Project 1: The Mouse Brain Library Project 2: Internet Microscopy (iScope) Project 3: Neurocartographer and Segmentation of the MBL Project 4: The Neurogenetics Tool Box


















Principal Investigator/Program Director Williams,Robert W.

  Aim 4: Streaming video.

Streaming video technology is developing extraordinarily rapidly. In Year 01 we will apply Apple Computers QuickTime 4 (QT4) streaming server technology to the existing iScope. The microscope is already in place with all of the necessary computer hardware and software. The QT4 architecture has an impressive feature set, and we will be able to simultaneously deliver streams of color video at multiple resolutions. The QT4 streaming system will allow us to deliver at least 10 frames per sec (fps) at a resolution of 160 x 120 pixels, even to clients on 56 Kbps connections. At the other end of the spectrum, the full digital video camera output amounts to 3.6 MB/sec, and this bandwidth will become widely available in the next decade. We expect to eventually serve the original digital video from our archive or directly from the cameras. The main advantage of streaming video is that Internet microscopists will be able to use the collection in a more natural way that would otherwise be possible. For example, they will be able to record their own series or montages of images from any brain in the MBL; they can search for rare features such as dying neurons or mitotic figures; or they can define the precise coordinates of sites that they would like us to acquire on their behalf using our rapid through-focus image acquisition batch processing system.
Letters in the appendix emphasize the enthusiasm of our research and network administrators at UT Memphis for the Internet microscopy lab. They view this project as a powerful teaching tool, and they guarantee their full cooperation in ensuring that our servers will have optimized connections to the Internet. They have also offered new space and substantial funds for the duration of the grant to purchase and upgrade computer equipment and software

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