Project 1: The Mouse Brain Library Project 2: Internet Microscopy (iScope) Project 3: Neurocartographer and Segmentation of the MBL Project 4: The Neurogenetics Tool Box


















Principal Investigator/Program Director Williams,Robert W.

  Streaming video.

There are numerous special requirements for effective streaming microscopy on the web. Not only must the video system provide a rapid update from the camera, but the web microscopist needs to be able to rapidly control the movement of the stage. Achieving fluid two-way communication is a challenge. For example, in our current implementation, the duty cycle of the systemthat is, the time between initiating a request to move the stage and the time at which the video is actually refreshed on the clients web browseris approximately 1020 seconds, even on an in-house connection. This lag is primarily due to the fact that we have not yet implemented a streaming video server. The current system is a standard web server that orders new video frames from the camera at 10-second intervals. For true streaming video, faster low-resolution video is preferable to slow high-resolution video during the navigational stage. A relatively large field of view is also preferable to a small high-magnification view. For this reason, each microscope used for streaming video will be equipped with two video ports and two digital cameras. One camera will be optimized for navigation and the other for analysis and image capture.

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  Future Directions.