Project 1: The Mouse Brain Library Project 2: Internet Microscopy (iScope) Project 3: Neurocartographer and Segmentation of the MBL Project 4: The Neurogenetics Tool Box


















Principal Investigator/Program Director Williams,Robert W.


The experiments, tests, and development proposed in this application will take five years. Because the iScope project is not hypothesis-driven, this section of the project appears to have an open-ended character. However, we have specific and well-defined aims, and given our accomplishments to date, we believe that within five years we can refine and apply the technology for highly effective Internet microscopy. Furthermore, this is enough time to gather a substantial collection of through-focus image stacks. These stacks will be a major component of the MBL and will make available an excellent collection of stereological material from hundreds of strains of mice. In addition, we will make our very large QTL mapping cross available to the genetics, neurogenetics, and neuroscience communities.

Hazardous procedures or material.

The experiments outlined in this application do not involve hazardous materials or procedures.

General Comments

We will build four new iScope systems during the first four years of this grant. The first two microscopes will explore important different hardware options, foremost, the utility of inverted versus. fixed-stage upright microscope stands.
The remaining parts of Section 4 describe individual specific aims. Throughout this section we have made a number of references to current web resources, both our own and those of other groups in the format <> because the prefix http:// does not have to be entered into current browsers.


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  Aim 1: IScope Microscope Design and Assembly.