Project 1: The Mouse Brain Library Project 2: Internet Microscopy (iScope) Project 3: Neurocartographer and Segmentation of the MBL Project 4: The Neurogenetics Tool Box

 

 

 

 























 

 

 

 

 

 

  

 

  
 

RESEARCH PLAN

  
 

Principal Investigator/Program Director Williams, Robert W.

 
 

Aim 1. Extend the MBL.

The current MBL contains images of celloidin-embedded, Nissl-stained brains representing over 100 different strains. The brains were imaged at both 25 µm/pixel and .5 µm/pixel resolution. During the course of this proposal, we will extend and diversify this collection, adding recombinant inbred (RI) strains to complete our collection of BXD, AXB, and BXA sets. We will include over 600 mice from the tenth-generation advanced intercross (the G10 set. see Core B) and will complete the collection of standard inbred strains. We will also increase the depth of this resource by adding more images (1 µm/pixel) to the database, representing 40-50 anatomical structures of interest to the neuroscience community. We will begin to include immunohistochemically stained tissue and will stain adjacent sections from the celloidin collection for complementary information.

 

  
   
   
   
 

NEXT TOPIC

  
 

Aim 2. Generate a Relational Database for the MBL