 82 0.200 C57BL/6JAx1 "66,082" "±1,605" "±4,540" 6.9% 9 "62,451" "69,713" "58,641" "71,770" "13,129" "±4,424" 1.16 78 19.5 459 41 0.827 0.941 "66,082" 40 0.806 0.581 0.864 459 " 20,611,798 " 16.8 8 135 0.125 C57BL/6J "54,320" "±1,347" "±5,216" 9.6% 16 "51,680" "56,960" "47,070" "65,653" "18,582" "±6,039" -1.13 79 19.5 465 14.7 "16,895" ±256 3.2 L inbred c/c and +/+ 3 in prog 10 0.194 -0.864 "54,320" 42 0.847 0.298 1.023 465 " 27,205,954 " 17.1 15 257 0.067 C57BLKS/J "64,238" "±2,572" "±6,301" 9.8% 7 "59,196" "69,279" "9,319" "82,621" "73,302" "±23,823" 0.48 51 18.3 442 - "14,595" ±900 4.4 H inbred "2 males in prog, 1 replacement feamle ordered Feb 95" 33 0.663 0.421 "64,238" 32 0.643 0.844 0.366 442 " 39,698,606 " 17.5 6 105 0.167 CBA/CaJ "56,028" "±1,145" "±2,561" 4.6% 6 "53,784" "58,273" "52,809" "60,281" "7,472" "±2,951" -0.86 45 16.6 440 - ? inbred 2 in prog 15 0.296 -0.536 "56,028" 31 0.622 0.312 440 " 6,556,583 " 15.7 5 78 0.200 CE/J "63,593" "±2,384" "±4,767" 7.5% 5 "58,921" "68,265" "55,233" "66,888" "11,655" "±5,012" 0.37 96 23.9 462 - H inbred 1 in prog 30 0.602 0.259 "63,593" 41 0.827 -0.030 0.941 462 " 22,728,117 " 16.9 4 68 0.250 DBA/2J "63,351" "±1,137" "±4,100" 6.5% 13 "61,122" "65,579" "57,625" "69,775" "12,150" "±3,730" 0.33 55 16.9 401 - "15,435" ±320 4.1 H inbred 6 in progress 27 0.541 0.102 "63,351" 9 0.173 -0.047 -0.941 401 " 16,807,933 " 16.6 12 200 0.083 LP/J "52,225" "±1,919" "±5,078" 9.7% 8 "48,463" "55,987" "46,158" "59,057" "12,899" "±4,528" -1.47 108 21.7 365 - L inbred A/A done 4 0.071 -1.465 "52,225" 1 0.010 0.532 -2.319 365 " 25,787,354 " 17.1 7 119 0.143 NZB/BinJ "61,135" "±1,226" "±2,742" 4.5% 6 "58,731" "63,538" "56,090" "63,967" "7,878" "±3,112" -0.03 118 27.5 471 - ? inbred 3 in prog 21 0.418 -0.206 "61,135" 44 0.888 1.215 471 " 7,518,005 " 15.8 5 79 0.200 NZW/LacJ "64,253" "±1,444" "±2,501" 3.9% 4 "61,423" "67,083" "62,117" "67,765" "5,648" "±2,745" 0.48 159 31.8 459 - H inbred c/c 1 in prog 34 0.684 0.478 "64,253" 39 0.786 0.792 459 " 6,252,909 " 15.6 3 47 0.333 PL/J "55,976" "±1,263" "±3,341" 6.0% 8 "53,501" "58,451" "50,498" "60,287" "9,789" "±3,436" -0.86 43 18.3 446 - L inbred c/c-rd/rd done 14 0.276 -0.596 "55,976" 34 0.684 0.478 446 " 11,162,525 " 16.2 7 114 0.143 SJL/J "52,473" "±1,684" "±3,766" 7.2% 6 "49,172" "55,775" "51,788" "56,439" "4,651" "±1,837" -1.43 42 15.8 400 L inbred rd/rd 3 in prog 5 0.092 -1.330 "52,473" 8 0.153 -0.842 -1.023 400 " 14,186,265 " 16.5 5 82 0.200 Average "61,307" "±1,711" "±3,905" ±0.36% 6.59 6.4% "11,153" "±4,266" 82 20.4 431 correlations: 0.975 correlations: 0.993 Bartlett's M= 100.90 804.1 274 Strain SD and SEM "±6,168" ±645 "±1,416" 2.5% Correl with RGC 0.056 0.256 0.386 0.14863 0.267 C= 1.087 Count 49 49 49 6.27% 323 Correl with brain 0.047 0.505 — 2.520 M/C 92.844 with df of a-1 48 0.0196 0.7218951 ±2.819= corrected average SD 0.1196 is correlation between CV and RGC number r crit 5% with 20 df is 0.423 49 See Snedecor and Cochran (1980) p 252. ±7.02 4.5% 0.211111111 2.6% 0.720 0.830 0.078 Standard Inbred Only "t dist, 2-tailed, p=0.025 per tail)" 0.949 Strain Neurons SEM SD CV N 95%CI- 95%CI+ Lowest Highest Range SD_Range Z High_Low Age Body Brain BrainSE BrainSD BrainCV BrainN Type Note Rank_RGC Z_Frac_RGC Z_exp_RGC Rank_Brain Z_Frac_Brain Z_exp_Brain Brain Z_exp RGC Females %Females 129/J "63,772" "±1,699" "±4,163" 6.5% 7 "59,754" "67,791" "57,185" "68,409" "11,223" "±4,153" 0.72 H 76 20.0 407 ±5.2 ±15 3.8% 10 c/c done 13 0.735 0.629 6 0.324 -0.458 407 0.629 "63,772" 3 43% A/J "51,238" "±1,362" "±3,602" 7.0% 8 "48,098" "54,377" "46,312" "56,720" "10,408" "±3,653" -1.65 L 111 22.2 395 ±12.3 ±30 7.7% 7 A/A done. Ordered 1 male and 1 female for F1 production. High incidence of cleft palate. 1 0.029 -1.890 3 0.147 -1.049 395 -1.890 "51,238" 3 38% AKR/J "62,788" ±890 "±1,990" 3.2% 6 "60,611" "64,966" "60,063" "65,143" "5,081" "±2,007" 0.53 H 71 NA 461 ±12.4 ±28 6.0% 6 c/c done. A short-lived strain 9 0.500 0.000 12 0.676 0.458 461 0.000 "62,788" 5 83% BALB/cBy "57,558" "±1,768" "±4,330" 7.5% 7 "53,379" "61,738" "51,320" "63,924" "12,604" "±4,663" -0.46 ? 30 14.4 403 ±11.9 ±31 7.8% 8 c/c done. Males are not aggressive. Separated by 100 generations from BALB/cJ. Ok breeders. 7 0.382 -0.299 5 0.265 -0.629 403 -0.299 "57,558" 5 71% BALB/cJ "63,393" "±2,210" "±5,846" 9.2% 8 "58,298" "68,488" "53,252" "68,788" "15,536" "±5,453" 0.65 H 96 23.9 477 ±9.0 ±22 4.6% 7 c/c 3 in prog. Males are highly aggressive. Poor breeders. 11 0.618 0.299 16 0.912 1.352 477 0.299 "63,393" 4 50% C3H/HeJ "67,039" "±1,625" "±3,634" 5.4% 6 "63,063" "71,015" "60,818" "71,259" "10,441" "±4,124" 1.34 H 85 29.8 510 ±4.2 ±6 1.2% 3 c/c-rd/rd done 17 0.971 1.890 17 0.971 1.890 510 1.890 "67,039" 5 83% C57BL/6JAx1 "66,082" "±1,605" "±4,540" 6.9% 9 "62,451" "69,713" "58,641" "71,770" "13,129" "±4,858" 1.16 H 78 19.5 459 ±5.3 ±17 3.6% 11 +/+ These cases all from Annex 1. Ordered 6 more not from Annex 1. 16 0.912 1.352 11 0.618 0.299 459 1.352 "66,082" 4 44% C57BL/6J "54,320" "±1,347" "±5,216" 9.6% 16 "51,465" "57,175" "47,070" "65,653" "18,582" "±6,039" -1.07 L 79 21.5 472 ±4.5 ±25 5.2% 31 "+/+, c/c" Original set prior to 1994. Includes pigemented and coisogenic albinos 4 0.206 -0.821 15 0.853 1.049 472 -0.821 "54,320" 4 25% C57BLKS/J "64,238" "±2,572" "±6,301" 9.8% 7 "58,155" "70,320" "9,319" "82,621" "73,302" "±23,823" 0.81 ? 114 21.0 445 ±4.6 ±14 3.1% 10 3 in prog 14 0.794 0.821 8 0.441 -0.148 445 0.821 "64,238" 10 143% CBA/CaJ "56,028" "±1,145" "±2,561" 4.6% 6 "53,226" "58,831" "52,809" "60,281" "7,472" "±2,951" -0.75 L 45 16.6 440 ±16.3 ±16 3.7% 2 no rd done 6 0.324 -0.458 7 0.382 -0.299 440 -0.458 "56,028" 3 50% CE/J "63,593" "±2,384" "±4,767" 7.5% 5 "57,465" "69,720" "55,233" "66,888" "11,655" "±5,012" 0.69 H 96 23.9 462 ±7.1 ±16 3.4% 6 c^e/c^e. Dirty white coat. done 12 0.676 0.458 13 0.735 0.629 462 0.458 "63,593" 1 20% DBA/2J "63,351" "±1,137" "±4,100" 6.5% 13 "60,894" "65,807" "57,625" "69,775" "12,150" "±3,730" 0.64 H 55 16.9 394 ±7.6 ±23 5.8% 10 3 in prog 10 0.559 0.148 2 0.088 -1.352 394 0.148 "63,351" 6 46% LP/J "52,225" "±1,919" "±5,078" 9.7% 8 "47,799" "56,651" "46,158" "59,057" "12,899" "±4,528" -1.47 L 108 21.7 365 ±8.3 ±18 5.1% 6 A/A done 2 0.088 -1.352 1 0.029 -1.890 365 -1.352 "52,225" 4 50% NZB/BinJ "61,135" "±1,226" "±2,742" 4.5% 6 "58,134" "64,135" "56,090" "63,967" "7,878" "±3,112" 0.22 H 118 27.5 471 ±7.9 ±11 2.4% 3 done 8 0.441 -0.148 14 0.794 0.821 471 -0.148 "61,135" 3 50% NZW/LacJ "64,253" "±1,444" "±2,501" 3.9% 4 "60,245" "68,261" "62,117" "67,765" "5,648" "±2,745" 0.81 H 159 31.8 459 — — — — c/c done 15 0.853 1.049 10 0.559 0.148 459 1.049 "64,253" 2 50% PL/J "55,976" "±1,263" "±3,341" 6.0% 8 "53,064" "58,888" "50,498" "60,287" "9,789" "±3,436" -0.76 L 43 18.3 446 ±14.1 ±20 4.5% 3 c/c-rd/rd done 5 0.265 -0.629 9 0.500 0.000 446 -0.629 "55,976" 2 25% SJL/J "52,473" "±1,684" "±3,766" 7.2% 6 "48,352" "56,595" "45,570" "56,439" "10,869" "±4,293" -1.42 L 42 15.8 400 ±9.4 ±19 4.7% 5 rd/rd done 3 0.147 -1.049 4 0.206 -0.821 400 -1.049 "52,473" 4 67% Average "59,968" "±1,605" "±4,028" 6.76% 7.65 "14,627" "±5,211" 83 21.5 439 ±19 4.5% 8.00 0.94851838 Correlation: 0.972 0.949 68 55% Strain SD and SEM "±5,277" ±461 "±1,201" 0.51% " 1,226.12 " Correl with RGC 0.260 0.440 0.583 0.340 128 Count 17 ±300 2.1% 130 Correl with brain 0.230 0.598 —- F (Sept 95) 11.4 with 16 and 113 df P=6E-17 3.6% r crit 5% with 15 df is 0.482 F1 isogenic hybrids "t dist, 2-tailed, p=0.025 per tail)" Strain Neurons SEM SD CV N 95%CI- 95%CI+ Lowest Highest Range SD_Range Mat_Strain Pat_Strain MidParent Diff [d] [h] Potency_ratio High_Low Age Body Brain BrainSD BrainCV Action Type (FxM) RGC-Brain Correl B6AxCF1/JGo "64,854" "±2,408" "±5,898" 9.1% 7 "59,160" "70,547" "56,718" "74,205" "17,487" "±6,470" "66,082" "63,393" "64,738" 116 "1,345" 116 0.09 I 65 21.6 478 ±26 additive C57BL/6JAx1 by BALB/cJ 0.586 CB6AxF1/J (?) "66,688" "±1,094" "±2,446" 3.7% 6 "64,011" "69,365" "63,427" "70,088" "6,661" "±2,631" "63,393" "66,082" "64,738" "1,950" "1,345" "1,950" 1.45 H 50 22.8 517 — over-dominance BALB/c by C57BL/6J n=2 CAF1/J "56,147" "±1,794" "±4,010" 7.1% 6 "51,758" "60,535" "51,815" "62,614" "10,799" "±4,266" "63,393" "51,238" "57,315" "-1,169" "6,078" "1,169" 0.19 I 36 20.0 468 ±10 additive BALB/c by A/J 0.694 CCASF1 "59,705" "±1,278" "±3,615" 6.1% 9 "56,814" "62,597" "55,238" "64,436" "9,198" "±3,100" "63,393" "45,484" "54,439" "5,267" "8,954" "5,267" 0.59 I 121 25.8 492 ±27 weak dominance BALB/c by CAST/Ei 0.303 PLSJF1/J "56,096" "±1,279" "±3,134" 5.6% 7 "53,071" "59,120" "50,030" "58,973" "8,944" "±3,309" "55,976" "52,473" "54,225" "1,871" "1,751" "1,871" 1.07 I 54 21.0 438 ±12 additive PL/J by SJL/J -0.001 Average "60,698" "±1,571" "±3,821" 6.31% 7.00 "10,618" "±3,955" "1,607" "3,894" 65 22.2 478 19 Strains SD "±4,900" ±536 1.00% Correl with RGC 0.057 0.296 0.783 predicted average "±59,091" excess= "1,607" 2.0% 3.09% Correl with brain 0.550 Count 5 corrected 2.71% 35 r crit 5% with 3 df is 0.878 See BXD Residuals Spreadsheet "B <62,000)" "t dist, 2-tailed, p=0.025 per tail)" <425 = D type "Proximal Chr 11: D11Mit2, D11Byu1" "Belknap JK, Phillips TJ, O'Toole LA (1992) Quantitative trait loci associaed with brain weight in the BXD/Ty recombinant inbred mouse strains. Brain Res Bull 29:337-344. 8 males per strain" BXD RI Strains Neurons SEM SD CV N RGC_Class1* RGC_Class2** Tstap91A Z 95%CI- 95%CI+ Lowest Highest Range SD_Range Age Body Brain BrainSE BrainSD BrainCV BrainN Brain_Class D11Ncvs74 Belknap_BXD ±SD Note Rank_RGC Z_Fract Z_Exp RGCs Rank_Brain Z_Fract_Brain Z_Exp_Brain Brain Cerebellum "Redone Jan 9, 96" C57BL/6J "54,320" "±1,347" "±5,216" 9.6% 16 B B B -1.00 "51,465" "57,175" "47,070" "65,653" "18,582" "±5,853" 79 21.5 472 ±5.3 ±29 6.1% 31 B B 465 ±23 5 0.161 -0.992 "54,320" 27 0.855 1.059 472 From BW For QTL mapping Reference for r 2 grade Strain Actual Predicted Residuals Tstap91A Residuals BXD1 "61,996" "±1,077" "±3,231" 5.2% 10 U U B 0.70 "59,596" "64,396" "59,552" "67,167" "7,615" "±2,673" 51 20.5 448 ±6.9 ±23 5.1% 12 B B 450 ±22 "done, 4 more received from JAX via Dan, July 95" 12 0.411 -0.226 "61,996" 24 0.758 0.701 448 BXD1 61425 " 64,851 " "-3,426" 0 0 0 BXD2 "66,063" "±1,644" "±3,677" 5.6% 6 D D D 1.61 "62,039" "70,087" "60,417" "70,774" "10,358" "±4,091" 53 23.1 427 ±8.2 ±18 4.3% 6 B B 390 ±19 done 23 0.804 0.854 "66,063" 16 0.500 0.000 427 BXD2 66063 " 62,575 " "3,488" 1 1 1 BXD5 "75,548" "±1,273" "±2,846" 3.8% 6 D D D 3.72 "72,433" "78,662" "72,566" "79,857" "7,291" "±2,880" 63 18.9 514 ±8.7 ±23 4.5% 8 B B 477 ±27 "done, 3 breeders in colony Aug 95" 27 0.946 1.611 "75,548" 28 0.887 1.213 514 51 BXD5 75548 " 71,826 " "3,721" 1 1 1 BXD6 "62,695" ±801 "±1,961" 3.1% 7 D D D 0.86 "60,802" "64,588" "59,911" "64,712" "4,801" "±1,776" 163 19.8 392 ±3.6 ±9 2.4% 8 D D 361 ±16 done 15 0.518 0.045 "62,695" 4 0.113 -1.211 392 43 BXD6 62695 " 58,949 " "3,746" 1 1 1 BXD8 "60,153" "±1,433" "±2,026" 3.4% 3 B U D 0.29 "55,592" "64,713" "57,856" "61,691" "3,834" "±1,419" 259 21.1 430 ±3.6 ±11 2.5% 10 B B 421 ±13 done. Could use 3 young mice. I suspect the low average. 10 0.339 -0.414 "60,153" 20 0.629 0.330 430 BXD8 56905 " 62,943 " "-6,038" 1 0 0 BXD9 "66,053" "±1,611" "±3,602" 5.5% 6 D D D 1.60 "62,112" "69,995" "61,325" "69,342" "8,017" "±3,167" 185 22.8 439 ±11.3 ±16 3.6% 3 B B 419 ±15 done 22 0.768 0.732 "66,053" 22 0.694 0.507 439 BXD9 65732 " 63,917 " "1,815" 1 1 1 BXD11 "60,819" "±1,451" "±4,104" 6.7% 9 B U B 0.44 "57,537" "64,101" "56,124" "66,474" "10,351" "±4,089" 66 19.7 430 ±4.0 ±12 2.8% 10 B B NA NA "4 more received from Jax by Dan, July 95" 11 0.375 -0.319 "60,819" 18 0.565 0.163 430 BXD11 60757 " 62,894 " "-2,138" 0 0 0 BXD12 "54,788" ±943 "±1,886" 3.4% 5 B B B -0.90 "52,365" "57,211" "53,045" "57,857" "4,813" "±2,069" 37 18.1 408 ±6.4 ±6 1.6% 2 D B 412 ±13 done 8 0.268 -0.619 "54,788" 9 0.274 -0.600 408 BXD12 53553 " 60,633 " "-7,080" 0 0 0 BXD13 "54,101" "±2,086" "±4,171" 7.7% 5 B B B -1.05 "48,740" "59,462" "56,328" "58,053" "1,725" "±1,019" 27 18.6 402 ±4.5 ±9 2.2% 5 D D 403 ±9 "First three all males. 1 more received by Dan at Jax, July 95" 3 0.089 -1.345 "54,101" 7 0.210 -0.807 402 BXD13 54665 " 59,988 " "-5,322" 0 0 0 BXD14 "65,283" "±1,628" "±3,987" 6.1% 7 D D D 1.43 "61,434" "69,132" "59,564" "70,267" "10,704" "±3,960" 68 21.8 436 ±6.0 ±15 3.4% 7 B D 439 ±9 done 21 0.732 0.619 "65,283" 21 0.662 0.417 436 BXD14 65996 " 63,523 " "2,473" 1 1 1 BXD15 "64,235" ±989 "±2,211" 3.4% 6 D U D 1.20 "61,816" "66,655" "61,004" "67,213" "6,208" "±2,452" 100 23.4 442 ±9.4 ±21 4.8% 6 B B 429 ±14 Need 4 more because of low residuals after brain weight regression 18 0.625 0.319 "64,235" 23 0.726 0.601 442 BXD15 63765 " 64,224 " -459 1 weak 0 BXD16 "64,255" "±2,517" "±6,166" 9.6% 7 D U D 1.20 "58,303" "70,208" "55,934" "75,614" "19,680" "±7,773" 64 21.4 465 ±7.4 ±20 4.2% 8 B B 436 ±16 "3 received May 95, additional 4 from Jax by Dan, July 95" 19 0.661 0.414 "64,255" 26 0.823 0.926 465 48 BXD16 65109 " 66,425 " "-1,315" 1 weak 0 BXD18 "54,266" "±1,147" "±2,565" 4.7% 6 B B B -1.02 "51,459" "57,072" "50,627" "58,177" "7,551" "±2,983" 113 20.3 413 ±8.0 ±14 3.3% 4 D D 427 ±13 1 in prog 4 0.125 -1.150 "54,266" 12 0.371 -0.329 413 BXD18 55743 " 61,183 " "-5,440" 0 0 0 BXD19 "67,558" "±1,581" "±4,337" 6.4% 9 D D D 1.94 "63,981" "71,135" "61,653" "74,741" "13,089" "±4,411" 108 19.5 423 ±6.7 ±19 4.5% 9 B B NA NA done 25 0.875 1.150 "67,558" 15 0.468 -0.081 423 BXD19 67624 " 62,238 " "5,386" 1 1 1 BXD20 "62,184" "±1,382" "±3,909" 6.3% 9 U U D 0.74 "59,058" "65,310" "55,879" "68,289" "12,410" "±4,356" 71 17.7 380 ±3.9 ±13 3.4% 12 D D NA NA 1 in prog 13 0.446 -0.135 "62,184" 3 0.081 -1.401 380 41 BXD20 61915 " 57,624 " "4,291" 1 1 1 BXD21 "59,968" "±1,422" "±3,763" 6.3% 8 B U D 0.25 "56,688" "63,248" "54,618" "64,885" "10,267" "±3,799" 114 24.8 449 ±7.3 ±19 4.3% 8 B B 409 ±20 done 9 0.304 -0.514 "59,968" 25 0.791 0.809 449 45 BXD21 59236 " 64,964 " "-5,728" 1 0 0 BXD22 "64,802" "±1,869" "±3,738" 5.8% 5 D D D 1.33 "59,997" "69,607" "60,994" "70,785" "9,790" "±4,210" 38 17.3 430 ±5.4 ±11 2.5% 5 B B 431 ±10 3 in progress 20 0.696 0.514 "64,802" 19 0.597 0.246 430 BXD22 64802 " 62,908 " "1,894" 1 1 1 BXD23 "54,535" "±1,981" "±4,429" 8.1% 6 B B B -0.96 "49,689" "59,381" "50,750" "60,873" "10,123" "±3,999" 86 19.6 421 ±8.0 ±18 4.3% 6 D B 432 ±30 1 in prog. 2 backorders canceled May 95 6 0.196 -0.854 "54,535" 14 0.436 -0.162 421 BXD23 54535 " 62,005 " "-7,470" 0 0 0 BXD24 "62,395" ±992 "±2,429" 3.9% 7 D U D 0.79 "60,050" "64,739" "58,714" "65,631" "6,917" "±2,559" 267 23.5 413 ±6.9 ±18 4.4% 8 D D 380 ±17 done 14 0.482 -0.045 "62,395" 11 0.339 -0.416 413 BXD24 62254 " 61,108 " "1,146" 1 weak 1 BXD25 "54,759" "±1,418" "±3,171" 5.8% 6 B B B -0.91 "51,288" "58,229" "53,110" "58,753" "5,643" "±2,229" 60 17.8 409 ±5.5 ±13 3.3% 7 D B 394 ±16 1 back-ordered? Cut other side of case H 7 0.232 -0.732 "54,759" 10 0.307 -0.506 409 BXD25 55768 " 60,742 " "-4,974" 0 0 0 BXD27 "51,568" "±1,391" "±3,110" 6.0% 6 B B B -1.62 "48,165" "54,971" "48,194" "55,662" "7,468" "±2,950" 41 15.5 365 ±6.9 ±15 4.2% 6 D D 381 ±18 done: One case had hydrocephaly. 1 0.018 -2.100 "51,568" 1 0.016 -2.141 365 42 BXD27 51568 " 56,055 " "-4,487" 0 0 0 BXD28 "53,864" "±3,624" "±7,249" 13.5% 5 B B B -1.11 "44,548" "63,181" "44,367" "60,534" "16,167" "±7,857" 56 18.9 405 ±7.7 ±17 4.3% 6 D D 389 ±20 2 in colony 2 0.054 -1.611 "53,864" 8 0.242 -0.700 405 BXD28 51114 " 60,323 " "-9,209" 0 0 0 BXD29 "63,539" "±1,859" "±4,156" 6.5% 6 D U D 1.05 "58,991" "68,087" "58,132" "69,862" "11,729" "±4,633" 65 19.7 398 ±8.0 ±18 4.5% 6 D D 375 ±11 done 17 0.589 0.226 "63,539" 6 0.177 -0.925 398 BXD29 63539 " 59,513 " "4,026" 1 1 1 BXD30 "68,079" "±2,154" "±4,816" 7.1% 6 D D D 2.06 "62,809" "73,349" "61,398" "72,960" "11,562" "±4,971" 55 18.4 377 ±5.6 ±14 3.6% 7 D D NA NA "4 sent by Dan from JAX, July 95" 26 0.911 1.345 "68,079" 2 0.048 -1.661 377 BXD30 68079 " 57,336 " "10,743" 1 1 1 BXD31 "66,833" "±2,038" "±4,556" 6.8% 6 D D B 1.78 "61,848" "71,819" "59,184" "71,842" "12,659" "±5,000" 51 21.3 415 ±6.9 ±15 3.7% 6 D D NA NA done 24 0.839 0.992 "66,833" 13 0.403 -0.245 415 BXD31 65983 " 61,317 " "4,665" 0 1 1 BXD32 "76,836" "±3,355" "±7,502" 9.8% 6 D D D 4.00 "68,626" "85,046" "67,181" "85,632" "18,451" "±7,934" 47 19.6 429 ±2.6 ±11 2.5% 18 B D NA NA 4 in progress 28 0.982 2.100 "76,836" 17 0.532 0.081 429 BXD32 76836 " 62,872 " "13,964" 1 1 1 Correlations 0.670 0.264 0.618 0.740 0.632 DBA/2J "63,351" "±1,137" "±4,100" 6.5% 13 D D D 1.00 "60,894" "65,807" "57,625" "69,775" "12,150" "±3,730" 52 17.0 394 ±7.6 ±23 5.8% 10 D D 339 ±19 16 0.554 0.135 "63,351" 5 0.145 -1.057 394 critical with N of 25 =0.659 italic have weak residuals Average "61,959" "58,835" "±3,831" 6.17% 6.5 *Liberal **Stringent "9,585" "±3,818" 89 20.1 422 ±16 3.6% "F=14.77, p-.00083 with D11Ncvs74" 413 ±29 Belknap set average (no olfactory bulb) bold have discrepant residuals Count 28 26 766 0.46% 168 No alternative correl with RGC 0.017 0.259 0.489 0.24 426 ±29 Our equivalent set average (with bulb) SD brain ±31.0 explain 45% SD across BXD "±4,498" 2.37% "Tstap91A (at 45 cM, 2 cM distal to Hoxb)" Jan 18 1996 QT Map Manager Links report correl with brain 0.060 0.444 — r= 0.82 Our BXD brains to Belknap's BXD brains Average D type "65,467" 15 5.1% F= "27.65 (with truncation of BXD5, and 32 to 68,000" Average B type "54,754" 8 5.5% p= 0.0000217 "2 in 100,000" r crit 5% with 20 df is 0.423 Average all BXDs "62,199" 23 5.3% 0.7185629 approx lod 4.60 if r=.443 then z = 0.475 F (Nov 95) of 18.7 with 25 and 156 df <450=B BXH RI Strains Neurons SEM SD CV N RGC_Class1* "Chr 2, Hdc" Chr 11 Mpmv4 (48 cM) Z 95%CI- 95%CI+ Lowest Highest Range SD_Range Age Body Brain BrainSE BrainSD BrainCV BrainN Brain_Class Cerebellum Belknap_Brain ±SD Note Rank_RGC Z_Fract Z_Exp RGCs Rank_Brain Z_Fract_Brain Z_Exp_Brain Brain C57BL/6J "54,320" "1,347" "5,216" 9.6% 16 B B -1.00 "51,465" "57,175" "47,070" "65,653" "18,582" "±5,853" 79 19.5 465 ±29 6.2% 465 ±23 6 0.196 -0.854 "54,320" #N/A #N/A #N/A 465 BXH2 "63,406" "4,397" "4,397" 6.9% 2 H B H "44,486" "82,327" 42 19 414 ±1 ±1 0.3% 2 B BXH3 "59,642" 1 U B H 47 20 461 ±15 ±15 3.3% 2 H BXH4 "68,666" "2,282" "3,227" 4.7% 3 H H H "61,404" "75,929" 60 21 479 ±14 ±25 5.2% 4 H BXH6 "51,926" "1,627" "2,818" 5.4% 4 B B H "47,409" "56,443" 68 18 450 ±8 ±15 3.2% 4 B BXH7 "60,779" 1 U B H 38 17 448 ±0 ±0 0.1% 2 B BXH8 "64,631" 1 H H H 27 12.0 395 ±1 ±1 0.2% 2 B BXH9 "56,967" "2,238" "3,876" 6.8% 4 B B B "50,754" "63,180" 72 24 477 ±3 ±5 1.1% 4 H BXH10 "64,931" 1 H H B 35 18 448 ±19 ±19 4.2% 2 B BXH11 "51,312" 1 B B B 54 20 422 1 B BXH12 "69,674" 791 "1,369" 2.0% 4 H H H "67,479" "71,869" "68,179" "71,498" "3,319" 58 19 432 ±3 ±4 1.0% 3 B BXH14 "66,023" "3,297" "7,372" 11.2% 6 H H H "57,956" "74,091" "54,231" "72,980" "18,749" 51 19 421 ±4 ±9 2.1% 6 B BXH19 "54,136" "2,660" "4,608" 8.5% 4 B B B "46,749" "61,522" "48,761" "59,761" "11,000" 44 18 432 ±13 ±22 5.0% 4 B C3H/HeJ "67,039" "±1,625" "±3,634" 5.4% 6 H H "63,063" "71,015" "59,061" "71,259" "12,197" "±3,842" 88 30 510 ±4 ±6 1.2% 3 H Midpoint "60,679" 32 Linkage stats of Jan 24 96: "best QTL match to Hdc on Chr 2 (p=0.001, F=20.9)" 440 ±8 ±30 2.5% No locus on Chr 11 with this pattern. Count 12 SD across BXH "6,290" Average H type "68,121" Average B type "54,138" Average all BXHs "61,008" "t dist, 2-tailed, p<0.025 per tail)" Outcrossed Strains Neurons SEM SD N CV H or L 95%CI- 95%CI+ Lowest Highest Range SD_Range Age Body Brain BrainSD BrainCV Brain_Neuron_Correl Brain_Neuron_Covariation Brain_Body_Corr CARL/RpGo "51,263" "±2,214" "±7,000" 11 13.7% I "46,391" "56,135" "39,546" "62,844" "23,298" "±7,339" 283 20.1 418 ±24 5.7% NA CCASF2/JGo "56,617" "±1,447" "±8,683" 75 15.3% I "53,734" "59,500" "40,595" "77,548" "36,954" "±9,904" 40 16.4 443 ±37 8.4% 0.038 2370 0.738 CD-1/Go "68,338" "±2,163" "±7,720" 14 11.3% H "63,699" "72,978" "57,243" "87,242" "29,998" "±8,820" 94 NA NA NA NA Average "58,739" "±7,801" 13.4% ±1.4% "±8,687" 7.1% SD "±8,733" 100 0.1225114 Count 3 0.12757351 "t dist, 2-tailed, p<0.025 per tail)" Wild Inbred Strains Neurons SEM SD N CV H or L 95%CI- 95%CI+ Lowest Highest Range SD_Range Z Type Age Body Brain BrainSD BrainCV Status RGC-Brain Correl CASA/Rk "48,917" "±2,958" "±5,123" 4 10.5% L "40,705" "57,128" "45,131" "56,171" "11,041" "±5,366" -0.65 Mus musculus castaneus 51 9.7 342 ±18 5.4% done 0.265 CAST/Ei "45,484" ±999 "±3,160" 11 6.9% L "43,285" "47,684" "41,341" "50,160" "8,819" "±2,778" -1.08 Mus musculus castaneus 153 13.4 373 ±31 8.4% done 0.427 CZECHII/Ei "59,207" "±3,341" "±8,183" 7 13.8% I "51,307" "67,106" "49,312" "73,647" "24,335" "±9,004" 0.66 Mus musculus musculus 116 12.6 350 ±16 4.5% done -0.125 MOLD/Rk "43,758" "±4,207" "±8,414" 5 19.2% L "32,943" "54,573" "32,348" "52,400" "20,052" "±9,745" -1.30 Mus musculus molossinus (rd/rd) 163 13.5 323 ±9 2.9% done (should get some young ones or other MOL strains) n=2 PANCEVO/Ei "64,300" "±1,316" "±2,943" 6 4.6% H "61,079" "67,520" "61,473" "67,279" "5,806" "±2,293" 1.30 Mus spicilegus (hortulanus) 145 15.3 436 ±12 2.8% done -0.730 SPRET/Ei "59,049" "±1,804" "±4,035" 6 6.8% I "54,634" "63,464" "53,323" "64,447" "11,124" "±4,394" 0.64 Mus spretus 33 10.2 336 ±25 7.6% done -0.034 WSB/Ei "57,380" "±1,698" "±4,492" 8 7.8% I "53,465" "61,295" "50,826" "63,021" "12,195" "±4,280" 0.43 Mus musclus domesticus 106 15.8 423 ±20 4.7% done 0.060 Average "54,014" "±5,193" 6.7 10.0% or 7.9±1.0 is CZECHII/Ei is excluded "±5,409" 369 ±19 5.2% Count 7 47 ±2.1% ±1.7% Strains SD "±7,888" 8.17% 7.11% when MOLD/Rk low case is excluded correl with RGC -0.255 0.231 0.564 0.32 F (Sept 95) =14.6 with 6 and 40 df and P=9E-9 correl with brain 0.294 0.769 — "t dist, 2-tailed, p<0.025 per tail)" Strain or Species RGCs SEM SD N CV H or L 95%CI- 95%CI+ Lowest Highest Range Type Note Age Body Brain BrainSD BrainCV N brain Correl RGC to brain 4.91161432 CAST/Ei = CAS "45,484" ±999 "±3,160" 11 6.9% L "43,285" "47,684" "41,341" "50,160" "8,819" Mus castaneus wild inbred strain 124 11.4 373 ±31 8.4% 4.0000 CCASF1/JGo "59,705" "±1,278" "±3,615" 9 6.1% I "56,814" "62,597" "55,238" "64,436" "9,198" BALB/c female by CAST/Ei male 161 24.3 489 ±27 5.5% CCASF2 "56,617" "±1,447" "±8,683" 75 15.3% I "53,734" "59,500" "33,515" "77,548" "44,033" BCF1 intercrossF2 progeny 40 15.6 428 ±34 7.9% 115 0.329 0.10838253 CCASCASN2/JGo "56,673" "±2,970" "±6,642" 6 11.7% I "49,406" "63,941" "50,317" "64,895" "14,578" BCF1 by C backcross progeny 39 15.2 417 ±30 7.2% CCASCN2 "60,679" "±1,571" "±3,847" 7 6.3% I "56,965" "64,393" "53,093" "63,409" "10,317" BCF1 by B backcross progeny As many as 40 cases embedded. Half brains sent to Wahlsten for corpus callosum analysis. 32 16.2 467 ±26 5.6% BALC/c = C "63,393" "±2,210" "±5,846" 8 9.2% H "58,298" "68,488" "53,252" "68,788" "15,536" BALB/c parent strain 96 23.9 477 ±22 4.6% count 6 116 midpoint "54,439" F2 male SE female SE r age body brain age 42 ±13 39 ±5 age body 16.5 ±5 15.1 ±3 body 0.743 brain 436 ±39 423 ±31 brain 0.487 0.738 RGC "58,044" "±9,927" "55,554" "±8,407" RGC 0.049 0.174 0.329 0.108383 RGC N 31 41 Brain N 45 64 Strain or Species RGCs SEM SD N CV H or L 95%CI- 95%CI+ Lowest Highest Range Type Note Age Body Brain BrainSD BrainCV N brain Correl RGC to brain CAST/Ei = CAS 153 13 373 31 8.4% 13 CAS32F1 75 18.7 458 25 5.5% 8 CAS32F2 37 15.7 424 26 6.2% 68 BXD32 119 24 435 19 4.3% 18 "t dist, 2-tailed, p<0.025 per tail)" Odd strains RGCs SEM SD N CV H or L 95%CI- 95%CI+ Type Body Brain TgN(Hbb-b1)83/LoGo "56,912" "±1,969" "±4,404" 6 7.7% I "52,093" "61,731" 100 NA NA B6C3Fe-a/a-rl/ "63,357" "±4,148" "±9,276" 6 14.6% I "53,206" "73,507" 106 NA NA Strain Status Sex Study Group Case_Mean_Axons Strain_Mean_Axons Strain_Axons_SE Strain_Axons_SD Strain_N Case_Mean_Axons Case_SEM Axons Sample_SEM Precision Nerve_Area Unmyelinated Std_Err_Unmyelinated Density Z_Score_RGC Z_Score_Brain RGCs JAX_Order_Type ID_number Side/level Strain_Brain_Weight DOB date_killed Age Body_weight Brain_weight Brain_weight_2 Cerebellum Retina_area Sex_Number Litter Note1 Note2 Necrosis DNA Mother Father Date_Photograhed Block Box_Slot Tissue_Score Hi_Mag Low_Mag Photos_By "Count_By, Checked_By" Area_of_Montage Negative_Area_Counted Sample_Area Coverage Photos_to_Cover_Nerve Samples Count_Sum Average_Axons_per_Photo Std_Dev_Axons_Photo Std_Err_Axons_Photo Photo 0 Photo 1 Photo 2 Photo 3 Photo 4 Photo 5 Photo 6 Photo 7 Photo 8 Photo 9 Photo 10 Photo 11 Photo 12 Photo 13 Photo 14 Photo 15 Photo 16 Photo 17 Photo 18 Photo 19 Photo 20 Photo 21 Photo 22 Photo 23 Photo 24 Photo 25 Photo 26 Photo 27 Photo 28 Photo 29 Photo 30 Photo 31 Photo 32 Photo 33 Photo 34 Photo 35 Photo 36 Photo 37 Photo 38 Photo 39 Photo 40 Photo 41 Photo 42 Photo 43 Photo 44 Photo 45 Photo 46 Photo 47 Photo 48 Photo 49 Photo 50 "set 2, 1" Criteria= 1 6 <50000 -0.2786 "=""=DR""" 1.1691 mean n 7.5 "59,467" "±3,687" "59,116" "±4,008" 6.92% =average of single estimates 310 23 571 #DIV/0! #DIV/0! #DIV/0! N mean SD SEM Precision harmonic 4.6 "±3,435" 5.70% =technical precision of single estimates 17 "rw=77 (58.8), rcs=96 (62.3), dr=44 (59.8), tk=129+24 (60.3 + 59.2), dt=18 (57.7)" 17 46428 3953 988 1.91% 0.5% "57,474" "1,766" final technical "±3,161" 5.24% =technical following replication "55,850" 2007.11905 58285.691 34570.3368 Conventions: Notes on status of data by row "1=in first studies, blank=not included in first studies, 2=0.00001, 3=0.00000001" "1=standard inbred, 2=BXD, 3=isogenic F1 hybrid, 4=wild, 5=mutants, 6=F2 progeny of F1 intercross, 8=backcross N1, 9=random bred or outcrossed" ID number is usually the date followed by a letter. Animals are perfused in A to Z sequence. Wrong equation "Average count for animal. If a nerve was recounted then this value is a variance weighted average (Bevington and Robinson 1992 Data Reduction and ErrorAnalysis, 2ed, p. 59). This value may average left and right nerves." "SEM of count. If a nerve was recounted then this value is a variance weighted SEM (Bevington and Robinson 1992 Data Reduction and ErrorAnalysis, 2ed, p. 59)." Estimated total population of myelinated and unmyelinated axons in the optic nerve. Standard error of the estimate based on the variation within the sample of micrographs. "Internal sample precision as a coefficient of variation. This value does not take into account external sources of inaccuracy, such as fixation, counting error, misidentification, etc." Nerve area in square microns. Estimate of the number of unmyelinated axons. If data are not shown they can be obtained from the raw counts in the micrographs columns. In these columns data for the two types are entered in the formula in this sequence = myelinated + unmyelinated. Standard error of the estimate of unmyelinated fibers based on the variation within the sample of micrographs. density of axons per 100 square microns Normal score for the ganglion cell population of a single case based upon the strain mean and standard deviation. Normal score for the brain weight of a single case based upon the strain mean and standard deviation. Direct estimate of ganglion cell number by Denny Rice from wholemounted HRP-reacted pairs of retinas. "In most cases right and left nerves are processed together. Therefore, the side of origin is usually lost." "date of birth in year, month, day format" "date killed in year, month, day format" "age in days, calculated or estimated" live body weight just prior to fixation "fixed brain weight measured within 2-7 days of fixation. Brains are blocked just behind the cerebellum. The caudal brain setem is discarded. Weighte almost always include the olfactory bulbs and usually the floculus, but not the pituitary." Data from Denny Rice based on processed HRP reacted retinal wholemounts. "1=Female, 0=Male" Arbitrary litter numbers used just to determine number of litters examined in each strain Incidence of necrotic fibers in the sample Notes on status of tissue taken for DNA extraction. This is work of Richelle Strom. Data that a sample was photographed. EM block number used to generate thin sections. There are usually two blocks per case from the right and left sides. Location of thin sections "4=great, 3=good, 2=ok, 1=poor. Poor cases have usually been discarded. However, they may have been retained in this database if brain weight data were available." The magnification of the images used to count axons. The magnification of the image used to measure the cross-sectional area of the optic nerve thin section. Initials of the person who sampled the nerve. "Initials of the person who first counted axons, followed by initials of the person who checked the count. Most cases were double or triple checked by Rob Williams." The corrected area of the cross-section of the thin section that was sampled. #DIV/0! " 5,877 " Strain Status Sex Study Group ID_Number Weighted_Strain_Mean Strain_Mean_Axons Strain_Axons_SE Strain_Axons_SD Strain_N Case_Mean_Axons Case_SEM Axons Sample_SEM Precision Nerve_Area Unmyelinated SEM_Unmyelinated Density Z_Score_RGC Z_Score_Brain RGCs JAX_Order_Type ID_number Side/level Strain_Brain_Weight DOB date_killed Age Body_weight Brain_weight Brain_weight_2 D11Mit1 Cerebellum Retina_area Sex_Number Litter Note1 Note2 Necrosis Tissue_DNA Mother Father Photo_Date Block Box_Slot Tissue_Score Hi_Mag Low_Mag Photos_By "Count_By, Checked_By" Area_of_Montage Negative_Area_Counted Sample_Area Coverage Photos_to_Cover_Nerve Samples Count_Sum Average_Axons_per_Photo Std_Dev_Axons_Photo Std_Err_Axons_Photo Weighting_A 1/Variance Even_Sample Odd_Sample %_diff Odd_vs_Even_Diff Photo 0 Photo 1 Photo 2 Photo 3 Photo 4 Photo 5 Photo 6 Photo 7 Photo 8 Photo 9 Photo 10 Photo 11 Photo 12 Photo 13 Photo 14 Photo 15 Photo 16 Photo 17 Photo 18 Photo 19 Photo 20 Photo 21 Photo 22 Photo 23 Photo 24 Photo 25 Photo 26 Photo 27 Photo 28 Photo 29 Photo 30 Photo 31 Photo 32 Photo 33 Photo 34 Photo 35 Photo 36 Photo 37 Photo 38 Photo 39 Photo 40 Photo 41 Photo 42 Photo 43 Photo 44 Photo 45 Photo 46 Photo 47 Photo 48 Photo 49 Photo 50 Photo 51 Photo 52 Photo 53 Photo 54 Photo 55 Photo 56 Photo 57 Photo 58 Photo 59 Photo 60 Photo 61 Photo 62 Photo 63 Photo 64 Photo 65 Photo 66 Photo 67 Photo 68 Photo 69 Photo 70 Photo 71 Photo 72 Photo 73 Photo 74 Photo 75 Photo 76 Photo 77 Photo 78 Photo 79 Photo 80 Photo 81 Photo 82 Photo 83 Photo 84 Photo 85 Photo 86 Photo 87 Photo 88 Photo 89 Photo 90 Photo 91 Photo 92 Photo 93 Photo 94 Photo 95 Photo 96 Photo 97 Photo 98 Photo 99 Photo 100 Photo 101 Photo 102 Photo 103 Photo 104 Photo 105 Photo 106 Photo 107 Photo 108 Photo 109 Photo 110 Photo 111 Photo 112 Photo 113 Photo 114 Photo 115 Photo 116 Photo 117 Photo 118 Photo 119 Photo 120 Photo 121 Photo 122 Photo 123 Photo 124 Photo 125 Photo 126 Photo 127 Photo 128 Photo 129 Photo 130 Photo 131 Photo 132 129/J F 1 1 121092C "62,384" "63,772" "±1,699" "±4,163" 7 "66,315" "±2,522" "66,315" "±2,522" 3.8% "60,663" "7,909" "1,156" 109.32 0.611 "61,344 " JR000690 R 407 921111 921211 30 16.5 NA 14.1 1 1 Albino. 15% unmyelinated. Low mag calibration taken from other case shot at same time. 1 necrotic on 0033 12-Jan-93 A4 "Box 21, Slot QÐ4" 4 "13,198 x" 202 x DR DR and RW "2,475 mm^2" "5,094 mm^2" 29.2 µm^2 1.40% 2075 29 927 32.0 ±6.43 ±1.22 1.0795E-02 1.6279E-07 71611 69036 96.4% " 2,575 " 39 30 33 41 37 32 40 35 28 38 32 32 38 29 23 31 35 24 21 34 15 29 26 32 41 29 37 40 26 129/J original F 1 1 062892C better "57,185" "±1,759" "57,292" "±2,120" 3.7% "91,289" 62.76 -1.582 "58,479 " JR000690 L 920629 200 26.0 440 18.1 1 2 Some drift during photography. Poor image quality. Calibration grating used instead of standard grid. few unmylinated 1 or 2 necrotic in series 18-Sep-92 D4 "Box 17, Slot Q-2" 4 "11,073 x" 197 x DR DR and RW "3,540 mm^2" "5,094 mm^2" 41.5 µm^2 1.91% 2198 42 1095 26.1 ±6.18 ±0.96 1.8937E-02 3.3115E-07 59437 59983 100.9% 545 20 23 21 24 28 21 27 21 16 29 26 22 32 33 18 27 16 23 24 35 25 31 22 40 33 21 28 30 23 24 42 25 22 27 30 23 29 25 38 14 26 31 129/J replicate 0.00001 062892C "56,950" "±3,150" 5.5% "97,294" 58.53 Replicate of same section. 1 or 2 19-Apr-95 D4 "Box 17, Slot Q-2" 4 "10,845 x" 192 x RW RW and RW "3,599 mm^2" "5,270 mm^2" 44.8 µm^2 1.01% 2171 22 577 26.2 ±6.65 ±1.45 13 26 30 43 33 30 23 27 16 24 26 34 28 34 20 25 27 28 22 27 24 17 129/J M 1 1 062192B "65,650" "±5,869" "65,650" "±5,869" 8.9% "47,214" 139.05 0.451 "72,031 " JR000690 L 920622 200 23.0 401 17.3 0 2 Denny notes that this mouse looks really beat up. Mouse is a retired breeder. Killed at the Jackson Lab. ">50% unmyelinated. Yes, about half are unmyelinated. Section is presumably close to lamina cribrosa. Excellent fixation. Count is very good" 1 or 2 necrotic in series 18-Sep-92 D3 "Box 17, Slot O-4" 4 "20,611 x" 250 x DR DR and RW "2,949 mm^2" "5,138 mm^2" 12.1 µm^2 1.26% 3904 49 824 16.8 ±10.42 ±1.50 1.9456E-03 2.9636E-08 65046 66454 102.2% " 1,407 " 12 24 33 21 2 3 21 11 15 8 20 2 48 18 33 18 1 13 13 0 0 17 11 19 not available 22 11 19 36 18 13 18 20 22 18 14 11 21 26 21 6 20 16 15 38 15 3 31 1 26 129/J M 1 1 082094E "59,344" "±2,831" "59,344" "±2,831" 4.8% "65,474" 90.64 -1.064 JR000690 ? 940620 940820 61 22.0 394 0 3 none 31-Oct-94 10 "Box 20, Slot D-2" 3 "13,075 x" 191 x DR RW and RW "2,389 mm^2" "5,418 mm^2" 31.7 µm^2 1.06% 2066 22 632 28.7 ±6.28 ±1.37 7.7559E-03 1.3069E-07 55944 60449 108.1% " 4,506 " 22 18 32 38 26 32 24 24 33 30 33 32 26 28 34 26 28 28 35 34 12 37 129/J M 1 1 112194D "62,805" "±3,087" "62,805" "±3,087" 4.9% "56,655" 110.85 -0.232 JR000690 ? 941017 941121 35 17.0 395 0 4 Albino none 2-Feb-95 8 "Box 37, Slot M-2" 4 "13,156 x" 193 x TK RCS and RW "2,120 mm^2" "5,418 mm^2" 31.3 µm^2 1.11% 1810 20 694 34.7 ±7.43 ±1.71 6.9371E-03 1.1046E-07 61004 64252 105.3% " 3,249 " 38 48 25 28 36 28 41 44 48 26 38 42 26 34 37 30 39 28 28 30 129/J M 1 1 112194E "66,697" "±4,710" "66,697" "±4,710" 7.1% "69,246" 96.32 0.703 JR000690 ? 941017 941121 35 16.5 398 0 4 Albino none 2-Feb-95 9 "Box 37, Slot N-2" 4 "13,156 x" 194 x TK RCS and RW "2,609 mm^2" "5,418 mm^2" 31.3 µm^2 0.90% 2212 20 603 30.2 ±9.28 ±2.13 3.1646E-03 4.7447E-08 70862 63087 89.0% " 7,775 " 43 26 27 38 14 29 35 31 28 41 29 17 37 36 45 11 29 35 32 20 129/J original F 1 1 112194F "68,409" "±2,365" "68,154" "±3,329" 4.9% "53,081" 128.39 1.114 JR000690 ? 941017 941121 35 14.8 390 1 4 Albino none 3-Feb-95 12 "Box 37, Slot O-2" 4 "13,156 x" 194 x TK RW and RW "1,995 mm^2" "5,418 mm^2" 31.3 µm^2 1.24% 1696 21 844 40.2 ±8.78 ±1.96 1.2840E-02 1.8769E-07 72678 64218 88.4% " 8,460 " 27 54 41 40 41 43 55 36 37 37 31 44 48 45 41 55 44 21 37 30 37 129/J replicate 0.00001 112194F "68,669" "±3,361" 4.9% "53,678" 127.93 Replicate of a different section none 2-May-95 12 "Box 37, Slot O-4" 4 "12,909 x" 247 x RW RW and RW "3,265 mm^2" "5,270 mm^2" 31.6 µm^2 1.30% 1697 22 890 40.5 ±9.07 ±1.98 50 35 44 38 23 50 60 38 48 36 34 38 53 30 47 40 31 42 52 36 32 33 129/J not countable M 082094D JR000690 940620 940820 61 21.0 422 3 Fixation too poor to count. Good data on brain weight. 129/J not countable M 082094F JR000690 940620 940820 61 20.0 404 3 Fixation too poor to count. Good data on brain weight. 129/J not countable M 082094G JR000690 940620 940820 61 21.0 411 3 Fixation too poor to count. Good data on brain weight. 129/J not countable M 082094H JR000690 940620 940820 61 22.5 415 3 Fixation too poor to count. Good data on brain weight. Brain reweighed by Toya at 0.4147 gm. 129.AKR-bs/bs 5 070993C "43,466" "±2,531" "43,466" "±2,531" 5.8% "71,948" 60.41 930709 not pefused here dup Blind sterile homozygous mutant on a 129.AKR congenic hybrid. 2 necrotic fibers 29-Nov-94 "12,931 x" 190 x DR RW and DR "2,588 mm^2" "5,418 mm^2" 32.4 µm^2 1.17% 2220 26 509 19.6 ±5.70 ±1.14 7.0552E-03 1.6231E-07 39793 47137 118.5% " 7,344 " 19 16 16 15 18 18 21 22 22 18 26 15 31 7 26 19 25 16 18 29 23 21 24 22 7 15 129.AKR-bs/+ F 081094F 940328 940810 135 17.0 428 Normal heterozygote carrier of blind sterile on a 129.AKR congenic hybrid. Injected with HRP. Good perfusion 129.AKR-bs/+ M 081094G 940709 940810 32 14.0 433 1 Normal heterozygote carrier of blind sterile on a 129.AKR congenic hybrid. Injected with HRP. Good perfusion 129.AKR-bs/+ F 081094H 6.7% 940709 940810 32 14.0 428 1 Normal heterozygote carrier of blind sterile on a 129.AKR congenic hybrid. Injected with HRP. Poor perfusion 129BL6F1-dy/dy F 3 071593E "68,757" "±6,392" "68,757" "±6,392" 9.3% "126,587" 54.32 930525 930715 51 1 1 Dystonia muscularis carried on 129/ReJ C57BL/6J F1 A3 "Box 28, Slot L-9" 3 "13,077 x" 193 x TK RW and RW "4,730 mm^2" "5,418 mm^2" 31.7 µm^2 0.60% 3996 24 413 17.2 ±7.67 ±1.60 1.7559E-03 2.5537E-08 81241 56270 69.3% " 24,972 " 17 22 5 17 13 28 29 21 14 16 3 21 10 20 19 37 14 17 12 14 8 13 25 18 129BL6F1-dy/dy M 3 071593F "71,929" "±6,537" "71,929" "±6,537" 9.1% "103,832" 69.27 930525 930715 51 0 1 Dystonia muscularis carried on 129/ReJ C57BL/6J F1 B1 "Box 28, Slot M-2" 2 "13,077 x" 193 x TK RW and RW "3,880 mm^2" "5,418 mm^2" 31.7 µm^2 0.58% 3277 19 417 21.9 ±8.46 ±1.99 1.7768E-03 2.4703E-08 73083 70643 96.7% " 2,440 " 29 21 11 20 18 27 13 26 40 18 20 17 13 35 30 8 31 22 18 A/J score necrotic F 1 1 062892F "51,238" "±1,362" "±3,602" 8 "49,303" "±2,752" "49,303" "±2,752" 5.6% "61,583" 80.06 -0.537 "44,779 " JR000646 JAX307401 R 395 920629 200 27.0 401 15.6 1 3 "This is the re-shoot. A retired breeder from the Research facility at Jackson Lab, room 13, Sect 40. Killed at the Jackson Lab." Few unmyelinated 15-Mar-93 A3 "Box 17, Slot B-2" 3 "12,995 x" 192 x DR DR and RW "2,279 mm^2" "5,313 mm^2" 31.5 µm^2 1.38% 1958 27 680 25.2 ±7.17 ±1.41 6.7597E-03 1.3711E-07 51809 56076 108.2% " 4,266 " 31 27 33 16 25 12 28 19 19 40 39 21 23 36 33 25 12 26 21 27 23 21 31 24 23 20 25 A/J M 1 1 062192D worse "49,533" "±2,599" "49,533" "±2,599" 5.2% "62,036" 79.84 -0.473 JR000646 ? 920621 200 26.0 420 0 3 "Perfusion went badly. Recounted Dec 94 by Rob, but probably still a slight undercount. Keep data. A retired breeder from the Research facility at Jackson Lab, room 13, Sect 40. Killed at the Jackson Lab." Perhaps 1 necrotic 30-Oct-94 A2 "Box 20, Slot A-1" "13,080 x" 193 x DR RW and RW and RW "2,312 mm^2" "5,418 mm^2" 31.7 µm^2 1.07% 1959 21 531 25.3 ±5.93 ±1.33 7.6977E-03 1.5541E-07 50796 48132 94.8% " 2,664 " 21 31 20 23 31 26 30 32 24 32 26 22 22 15 19 31 37 25 14 23 27 A/J score necrotic M 1 1 062192C "49,755" "±3,144" "49,755" "±3,144" 6.3% "56,209" 88.52 -0.411 "61,765 " JR000646 JAX307401 R 920622 200 26.0 NA 14.4 0 3 "A retired breeder. A retired breeder from the Research facility at Jackson Lab, room 13, Sect 40. Killed at the Jackson Lab." "<1% unmyelinated. Brain cut, not weighed" 28-Sep-93 D5 "Box 17, Slot I-1" "14,025 x" 256 x DR DR and RW "3,692 mm^2" "5,103 mm^2" 25.9 µm^2 1.29% 2167 28 643 23.0 ±7.54 ±1.45 5.2202E-03 1.0492E-07 55366 54173 97.8% " 1,192 " 34 30 36 30 24 19 25 24 29 23 26 8 18 30 18 17 33 33 19 12 23 20 15 20 7 25 18 27 A/J replicate F 1 1 021493F "51,298" "±2,335" "49,045" "±2,562" 5.2% "51,227" 95.74 0.017 "57,918 " JR000646 L 930215 35 17.0 357 12.2 1 4 Third sample by Denny on the original thin section also counted the first time. B5 "Box 20A, Slot I-1" 3 "13,011 x" 194 x DR DR and RW "1,922 mm^2" "5,418 mm^2" 32.0 µm^2 1.75% 1601 28 858 30.6 ±8.32 ±1.60 9.7566E-03 1.9019E-07 48064 50830 105.8% " 2,766 " 43 29 39 34 38 18 32 33 29 10 24 30 24 33 25 27 13 30 38 28 31 35 31 46 38 24 36 40 A/J replicate 2 0.00001 021493F "46,566" "±5,671" 12.2% "47,313" 98.42 JR000646 L 930215 dup Second sample by Rob of an adjacent thin section. Low contrast. "A completely independent analysis of an adjacent thin section on the same grid by Rob. Focus on some micrographs is poor, but count is good." 26-Aug-94 B5 "Box 20A, Slot I-1" "12,834 x" 246 x RW DR and RW "2,856 mm^2" "5,418 mm^2" 32.9 µm^2 1.11% 1438 16 518 32.4 ±15.27 ±3.94 52720 51950 98.5% 770 30 7 46 29 46 28 15 55 20 40 15 23 19 44 45 56 A/J original 0.00001 021493F "56,667" "±3,309" 5.8% "51,642" 109.73 L First sample by Denny. Recalibrated nerve cross-sectional area. From the Jackson Lab production colony. B5 "Box 20A, Slot I-1" "13,098 x" 245 x DR DR and RW "3,090 mm^2" "5,324 mm^2" 31.0 µm^2 1.26% 1664 21 715 34.0 ±8.89 ±1.99 26.0 35.0 39.0 27.0 32.0 25.0 46.0 48.0 28.0 25.0 30.0 31.0 25.0 56.0 35.0 32.0 31.0 38.0 44.0 22.0 40.0 A/J original M 1 1 091394G "56,720" "±2,005" "55,542" "±2,770" 5.0% "53,730" 103.37 1.522 JR000646 L 940914 100 24.0 360 0 2 Albino. From The Jackson Laboratory production colony. 26-Aug-94 15 or 16 "Box 30, Slot L-3" 3 "12,968 x" 187 x DR DR and RW "1,878 mm^2" "5,418 mm^2" 32.2 µm^2 1.38% 1668 23 766 33.3 ±7.79 ±1.66 1.4754E-02 2.6012E-07 57951 52910 91.3% " 5,041 " 38 36 48 31 35 18 27 16 26 41 37 39 26 34 46 35 29 30 36 36 41 33 28 A/J replicate 0.00001 091394G "58,018" "±2,906" 5.0% "53,675" 108.09 JR000646 L 940914 dup Redone again by Rob 19-Oct-94 15 or 16 "Box 30, Slot L-3" "16,280 x" 227 x RW RW and RW "2,761 mm^2" "5,418 mm^2" 20.4 µm^2 0.80% 2626 21 464 22.1 ±4.95 ±1.11 56321 59339 105.4% " 3,018 " 24 25 double exposure 26 26 26 32 25 22 15 28 25 23 21 14 24 22 19 21 18 15 13 A/J M 1 1 100494M "50,530" "±2,830" "50,530" "±2,830" 5.6% "58,480" 86.41 -0.196 JR000646 ? 941004 52 21.5 418 0 1 Albino. From The Jackson Laboratory production colony. none 2-Dec-94 6 "Box 34, Slot B-2" 4 "13,037 x" 193 x TK RW and RW "2,179 mm^2" "5,418 mm^2" 31.9 µm^2 1.20% 1834 22 606 27.5 ±7.07 ±1.54 6.6104E-03 1.3082E-07 52196 48861 93.6% " 3,335 " 30 28 34 16 30 34 27 16 23 25 26 21 11 31 29 35 39 22 34 30 30 35 A/J original M 1 1 100494N "56,449" "±4,946" "56,449" "±4,946" 8.8% "57,427" 98.30 1.447 JR000646 ? 941004 52 22.5 431 0 1 Albino. From The Jackson Laboratory production colony. 1 necrotic on 0033 4-Dec-94 8 "Box 34, Slot C-3" 4 "13,055 x" 193 x TK RW and RW "2,139 mm^2" "5,418 mm^2" 31.8 µm^2 0.89% 1806 16 500 31.3 ±10.61 ±2.74 2.4611E-03 4.3599E-08 59383 53513 90.1% " 5,871 " 44 43 39 34 28 27 25 23 35 40 48 26 33 32 11 12 A/J please replicate 1E-08 100494N A/J original F 1 1 100494O "46,312" "±2,059" "46,312" "±2,059" 4.4% "51,333" 90.22 -1.367 JR000646 ? 941004 52 13.8 376 1 1 Albino. From The Jackson Laboratory production colony. All values triple-checked. none 4-Dec-94 9 "Box 34, Slot D-2" 4 "13,055 x" 193 x TK RW and RW "1,912 mm^2" "5,418 mm^2" 31.8 µm^2 1.36% 1615 22 631 28.7 ±5.84 ±1.27 1.1449E-02 2.4722E-07 48146 44476 92.4% " 3,670 " 28 36 27 20 28 29 34 32 24 30 18 22 26 23 25 35 26 32 30 27 37 42 A/J please replicate 1E-08 100494O A/J ordered March 95 for breeding F breeder A/J ordered March 95 for breeding F breeder AKR/J original F 1 1 092092A "62,788" ±890 "±1,990" 6 "63,233" "±2,341" "66,131" "±4,193" 6.3% "68,295" 96.83 0.224 JR000648 R GET WEIGHTS 920806 920920 45 NA 428 1 1 Counted using two separate counting frames. Data are taken from the second count using the standard frame. <15% unmyelinated none 4-Nov-92 A3 "Box 14, Slot L-1" 3 "13,250 x" 255 x DR RW and RW "4,450 mm^2" "5,270 mm^2" 30.0 µm^2 1.32% 2275 30 872 29.1 ±9.92 ±1.84 1.1935E-02 1.8875E-07 64025 80538 125.8% " 16,513 " 31 28 30 edge blank 27 25 34 28 38 27 21 40 28 39 18 5 21 25 41 46 43 41 19 37 19 28 15 23 18 45 32 AKR/J replicate 0.00001 092092A "63,588" "±2,822" 4.4% "72,165" 88.11 This is the second interleave of the high resolution count by Richelle. The lower density is not due to any difficulty in counting or resolving axons. none 15-Mar-95 A3 "Box 14, Slot L-3" "10,913 x" 193 x RCS RCS and RCS "2,697 mm^2" "5,418 mm^2" 45.5 µm^2 3.66% 1586 58 2325 40.1 ±13.43 ±1.78 41 22 30 24 6 33 28 7 45 43 50 45 44 48 45 45 54 27 72 60 52 47 42 30 45 25 31 20 cal cal 46 19 50 27 40 43 62 56 58 57 37 35 36 24 45 57 48 46 57 49 40 22 25 30 47 43 42 42 49 32 AKR/J replicate 2 0.00001 092092A "62,091" "±2,334" 3.8% "74,419" 83.43 This is the first interleave of the high resolution count with about 60 sample photographs by Rob none 15-Mar-95 A3 "Box 14, Slot L-3" "10,842 x" 191 x RW RW and RCS "2,711 mm^2" "5,418 mm^2" 46.1 µm^2 3.65% 1615 59 2269 38.5 ±11.01 ±1.45 38 36 36 41 38 31 37 43 34 39 52 49 41 42 59 34 40 21 60 46 47 36 33 34 27 32 36 33 23 44 10 59 46 57 26 46 61 44 35 48 34 39 48 46 48 55 34 29 27 23 37 15 38 23 41 31 50 33 24 AKR/J original F 1 1 092092B "60,063" "±1,913" "62,660" "±4,625" 7.4% "80,965" 77.39 -1.370 "53,457 " JR000648 R 920806 920920 45 NA 427 14.7 1 1 Check birth date "<5% unmyelinated. Calibrations redo by Rob, March 1995." none 4-Nov-92 A6 "Box 14, Slot O-2" 3 "13,312 x" 255 x DR DR and RW "5,256 mm^2" "5,012 mm^2" 28.3 µm^2 1.26% 2863 36 788 21.9 ±9.56 ±1.62 1.6887E-02 2.8115E-07 62402 68743 110.2% " 6,341 " 21 24 24 7 13 0 22 34 21 26 28 27 26 25 31 28 edge 29 28 25 28 0 30 23 31 27 26 15 18 4 4 22 24 8 edge 33 36 20 AKR/J replicate 0.00001 092092B "60,024" "±2,101" 3.5% "84,177" 71.31 "This is the first interleaved set of about 60 micrographs (0.5 rotations right to left, 1 rotation, between rows). Same section as the second interleaved set below. Different thin section from the original estimate." none 17-Mar-95 A6 "Box 14, M-4" "10,996 x" 248 x RW RW and RW "5,193 mm^2" "5,418 mm^2" 44.8 µm^2 3.46% 1878 65 2077 32.0 ±8.95 ±1.12 cal 21 19 33 29 30 22 29 52 37 47 31 41 25 36 32 38 39 23 39 31 42 35 42 37 33 47 40 27 21 21 38 42 47 35 24 40 12 40 35 35 18 33 14 24 30 30 27 31 36 43 31 29 37 26 12 23 40 34 34 10 36 33 32 38 29 AKR/J replicate 2 0.00001 092092B "58,384" "±3,458" 5.9% "82,661" 70.63 "This is the second interleaved set of about 30 micrographs (0.5 rotations right to left, 1 rotation, between rows). Series ends at about micrograph #103" none 17-Mar-95 A6 "Box 14, M-4" "10,991 x" 248 x RW RW and RW "5,083 mm^2" "5,418 mm^2" 44.8 µm^2 1.68% 1843 31 982 31.7 ±10.28 ±1.88 39 25 10 20 33 35 37 25 31 28 32 44 44 36 43 37 27 45 36 11 23 34 46 33 29 22 40 30 36 44 7 AKR/J replicate 3 0.00001 092092B "60,325" "±3,079" 5.1% "83,650" 72.12 "This is the third interleaved set of 29 micrographs (0.5 rotations right to left, 1 rotation, between rows). Series ends at about micrograph #132." none 17-Mar-95 A6 "Box 14, M-4" "10,968 x" 247 x RW RW and RW "5,099 mm^2" "5,418 mm^2" 45.0 µm^2 1.56% 1857 29 942 32.5 ±8.77 ±1.66 14 28 25 34 33 39 33 23 32 26 39 33 27 35 31 29 32 49 34 47 42 21 39 28 27 40 30 53 19 AKR/J get body weight F 1 1 021493G "65,143" "±2,754" "69,029" "±4,536" 6.6% "69,410" "15,277" "1,971" 99.45 1.184 "61,755 " JR000648 R 930215 35 NA 465 14.7 1 2 Low mag cal and photomontage redone by Rob. 4-6 weeks 9-Mar-93 C2 "Box 20A, Slot J-2" 4 "13,123 x" 245 x DR and RW DR and RW "4,176 mm^2" "5,330 mm^2" 30.9 µm^2 1.20% 2243 27 831 30.8 ±10.31 ±2.02 8.9206E-03 1.3694E-07 63758 74701 117.2% " 10,943 " 32 24 30 9 25 34 34 4 19 26 24 44 21 31 47 34 41 49 31 33 34 43 35 38 28 35 26 AKR/J 0.00001 021493G "65,893" "±3,466" 5.3% "63,309" 104.08 JR000648 R 930215 dup dup "Again, a complete new set of data from same slot but a third thin section. Restained to improve contrast. " none 7-Mar-95 C2 "Box 20A, Slot J-2" "13,054 x" 194 x RW RW and RW "2,372 mm^2" "5,418 mm^2" 31.8 µm^2 1.10% 1991 22 728 33.1 ±7.98 ±1.74 62539 68666 109.8% " 6,128 " 29 42 52 26 33 40 36 34 28 28 31 33 35 40 17 39 41 26 22 39 23 34 AKR/J 0.00001 021493G "61,273" "±3,924" 6.4% "67,178" 91.21 JR000648 R 930215 dup dup "A second set of data from same slot but a second thin section. Low contrast, but good tissue. High mag calibration is asymmetric. This probably account for some of the variation." none 15-Aug-94 C2 "Box 20A, Slot J-2" "12,897 x" 246 x RW RW and RW "4,067 mm^2" "5,418 mm^2" 32.6 µm^2 1.16% 2062 24 713 29.7 ±9.12 ±1.90 56029 66513 118.7% " 10,484 " 33 40 38 39 29 36 32 39 24 24 29 26 28 44 26 14 34 28 35 29 8 31 37 10 AKR/J M 1 1 040494I "64,683" "±4,484" "64,683" "±4,484" 6.9% "119,298" 54.22 0.952 940904 100 NA 479 0 3 15-Sep-94 "Box 28, Slot N-1" "13,007 x" 247 x RW RW and RW "7,256 mm^2" "5,418 mm^2" 32.0 µm^2 0.59% 3725 22 382 17.4 ±5.52 ±1.20 3.3695E-03 5.2093E-08 70733 63030 89.1% " 7,704 " 9 17 15 9 15 15 24 23 18 16 14 12 28 22 13 20 13 27 12 16 19 25 AKR/J original F 1 1 040494J "61,064" "±2,334" "65,142" "±4,411" 6.8% "99,768" 65.29 -0.867 940904 100 NA 496 1 3 15-Sep-94 15/16 "Box 28, Slot O-2" 4 "13,107 x" 247 x RW RW and RW "6,102 mm^2" "5,418 mm^2" 31.5 µm^2 0.70% 3164 22 453 20.6 ±6.39 ±1.39 1.1742E-02 1.9229E-07 68715 67215 97.8% " 1,500 " 19 19 21 20 12 38 25 19 17 5 16 24 21 14 26 19 18 27 23 23 26 21 AKR/J replicate 0.00001 040494J "59,325" "±2,751" 4.6% "100,830" 58.84 Replicate of different section started by RCS and finished next day by Rob and Toya. This is the first interleaved set of about 80 micrographs none 18-Mar-95 15/16 "Box 28, Slot O-4" "10,962 x" 193 x RCS and RW RW and RW "3,757 mm^2" "5,418 mm^2" 45.1 µm^2 3.49% 2236 78 2069 26.5 ±10.79 ±1.23 12 17 13 11 22 13 7 17 16 19 19 19 20 22 9 13 27 28 39 38 26 29 10 9 10 18 26 45 13 24 49 35 29 27 19 21 24 13 43 30 32 49 53 28 29 28 34 24 30 39 22 14 35 43 30 38 28 29 17 27 16 29 47 24 28 40 25 38 19 36 29 23 32 37 46 30 33 27 AKR/J replicate 2 0.00001 040494J "61,430" "±4,259" 6.9% "100,741" 60.98 Replicate of same section (second interleaved set) of about 40 micrographs by Toya. none 18-Mar-95 15/16 "Box 28, Slot O-4" "10,966 x" 193 x TK RW and RW "3,764 mm^2" "5,418 mm^2" 45.1 µm^2 1.79% 2236 40 1099 27.5 ±11.90 ±1.91 10 8 13 14 29 30 32 16 14 50 45 35 17 23 18 20 24 54 44 33 29 4 39 42 50 26 28 31 32 31 22 37 31 22 29 30 32 23 14 18 AKR/J F 1 1 040494K "62,543" "±4,668" "62,543" "±4,668" 7.5% "96,249" 64.98 -0.123 940904 100 NA 471 1 3 15-Sep-94 "Box 28, Slot P-1" 4 "13,046 x" 247 x RW RW and RW "5,886 mm^2" "5,418 mm^2" 31.8 µm^2 0.63% 3024 19 393 20.7 ±6.55 ±1.54 3.0300E-03 4.8447E-08 71121 59910 84.2% " 11,211 " 11 20 23 25 20 26 30 16 9 26 9 16 21 19 24 22 25 33 18 B6C3Fe-+/rl F 1 5 910311H "63,357" "±4,148" "±9,276" 6 "69,879" "±2,440" "69,879" "±2,440" 3.5% "77,914" 89.69 0.703 JR0235 L 901024 910311 138 No data 1 1 "A reeler heterozygote. All calibrations etc redone by Rob, Dec 1994." 1-May-91 B2 "Box 11, Slot O-2" 3 "11,175 x" 391 x DT 4-30-91 DT and DT and RW "11,932 mm^2" "5,382 mm^2" 43.1 µm^2 1.88% 1808 34 1314 38.6 ±7.75 ±1.35 1.2089E-02 1.7300E-07 69664 70089 100.6% 425 42 42 25 48 33 31 30 40 38 44 46 42 47 29 33 43 29 41 38 39 36 39 56 34 46 39 44 39 42 46 52 32 22 27 B6C3Fe-rl/rl F 1 5 900607.1 "55,038" "±2,184" "55,038" "±2,184" 4.0% "79,505" 69.23 -0.897 JR0235 Reeler 1 "L, near chiasm" 900607 100 No data 1 2 "Case 2503. Reeler homozygote. All calibrations and areas redone by Rob, Dec 94." Reeler homozygote. 6-Mar-91 B4 "Box 11, Slot C-1" 3 "11,037 x" 385 x DT 2-14-91 DT and RW "11,767 mm^2" "5,250 mm^2" 43.1 µm^2 1.95% 1845 36 1074 29.8 ±7.00 ±1.18 1.1867E-02 2.1562E-07 56676 53396 94.2% " 3,280 " 28 20 32 29 21 23 26 22 31 21 30 34 25 27 26 32 20 28 33 26 29 49 35 35 19 32 36 38 25 36 25 29 46 41 34 31 B6C3Fe-rl/rl original F 1 5 900607.2 "58,139" "±1,775" "53,447" "±5,089" 9.5% "79,044" 67.62 -0.563 JR0235 Reeler #2 "L, near lamina cribrosa" 900607 100 No data 1 2 "Done by Denny at 12,000 on section adjacent to that used by Daphne. Good data. All calibrations and areas by Rob. Daphne's original count was about 68,000, but this high value due to miscalibration. " All of these reeler animals from Dan's colony at UT Memphis. 26-Sep-94 C2 "Box 12, Slot Q-2" 2 "13,003 x" 194 x "DR, Sept 25, 1994" DR and RW "2,962 mm^2" "5,418 mm^2" 32.0 µm^2 0.85% 2467 21 455 21.7 ±9.23 ±2.06 1.9377E-02 3.3329E-07 59202 48213 81.4% " 10,988 " 37 22 18 3 8 29 22 20 18 33 23 19 12 21 5 27 23 29 26 37 23 B6C3Fe-rl/rl replicate 0.00001 900607.2 "54,297" "±1,894" 3.5% "76,202" 71.25 JR0235 Reeler #2 "L, near lamina cribrosa" 900607 No data dup "Done again by Denny at 12,000 on same thin section used by Daphne, but contrast on Denny's negatives is quite low. Count seems fine to Rob. All calibrations and areas by Rob." Reeler homozygote. Very low contrast negatives. None 31-Dec-94 C2 "Box 12, Slot Q-2" "13,219 x" 194 x "DR on Dec 29, 1994" RW and RW "2,879 mm^2" "5,418 mm^2" 31.0 µm^2 0.90% 2458 22 486 22.1 ±3.53 ±0.77 55412 53178 96.0% " 2,234 " 26 30 18 19 23 22 19 18 25 21 22 26 24 24 20 20 17 20 21 29 23 19 B6C3Fe-rl/rl relicate 2 0.00001 900607.2 "53,658" "±3,052" 5.7% "73,911" 72.60 JR0235 Reeler #2 "L, near lamina cribrosa" 900607 No data dup Done yet again by Rob on middle thin section. Grid was restained first to improve contrast. Good count despite marginal fixation. 9-Mar-95 C2 "Box 12, Slot Q-2" "13,029 x" 194 x RW RW and RW "2,770 mm^2" "5,418 mm^2" 31.9 µm^2 1.25% 2316 29 672 23.2 ±6.97 ±1.32 19 26 26 25 25 20 19 19 11 25 24 29 18 22 22 29 3 19 24 34 25 24 27 29 31 11 25 23 38 B6C3Fe-rl/rl Right 0.00001 900607.2 "62,219" "±3,032" 4.9% "83,376" 74.63 JR0235 Reeler #2 "R, near chiasm" 900607 No data dup This is the right optic nerve near the optic chiasm None 31-Dec-94 D2 "Box 2, Slot I-1" "13,219 x" 194 x "DR on Dec 29, 1994" RW and RW "3,150 mm^2" "5,418 mm^2" 31.0 µm^2 0.82% 2689 22 509 23.1 ±5.17 ±1.13 65273 59161 90.6% " 6,112 " 19 16 22 23 23 23 29 19 19 19 16 27 33 19 17 27 27 23 35 22 27 24 B6C3Fe-rl/rl needs to be replicated F 1 5 900607.4 "78,574" "±4,331" "78,574" "±4,331" 5.5% "79,974" 98.25 1.641 JR0235 Reeler #4 R 900607 100 No data 1 2 "Redone Oct 94 by Rob at higher magnification to improve count. Initial count was 62,729. Poor images. Area of nerve in 1st set was 69385 mu2. Too low." Reeler homozygote. Section mid-orbital. Tiny mama Reeler #4 24-Oct-94 A2 "Box 3, Slot F-10" 2 "16,322 x" 222 x RW RW and RW "3,943 mm^2" "5,355 mm^2" 20.1 µm^2 0.50% 3978 20 395 19.8 ±4.74 ±1.09 4.4101E-03 5.6127E-08 81158 75986 93.6% " 5,172 " 26 20 17 21 20 19 27 15 15 19 13 31 20 21 23 20 12 15 18 23 B6C3Fe-rl/rl F 1 5 910311F "62,863" "±1,964" "62,863" "±1,964" 3.1% "68,868" 91.28 -0.053 JR0235 R 910311 100 No data 1 3 Case 2562. Area of nerve and photo double checked. All calibrations redone by Rob Dec 94. Reeler homozygote. 3-Apr-91 D4 "Box 11, Slot G-1" 2 "10,002 x" 360 x DT 4-1-91 DT and DT "8,940 mm^2" "5,250 mm^2" 52.5 µm^2 2.44% 1312 32 1533 47.9 ±8.33 ±1.50 1.6823E-02 2.6762E-07 61209 65362 106.8% " 4,154 " 57 51 56 63 48 42 41 edge 41 53 42 40 40 51 47 43 39 51 30 56 47 47 61 59 40 40 43 51 57 60 51 33 53 B6C3Fe-rl/rl F 1 5 910311G "55,648" "±3,058" "55,648" "±3,058" 5.5% "67,182" 82.83 -0.831 JR0235 L 910311 100 No data 1 3 "Older than 100 days. May be a slight undercount due to difficulty counting unmyelinated fibers. All calibrations redone by Rob, Dec 94." Reeler homozygote. 29-Dec-94 A4 "Box 12, Slot S-2" 2 "11,150 x" 376 x DT 5-14-91 DT and DT and RW "9,489 mm^2" "5,408 mm^2" 43.5 µm^2 1.88% 1544 29 1045 36.0 ±10.48 ±1.98 6.1617E-03 1.1073E-07 52601 59395 112.9% " 6,793 " 43 38 45 44 37 42 edge 34 55 45 39 20 43 16 26 35 52 37 36 19 31 20 25 28 43 57 38 37 30 30 B6AxCF1/JGo original F 1 3 120594I "64,854" "±2,408" "±5,898" 7 "59,256" "±2,721" "58,994" "±3,043" 5.2% "78,130" 75.51 -0.949 ? 468 941205 35 15.8 440 1 1 "Case 19. C57BL/6J female # 1 (64,245) or 2 (59,267) by BALB/c male #7" Hybrids generated at UT Memphis using Jackson Lab production stock 25-Jan-95 20 "Box 36, Slot O-2" 4 "13,189 x" 193 x RCS RW and RW "2,919 mm^2" "5,418 mm^2" 31.1 µm^2 1.08% 2508 27 635 23.5 ±6.19 ±1.21 8.3141E-03 1.4031E-07 56975 61165 107.4% " 4,190 " 26 30 28 11 22 28 20 30 16 23 13 20 17 20 32 29 17 16 24 25 25 34 22 19 26 32 30 B6AxCF1/JGo replicate 0.00001 120594I better "60,299" "±6,074" 10.1% "76,029" 79.31 Replicate on different section by Rob counted by Evan Williams 19-Apr-95 20 "Box 36, Slot O-2" "16,032 x" 192 x RW EGW and RW "2,813 mm^2" "5,270 mm^2" 20.5 µm^2 0.62% 3708 23 374 16.3 ±7.68 ±1.64 19 12 29 21 11 8 11 22 18 9 18 28 26 21 0 3 17 11 15 14 28 18 15 B6AxCF1/JGo F 1 3 120594J "63,580" "±4,408" "63,580" "±4,408" 6.9% "59,250" 107.31 -0.216 ? 941205 35 15.3 430 1 1 Case 20. C57BL/6J female # 1 or 2 by BALB/c male #7 Hybrids generated at UT Memphis using Jackson Lab stock 17-Jan-95 2 "Box 35, Slot N-1" 3 "18,024 x" 196 x TK RW and RW "2,267 mm^2" "5,418 mm^2" 16.7 µm^2 1.35% 3553 48 859 17.9 ±8.51 ±1.24 3.3418E-03 5.2560E-08 65725 61432 93.5% " 4,293 " 14 16 3 21 5 12 0 17 13 27 12 4 25 28 25 14 30 28 22 16 13 14 22 18 24 23 28 40 26 15 25 11 10 16 11 12 18 19 17 5 21 18 14 39 21 23 16 8 B6AxCF1/JGo original M 1 3 120594K "56,718" "±2,436" "57,399" "±4,761" 8.3% "47,271" 121.43 -1.379 ? 941205 35 17.5 445 0 1 Case 16. C57BL/6J female # 1 or 2 by BALB/c male #7 Hybrids generated at UT Memphis using Jackson Lab stock 11-Jan-95 3 "Box 35, Slot O-4" 2 "18,072 x" 146 x TK TK and RW "1,002 mm^2" "5,418 mm^2" 16.6 µm^2 0.49% 2850 14 282 20.1 ±6.02 ±1.67 1.0297E-02 1.8155E-07 48800 64000 131.1% " 15,200 " 26 16 15 21 22 15 17 18 22 23 33 8 25 21 replicate 0.00001 120594K "56,477" "±2,835" 5.0% "51,577" 109.50 Different thin section on same slot. 21-Apr-95 3 "Box 35, Slot O-4" "16,038 x" 194 x RW RW and RW "1,938 mm^2" "5,270 mm^2" 20.5 µm^2 0.91% 2517 23 516 22.4 ±5.28 ±1.13 32 17 15 26 25 21 15 24 26 25 19 25 20 29 15 15 24 29 19 28 16 28 23 B6AxCF1/JGo original M 1 3 120594L "69,454" "±5,199" "69,559" "±7,026" 10.1% "70,018" 99.34 0.780 ? 941205 35 19.3 454 0 1 Case 17. C57BL/6J female # 1 or 2 by BALB/c male #7 11-Jan-95 5 "Box 35, Slot P-1" "18,180 x" 146 x TK TK and RW "1,484 mm^2" "5,418 mm^2" 16.4 µm^2 0.49% 4271 21 342 16.3 ±7.36 ±1.64 2.6978E-03 3.8843E-08 71396 69858 97.8% " 1,538 " 32 20 29 19 20 15 21 14 10 14 15 24 5 13 7 12 2 18 22 12 18 replicate 0.00001 120594L "69,328" "±7,730" 11.2% "76,420" 90.72 Replicate by Rob of different thin section on same grid. Beautiful tissue. 13-Apr-95 5 "Box 35, Slot P-1" 4 "16,032 x" 191 x RW RW and RW "2,796 mm^2" "5,270 mm^2" 20.5 µm^2 0.54% 3727 20 372 18.6 ±9.04 ±2.07 21 15 27 9 12 11 10 18 20 17 31 24 20 33 8 7 27 3 30 29 B6AxCF1/JGo original M 1 3 120594M "65,825" "±4,151" "60,950" "±8,711" 14.3% "71,698" 85.01 0.165 ? 941205 35 19.3 458 0 1 Case 18. C57BL/6J female # 1 or 2 by BALB/c male #7 11-Jan-95 7 "Box 35, Slot Q-4" 3 "18,321 x" 197 x TK TK and RW "2,785 mm^2" "5,418 mm^2" 16.1 µm^2 0.41% 4442 18 247 13.7 ±8.09 ±1.96 4.0453E-03 6.1455E-08 68980 51857 75.2% " 17,123 " 2 12 21 20 20 17 20 14 2 28 20 3 5 9 9 13 7 25 replicate 0.00001 120594M "67,257" "±4,721" 7.0% "67,528" 99.60 Replicate by Rob of different thin section on same grid 13-Apr-95 7 "Box 35, Slot Q-4" "16,200 x" 248 x RW RW and RW "4,153 mm^2" "5,270 mm^2" 20.1 µm^2 0.68% 3363 23 460 20.0 ±6.59 ±1.40 18 16 28 8 25 17 8 16 19 17 23 37 16 23 20 24 21 24 15 14 22 30 19 B6AxCF1/JGo M 1 3 010695L "64,938" "±3,005" "64,938" "±3,005" 4.6% "80,594" 80.57 0.014 ? 941201 950106 36 20.5 512 0 2 Case 56. C57BL/6J females #1 or #2 by BALB/c male #7 21-Apr-95 3 "Box 42, Slot O-2" 4 "10,759 x" 194 x RW RW and RW "3,028 mm^2" "5,270 mm^2" 45.5 µm^2 1.24% 1770 22 807 36.7 ±7.78 ±1.70 7.5331E-03 1.1600E-07 31 42 56 44 36 30 31 30 30 29 35 39 52 39 44 33 30 33 37 46 32 28 B6AxCF1/JGo original M 1 3 010695M "74,205" "±5,236" "74,205" "±5,236" 7.1% "76,802" 96.62 1.586 ? 941201 950106 36 20.3 504 0 2 Case 57. C57BL/6J females #1 or #2 by BALB/c male #7 1 necrotic 5-May-95 5 "Box 42, Slot P-1" 3 "13,080 x" 206 x RW RW and RW "3,269 mm^2" "5,270 mm^2" 30.8 µm^2 0.84% 2493 21 625 29.8 ±9.39 ±2.10 2.8424E-03 3.8305E-08 40 43 33 36 40 18 22 28 31 39 11 28 22 32 43 30 10 27 27 36 29 B6AxCF1/JGo please reshoot 1E-08 010695M B6AxCF1/JGo Please photo M 010695N 941201 950106 36 17.0 498 0 2 Case 58. C57BL/6J females #1 or #2 by BALB/c male #7 6 "Box 42, Slot Q" B6AxCF1/JGo F 021395I 950107 950213 37 20.3 477 4 Case 126. C57BL/6J female # 1 or 2 by BALB/c male #7 B6AxCF1/JGo M 021395J 950107 950213 37 18.3 478 4 Case 127. C57BL/6J female # 1 or 2 by BALB/c male #7 B6AxCF1/JGo F 021395K 941101 950213 104 29.5 484 3 Case 28: Parents were B6 female 1 or 2 and BALB/c male #7 B6AxCF1/JGo F 021395L 941101 950213 104 27.0 509 3 Case 29: Parents were B6 female 1 or 2 and BALB/c male #7 B6AxCF1/JGo F 021395M 941101 950213 104 26.5 486 3 Case 30: Parents were B6 female 1 or 2 and BALB/c male #7 B6AxCF1/JGo F 030695N 941101 950306 125 26.5 505 5 Case 25 Agouti coat B6AxCF1/JGo F 030695O 941101 950306 125 28.0 484 5 Case 26 Agouti coat B6AxCF1/JGo F 030695P 941101 950306 125 25.0 480 5 Case 27 Agouti coat B6CF2/JGo F 020795J 453 950101 950207 37 19.8 480 1 or 2 Case 97. albino "Parents were B6CF1 males 21 and 22 and B6CF1 females 25, 26, and 27" "Tissue, not extracted" B6CF2/JGo F 020795K 950101 950207 37 17.3 439 1 or 2 Case 98. albino "Parents were B6CF1 males 21 and 22 and B6CF1 females 25, 26, and 27" "Tissue, not extracted" B6CF2/JGo F 020795L 950101 950207 37 17.0 421 1 or 2 Case 99. albino "Parents were B6CF1 males 21 and 22 and B6CF1 females 25, 26, and 27" "Tissue, not extracted" B6CF2/JGo F 020795M 950101 950207 37 16.0 461 1 or 2 Case 100. albino "Parents were B6CF1 males 21 and 22 and B6CF1 females 25, 26, and 27" "Tissue, not extracted" B6CF2/JGo F 020795N 950101 950207 37 17.5 451 1 or 2 Case 101. albino "Parents were B6CF1 males 21 and 22 and B6CF1 females 25, 26, and 27" "Tissue, not extracted" B6CF2/JGo F 020795O 950101 950207 37 14.5 402 1 or 2 Case 102. albino "Parents were B6CF1 males 21 and 22 and B6CF1 females 25, 26, and 27" "Tissue, not extracted" B6CF2/JGo F 020795P 950101 950207 37 18.5 455 1 or 2 Case 103. albino "Parents were B6CF1 males 21 and 22 and B6CF1 females 25, 26, and 27" "Tissue, not extracted" B6CF2/JGo F 020795Q 950101 950207 37 18.8 452 1 or 2 Case 104. agouti "Parents were B6CF1 males 21 and 22 and B6CF1 females 25, 26, and 27" "Tissue, not extracted" B6CF2/JGo F 020795R 950101 950207 37 17.0 477 1 or 2 Case 105. agouti "Parents were B6CF1 males 21 and 22 and B6CF1 females 25, 26, and 27" "Tissue, not extracted" B6CF2/JGo F 020795S 950101 950207 37 15.3 483 1 or 2 Case 106. agouti "Parents were B6CF1 males 21 and 22 and B6CF1 females 25, 26, and 27" "Tissue, not extracted" B6CF2/JGo F 020795T 950101 950207 37 20.0 486 1 or 2 Case 107. agouti "Parents were B6CF1 males 21 and 22 and B6CF1 females 25, 26, and 27" "Tissue, not extracted" B6CF2/JGo F 020795U 950101 950207 37 16.5 431 1 or 2 Case 108. agouti "Parents were B6CF1 males 21 and 22 and B6CF1 females 25, 26, and 27" "Tissue, not extracted" B6CF2/JGo M 020995A 950101 950209 39 21.5 480 1 or 2 Case 109. Albino "Parents were B6CF1 males 21 and 22 and B6CF1 females 25, 26, and 27" "Tissue, not extracted" B6CF2/JGo M 020995B 950101 950209 39 21.0 459 1 or 2 Case 110. Albino "Parents were B6CF1 males 21 and 22 and B6CF1 females 25, 26, and 27" "Tissue, not extracted" B6CF2/JGo M 020995C 950101 950209 39 22.5 483 1 or 2 Case 111. Albino "Parents were B6CF1 males 21 and 22 and B6CF1 females 25, 26, and 27" "Tissue, not extracted" B6CF2/JGo M 020995D 950101 950209 39 22.0 495 1 or 2 Case 112. Agouti "Parents were B6CF1 males 21 and 22 and B6CF1 females 25, 26, and 27" "Tissue, not extracted" B6CF2/JGo M 020995E 950101 950209 39 20.5 494 1 or 2 Case 113. Agouti "Parents were B6CF1 males 21 and 22 and B6CF1 females 25, 26, and 27" "Tissue, not extracted" B6CF2/JGo M 020995F 950101 950209 39 21.5 506 1 or 2 Case 114. Agouti "Parents were B6CF1 males 21 and 22 and B6CF1 females 25, 26, and 27" "Tissue, not extracted" B6CF2/JGo M 020995G 950101 950209 39 18.5 missing hindbrain 1 or 2 Case 115. Agouti. Part of brain missing. "Parents were B6CF1 males 21 and 22 and B6CF1 females 25, 26, and 27" "Tissue, not extracted" B6CF2/JGo M 020995H 950101 950209 39 21.8 478 1 or 2 Case 116. Agouti "Parents were B6CF1 males 21 and 22 and B6CF1 females 25, 26, and 27" "Tissue, not extracted" B6CF2/JGo M 020995I 950101 950209 39 18.5 462 1 or 2 Case 117. Black "Parents were B6CF1 males 21 and 22 and B6CF1 females 25, 26, and 27" "Tissue, not extracted" B6CF2/JGo F 021395A 950105 950213 39 19.0 472 3 Case 118 "Parents were B6CF1 males 21 and 22 and B6CF1 females 28, 29, and 30" "Tissue, not extracted" B6CF2/JGo M 021395B 950105 950213 39 24.8 486 3 Case 119 "Parents were B6CF1 males 21 and 22 and B6CF1 females 28, 29, and 30" "Tissue, not extracted" B6CF2/JGo F 021395C 950105 950213 39 18.0 472 3 Case 120 "Parents were B6CF1 males 21 and 22 and B6CF1 females 28, 29, and 30" "Tissue, not extracted" B6CF2/JGo F 021395D 950105 950213 39 18.3 450 3 Case 121 "Parents were B6CF1 males 21 and 22 and B6CF1 females 28, 29, and 30" "Tissue, not extracted" B6CF2/JGo M 021395E 950105 950213 39 22.3 474 3 Case 122 "Parents were B6CF1 males 21 and 22 and B6CF1 females 28, 29, and 30" "Tissue, not extracted" B6CF2/JGo F 021395F 950105 950213 39 17.8 455 3 Case 123 "Parents were B6CF1 males 21 and 22 and B6CF1 females 28, 29, and 30" "Tissue, not extracted" B6CF2/JGo M 021395G 950105 950213 39 23.3 506 3 Case 124 "Parents were B6CF1 males 21 and 22 and B6CF1 females 28, 29, and 30" "Tissue, not extracted" B6CF2/JGo M 021395H 950105 950213 39 20.0 467 3 Case 125 "Parents were B6CF1 males 21 and 22 and B6CF1 females 28, 29, and 30" "Tissue, not extracted" B6CF2/JGo M 030695D 950126 950306 39 20.0 439 4 Case 171. Albino "Parents were B6CF1 one female (25, 26, or 27) and male B6CF1 #21 or #22." "Tissue, not extracted" B6CF2/JGo M 030695E 950126 950306 39 19.0 445 4 Case 172. Albino "Parents were B6CF1 one female (25, 26, or 27) and male B6CF1 #21 or #22." "Tissue, not extracted" B6CF2/JGo F 030695F 950126 950306 39 17.0 436 4 Case 173. non-agouti "Parents were B6CF1 one female (25, 26, or 27) and male B6CF1 #21 or #22." "Tissue, not extracted" B6CF2/JGo M 030695G 950126 950306 39 17.0 440 4 Case 174 "Parents were B6CF1 one female (25, 26, or 27) and male B6CF1 #21 or #22." "Tissue, not extracted" B6CF2/JGo M 030695H 950126 950306 39 21.0 443 4 Case 175 "Parents were B6CF1 one female (25, 26, or 27) and male B6CF1 #21 or #22." "Tissue, not extracted" B6CF2/JGo M 030695I 950126 950306 39 21.0 456 4 Case 176 "Parents were B6CF1 one female (25, 26, or 27) and male B6CF1 #21 or #22." "Tissue, not extracted" B6CF2/JGo M 030695J 950126 950306 39 19.8 441 4 Case 177 "Parents were B6CF1 one female (25, 26, or 27) and male B6CF1 #21 or #22." "Tissue, not extracted" B6CF2/JGo F 030695K 950126 950306 39 16.0 399 4 Case 178 "Parents were B6CF1 one female (25, 26, or 27) and male B6CF1 #21 or #22." "Tissue, not extracted" B6CF2/JGo F 030695L 950126 950306 39 19.5 452 4 Case 179 "Parents were B6CF1 one female (25, 26, or 27) and male B6CF1 #21 or #22." "Tissue, not extracted" B6CF2/JGo M 030695M 950126 950306 39 19.5 431 4 Case 180 "Parents were B6CF1 one female (25, 26, or 27) and male B6CF1 #21 or #22." "Tissue, not extracted" BALB/cBy small sample at high mag F 1 1 120594N "57,558" "±1,768" "±4,330" 7 "57,402" "±6,978" "57,402" "±6,978" 12.2% "39,009" 147.15 -0.036 ? 421 941205 30 13.0 380 1 1 Photographed on JEOL 1000 All BALB/cBy from the Jackson Lab production colony. 1 necrotic on 0019 Jan-13-95 9 "Box 35, Slot R-1" 3 "18,151 x" 198 x TK RW and RW "1,535 mm^2" "5,418 mm^2" 16.4 µm^2 0.63% 2372 15 363 24.2 ±11.01 ±2.94 1.2630E-03 2.2002E-08 61589 62551 101.6% 962 44 28 12 18 40 25 17 14 15 39 20 22 10 22 37 BALB/cBy original M 1 1 120594O "59,816" "±3,978" "64,703" "±6,836" 10.6% "59,615" 108.54 0.522 ? 941205 30 15.5 408 0 1 Photographed on JEOL 1000 none Jan-13-95 12 "Box 35, Slot S-2" 4 "17,275 x" 198 x TK RW and RW "2,346 mm^2" "5,418 mm^2" 18.2 µm^2 0.52% 3283 17 335 19.7 ±8.33 ±2.08 4.0154E-03 6.7129E-08 65627 73707 112.3% " 8,080 " 17 15 9 26 18 9 22 21 15 31 32 29 18 10 25 7 31 BALB/cBy replicate 0.00001 120594O "57,313" "±4,892" 8.5% "62,918" 91.09 Replicate of same section none May-2-95 12 "Box 35, Slot S-2" "15,976 x" 247 x RW RW and RW "3,835 mm^2" "5,270 mm^2" 20.6 µm^2 0.69% 3047 21 395 18.8 ±7.18 ±1.61 20 23 13 30 22 16 16 22 31 19 19 7 21 23 17 18 28 6 24 4 16 BALB/cBy original F 1 1 120594P "63,924" "±1,894" "63,924" "±3,788" 5.9% "51,269" 124.68 1.470 ? 941205 30 14.0 413 1 1 Lots of unmyeliated fibers (20%) none Feb-28-95 13 "Box 38, Slot C-1" 4 "13,113 x" 194 x TK TK and RW "1,934 mm^2" "5,418 mm^2" 31.5 µm^2 1.72% 1627 28 1100 39.3 ±12.10 ±2.33 1.8476E-02 2.8903E-07 64709 63240 97.7% " 1,469 " 38 38 30 37 29 28 57 49 27 44 37 38 36 28 28 53 50 duplicate 40 45 22 66 55 24 56 50 44 17 34 BALB/cBy please reshoot 1E-08 120594P BALB/cBy F 1 1 120594R "55,038" "±2,981" "55,038" "±2,981" 5.4% "42,200" 130.42 -0.582 ? 941205 30 14.5 408 1 1 "Very nice tissue. Lots of unmyelinated fibers, that were very easy to count." none Feb-8-95 18 "Box 38, Slot A-5" 4 "13,113 x" 193 x TK TK and RW "1,572 mm^2" "5,418 mm^2" 31.5 µm^2 1.57% 1339 21 863 41.1 ±9.95 ±2.23 6.5030E-03 1.1816E-07 58504 55838 95.4% " 2,666 " 28 39 43 52 41 41 34 60 48 53 45 31 53 20 39 50 28 32 37 43 46 BALB/cBy original F 1 1 120594S "60,984" "±2,202" "57,652" "±5,006" 8.7% "56,682" 101.71 0.791 ? 941205 30 14.8 404 1 1 1 necrotic Mar-1-95 19 "Box 38, Slot D-2" 4 "13,113 x" 194 x TK TK and RW "2,138 mm^2" "5,418 mm^2" 31.5 µm^2 1.17% 1799 21 673 32.0 ±12.44 ±2.78 1.3208E-02 2.1657E-07 64006 54992 85.9% " 9,014 " 0 39 57 20 38 32 40 43 47 42 20 27 35 17 22 34 31 25 30 30 44 BALB/cBy replicate 0.00001 120594S "61,784" "±2,452" 4.0% "55,403" 111.52 Replicate of a new section. none 3-May-95 19 "Box 38, Slot D-2" "12,950 x" 245 x RW RW and RW "3,333 mm^2" "5,270 mm^2" 31.4 µm^2 1.25% 1763 22 771 35.0 ±6.37 ±1.39 40 26 36 37 34 24 35 35 35 37 31 38 45 19 34 42 40 45 33 33 31 41 BALB/cBy original M 1 1 010695E "51,320" "±1,984" "49,245" "±3,449" 7.0% "87,773" 56.11 -1.441 ? 940807 950106 152 27.5 471 0 1 #12. Confirmed BALB/cBy. No problems of identification. Both CAST/Ei processed in same batch have low numbers. Beautiful tissue with large axons. none 5-Apr-95 9 "Box 42, Slot B-2" 4 "13,066 x" 190 x RW RW and RW "3,166 mm^2" "5,270 mm^2" 30.9 µm^2 0.77% 2844 22 381 17.3 ±5.56 ±1.21 1.3653E-02 2.6604E-07 20 19 23 27 23 13 21 17 14 9 23 26 21 5 19 11 15 14 15 18 12 16 BALB/cBy replicate 0.00001 010695E "52,347" "±2,426" 4.6% "82,258" 63.64 Replicate of different thin section 5-May-95 9 "Box 42, Slot B-2" "10,848 x" 206 x RW RW and RW "3,481 mm^2" "5,270 mm^2" 44.8 µm^2 1.20% 1837 22 627 28.5 ±6.05 ±1.32 14 29 29 38 25 38 36 30 33 25 30 23 21 33 20 23 34 31 28 33 26 28 BALB/cBy F 1 1 010695D "54,424" "±2,374" "54,424" "±2,374" 4.4% "82,356" 66.08 -0.724 ? 940807 950106 152 25.5 464 1 1 #11 Also cut beautiful tissue Apr-21-95 7 "Box 42, Slot A-1" 4 "12,802 x" 192 x RW RW and RW "3,052 mm^2" "5,270 mm^2" 32.2 µm^2 0.94% 2561 24 510 21.3 ±4.45 ±0.93 1.0077E-02 1.8516E-07 25 21 22 27 21 21 16 23 20 17 30 19 24 26 15 22 11 19 19 22 27 23 15 25 BALB/cBy bad block M 120594Q 941205 30 14.8 404 1 "First session of Feb 8, 95 no good. Section of nerve incomplete. Recut by Richelle, but still ragged." Feb-9-95 16 "Box 38, Slot B-4" TK BALB/cBy-mceph/+ M 5 071095A-2 463 941114 950710 238 NA 454 2 #1295 "N13, megaencephaly heterozygote from Cook. Unmapped locus." BALB/cBy-mceph/+ F 5 071095C-2 950409 950710 92 NA 468 1 #1334 N13F1. megaencephaly heterozygote BALB/cBy-mceph/+ F 5 071095E-2 950409 950710 92 NA 457 1 #1335 N13F1. megaencephaly heterozygote BALB/cBy-mceph/+ F 5 072595F-2 950505 950725 81 27.0 477 2 #1337 Megaencephaly heterozygote BALB/cBy-mceph/+ F 5 072595H-2 950507 950725 79 23.5 460 4 #1339 Megaencephaly heterozygote BALB/cBy-mceph/mceph F 5 071095B-2 545 950409 950710 92 NA 542 1 #1332 N13F1. megaencephaly homozygote BALB/cBy-mceph/mceph F 5 071095D-2 950409 950710 92 NA 550 1 #1333 N13F1. megaencephaly homozygote BALB/cBy-mceph/mceph F 5 072595E-2 "61,074" "±5,338" "61,074" "±5,338" 8.7% "108,741" 56.16 950505 950725 81 19.8 541 1 2 #1336 "Megaencephaly homozygote. Tissue is not pretty, but easy to count. Few unmyelinated fibers." none Sep-19-95 10 "Box 54, Slot A-1" 2 "12,872 x" 190 x RCS RCS and RW "3,945 mm^2" "5,324 mm^2" 32.1 µm^2 0.65% 3384 22 397 18.0 ±7.23 ±1.58 2.2456E-03 3.6769E-08 22 8 24 21 12 8 18 22 21 29 19 7 12 19 35 11 8 20 18 24 22 17 BALB/cBy-mceph/mceph F 5 072595G-2 950507 950725 79 20.0 549 3 #1338 Megaencephaly homozygote BALB/cBY.AKR congenic NM1360 M 5 062592E "40,395" "±2,336" "40,395" "±2,336" 5.8% "59,460" 67.94 Jackson New Mutant 1360 JAX1151 L 432 920423 920625 63 21.0 400 0 1 NM= new mutation at Jackson Laboratory from BALB/cBy.AKR cog stock. Mutant goes from side to side. N10F1 +13 All of these congenics and new mutants from the Jackson Lab Research colony. photo#22 is bad Aug-12-92 C2 "Box 16, Slot A-1" "13,158 x" 509 x LN 8-5-92 JO and JO "15,405 mm^2" "5,324 mm^2" 30.7 µm^2 1.40% 1934 27 564 20.9 ±6.16 ±1.21 7.6855E-03 1.9026E-07 40163 40610 101.1% 446 30 12 24 26 26 18 18 15 25 19 21 18 24 31 20 6 19 30 21 23 23 25 10 19 13 28 20 BALB/cBY.AKR-nm/nm F 081094A Jackson New Mutant 1360 JAX1154 930527 940810 440 23.0 408 2 Done at Jackson. An albino that looks sickly and old. Has a motor deficit. Died after injection. Perfused with 3.5% paraform with 0.1% glut. BALB/cBY.AKR-+/nm F 081094B Jackson New Mutant 1360 JAX1134 940330 940810 133 31.0 476 3 Done at JAX. Good perfusion. HRP injection. Normal heterozygote. BALB/cBY.AKR-+/nm F 081094C Jackson New Mutant 1360 JAX1135 940330 940810 133 31.0 457 3 Done at JAX. Good perfusion. HRP injection. Normal heterozygote. BALB/cBY.AKR-nm/nm M 081094D Jackson New Mutant 1360 JAX1136 940330 940810 133 23.0 418 3 "Done at JAX. Good perfusion. HRP injection. Mutant with smaller body, slow writhing response, normal pupillary light reflex." BALB/cBy.C57BL/6By-+ Hbb/+ Hbb F 5 062692C "69,555" "±6,204" "69,555" "±6,204" 8.9% "77,916" 89.27 JR0899 JAX525 ? 920627 100 19.0 434 1 "Brown agouti, BALB C.B6-+ Hbb/+ Hbb labeled Pigmented BALB/c. I don't trust the low calibration. It is way out of focus." 9-Mar-93 B2 "Box 20A, Slot D-2" 3 "12,857 x" 176 x DR DR and RW "2,414 mm^2" "5,313 mm^2" 32.1 µm^2 1.20% 2424 29 832 28.7 ±13.54 ±2.56 1.8716E-03 2.6907E-08 63898 74831 117.1% " 10,933 " 65 36 39 44 37 37 30 41 34 18 24 30 25 22 9 28 57 28 32 24 16 11 22 16 17 27 14 7 42 BALB/cJ F 1 1 112292C "63,393" "±2,210" "±5,846" 8 "68,149" "±3,016" "68,149" "±3,016" 4.4% "81,628" "3,310" 541 83.49 0.814 "54,683 " JR000651 L 477 920916 921123 68 477 13.9 1 1 Reshot montage and low mag calibration 1 necrotic axon on 0027 Jan-12-93 D3 "Box 21, Slot LÐ1" 4 "11,124 x" 250 x DR DR and RW "5,091 mm^2" "5,237 mm^2" 41.2 µm^2 1.66% 1983 33 1134 34.4 ±8.60 ±1.52 7.7283E-03 1.1340E-07 66684 65245 97.8% " 1,439 " 40 37 27 32 39 52 20 43 46 40 49 38 28 25 38 39 32 20 43 37 30 19 21 47 41 31 33 31 35 34 33 31 23 BALB/cJ F 1 1 112292D "67,477" "±3,169" "67,477" "±3,169" 4.7% "68,428" 98.61 0.699 "64,451 " JR000651 L 920916 921123 68 19.0 449 15.3 1 1 Great HRP backfill "1 or 2 necrotic axons in series, see 0041and 0015" Jan-12-93 C1 "Box 21, Slot E-1" 3 "13,347 x" 198 x DR DR and RW "2,685 mm^2" "5,237 mm^2" 28.9 µm^2 1.35% 2370 32 911 28.5 ±7.44 ±1.34 6.9355E-03 1.0278E-07 67105 67845 101.1% 741 20 34 37 20 26 29 15 33 22 35 23 21 34 37 32 22 27 10 32 31 28 31 34 26 34 31 36 15 25 39 33 39 BALB/cJ original M 1 1 011994E "63,529" "±2,441" "57,191" "±3,174" 5.5% "75,261" 75.99 0.023 JR000651 ? 940119 66 26.3 489 0 2 1 large necrotic axon 0009 Mar-31-94 1 "Box 26, Slot A-1" 3 "12,984 x" 194 x TK TK and RW "2,832 mm^2" "5,435 mm^2" 32.2 µm^2 1.03% 2334 24 588 24.5 ±6.52 ±1.36 1.1124E-02 1.7511E-07 58329 60347 103.5% " 2,018 " 20 30 27 26 36 32 24 28 18 31 29 15 20 22 34 30 21 18 27 16 31 18 12 23 BALB/cJ replicate 0.00001 011994E "60,295" "±3,819" 6.3% "74,556" 80.87 Replicate to confirm outlier status. Apr-17-95 1 "Box 26, Slot A-1" "13,096 x" 193 x RW RW and RW "2,782 mm^2" "5,270 mm^2" 30.7 µm^2 0.82% 2426 20 497 24.9 ±6.86 ±1.57 22 24 21 26 24 30 20 20 27 7 36 33 37 28 27 21 26 17 21 30 BALB/cJ other side 011994E "68,599" "±4,332" 6.3% "89,636" 76.53 Other side analyzed by Stacie none Jul-11-95 2 "Box 50, Slot A-1" 4 "12,877 x" 194 x SS SS and SS and RW "3,373 mm^2" "5,270 mm^2" 31.8 µm^2 1.21% 2820 34 827 24.3 ±8.82 ±1.54 10 20 13 21 33 31 28 28 29 29 16 21 26 8 18 21 17 52 19 25 26 30 16 13 19 28 23 19 30 41 31 28 34 24 BALB/cJ please reshoot this high side again 011994E BALB/cJ original M 1 1 011994F "53,252" "±2,743" "53,8