Project 1: The Mouse Brain Library Project 2: Internet Microscopy (iScope) Project 3: Neurocartographer and Segmentation of the MBL Project 4: The Neurogenetics Tool Box


















Principal Investigator/Program Director Williams,Robert W.


Aim 2: Affined mapping into standard coordinates

Each brain in the MBL is stored as a series of ordered but unaligned images of cresyl violetstained sections interlaced with Loyez fiberstained sections. In-plane image resolution now ranges from 25 to 4.5 m; off-plane sampling for a given stain is one section in five (150 mm). To exploit this vast image database efficiently, we will compute affine transformations that best aligns each section to the MBL coordinate system. This registration process will allow neuroscientists using NeuroTerrain to find homologous planes of view across the brains stored in the database at a resolution of approximately 225 mm. NeuroTerrain will be enhanced to support viewing matched planes from multiple brains simultaneously. New alignment software will also be made available to users for registering a 2D section set into a 3D atlas.



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