Text file started Oct 10, 1998
The original protocol came from (I think) a BioTechniques paper based on typing people for cystic fibrosis mutations with blood spots. Our in-house protocol is quite different from this:
Fixed DNA samples are now ready for PCR. To amplify punch out 2 mm holes (an ear punch works well) into PCR tubes. Add 10 µl water and heat for 5 minutes at 95 deg C, add PCR components and amplify. Others in the lab have eliminated the preheat step.
Two words of caution with this protocol. First, as with ear punch methods, there is cross contamination if you choose not to clean the punch between samples so this method can be a pain for typing in presence vs absence screens. Second, we found this method successful with radiolabled PCR assays, but the sensitivity with agarose typings was lower, but still functional with most primer pairs.